Result

Search results for the GEO ID: GSE25178

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM618385

GPL1261

midbrain floor plate_1

E10.5 embryo midbrain floor plate

strain: C57BL/6 gender: mix age: embryonic 10.5 days region: floor plate

Sample_geo_accession	GSM618385
Sample_status	Public on May 18 2011
Sample_submission_date	Nov 05 2010
Sample_last_update_date	May 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from the aforementioned replicate groups was prepared using the Stratagene MicroRNA kit as per manufacturer’s instructions with the exception that DNase treatment was extended to 30 minutes and RNAs were resuspended in RNase-free water.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5ng of total RNA in conjunction with the Nugen Ovation V2 amplification system (www.nugeninc.com) was used for preparation of the biotin-labeled cell extract. The efficacy of conversion of total RNA to labeled cDNA was monitored by the inclusion of Affymetrix polyA controls (www.affymetrix.com).
Sample_hyb_protocol	7 ug of biotin-labeled cell extract was hybridized to Affymetrix Mouse Genome 430_2 GeneChips (representing approximately 39,000 transcripts) for a period of 20 hours. The relative level of hybridization to the GeneChip was assessed using the Affymetrix hybridization controls. The hybridized arrays were washed, stained and scanned in accordance with the appropriate Nugen and Affymetrix guide lines.
Sample_scan_protocol	The hybridized arrays were washed, stained and scanned in accordance with the appropriate Nugen and Affymetrix guide lines.
Sample_data_processing	Microarray data in CEL format were analysed using GeneSpring GX11 program. Robust Multi-Array Analysis (RMA) Summarization Algorithm was used for normalisation. Baseline was transformed to Median of all samples. Both 'Raw signal values' and 'Normalized signal values' were exported as text files.
Sample_platform_id	GPL1261
Sample_contact_name	Xinsheng,,Nan
Sample_contact_email	xinsheng.nan@csc.mrc.ac.uk
Sample_contact_laboratory	Stem Cell Neurogenesis
Sample_contact_institute	MRC Clinical Science Centre
Sample_contact_address	Hammersmith Hospital
Sample_contact_city	London
Sample_contact_state	England
Sample_contact_zip/postal_code	W12 0NN
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618385/suppl/GSM618385.CEL.gz
Sample_series_id	GSE25178
Sample_data_row_count	45101

GSM618386

GPL1261

midbrain floor plate_2

E10.5 embryo midbrain floor plate

strain: C57BL/6 gender: mix age: embryonic 10.5 days region: floor plate

Sample_geo_accession	GSM618386
Sample_status	Public on May 18 2011
Sample_submission_date	Nov 05 2010
Sample_last_update_date	May 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA from the aforementioned replicate groups was prepared using the Stratagene MicroRNA kit as per manufacturer’s instructions with the exception that DNase treatment was extended to 30 minutes and RNAs were resuspended in RNase-free water.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5ng of total RNA in conjunction with the Nugen Ovation V2 amplification system (www.nugeninc.com) was used for preparation of the biotin-labeled cell extract. The efficacy of conversion of total RNA to labeled cDNA was monitored by the inclusion of Affymetrix polyA controls (www.affymetrix.com).
Sample_hyb_protocol	7 ug of biotin-labeled cell extract was hybridized to Affymetrix Mouse Genome 430_2 GeneChips (representing approximately 39,000 transcripts) for a period of 20 hours. The relative level of hybridization to the GeneChip was assessed using the Affymetrix hybridization controls. The hybridized arrays were washed, stained and scanned in accordance with the appropriate Nugen and Affymetrix guide lines.
Sample_scan_protocol	The hybridized arrays were washed, stained and scanned in accordance with the appropriate Nugen and Affymetrix guide lines.
Sample_data_processing	Microarray data in CEL format were analysed using GeneSpring GX11 program. Robust Multi-Array Analysis (RMA) Summarization Algorithm was used for normalisation. Baseline was transformed to Median of all samples. Both 'Raw signal values' and 'Normalized signal values' were exported as text files.
Sample_platform_id	GPL1261
Sample_contact_name	Xinsheng,,Nan
Sample_contact_email	xinsheng.nan@csc.mrc.ac.uk
Sample_contact_laboratory	Stem Cell Neurogenesis
Sample_contact_institute	MRC Clinical Science Centre
Sample_contact_address	Hammersmith Hospital
Sample_contact_city	London
Sample_contact_state	England
Sample_contact_zip/postal_code	W12 0NN
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618386/suppl/GSM618386.CEL.gz
Sample_series_id	GSE25178
Sample_data_row_count	45101

GSM618387

GPL1261

midbrain floor plate_3

E10.5 embryo midbrain floor plate

strain: C57BL/6 gender: mix age: embryonic 10.5 days region: floor plate

Sample_geo_accession	GSM618387
Sample_status	Public on May 18 2011
Sample_submission_date	Nov 06 2010
Sample_last_update_date	May 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared using the Stratagene MicroRNA kit as per manufacturer’s instructions with the exception that DNase treatment was extended to 30 minutes and RNAs were resuspended in RNase-free water.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5ng of total RNA in conjunction with the Nugen Ovation V2 amplification system (www.nugeninc.com) was used for preparation of the biotin-labeled cell extract. The efficacy of conversion of total RNA to labeled cDNA was monitored by the inclusion of Affymetrix polyA controls (www.affymetrix.com).
Sample_hyb_protocol	7 ug of biotin-labeled cell extract was hybridized to Affymetrix Mouse Genome 430_2 GeneChips (representing approximately 39,000 transcripts) for a period of 20 hours. The relative level of hybridization to the GeneChip was assessed using the Affymetrix hybridization controls.
Sample_scan_protocol	The hybridized arrays were washed, stained and scanned in accordance with the appropriate Nugen and Affymetrix guide lines.
Sample_data_processing	Microarray data in CEL format were analysed using GeneSpring GX11 program. Robust Multi-Array Analysis (RMA) Summarization Algorithm was used for normalisation. Baseline was transformed to Median of all samples. Both 'Raw signal values' and 'Normalized signal values' were exported as text files.
Sample_platform_id	GPL1261
Sample_contact_name	Xinsheng,,Nan
Sample_contact_email	xinsheng.nan@csc.mrc.ac.uk
Sample_contact_laboratory	Stem Cell Neurogenesis
Sample_contact_institute	MRC Clinical Science Centre
Sample_contact_address	Hammersmith Hospital
Sample_contact_city	London
Sample_contact_state	England
Sample_contact_zip/postal_code	W12 0NN
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618387/suppl/GSM618387.CEL.gz
Sample_series_id	GSE25178
Sample_data_row_count	45101

GSM618388

GPL1261

midbrain ventral lateral_1

E10.5 embryo midbrain ventral lateral region

strain: C57BL/6 gender: mix age: embryonic 10.5 days region: ventral lateral region

Sample_geo_accession	GSM618388
Sample_status	Public on May 18 2011
Sample_submission_date	Nov 06 2010
Sample_last_update_date	May 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared using the Stratagene MicroRNA kit as per manufacturer’s instructions with the exception that DNase treatment was extended to 30 minutes and RNAs were resuspended in RNase-free water.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5ng of total RNA in conjunction with the Nugen Ovation V2 amplification system (www.nugeninc.com) was used for preparation of the biotin-labeled cell extract. The efficacy of conversion of total RNA to labeled cDNA was monitored by the inclusion of Affymetrix polyA controls (www.affymetrix.com).
Sample_hyb_protocol	7 ug of biotin-labeled cell extract was hybridized to Affymetrix Mouse Genome 430_2 GeneChips (representing approximately 39,000 transcripts) for a period of 20 hours. The relative level of hybridization to the GeneChip was assessed using the Affymetrix hybridization controls.
Sample_scan_protocol	The hybridized arrays were washed, stained and scanned in accordance with the appropriate Nugen and Affymetrix guide lines.
Sample_data_processing	Microarray data in CEL format were analysed using GeneSpring GX11 program. Robust Multi-Array Analysis (RMA) Summarization Algorithm was used for normalisation. Baseline was transformed to Median of all samples. Both 'Raw signal values' and 'Normalized signal values' were exported as text files.
Sample_platform_id	GPL1261
Sample_contact_name	Xinsheng,,Nan
Sample_contact_email	xinsheng.nan@csc.mrc.ac.uk
Sample_contact_laboratory	Stem Cell Neurogenesis
Sample_contact_institute	MRC Clinical Science Centre
Sample_contact_address	Hammersmith Hospital
Sample_contact_city	London
Sample_contact_state	England
Sample_contact_zip/postal_code	W12 0NN
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618388/suppl/GSM618388.CEL.gz
Sample_series_id	GSE25178
Sample_data_row_count	45101

GSM618389

GPL1261

midbrain ventral lateral_2

E10.5 embryo midbrain ventral lateral region

strain: C57BL/6 gender: mix age: embryonic 10.5 days region: ventral lateral region

Sample_geo_accession	GSM618389
Sample_status	Public on May 18 2011
Sample_submission_date	Nov 06 2010
Sample_last_update_date	May 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared using the Stratagene MicroRNA kit as per manufacturer’s instructions with the exception that DNase treatment was extended to 30 minutes and RNAs were resuspended in RNase-free water.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5ng of total RNA in conjunction with the Nugen Ovation V2 amplification system (www.nugeninc.com) was used for preparation of the biotin-labeled cell extract. The efficacy of conversion of total RNA to labeled cDNA was monitored by the inclusion of Affymetrix polyA controls (www.affymetrix.com).
Sample_hyb_protocol	7 ug of biotin-labeled cell extract was hybridized to Affymetrix Mouse Genome 430_2 GeneChips (representing approximately 39,000 transcripts) for a period of 20 hours. The relative level of hybridization to the GeneChip was assessed using the Affymetrix hybridization controls.
Sample_scan_protocol	The hybridized arrays were washed, stained and scanned in accordance with the appropriate Nugen and Affymetrix guide lines.
Sample_data_processing	Microarray data in CEL format were analysed using GeneSpring GX11 program. Robust Multi-Array Analysis (RMA) Summarization Algorithm was used for normalisation. Baseline was transformed to Median of all samples. Both 'Raw signal values' and 'Normalized signal values' were exported as text files.
Sample_platform_id	GPL1261
Sample_contact_name	Xinsheng,,Nan
Sample_contact_email	xinsheng.nan@csc.mrc.ac.uk
Sample_contact_laboratory	Stem Cell Neurogenesis
Sample_contact_institute	MRC Clinical Science Centre
Sample_contact_address	Hammersmith Hospital
Sample_contact_city	London
Sample_contact_state	England
Sample_contact_zip/postal_code	W12 0NN
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618389/suppl/GSM618389.CEL.gz
Sample_series_id	GSE25178
Sample_data_row_count	45101

GSM618390

GPL1261

midbrain ventral lateral_3

E10.5 embryo midbrain ventral lateral region

strain: C57BL/6 gender: mix age: embryonic 10.5 days region: ventral lateral region

Sample_geo_accession	GSM618390
Sample_status	Public on May 18 2011
Sample_submission_date	Nov 06 2010
Sample_last_update_date	May 18 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared using the Stratagene MicroRNA kit as per manufacturer’s instructions with the exception that DNase treatment was extended to 30 minutes and RNAs were resuspended in RNase-free water.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5ng of total RNA in conjunction with the Nugen Ovation V2 amplification system (www.nugeninc.com) was used for preparation of the biotin-labeled cell extract. The efficacy of conversion of total RNA to labeled cDNA was monitored by the inclusion of Affymetrix polyA controls (www.affymetrix.com).
Sample_hyb_protocol	7 ug of biotin-labeled cell extract was hybridized to Affymetrix Mouse Genome 430_2 GeneChips (representing approximately 39,000 transcripts) for a period of 20 hours. The relative level of hybridization to the GeneChip was assessed using the Affymetrix hybridization controls.
Sample_scan_protocol	The hybridized arrays were washed, stained and scanned in accordance with the appropriate Nugen and Affymetrix guide lines.
Sample_data_processing	Microarray data in CEL format were analysed using GeneSpring GX11 program. Robust Multi-Array Analysis (RMA) Summarization Algorithm was used for normalisation. Baseline was transformed to Median of all samples. Both 'Raw signal values' and 'Normalized signal values' were exported as text files.
Sample_platform_id	GPL1261
Sample_contact_name	Xinsheng,,Nan
Sample_contact_email	xinsheng.nan@csc.mrc.ac.uk
Sample_contact_laboratory	Stem Cell Neurogenesis
Sample_contact_institute	MRC Clinical Science Centre
Sample_contact_address	Hammersmith Hospital
Sample_contact_city	London
Sample_contact_state	England
Sample_contact_zip/postal_code	W12 0NN
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618390/suppl/GSM618390.CEL.gz
Sample_series_id	GSE25178
Sample_data_row_count	45101

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