Search results for the GEO ID: GSE25191  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM618720 | GPL570 |
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non-SP cell, biological replicate1
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IGROV1, non-SP cell
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cell type: non-side population cell
cell line source: IGROV1
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Gene expression data from IGROV1 non-SP cell
|
| Sample_geo_accession | GSM618720
| | Sample_status | Public on Feb 09 2011
| | Sample_submission_date | Nov 08 2010
| | Sample_last_update_date | Feb 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Hoechst 33342 cell sorting
| | Sample_growth_protocol_ch1 | IGROV1 cell line was maintained in RPMI 1640 (Invitrogen, Paisley, UK) containing 10% fetal bovine serum (PAA Laboratories, Yeovil, UK) in humidified 5% CO2 in air incubators at 37 ̊C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells isolated by FACS and RNA isolated using Arcturus Pico Pure Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide)
| | Sample_hyb_protocol | The NuGEN hybtidsation v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual
| | Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| | Sample_data_processing | The data were analyzed in R (version 2.8.1) using Affy package and RMA as the normalisation method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei,,Dai
| | Sample_contact_email | w.dai@imperial.ac.uk
| | Sample_contact_laboratory | Epigenetics
| | Sample_contact_department | Sugery and Cancer
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618720/suppl/GSM618720_1207_SK_3_4_H_IGNSP1.CEL.gz
| | Sample_series_id | GSE25191
| | Sample_data_row_count | 54675
| | |
|
GSM618721 | GPL570 |
|
non-SP cell, biological replicate2
|
IGROV1, non-SP cell
|
cell type: non-side population cell
cell line source: IGROV1
|
Gene expression data from IGROV1 non-SP cell
|
| Sample_geo_accession | GSM618721
| | Sample_status | Public on Feb 09 2011
| | Sample_submission_date | Nov 08 2010
| | Sample_last_update_date | Feb 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Hoechst 33342 cell sorting
| | Sample_growth_protocol_ch1 | IGROV1 cell line was maintained in RPMI 1640 (Invitrogen, Paisley, UK) containing 10% fetal bovine serum (PAA Laboratories, Yeovil, UK) in humidified 5% CO2 in air incubators at 37 ̊C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells isolated by FACS and RNA isolated using Arcturus Pico Pure Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide)
| | Sample_hyb_protocol | The NuGEN hybtidsation v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual
| | Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| | Sample_data_processing | The data were analyzed in R (version 2.8.1) using Affy package and RMA as the normalisation method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei,,Dai
| | Sample_contact_email | w.dai@imperial.ac.uk
| | Sample_contact_laboratory | Epigenetics
| | Sample_contact_department | Sugery and Cancer
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618721/suppl/GSM618721_1207_SK_3_9_H_IGNSP2.CEL.gz
| | Sample_series_id | GSE25191
| | Sample_data_row_count | 54675
| | |
|
GSM618722 | GPL570 |
|
non-SP cell, biological replicate3
|
IGROV1, non-SP cell
|
cell type: non-side population cell
cell line source: IGROV1
|
Gene expression data from IGROV1 non-SP cell
|
| Sample_geo_accession | GSM618722
| | Sample_status | Public on Feb 09 2011
| | Sample_submission_date | Nov 08 2010
| | Sample_last_update_date | Feb 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Hoechst 33342 cell sorting
| | Sample_growth_protocol_ch1 | IGROV1 cell line was maintained in RPMI 1640 (Invitrogen, Paisley, UK) containing 10% fetal bovine serum (PAA Laboratories, Yeovil, UK) in humidified 5% CO2 in air incubators at 37 ̊C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells isolated by FACS and RNA isolated using Arcturus Pico Pure Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide)
| | Sample_hyb_protocol | The NuGEN hybtidsation v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual
| | Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| | Sample_data_processing | The data were analyzed in R (version 2.8.1) using Affy package and RMA as the normalisation method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei,,Dai
| | Sample_contact_email | w.dai@imperial.ac.uk
| | Sample_contact_laboratory | Epigenetics
| | Sample_contact_department | Sugery and Cancer
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618722/suppl/GSM618722_1207_SK_3_6_H_IGNSP3.CEL.gz
| | Sample_series_id | GSE25191
| | Sample_data_row_count | 54675
| | |
|
GSM618723 | GPL570 |
|
SP cell, biological replicate1
|
IGROV1, SP cell
|
cell type: side population cell
cell line source: IGROV1
|
Gene expression data from IGROV1 SP cell
|
| Sample_geo_accession | GSM618723
| | Sample_status | Public on Feb 09 2011
| | Sample_submission_date | Nov 08 2010
| | Sample_last_update_date | Feb 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Hoechst 33342 cell sorting
| | Sample_growth_protocol_ch1 | IGROV1 cell line was maintained in RPMI 1640 (Invitrogen, Paisley, UK) containing 10% fetal bovine serum (PAA Laboratories, Yeovil, UK) in humidified 5% CO2 in air incubators at 37 ̊C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells isolated by FACS and RNA isolated using Arcturus Pico Pure Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide)
| | Sample_hyb_protocol | The NuGEN hybtidsation v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual
| | Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| | Sample_data_processing | The data were analyzed in R (version 2.8.1) using Affy package and RMA as the normalisation method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei,,Dai
| | Sample_contact_email | w.dai@imperial.ac.uk
| | Sample_contact_laboratory | Epigenetics
| | Sample_contact_department | Sugery and Cancer
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618723/suppl/GSM618723_1207_SK_3_1_H_IGSP1.CEL.gz
| | Sample_series_id | GSE25191
| | Sample_data_row_count | 54675
| | |
|
GSM618724 | GPL570 |
|
SP cell, biological replicate2
|
IGROV1, SP cell
|
cell type: side population cell
cell line source: IGROV1
|
Gene expression data from IGROV1 SP cell
|
| Sample_geo_accession | GSM618724
| | Sample_status | Public on Feb 09 2011
| | Sample_submission_date | Nov 08 2010
| | Sample_last_update_date | Feb 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Hoechst 33342 cell sorting
| | Sample_growth_protocol_ch1 | IGROV1 cell line was maintained in RPMI 1640 (Invitrogen, Paisley, UK) containing 10% fetal bovine serum (PAA Laboratories, Yeovil, UK) in humidified 5% CO2 in air incubators at 37 ̊C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells isolated by FACS and RNA isolated using Arcturus Pico Pure Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide)
| | Sample_hyb_protocol | The NuGEN hybtidsation v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual
| | Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| | Sample_data_processing | The data were analyzed in R (version 2.8.1) using Affy package and RMA as the normalisation method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei,,Dai
| | Sample_contact_email | w.dai@imperial.ac.uk
| | Sample_contact_laboratory | Epigenetics
| | Sample_contact_department | Sugery and Cancer
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618724/suppl/GSM618724_1207_SK_3_7_H_IGSP2.CEL.gz
| | Sample_series_id | GSE25191
| | Sample_data_row_count | 54675
| | |
|
GSM618725 | GPL570 |
|
SP cell, biological replicate3
|
IGROV1, SP cell
|
cell type: side population cell
cell line source: IGROV1
|
Gene expression data from IGROV1 SP cell
|
| Sample_geo_accession | GSM618725
| | Sample_status | Public on Feb 09 2011
| | Sample_submission_date | Nov 08 2010
| | Sample_last_update_date | Feb 10 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Hoechst 33342 cell sorting
| | Sample_growth_protocol_ch1 | IGROV1 cell line was maintained in RPMI 1640 (Invitrogen, Paisley, UK) containing 10% fetal bovine serum (PAA Laboratories, Yeovil, UK) in humidified 5% CO2 in air incubators at 37 ̊C
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells isolated by FACS and RNA isolated using Arcturus Pico Pure Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The RNA was amplified using the WT-Ovation Pico target pre v 1.0 protocol which was derived from the NuGEN WT-Ovation Pico RNA Amplication system (WTO_Pico_UserGuide) and the NuGEN FL-Ovation cNDA Biotin Module v2 (FLBv2_User_Guide)
| | Sample_hyb_protocol | The NuGEN hybtidsation v1.0 protocol was followed for hybridising labelled DNA to standard GeneChips with 11 micron feature size. This hybridisation protocol is derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide and the Affymetrix GeneChip Expression Analysis Technical Manual
| | Sample_scan_protocol | The Affymetrix GeneChip scanner 3000 running GCOS software
| | Sample_data_processing | The data were analyzed in R (version 2.8.1) using Affy package and RMA as the normalisation method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei,,Dai
| | Sample_contact_email | w.dai@imperial.ac.uk
| | Sample_contact_laboratory | Epigenetics
| | Sample_contact_department | Sugery and Cancer
| | Sample_contact_institute | Imperial College London
| | Sample_contact_address | Du Cane Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | W12 0NN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618725/suppl/GSM618725_1207_SK_3_8_H_IGSP3.CEL.gz
| | Sample_series_id | GSE25191
| | Sample_data_row_count | 54675
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