Search results for the GEO ID: GSE25252  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM621280 | GPL1261 |
|
Foxp3 transduced epigenetics(+) T cells, rep1
|
Foxp3 transduced epigenetics(+) T cells
|
strain/background: DEREG_Balb/c
tissue: spleen
cell type: T cell
|
01_D_S GFP+ Foxp3 1_No_1201_(Mouse430_2)
|
| Sample_geo_accession | GSM621280
| | Sample_status | Public on Nov 05 2012
| | Sample_submission_date | Nov 09 2010
| | Sample_last_update_date | Nov 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis. To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared using the FL-Ovation cDNA Biotin Module V2 (NuGEN) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cDNA were hybridized for 18 hr on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner3000 G7 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Command Console Software (Affymetrix) and CHP files were generated by Affymetrix Expression Console Software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hiromasa,,Morikawa
| | Sample_contact_email | hiromasa-morikawa@umin.ac.jp
| | Sample_contact_department | Department of Experimental Pathology
| | Sample_contact_institute | Institute for Frontier Medical Sciences, Kyoto University
| | Sample_contact_address | 53 Kawaharacho, Shougoin, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621280/suppl/GSM621280.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621280/suppl/GSM621280.CHP.gz
| | Sample_series_id | GSE25252
| | Sample_data_row_count | 45101
| | |
|
GSM621281 | GPL1261 |
|
Foxp3 transduced epigenetics(+) T cells, rep2
|
Foxp3 transduced epigenetics(+) T cells
|
strain/background: DEREG_Balb/c
tissue: spleen
cell type: T cell
|
02_D_S GFP+ Foxp3 2 No_1201-2_(Mouse430_2)
|
| Sample_geo_accession | GSM621281
| | Sample_status | Public on Nov 05 2012
| | Sample_submission_date | Nov 09 2010
| | Sample_last_update_date | Nov 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis. To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared using the FL-Ovation cDNA Biotin Module V2 (NuGEN) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cDNA were hybridized for 18 hr on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner3000 G7 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Command Console Software (Affymetrix) and CHP files were generated by Affymetrix Expression Console Software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hiromasa,,Morikawa
| | Sample_contact_email | hiromasa-morikawa@umin.ac.jp
| | Sample_contact_department | Department of Experimental Pathology
| | Sample_contact_institute | Institute for Frontier Medical Sciences, Kyoto University
| | Sample_contact_address | 53 Kawaharacho, Shougoin, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621281/suppl/GSM621281.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621281/suppl/GSM621281.CHP.gz
| | Sample_series_id | GSE25252
| | Sample_data_row_count | 45101
| | |
|
GSM621282 | GPL1261 |
|
Foxp3(-)epigenetics(+) T cells, rep1
|
Foxp3(-)epigenetics(+) T cells
|
strain/background: DEREG_Balb/c
tissue: spleen
cell type: T cell
|
03_D_S GFP+ Mock 1 No_1201_(Mouse430_2)
|
| Sample_geo_accession | GSM621282
| | Sample_status | Public on Nov 05 2012
| | Sample_submission_date | Nov 09 2010
| | Sample_last_update_date | Nov 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis. To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared using the FL-Ovation cDNA Biotin Module V2 (NuGEN) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cDNA were hybridized for 18 hr on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner3000 G7 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Command Console Software (Affymetrix) and CHP files were generated by Affymetrix Expression Console Software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hiromasa,,Morikawa
| | Sample_contact_email | hiromasa-morikawa@umin.ac.jp
| | Sample_contact_department | Department of Experimental Pathology
| | Sample_contact_institute | Institute for Frontier Medical Sciences, Kyoto University
| | Sample_contact_address | 53 Kawaharacho, Shougoin, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621282/suppl/GSM621282.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621282/suppl/GSM621282.CHP.gz
| | Sample_series_id | GSE25252
| | Sample_data_row_count | 45101
| | |
|
GSM621283 | GPL1261 |
|
Foxp3(-)epigenetics(+) T cells, rep2
|
Foxp3(-)epigenetics(+) T cells
|
strain/background: DEREG_Balb/c
tissue: spleen
cell type: T cell
|
04_D_S GFP+ Mock 2 No_1201_(Mouse430_2)
|
| Sample_geo_accession | GSM621283
| | Sample_status | Public on Nov 05 2012
| | Sample_submission_date | Nov 09 2010
| | Sample_last_update_date | Nov 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis. To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared using the FL-Ovation cDNA Biotin Module V2 (NuGEN) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cDNA were hybridized for 18 hr on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner3000 G7 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Command Console Software (Affymetrix) and CHP files were generated by Affymetrix Expression Console Software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hiromasa,,Morikawa
| | Sample_contact_email | hiromasa-morikawa@umin.ac.jp
| | Sample_contact_department | Department of Experimental Pathology
| | Sample_contact_institute | Institute for Frontier Medical Sciences, Kyoto University
| | Sample_contact_address | 53 Kawaharacho, Shougoin, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621283/suppl/GSM621283.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621283/suppl/GSM621283.CHP.gz
| | Sample_series_id | GSE25252
| | Sample_data_row_count | 45101
| | |
|
GSM621284 | GPL1261 |
|
Foxp3(+)epigenetics(+) T cells, rep1
|
Foxp3(+)epigenetics(+) T cells
|
strain/background: DEREG_Balb/c
tissue: spleen
cell type: T cell
|
05_DEREG GFP+ Mock 1 No_1201_(Mouse430_2)
|
| Sample_geo_accession | GSM621284
| | Sample_status | Public on Nov 05 2012
| | Sample_submission_date | Nov 09 2010
| | Sample_last_update_date | Nov 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis. To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared using the FL-Ovation cDNA Biotin Module V2 (NuGEN) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cDNA were hybridized for 18 hr on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner3000 G7 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Command Console Software (Affymetrix) and CHP files were generated by Affymetrix Expression Console Software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hiromasa,,Morikawa
| | Sample_contact_email | hiromasa-morikawa@umin.ac.jp
| | Sample_contact_department | Department of Experimental Pathology
| | Sample_contact_institute | Institute for Frontier Medical Sciences, Kyoto University
| | Sample_contact_address | 53 Kawaharacho, Shougoin, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621284/suppl/GSM621284.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621284/suppl/GSM621284.CHP.gz
| | Sample_series_id | GSE25252
| | Sample_data_row_count | 45101
| | |
|
GSM621285 | GPL1261 |
|
Foxp3(+)epigenetics(+) T cells, rep2
|
Foxp3(+)epigenetics(+) T cells
|
strain/background: DEREG_Balb/c
tissue: spleen
cell type: T cell
|
06_DEREG GFP+ Mock 2_No_1201_(Mouse430_2)
|
| Sample_geo_accession | GSM621285
| | Sample_status | Public on Nov 05 2012
| | Sample_submission_date | Nov 09 2010
| | Sample_last_update_date | Nov 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis. To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared using the FL-Ovation cDNA Biotin Module V2 (NuGEN) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cDNA were hybridized for 18 hr on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner3000 G7 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Command Console Software (Affymetrix) and CHP files were generated by Affymetrix Expression Console Software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hiromasa,,Morikawa
| | Sample_contact_email | hiromasa-morikawa@umin.ac.jp
| | Sample_contact_department | Department of Experimental Pathology
| | Sample_contact_institute | Institute for Frontier Medical Sciences, Kyoto University
| | Sample_contact_address | 53 Kawaharacho, Shougoin, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621285/suppl/GSM621285.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621285/suppl/GSM621285.CHP.gz
| | Sample_series_id | GSE25252
| | Sample_data_row_count | 45101
| | |
|
GSM621286 | GPL1261 |
|
Foxp3 transduced epigenetics(-) T cells, rep1
|
Foxp3 transduced epigenetics(-) T cells
|
strain/background: DEREG_Balb/c
tissue: spleen
cell type: T cell
|
07_DEREG GFP- Foxp3 1_No_1201_(Mouse430_2)
|
| Sample_geo_accession | GSM621286
| | Sample_status | Public on Nov 05 2012
| | Sample_submission_date | Nov 09 2010
| | Sample_last_update_date | Nov 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis. To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared using the FL-Ovation cDNA Biotin Module V2 (NuGEN) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cDNA were hybridized for 18 hr on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner3000 G7 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Command Console Software (Affymetrix) and CHP files were generated by Affymetrix Expression Console Software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hiromasa,,Morikawa
| | Sample_contact_email | hiromasa-morikawa@umin.ac.jp
| | Sample_contact_department | Department of Experimental Pathology
| | Sample_contact_institute | Institute for Frontier Medical Sciences, Kyoto University
| | Sample_contact_address | 53 Kawaharacho, Shougoin, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621286/suppl/GSM621286.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621286/suppl/GSM621286.CHP.gz
| | Sample_series_id | GSE25252
| | Sample_data_row_count | 45101
| | |
|
GSM621287 | GPL1261 |
|
Foxp3 transduced epigenetics(-) T cells, rep2
|
Foxp3 transduced epigenetics(-) T cells
|
strain/background: DEREG_Balb/c
tissue: spleen
cell type: T cell
|
08_DEREG GFP- Foxp3 2_No_1201_(Mouse430_2)
|
| Sample_geo_accession | GSM621287
| | Sample_status | Public on Nov 05 2012
| | Sample_submission_date | Nov 09 2010
| | Sample_last_update_date | Nov 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis. To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared using the FL-Ovation cDNA Biotin Module V2 (NuGEN) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cDNA were hybridized for 18 hr on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner3000 G7 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Command Console Software (Affymetrix) and CHP files were generated by Affymetrix Expression Console Software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hiromasa,,Morikawa
| | Sample_contact_email | hiromasa-morikawa@umin.ac.jp
| | Sample_contact_department | Department of Experimental Pathology
| | Sample_contact_institute | Institute for Frontier Medical Sciences, Kyoto University
| | Sample_contact_address | 53 Kawaharacho, Shougoin, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621287/suppl/GSM621287.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621287/suppl/GSM621287.CHP.gz
| | Sample_series_id | GSE25252
| | Sample_data_row_count | 45101
| | |
|
GSM621288 | GPL1261 |
|
Foxp3(-)epigenetics(-) T cells, rep1
|
Foxp3(-)epigenetics(-) T cells
|
strain/background: DEREG_Balb/c
tissue: spleen
cell type: T cell
|
09_DEREG GFP- Mock 1_No_1201_(Mouse430_2)
|
| Sample_geo_accession | GSM621288
| | Sample_status | Public on Nov 05 2012
| | Sample_submission_date | Nov 09 2010
| | Sample_last_update_date | Nov 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis. To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared using the FL-Ovation cDNA Biotin Module V2 (NuGEN) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cDNA were hybridized for 18 hr on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner3000 G7 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Command Console Software (Affymetrix) and CHP files were generated by Affymetrix Expression Console Software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hiromasa,,Morikawa
| | Sample_contact_email | hiromasa-morikawa@umin.ac.jp
| | Sample_contact_department | Department of Experimental Pathology
| | Sample_contact_institute | Institute for Frontier Medical Sciences, Kyoto University
| | Sample_contact_address | 53 Kawaharacho, Shougoin, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621288/suppl/GSM621288.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621288/suppl/GSM621288.CHP.gz
| | Sample_series_id | GSE25252
| | Sample_data_row_count | 45101
| | |
|
GSM621289 | GPL1261 |
|
Foxp3(-)epigenetics(-) T cells, rep2
|
Foxp3(-)epigenetics(-) T cells
|
strain/background: DEREG_Balb/c
tissue: spleen
cell type: T cell
|
10_DEREG GFP- Mock 2_No_1201_(Mouse430_2)
|
| Sample_geo_accession | GSM621289
| | Sample_status | Public on Nov 05 2012
| | Sample_submission_date | Nov 09 2010
| | Sample_last_update_date | Nov 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis. To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cDNA were prepared using the FL-Ovation cDNA Biotin Module V2 (NuGEN) according to the manufacturer's instructions.
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cDNA were hybridized for 18 hr on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner3000 G7 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Command Console Software (Affymetrix) and CHP files were generated by Affymetrix Expression Console Software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hiromasa,,Morikawa
| | Sample_contact_email | hiromasa-morikawa@umin.ac.jp
| | Sample_contact_department | Department of Experimental Pathology
| | Sample_contact_institute | Institute for Frontier Medical Sciences, Kyoto University
| | Sample_contact_address | 53 Kawaharacho, Shougoin, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_state | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621289/suppl/GSM621289.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM621nnn/GSM621289/suppl/GSM621289.CHP.gz
| | Sample_series_id | GSE25252
| | Sample_data_row_count | 45101
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