Search results for the GEO ID: GSE25417 |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM624086 | GPL570 |
|
H9_d5_hepatic differentiated_1
|
WA09 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells
growth protocol: subjected to a hepatic differentiation protocol
time: d5
|
The availability of pluripotent stem cells offers the possibility of using such cells to model hepatic disease and development. With this in mind, we previously established a protocol that facilitates the differentiation of both human embryonic stem cells and induced pluritpotent cells into cells with hepatocyte characteristics. The use of highly defined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and reproducible differentiation to occur. The differentiation toward a hepatocyte–like fate appeared to recapitulate many of the stages normally associated with the formation of hepatocytes in vivo. In the current study we addressed the feasibility of using human pluripotent stem cells to probe the molecular mechanisms underlying human hepatocyte differentiation. We demonstrate i) that human ES cells express a number of mRNAs that characterize each stage in the differentiation process, ii) that gene expression can be efficiently depleted throughout the differentiation time course using shRNAs expressed from lentiviruses, and iii) that the nuclear hormone receptor HNF4a is essential for specification of human hepatic progenitor cells by establishing expression of the network of transcription factors that control hepatocyte cell fate.
|
Sample_geo_accession | GSM624086
| Sample_status | Public on Aug 22 2011
| Sample_submission_date | Nov 16 2010
| Sample_last_update_date | Aug 22 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624086/suppl/GSM624086.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624086/suppl/GSM624086.CHP.gz
| Sample_series_id | GSE25417
| Sample_data_row_count | 54675
| |
|
GSM624087 | GPL570 |
|
H9_d5_hepatic differentiated_2
|
WAO9 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells
growth protocol: subjected to a hepatic differentiation protocol
time: d5
|
The availability of pluripotent stem cells offers the possibility of using such cells to model hepatic disease and development. With this in mind, we previously established a protocol that facilitates the differentiation of both human embryonic stem cells and induced pluritpotent cells into cells with hepatocyte characteristics. The use of highly defined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and reproducible differentiation to occur. The differentiation toward a hepatocyte–like fate appeared to recapitulate many of the stages normally associated with the formation of hepatocytes in vivo. In the current study we addressed the feasibility of using human pluripotent stem cells to probe the molecular mechanisms underlying human hepatocyte differentiation. We demonstrate i) that human ES cells express a number of mRNAs that characterize each stage in the differentiation process, ii) that gene expression can be efficiently depleted throughout the differentiation time course using shRNAs expressed from lentiviruses, and iii) that the nuclear hormone receptor HNF4a is essential for specification of human hepatic progenitor cells by establishing expression of the network of transcription factors that control hepatocyte cell fate.
|
Sample_geo_accession | GSM624087
| Sample_status | Public on Aug 22 2011
| Sample_submission_date | Nov 16 2010
| Sample_last_update_date | Aug 22 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624087/suppl/GSM624087.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624087/suppl/GSM624087.CHP.gz
| Sample_series_id | GSE25417
| Sample_data_row_count | 54675
| |
|
GSM624089 | GPL570 |
|
H9_d5_hepatic differentiated_3
|
WA09 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells
growth protocol: subjected to a hepatic differentiation protocol
time: d5
|
The availability of pluripotent stem cells offers the possibility of using such cells to model hepatic disease and development. With this in mind, we previously established a protocol that facilitates the differentiation of both human embryonic stem cells and induced pluritpotent cells into cells with hepatocyte characteristics. The use of highly defined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and reproducible differentiation to occur. The differentiation toward a hepatocyte–like fate appeared to recapitulate many of the stages normally associated with the formation of hepatocytes in vivo. In the current study we addressed the feasibility of using human pluripotent stem cells to probe the molecular mechanisms underlying human hepatocyte differentiation. We demonstrate i) that human ES cells express a number of mRNAs that characterize each stage in the differentiation process, ii) that gene expression can be efficiently depleted throughout the differentiation time course using shRNAs expressed from lentiviruses, and iii) that the nuclear hormone receptor HNF4a is essential for specification of human hepatic progenitor cells by establishing expression of the network of transcription factors that control hepatocyte cell fate.
|
Sample_geo_accession | GSM624089
| Sample_status | Public on Aug 22 2011
| Sample_submission_date | Nov 16 2010
| Sample_last_update_date | Aug 22 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624089/suppl/GSM624089.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624089/suppl/GSM624089.CHP.gz
| Sample_series_id | GSE25417
| Sample_data_row_count | 54675
| |
|
GSM624090 | GPL570 |
|
H9_d10_hepatic differentiated_1
|
WA09 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells
growth protocol: subjected to a hepatic differentiation protocol
time: d10
|
The availability of pluripotent stem cells offers the possibility of using such cells to model hepatic disease and development. With this in mind, we previously established a protocol that facilitates the differentiation of both human embryonic stem cells and induced pluritpotent cells into cells with hepatocyte characteristics. The use of highly defined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and reproducible differentiation to occur. The differentiation toward a hepatocyte–like fate appeared to recapitulate many of the stages normally associated with the formation of hepatocytes in vivo. In the current study we addressed the feasibility of using human pluripotent stem cells to probe the molecular mechanisms underlying human hepatocyte differentiation. We demonstrate i) that human ES cells express a number of mRNAs that characterize each stage in the differentiation process, ii) that gene expression can be efficiently depleted throughout the differentiation time course using shRNAs expressed from lentiviruses, and iii) that the nuclear hormone receptor HNF4a is essential for specification of human hepatic progenitor cells by establishing expression of the network of transcription factors that control hepatocyte cell fate.
|
Sample_geo_accession | GSM624090
| Sample_status | Public on Aug 22 2011
| Sample_submission_date | Nov 16 2010
| Sample_last_update_date | Aug 22 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624090/suppl/GSM624090.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624090/suppl/GSM624090.CHP.gz
| Sample_series_id | GSE25417
| Sample_data_row_count | 54675
| |
|
GSM624091 | GPL570 |
|
H9_d10_hepatic differentiated_2
|
WA09 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells
growth protocol: subjected to a hepatic differentiation protocol
time: d10
|
The availability of pluripotent stem cells offers the possibility of using such cells to model hepatic disease and development. With this in mind, we previously established a protocol that facilitates the differentiation of both human embryonic stem cells and induced pluritpotent cells into cells with hepatocyte characteristics. The use of highly defined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and reproducible differentiation to occur. The differentiation toward a hepatocyte–like fate appeared to recapitulate many of the stages normally associated with the formation of hepatocytes in vivo. In the current study we addressed the feasibility of using human pluripotent stem cells to probe the molecular mechanisms underlying human hepatocyte differentiation. We demonstrate i) that human ES cells express a number of mRNAs that characterize each stage in the differentiation process, ii) that gene expression can be efficiently depleted throughout the differentiation time course using shRNAs expressed from lentiviruses, and iii) that the nuclear hormone receptor HNF4a is essential for specification of human hepatic progenitor cells by establishing expression of the network of transcription factors that control hepatocyte cell fate.
|
Sample_geo_accession | GSM624091
| Sample_status | Public on Aug 22 2011
| Sample_submission_date | Nov 16 2010
| Sample_last_update_date | Aug 22 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624091/suppl/GSM624091.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624091/suppl/GSM624091.CHP.gz
| Sample_series_id | GSE25417
| Sample_data_row_count | 54675
| |
|
GSM624092 | GPL570 |
|
H9_d10_hepatic differentiated_3
|
WA09 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells
growth protocol: subjected to a hepatic differentiation protocol
time: d10
|
The availability of pluripotent stem cells offers the possibility of using such cells to model hepatic disease and development. With this in mind, we previously established a protocol that facilitates the differentiation of both human embryonic stem cells and induced pluritpotent cells into cells with hepatocyte characteristics. The use of highly defined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and reproducible differentiation to occur. The differentiation toward a hepatocyte–like fate appeared to recapitulate many of the stages normally associated with the formation of hepatocytes in vivo. In the current study we addressed the feasibility of using human pluripotent stem cells to probe the molecular mechanisms underlying human hepatocyte differentiation. We demonstrate i) that human ES cells express a number of mRNAs that characterize each stage in the differentiation process, ii) that gene expression can be efficiently depleted throughout the differentiation time course using shRNAs expressed from lentiviruses, and iii) that the nuclear hormone receptor HNF4a is essential for specification of human hepatic progenitor cells by establishing expression of the network of transcription factors that control hepatocyte cell fate.
|
Sample_geo_accession | GSM624092
| Sample_status | Public on Aug 22 2011
| Sample_submission_date | Nov 16 2010
| Sample_last_update_date | Aug 22 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624092/suppl/GSM624092.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624092/suppl/GSM624092.CHP.gz
| Sample_series_id | GSE25417
| Sample_data_row_count | 54675
| |
|
GSM624094 | GPL570 |
|
H9_d15_hepatic differentiated_1
|
WA09 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells
growth protocol: subjected to a hepatic differentiation protocol
time: d15
|
The availability of pluripotent stem cells offers the possibility of using such cells to model hepatic disease and development. With this in mind, we previously established a protocol that facilitates the differentiation of both human embryonic stem cells and induced pluritpotent cells into cells with hepatocyte characteristics. The use of highly defined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and reproducible differentiation to occur. The differentiation toward a hepatocyte–like fate appeared to recapitulate many of the stages normally associated with the formation of hepatocytes in vivo. In the current study we addressed the feasibility of using human pluripotent stem cells to probe the molecular mechanisms underlying human hepatocyte differentiation. We demonstrate i) that human ES cells express a number of mRNAs that characterize each stage in the differentiation process, ii) that gene expression can be efficiently depleted throughout the differentiation time course using shRNAs expressed from lentiviruses, and iii) that the nuclear hormone receptor HNF4a is essential for specification of human hepatic progenitor cells by establishing expression of the network of transcription factors that control hepatocyte cell fate.
|
Sample_geo_accession | GSM624094
| Sample_status | Public on Aug 22 2011
| Sample_submission_date | Nov 16 2010
| Sample_last_update_date | Aug 22 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624094/suppl/GSM624094.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624094/suppl/GSM624094.CHP.gz
| Sample_series_id | GSE25417
| Sample_data_row_count | 54675
| |
|
GSM624095 | GPL570 |
|
H9_d15_hepatic differentiated_2
|
WA09 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells
growth protocol: subjected to a hepatic differentiation protocol
time: d15
|
The availability of pluripotent stem cells offers the possibility of using such cells to model hepatic disease and development. With this in mind, we previously established a protocol that facilitates the differentiation of both human embryonic stem cells and induced pluritpotent cells into cells with hepatocyte characteristics. The use of highly defined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and reproducible differentiation to occur. The differentiation toward a hepatocyte–like fate appeared to recapitulate many of the stages normally associated with the formation of hepatocytes in vivo. In the current study we addressed the feasibility of using human pluripotent stem cells to probe the molecular mechanisms underlying human hepatocyte differentiation. We demonstrate i) that human ES cells express a number of mRNAs that characterize each stage in the differentiation process, ii) that gene expression can be efficiently depleted throughout the differentiation time course using shRNAs expressed from lentiviruses, and iii) that the nuclear hormone receptor HNF4a is essential for specification of human hepatic progenitor cells by establishing expression of the network of transcription factors that control hepatocyte cell fate.
|
Sample_geo_accession | GSM624095
| Sample_status | Public on Aug 22 2011
| Sample_submission_date | Nov 16 2010
| Sample_last_update_date | Aug 22 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624095/suppl/GSM624095.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624095/suppl/GSM624095.CHP.gz
| Sample_series_id | GSE25417
| Sample_data_row_count | 54675
| |
|
GSM624096 | GPL570 |
|
H9_d15_hepatic differentiated_3
|
WA09 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells
growth protocol: subjected to a hepatic differentiation protocol
time: d15
|
The availability of pluripotent stem cells offers the possibility of using such cells to model hepatic disease and development. With this in mind, we previously established a protocol that facilitates the differentiation of both human embryonic stem cells and induced pluritpotent cells into cells with hepatocyte characteristics. The use of highly defined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and reproducible differentiation to occur. The differentiation toward a hepatocyte–like fate appeared to recapitulate many of the stages normally associated with the formation of hepatocytes in vivo. In the current study we addressed the feasibility of using human pluripotent stem cells to probe the molecular mechanisms underlying human hepatocyte differentiation. We demonstrate i) that human ES cells express a number of mRNAs that characterize each stage in the differentiation process, ii) that gene expression can be efficiently depleted throughout the differentiation time course using shRNAs expressed from lentiviruses, and iii) that the nuclear hormone receptor HNF4a is essential for specification of human hepatic progenitor cells by establishing expression of the network of transcription factors that control hepatocyte cell fate.
|
Sample_geo_accession | GSM624096
| Sample_status | Public on Aug 22 2011
| Sample_submission_date | Nov 16 2010
| Sample_last_update_date | Aug 22 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624096/suppl/GSM624096.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624096/suppl/GSM624096.CHP.gz
| Sample_series_id | GSE25417
| Sample_data_row_count | 54675
| |
|
GSM624098 | GPL570 |
|
hnf4KD_H9_d20_hepatic differentiated_1
|
WA09 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells
genotype/variation: shRNA-mediated HNF4a knockdown
growth protocol: subjected to a hepatic differentiation protocol
time: d20
|
The availability of pluripotent stem cells offers the possibility of using such cells to model hepatic disease and development. With this in mind, we previously established a protocol that facilitates the differentiation of both human embryonic stem cells and induced pluritpotent cells into cells with hepatocyte characteristics. The use of highly defined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and reproducible differentiation to occur. The differentiation toward a hepatocyte–like fate appeared to recapitulate many of the stages normally associated with the formation of hepatocytes in vivo. In the current study we addressed the feasibility of using human pluripotent stem cells to probe the molecular mechanisms underlying human hepatocyte differentiation. We demonstrate i) that human ES cells express a number of mRNAs that characterize each stage in the differentiation process, ii) that gene expression can be efficiently depleted throughout the differentiation time course using shRNAs expressed from lentiviruses, and iii) that the nuclear hormone receptor HNF4a is essential for specification of human hepatic progenitor cells by establishing expression of the network of transcription factors that control hepatocyte cell fate.
|
Sample_geo_accession | GSM624098
| Sample_status | Public on Aug 22 2011
| Sample_submission_date | Nov 16 2010
| Sample_last_update_date | Aug 22 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624098/suppl/GSM624098.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624098/suppl/GSM624098.CHP.gz
| Sample_series_id | GSE25417
| Sample_data_row_count | 54675
| |
|
GSM624099 | GPL570 |
|
hnf4KD_H9_d20_hepatic differentiated_2
|
WA09 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells
genotype/variation: shRNA-mediated HNF4a knockdown
growth protocol: subjected to a hepatic differentiation protocol
time: d20
|
The availability of pluripotent stem cells offers the possibility of using such cells to model hepatic disease and development. With this in mind, we previously established a protocol that facilitates the differentiation of both human embryonic stem cells and induced pluritpotent cells into cells with hepatocyte characteristics. The use of highly defined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and reproducible differentiation to occur. The differentiation toward a hepatocyte–like fate appeared to recapitulate many of the stages normally associated with the formation of hepatocytes in vivo. In the current study we addressed the feasibility of using human pluripotent stem cells to probe the molecular mechanisms underlying human hepatocyte differentiation. We demonstrate i) that human ES cells express a number of mRNAs that characterize each stage in the differentiation process, ii) that gene expression can be efficiently depleted throughout the differentiation time course using shRNAs expressed from lentiviruses, and iii) that the nuclear hormone receptor HNF4a is essential for specification of human hepatic progenitor cells by establishing expression of the network of transcription factors that control hepatocyte cell fate.
|
Sample_geo_accession | GSM624099
| Sample_status | Public on Aug 22 2011
| Sample_submission_date | Nov 16 2010
| Sample_last_update_date | Aug 22 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624099/suppl/GSM624099.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624099/suppl/GSM624099.CHP.gz
| Sample_series_id | GSE25417
| Sample_data_row_count | 54675
| |
|
GSM624100 | GPL570 |
|
hnf4KD_H9_d20_hepatic differentiated_3
|
WA09 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells
genotype/variation: shRNA-mediated HNF4a knockdown
growth protocol: subjected to a hepatic differentiation protocol
time: d20
|
The availability of pluripotent stem cells offers the possibility of using such cells to model hepatic disease and development. With this in mind, we previously established a protocol that facilitates the differentiation of both human embryonic stem cells and induced pluritpotent cells into cells with hepatocyte characteristics. The use of highly defined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and reproducible differentiation to occur. The differentiation toward a hepatocyte–like fate appeared to recapitulate many of the stages normally associated with the formation of hepatocytes in vivo. In the current study we addressed the feasibility of using human pluripotent stem cells to probe the molecular mechanisms underlying human hepatocyte differentiation. We demonstrate i) that human ES cells express a number of mRNAs that characterize each stage in the differentiation process, ii) that gene expression can be efficiently depleted throughout the differentiation time course using shRNAs expressed from lentiviruses, and iii) that the nuclear hormone receptor HNF4a is essential for specification of human hepatic progenitor cells by establishing expression of the network of transcription factors that control hepatocyte cell fate.
|
Sample_geo_accession | GSM624100
| Sample_status | Public on Aug 22 2011
| Sample_submission_date | Nov 16 2010
| Sample_last_update_date | Aug 22 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624100/suppl/GSM624100.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624100/suppl/GSM624100.CHP.gz
| Sample_series_id | GSE25417
| Sample_data_row_count | 54675
| |
|
|
|
Make groups for comparisons |
(2 groups will be compared at a time) |
|
Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|