Search results for the GEO ID: GSE25438 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM624380 | GPL1261 |
|
Control Epithelium-6 hr
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Nasal Epithelium from CD-1 Mice 6 hours following PBS exposure
|
gender: female
tissue: nasal epithelium
strain: CD-1
age: 8 weeks
agent: PBS
time point: 6 hours
|
Gene expression data 6 hours following exposure to PBS
|
Sample_geo_accession | GSM624380
| Sample_status | Public on Nov 18 2010
| Sample_submission_date | Nov 17 2010
| Sample_last_update_date | Nov 17 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | 5 microliters per nare of either sterile PBS (control) or 20% v/v NE (W805EC) was administered via pipette. Nasal epithelium was harvested immediatley post-mortem either 6 or 24 hours and cryopreseved in OTC via immersion in liquid nitrogen.
| Sample_growth_protocol_ch1 | Mice were maintained in standard conditions for 6 or 24 hours following nasal adminsistration of either PBS or NE.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted per sample using RNA easy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit rotocol from 50 ng total RNA (Ovation RN Amplfication System V2 Manual, 2007, NuGen)
| Sample_hyb_protocol | Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 Array. GeneChips were washed and stained in the Affymetrix Fluidics station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-Statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Paul,Edward,Makidon
| Sample_contact_email | pmakidon@gmail.com
| Sample_contact_phone | 734-277-5775
| Sample_contact_fax | 734-936-2990
| Sample_contact_laboratory | MNIMBS
| Sample_contact_department | Internal Medicine
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 9340 MSRB III, 1150 W. Medical Center Dr.
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624380/suppl/GSM624380.CEL.gz
| Sample_series_id | GSE25438
| Sample_series_id | GSE25486
| Sample_data_row_count | 45101
| |
|
GSM624381 | GPL1261 |
|
NE Treated Epithelium_6 hr
|
Nasal Epithelium from CD-1 Mice 6 hours following 20% NE exposure
|
gender: female
tissue: nasal epithelium
strain: CD-1
age: 8 weeks
agent: 20% (v/v) naomeulsion (W805EC)
time point: 6 hours
|
Gene expression data 6 hours following exposure to NE
|
Sample_geo_accession | GSM624381
| Sample_status | Public on Nov 18 2010
| Sample_submission_date | Nov 17 2010
| Sample_last_update_date | Nov 17 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | 5 microliters per nare of either sterile PBS (control) or 20% v/v NE (W805EC) was administered via pipette. Nasal epithelium was harvested immediatley post-mortem either 6 or 24 hours and cryopreseved in OTC via immersion in liquid nitrogen.
| Sample_growth_protocol_ch1 | Mice were maintained in standard conditions for 6 or 24 hours following nasal adminsistration of either PBS or NE.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted per sample using RNA easy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit rotocol from 50 ng total RNA (Ovation RN Amplfication System V2 Manual, 2007, NuGen)
| Sample_hyb_protocol | Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 Array. GeneChips were washed and stained in the Affymetrix Fluidics station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-Statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Paul,Edward,Makidon
| Sample_contact_email | pmakidon@gmail.com
| Sample_contact_phone | 734-277-5775
| Sample_contact_fax | 734-936-2990
| Sample_contact_laboratory | MNIMBS
| Sample_contact_department | Internal Medicine
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 9340 MSRB III, 1150 W. Medical Center Dr.
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624381/suppl/GSM624381.CEL.gz
| Sample_series_id | GSE25438
| Sample_series_id | GSE25486
| Sample_data_row_count | 45101
| |
|
GSM624382 | GPL1261 |
|
Control Epithelium_24 hr
|
Nasal Epithelium from CD-1 Mice 24 hours following PBS exposure
|
gender: female
tissue: nasal epithelium
strain: CD-1
age: 8 weeks
agent: PBS
time point: 24 hours
|
Gene expression data 24 hours following exposure to PBS
|
Sample_geo_accession | GSM624382
| Sample_status | Public on Nov 18 2010
| Sample_submission_date | Nov 17 2010
| Sample_last_update_date | Nov 17 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | 5 microliters per nare of either sterile PBS (control) or 20% v/v NE (W805EC) was administered via pipette. Nasal epithelium was harvested immediatley post-mortem either 6 or 24 hours and cryopreseved in OTC via immersion in liquid nitrogen.
| Sample_growth_protocol_ch1 | Mice were maintained in standard conditions for 6 or 24 hours following nasal adminsistration of either PBS or NE.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted per sample using RNA easy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit rotocol from 50 ng total RNA (Ovation RN Amplfication System V2 Manual, 2007, NuGen)
| Sample_hyb_protocol | Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 Array. GeneChips were washed and stained in the Affymetrix Fluidics station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-Statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Paul,Edward,Makidon
| Sample_contact_email | pmakidon@gmail.com
| Sample_contact_phone | 734-277-5775
| Sample_contact_fax | 734-936-2990
| Sample_contact_laboratory | MNIMBS
| Sample_contact_department | Internal Medicine
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 9340 MSRB III, 1150 W. Medical Center Dr.
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624382/suppl/GSM624382.CEL.gz
| Sample_series_id | GSE25438
| Sample_series_id | GSE25486
| Sample_data_row_count | 45101
| |
|
GSM624383 | GPL1261 |
|
NE Treated Epithelium_24 hr
|
Nasal Epithelium from CD-1 Mice 24 hours following 20% NE exposure
|
gender: female
tissue: nasal epithelium
strain: CD-1
age: 8 weeks
agent: 20% (v/v) naomeulsion (W805EC)
time point: 24 hours
|
Gene expression data 24 hours following exposure to NE
|
Sample_geo_accession | GSM624383
| Sample_status | Public on Nov 18 2010
| Sample_submission_date | Nov 17 2010
| Sample_last_update_date | Nov 17 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | 5 microliters per nare of either sterile PBS (control) or 20% v/v NE (W805EC) was administered via pipette. Nasal epithelium was harvested immediatley post-mortem either 6 or 24 hours and cryopreseved in OTC via immersion in liquid nitrogen.
| Sample_growth_protocol_ch1 | Mice were maintained in standard conditions for 6 or 24 hours following nasal adminsistration of either PBS or NE.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted per sample using RNA easy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit rotocol from 50 ng total RNA (Ovation RN Amplfication System V2 Manual, 2007, NuGen)
| Sample_hyb_protocol | Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 Array. GeneChips were washed and stained in the Affymetrix Fluidics station 450
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-Statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Paul,Edward,Makidon
| Sample_contact_email | pmakidon@gmail.com
| Sample_contact_phone | 734-277-5775
| Sample_contact_fax | 734-936-2990
| Sample_contact_laboratory | MNIMBS
| Sample_contact_department | Internal Medicine
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 9340 MSRB III, 1150 W. Medical Center Dr.
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624383/suppl/GSM624383.CEL.gz
| Sample_series_id | GSE25438
| Sample_series_id | GSE25486
| Sample_data_row_count | 45101
| |
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