Search results for the GEO ID: GSE25458  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM624841 | GPL570 |
|
Ishikawa treated with metastin-10, biological rep1
|
Ishikawa cells treated with metastin-10
|
cell line: Ishikawa
cell type: endometrial cancer cells
sirna transfection: negative control siRNA
peptide treatment: metastin-10
|
Gene expression data from Ishikawa cells treated with metastin-10.
|
| Sample_geo_accession | GSM624841
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Nov 17 2010
| | Sample_last_update_date | Nov 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At ~70% confluence, cells were transfected with negative control siRNA or GPR54-specific siRNA for 48 hrs, and then treated with either 10 µM metastin-10 or control peptide for the following 24 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 media with 10% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until ~70% confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix one cycle IVT.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Data were RMA normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tsukasa,,Baba
| | Sample_contact_email | babatsu@kuhp.kyoto-u.ac.jp
| | Sample_contact_department | Department of Gynecology and Obstetrics
| | Sample_contact_institute | Kyoto University Graduate School of Medicine
| | Sample_contact_address | 54 Shogoin Kawahara-cho, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624841/suppl/GSM624841.CEL.gz
| | Sample_series_id | GSE25458
| | Sample_data_row_count | 54675
| | |
|
GSM624842 | GPL570 |
|
Ishikawa treated with metastin-10, biological rep2
|
Ishikawa cells treated with metastin-10
|
cell line: Ishikawa
cell type: endometrial cancer cells
sirna transfection: negative control siRNA
peptide treatment: metastin-10
|
Gene expression data from Ishikawa cells treated with metastin-10.
|
| Sample_geo_accession | GSM624842
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Nov 17 2010
| | Sample_last_update_date | Nov 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At ~70% confluence, cells were transfected with negative control siRNA or GPR54-specific siRNA for 48 hrs, and then treated with either 10 µM metastin-10 or control peptide for the following 24 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 media with 10% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until ~70% confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix one cycle IVT.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Data were RMA normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tsukasa,,Baba
| | Sample_contact_email | babatsu@kuhp.kyoto-u.ac.jp
| | Sample_contact_department | Department of Gynecology and Obstetrics
| | Sample_contact_institute | Kyoto University Graduate School of Medicine
| | Sample_contact_address | 54 Shogoin Kawahara-cho, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624842/suppl/GSM624842.CEL.gz
| | Sample_series_id | GSE25458
| | Sample_data_row_count | 54675
| | |
|
GSM624843 | GPL570 |
|
Ishikawa treated with metastin-10, biological rep3
|
Ishikawa cells treated with metastin-10
|
cell line: Ishikawa
cell type: endometrial cancer cells
sirna transfection: negative control siRNA
peptide treatment: metastin-10
|
Gene expression data from Ishikawa cells treated with metastin-10.
|
| Sample_geo_accession | GSM624843
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Nov 17 2010
| | Sample_last_update_date | Nov 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At ~70% confluence, cells were transfected with negative control siRNA or GPR54-specific siRNA for 48 hrs, and then treated with either 10 µM metastin-10 or control peptide for the following 24 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 media with 10% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until ~70% confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix one cycle IVT.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Data were RMA normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tsukasa,,Baba
| | Sample_contact_email | babatsu@kuhp.kyoto-u.ac.jp
| | Sample_contact_department | Department of Gynecology and Obstetrics
| | Sample_contact_institute | Kyoto University Graduate School of Medicine
| | Sample_contact_address | 54 Shogoin Kawahara-cho, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624843/suppl/GSM624843.CEL.gz
| | Sample_series_id | GSE25458
| | Sample_data_row_count | 54675
| | |
|
GSM624844 | GPL570 |
|
Ishikawa non-treated, biological rep1
|
Ishikawa cells not introduced with GPR54 siRNA and not treated with metastin-10
|
cell line: Ishikawa
cell type: endometrial cancer cells
sirna transfection: negative control siRNA
peptide treatment: control peptide
|
Gene expression data from Ishikawa cells that were not treated with metastin-10 and not introduced with GPR54 siRNA.
|
| Sample_geo_accession | GSM624844
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Nov 17 2010
| | Sample_last_update_date | Nov 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At ~70% confluence, cells were transfected with negative control siRNA or GPR54-specific siRNA for 48 hrs, and then treated with either 10 µM metastin-10 or control peptide for the following 24 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 media with 10% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until ~70% confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix one cycle IVT.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Data were RMA normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tsukasa,,Baba
| | Sample_contact_email | babatsu@kuhp.kyoto-u.ac.jp
| | Sample_contact_department | Department of Gynecology and Obstetrics
| | Sample_contact_institute | Kyoto University Graduate School of Medicine
| | Sample_contact_address | 54 Shogoin Kawahara-cho, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624844/suppl/GSM624844.CEL.gz
| | Sample_series_id | GSE25458
| | Sample_data_row_count | 54675
| | |
|
GSM624845 | GPL570 |
|
Ishikawa non-treated, biological rep2
|
Ishikawa cells not introduced with GPR54 siRNA and not treated with metastin-10
|
cell line: Ishikawa
cell type: endometrial cancer cells
sirna transfection: negative control siRNA
peptide treatment: control peptide
|
Gene expression data from Ishikawa cells that were not treated with metastin-10 and not introduced with GPR54 siRNA.
|
| Sample_geo_accession | GSM624845
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Nov 17 2010
| | Sample_last_update_date | Nov 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At ~70% confluence, cells were transfected with negative control siRNA or GPR54-specific siRNA for 48 hrs, and then treated with either 10 µM metastin-10 or control peptide for the following 24 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 media with 10% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until ~70% confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix one cycle IVT.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Data were RMA normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tsukasa,,Baba
| | Sample_contact_email | babatsu@kuhp.kyoto-u.ac.jp
| | Sample_contact_department | Department of Gynecology and Obstetrics
| | Sample_contact_institute | Kyoto University Graduate School of Medicine
| | Sample_contact_address | 54 Shogoin Kawahara-cho, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624845/suppl/GSM624845.CEL.gz
| | Sample_series_id | GSE25458
| | Sample_data_row_count | 54675
| | |
|
GSM624846 | GPL570 |
|
Ishikawa non-treated, biological rep3
|
Ishikawa cells not introduced with GPR54 siRNA and not treated with metastin-10
|
cell line: Ishikawa
cell type: endometrial cancer cells
sirna transfection: negative control siRNA
peptide treatment: control peptide
|
Gene expression data from Ishikawa cells that were not treated with metastin-10 and not introduced with GPR54 siRNA.
|
| Sample_geo_accession | GSM624846
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Nov 17 2010
| | Sample_last_update_date | Nov 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At ~70% confluence, cells were transfected with negative control siRNA or GPR54-specific siRNA for 48 hrs, and then treated with either 10 µM metastin-10 or control peptide for the following 24 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 media with 10% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until ~70% confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix one cycle IVT.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Data were RMA normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tsukasa,,Baba
| | Sample_contact_email | babatsu@kuhp.kyoto-u.ac.jp
| | Sample_contact_department | Department of Gynecology and Obstetrics
| | Sample_contact_institute | Kyoto University Graduate School of Medicine
| | Sample_contact_address | 54 Shogoin Kawahara-cho, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624846/suppl/GSM624846.CEL.gz
| | Sample_series_id | GSE25458
| | Sample_data_row_count | 54675
| | |
|
GSM624847 | GPL570 |
|
GPR54-suppressed Ishikawa, biological rep1
|
Ishikawa cells introduced with GPR54 siRNA
|
cell line: Ishikawa
cell type: endometrial cancer cells
sirna transfection: GPR54-specific siRNA
peptide treatment: control peptide
|
Gene expression data from Ishikawa cells that were introduced with GPR54 siRNA.
|
| Sample_geo_accession | GSM624847
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Nov 17 2010
| | Sample_last_update_date | Nov 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At ~70% confluence, cells were transfected with negative control siRNA or GPR54-specific siRNA for 48 hrs, and then treated with either 10 µM metastin-10 or control peptide for the following 24 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 media with 10% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until ~70% confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix one cycle IVT.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Data were RMA normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tsukasa,,Baba
| | Sample_contact_email | babatsu@kuhp.kyoto-u.ac.jp
| | Sample_contact_department | Department of Gynecology and Obstetrics
| | Sample_contact_institute | Kyoto University Graduate School of Medicine
| | Sample_contact_address | 54 Shogoin Kawahara-cho, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624847/suppl/GSM624847.CEL.gz
| | Sample_series_id | GSE25458
| | Sample_data_row_count | 54675
| | |
|
GSM624848 | GPL570 |
|
GPR54-suppressed Ishikawa, biological rep2
|
Ishikawa cells introduced with GPR54 siRNA
|
cell line: Ishikawa
cell type: endometrial cancer cells
sirna transfection: GPR54-specific siRNA
peptide treatment: control peptide
|
Gene expression data from Ishikawa cells that were introduced with GPR54 siRNA.
|
| Sample_geo_accession | GSM624848
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Nov 17 2010
| | Sample_last_update_date | Nov 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At ~70% confluence, cells were transfected with negative control siRNA or GPR54-specific siRNA for 48 hrs, and then treated with either 10 µM metastin-10 or control peptide for the following 24 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 media with 10% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until ~70% confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix one cycle IVT.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Data were RMA normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tsukasa,,Baba
| | Sample_contact_email | babatsu@kuhp.kyoto-u.ac.jp
| | Sample_contact_department | Department of Gynecology and Obstetrics
| | Sample_contact_institute | Kyoto University Graduate School of Medicine
| | Sample_contact_address | 54 Shogoin Kawahara-cho, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624848/suppl/GSM624848.CEL.gz
| | Sample_series_id | GSE25458
| | Sample_data_row_count | 54675
| | |
|
GSM624849 | GPL570 |
|
GPR54-suppressed Ishikawa, biological rep3
|
Ishikawa cells introduced with GPR54 siRNA
|
cell line: Ishikawa
cell type: endometrial cancer cells
sirna transfection: GPR54-specific siRNA
peptide treatment: control peptide
|
Gene expression data from Ishikawa cells that were introduced with GPR54 siRNA.
|
| Sample_geo_accession | GSM624849
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Nov 17 2010
| | Sample_last_update_date | Nov 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At ~70% confluence, cells were transfected with negative control siRNA or GPR54-specific siRNA for 48 hrs, and then treated with either 10 µM metastin-10 or control peptide for the following 24 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 media with 10% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until ~70% confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix one cycle IVT.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Data were RMA normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tsukasa,,Baba
| | Sample_contact_email | babatsu@kuhp.kyoto-u.ac.jp
| | Sample_contact_department | Department of Gynecology and Obstetrics
| | Sample_contact_institute | Kyoto University Graduate School of Medicine
| | Sample_contact_address | 54 Shogoin Kawahara-cho, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624849/suppl/GSM624849.CEL.gz
| | Sample_series_id | GSE25458
| | Sample_data_row_count | 54675
| | |
|
GSM624850 | GPL570 |
|
GPR54-suppressed Ishikawa treated with metastin-10, biological rep1
|
Ishikawa cells introduced with GPR54 siRNA and treated with metastin-10
|
cell line: Ishikawa
cell type: endometrial cancer cells
sirna transfection: GPR54-specific siRNA
peptide treatment: metastin-10
|
Gene expression data from Ishikawa cells that were introduced with GPR54 siRNA and treated with metastin-10.
|
| Sample_geo_accession | GSM624850
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Nov 17 2010
| | Sample_last_update_date | Nov 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At ~70% confluence, cells were transfected with negative control siRNA or GPR54-specific siRNA for 48 hrs, and then treated with either 10 µM metastin-10 or control peptide for the following 24 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 media with 10% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until ~70% confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix one cycle IVT.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Data were RMA normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tsukasa,,Baba
| | Sample_contact_email | babatsu@kuhp.kyoto-u.ac.jp
| | Sample_contact_department | Department of Gynecology and Obstetrics
| | Sample_contact_institute | Kyoto University Graduate School of Medicine
| | Sample_contact_address | 54 Shogoin Kawahara-cho, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624850/suppl/GSM624850.CEL.gz
| | Sample_series_id | GSE25458
| | Sample_data_row_count | 54675
| | |
|
GSM624851 | GPL570 |
|
GPR54-suppressed Ishikawa treated with metastin-10, biological rep2
|
Ishikawa cells introduced with GPR54 siRNA and treated with metastin-10
|
cell line: Ishikawa
cell type: endometrial cancer cells
sirna transfection: GPR54-specific siRNA
peptide treatment: metastin-10
|
Gene expression data from Ishikawa cells that were introduced with GPR54 siRNA and treated with metastin-10.
|
| Sample_geo_accession | GSM624851
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Nov 17 2010
| | Sample_last_update_date | Nov 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At ~70% confluence, cells were transfected with negative control siRNA or GPR54-specific siRNA for 48 hrs, and then treated with either 10 µM metastin-10 or control peptide for the following 24 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 media with 10% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until ~70% confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix one cycle IVT.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Data were RMA normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tsukasa,,Baba
| | Sample_contact_email | babatsu@kuhp.kyoto-u.ac.jp
| | Sample_contact_department | Department of Gynecology and Obstetrics
| | Sample_contact_institute | Kyoto University Graduate School of Medicine
| | Sample_contact_address | 54 Shogoin Kawahara-cho, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624851/suppl/GSM624851.CEL.gz
| | Sample_series_id | GSE25458
| | Sample_data_row_count | 54675
| | |
|
GSM624852 | GPL570 |
|
GPR54-suppressed Ishikawa treated with metastin-10, biological rep3
|
Ishikawa cells introduced with GPR54 siRNA and treated with metastin-10
|
cell line: Ishikawa
cell type: endometrial cancer cells
sirna transfection: GPR54-specific siRNA
peptide treatment: metastin-10
|
Gene expression data from Ishikawa cells that were introduced with GPR54 siRNA and treated with metastin-10.
|
| Sample_geo_accession | GSM624852
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Nov 17 2010
| | Sample_last_update_date | Nov 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | At ~70% confluence, cells were transfected with negative control siRNA or GPR54-specific siRNA for 48 hrs, and then treated with either 10 µM metastin-10 or control peptide for the following 24 hrs.
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI 1640 media with 10% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until ~70% confluency.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix one cycle IVT.
| | Sample_hyb_protocol | Standard Affymetrix protocol.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Data were RMA normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tsukasa,,Baba
| | Sample_contact_email | babatsu@kuhp.kyoto-u.ac.jp
| | Sample_contact_department | Department of Gynecology and Obstetrics
| | Sample_contact_institute | Kyoto University Graduate School of Medicine
| | Sample_contact_address | 54 Shogoin Kawahara-cho, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8507
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM624nnn/GSM624852/suppl/GSM624852.CEL.gz
| | Sample_series_id | GSE25458
| | Sample_data_row_count | 54675
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Make groups for comparisons |
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| Select GSMs and click on "Add groups" |
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