Search results for the GEO ID: GSE25636  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM629877 | GPL1261 |
|
WT_Sca1+Lin-_1
|
sorting purified, Sca1+Lin-, WT
|
tissue: bone marrow
cell type: Sca1+Lin-
genotype/variation: wild type
|
|
| Sample_geo_accession | GSM629877
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Nov 28 2010
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA lineal amplification using Megascript T7 in vitro transcription kit.
| | Sample_growth_protocol_ch1 | Sorting purified Sca1+Lin- from bone marrow.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133_Plus2 chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| | Sample_data_processing | RMA (R/Bioconductor processing)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Victor,,Segura
| | Sample_contact_email | vsegura@unav.es
| | Sample_contact_department | Unit of Proteomics, Genomics and Bioinformatics
| | Sample_contact_institute | FIMA
| | Sample_contact_address | Avda. Pío XII, 55
| | Sample_contact_city | Pamplona
| | Sample_contact_state | Navarra
| | Sample_contact_zip/postal_code | 31008
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629877/suppl/GSM629877.CEL.gz
| | Sample_series_id | GSE25636
| | Sample_series_id | GSE25639
| | Sample_data_row_count | 45101
| | |
|
GSM629878 | GPL1261 |
|
WT_Sca1+Lin-_2
|
sorting purified, Sca1+Lin-, WT
|
tissue: bone marrow
cell type: Sca1+Lin-
genotype/variation: wild type
|
|
| Sample_geo_accession | GSM629878
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Nov 28 2010
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA lineal amplification using Megascript T7 in vitro transcription kit.
| | Sample_growth_protocol_ch1 | Sorting purified Sca1+Lin- from bone marrow.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133_Plus2 chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| | Sample_data_processing | RMA (R/Bioconductor processing)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Victor,,Segura
| | Sample_contact_email | vsegura@unav.es
| | Sample_contact_department | Unit of Proteomics, Genomics and Bioinformatics
| | Sample_contact_institute | FIMA
| | Sample_contact_address | Avda. Pío XII, 55
| | Sample_contact_city | Pamplona
| | Sample_contact_state | Navarra
| | Sample_contact_zip/postal_code | 31008
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629878/suppl/GSM629878.CEL.gz
| | Sample_series_id | GSE25636
| | Sample_series_id | GSE25639
| | Sample_data_row_count | 45101
| | |
|
GSM629879 | GPL1261 |
|
WT_Sca1+Lin-_3
|
sorting purified, Sca1+Lin-, WT
|
tissue: bone marrow
cell type: Sca1+Lin-
genotype/variation: wild type
|
|
| Sample_geo_accession | GSM629879
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Nov 28 2010
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA lineal amplification using Megascript T7 in vitro transcription kit.
| | Sample_growth_protocol_ch1 | Sorting purified Sca1+Lin- from bone marrow.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133_Plus2 chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| | Sample_data_processing | RMA (R/Bioconductor processing)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Victor,,Segura
| | Sample_contact_email | vsegura@unav.es
| | Sample_contact_department | Unit of Proteomics, Genomics and Bioinformatics
| | Sample_contact_institute | FIMA
| | Sample_contact_address | Avda. Pío XII, 55
| | Sample_contact_city | Pamplona
| | Sample_contact_state | Navarra
| | Sample_contact_zip/postal_code | 31008
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629879/suppl/GSM629879.CEL.gz
| | Sample_series_id | GSE25636
| | Sample_series_id | GSE25639
| | Sample_data_row_count | 45101
| | |
|
GSM629880 | GPL1261 |
|
Sca1-MALT1_Sca1+Lin-_1
|
sorting purified, Sca1+Lin-, Sca1-MALT1
|
tissue: bone marrow
cell type: Sca1+Lin-
genotype/variation: Sca1-MALT1
|
|
| Sample_geo_accession | GSM629880
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Nov 28 2010
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA lineal amplification using Megascript T7 in vitro transcription kit.
| | Sample_growth_protocol_ch1 | Sorting purified Sca1+Lin- from bone marrow.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133_Plus2 chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| | Sample_data_processing | RMA (R/Bioconductor processing)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Victor,,Segura
| | Sample_contact_email | vsegura@unav.es
| | Sample_contact_department | Unit of Proteomics, Genomics and Bioinformatics
| | Sample_contact_institute | FIMA
| | Sample_contact_address | Avda. Pío XII, 55
| | Sample_contact_city | Pamplona
| | Sample_contact_state | Navarra
| | Sample_contact_zip/postal_code | 31008
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629880/suppl/GSM629880.CEL.gz
| | Sample_series_id | GSE25636
| | Sample_series_id | GSE25639
| | Sample_data_row_count | 45101
| | |
|
GSM629881 | GPL1261 |
|
Sca1-MALT1_Sca1+Lin-_2
|
sorting purified, Sca1+Lin-, Sca1-MALT1
|
tissue: bone marrow
cell type: Sca1+Lin-
genotype/variation: Sca1-MALT1
|
|
| Sample_geo_accession | GSM629881
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Nov 28 2010
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA lineal amplification using Megascript T7 in vitro transcription kit.
| | Sample_growth_protocol_ch1 | Sorting purified Sca1+Lin- from bone marrow.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133_Plus2 chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| | Sample_data_processing | RMA (R/Bioconductor processing)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Victor,,Segura
| | Sample_contact_email | vsegura@unav.es
| | Sample_contact_department | Unit of Proteomics, Genomics and Bioinformatics
| | Sample_contact_institute | FIMA
| | Sample_contact_address | Avda. Pío XII, 55
| | Sample_contact_city | Pamplona
| | Sample_contact_state | Navarra
| | Sample_contact_zip/postal_code | 31008
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629881/suppl/GSM629881.CEL.gz
| | Sample_series_id | GSE25636
| | Sample_series_id | GSE25639
| | Sample_data_row_count | 45101
| | |
|
GSM629882 | GPL1261 |
|
Sca1-MALT1_Sca1+Lin-_3
|
sorting purified, Sca1+Lin-, Sca1-MALT1
|
tissue: bone marrow
cell type: Sca1+Lin-
genotype/variation: Sca1-MALT1
|
|
| Sample_geo_accession | GSM629882
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Nov 28 2010
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA lineal amplification using Megascript T7 in vitro transcription kit.
| | Sample_growth_protocol_ch1 | Sorting purified Sca1+Lin- from bone marrow.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133_Plus2 chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| | Sample_data_processing | RMA (R/Bioconductor processing)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Victor,,Segura
| | Sample_contact_email | vsegura@unav.es
| | Sample_contact_department | Unit of Proteomics, Genomics and Bioinformatics
| | Sample_contact_institute | FIMA
| | Sample_contact_address | Avda. Pío XII, 55
| | Sample_contact_city | Pamplona
| | Sample_contact_state | Navarra
| | Sample_contact_zip/postal_code | 31008
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629882/suppl/GSM629882.CEL.gz
| | Sample_series_id | GSE25636
| | Sample_series_id | GSE25639
| | Sample_data_row_count | 45101
| | |
|
GSM629883 | GPL1261 |
|
Sca1-MALT1_Sca1+Lin-_4
|
sorting purified, Sca1+Lin-, Sca1-MALT1
|
tissue: bone marrow
cell type: Sca1+Lin-
genotype/variation: Sca1-MALT1
|
|
| Sample_geo_accession | GSM629883
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Nov 28 2010
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA lineal amplification using Megascript T7 in vitro transcription kit.
| | Sample_growth_protocol_ch1 | Sorting purified Sca1+Lin- from bone marrow.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133_Plus2 chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| | Sample_data_processing | RMA (R/Bioconductor processing)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Victor,,Segura
| | Sample_contact_email | vsegura@unav.es
| | Sample_contact_department | Unit of Proteomics, Genomics and Bioinformatics
| | Sample_contact_institute | FIMA
| | Sample_contact_address | Avda. Pío XII, 55
| | Sample_contact_city | Pamplona
| | Sample_contact_state | Navarra
| | Sample_contact_zip/postal_code | 31008
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629883/suppl/GSM629883.CEL.gz
| | Sample_series_id | GSE25636
| | Sample_series_id | GSE25639
| | Sample_data_row_count | 45101
| | |
|
GSM629884 | GPL1261 |
|
Sca1-MALT1_Sca1+Lin-_5
|
sorting purified, Sca1+Lin-, Sca1-MALT1
|
tissue: bone marrow
cell type: Sca1+Lin-
genotype/variation: Sca1-MALT1
|
|
| Sample_geo_accession | GSM629884
| | Sample_status | Public on Jul 10 2012
| | Sample_submission_date | Nov 28 2010
| | Sample_last_update_date | Jul 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA lineal amplification using Megascript T7 in vitro transcription kit.
| | Sample_growth_protocol_ch1 | Sorting purified Sca1+Lin- from bone marrow.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133_Plus2 chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| | Sample_data_processing | RMA (R/Bioconductor processing)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Victor,,Segura
| | Sample_contact_email | vsegura@unav.es
| | Sample_contact_department | Unit of Proteomics, Genomics and Bioinformatics
| | Sample_contact_institute | FIMA
| | Sample_contact_address | Avda. Pío XII, 55
| | Sample_contact_city | Pamplona
| | Sample_contact_state | Navarra
| | Sample_contact_zip/postal_code | 31008
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629884/suppl/GSM629884.CEL.gz
| | Sample_series_id | GSE25636
| | Sample_series_id | GSE25639
| | Sample_data_row_count | 45101
| | |
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