Search results for the GEO ID: GSE25640 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM629991 | GPL1261 |
|
Phan_001_WT.saline rep1
|
saline injected mouse lung
|
strain: C57BL/6
tissue: Lung
agent: saline
genotype/variation: wild type
|
gene expression profile at day 21 after saline endotracheal injection
|
Sample_geo_accession | GSM629991
| Sample_status | Public on Nov 29 2010
| Sample_submission_date | Nov 28 2010
| Sample_last_update_date | Nov 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Bleomycin or saline was endotracheally injected into wild type and FIZZ2 knockout mice, then the lungs were purfused with PBS and harvested for total RNA isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Tianju,,Liu
| Sample_contact_email | tianliu@umich.edu
| Sample_contact_phone | (734)763-5731
| Sample_contact_fax | (734)615-2331
| Sample_contact_department | Pathology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 109 Zina pitcher pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | Michigan
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629991/suppl/GSM629991.CEL.gz
| Sample_series_id | GSE25640
| Sample_data_row_count | 45101
| |
|
GSM629992 | GPL1261 |
|
Phan_002_WT.saline rep2
|
saline injected mouse lung
|
strain: C57BL/6
tissue: Lung
agent: saline
genotype/variation: wild type
|
gene expression profile at day 21 after saline endotracheal injection
|
Sample_geo_accession | GSM629992
| Sample_status | Public on Nov 29 2010
| Sample_submission_date | Nov 28 2010
| Sample_last_update_date | Nov 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Bleomycin or saline was endotracheally injected into wild type and FIZZ2 knockout mice, then the lungs were purfused with PBS and harvested for total RNA isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Tianju,,Liu
| Sample_contact_email | tianliu@umich.edu
| Sample_contact_phone | (734)763-5731
| Sample_contact_fax | (734)615-2331
| Sample_contact_department | Pathology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 109 Zina pitcher pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | Michigan
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629992/suppl/GSM629992.CEL.gz
| Sample_series_id | GSE25640
| Sample_data_row_count | 45101
| |
|
GSM629993 | GPL1261 |
|
Phan_003_WT.saline rep3
|
saline injected mouse lung
|
strain: C57BL/6
tissue: Lung
agent: saline
genotype/variation: wild type
|
gene expression profile at day 21 after saline endotracheal injection
|
Sample_geo_accession | GSM629993
| Sample_status | Public on Nov 29 2010
| Sample_submission_date | Nov 28 2010
| Sample_last_update_date | Nov 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Bleomycin or saline was endotracheally injected into wild type and FIZZ2 knockout mice, then the lungs were purfused with PBS and harvested for total RNA isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Tianju,,Liu
| Sample_contact_email | tianliu@umich.edu
| Sample_contact_phone | (734)763-5731
| Sample_contact_fax | (734)615-2331
| Sample_contact_department | Pathology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 109 Zina pitcher pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | Michigan
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629993/suppl/GSM629993.CEL.gz
| Sample_series_id | GSE25640
| Sample_data_row_count | 45101
| |
|
GSM629994 | GPL1261 |
|
Phan_004_WT.bleomycin rep1
|
bleomycin injected mouse lung
|
strain: C57BL/6
tissue: Lung
agent: bleomycin
genotype/variation: wild type
|
gene expression profile at day 21 after bleomycin endotracheal injection
|
Sample_geo_accession | GSM629994
| Sample_status | Public on Nov 29 2010
| Sample_submission_date | Nov 28 2010
| Sample_last_update_date | Nov 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Bleomycin or saline was endotracheally injected into wild type and FIZZ2 knockout mice, then the lungs were purfused with PBS and harvested for total RNA isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Tianju,,Liu
| Sample_contact_email | tianliu@umich.edu
| Sample_contact_phone | (734)763-5731
| Sample_contact_fax | (734)615-2331
| Sample_contact_department | Pathology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 109 Zina pitcher pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | Michigan
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629994/suppl/GSM629994.CEL.gz
| Sample_series_id | GSE25640
| Sample_data_row_count | 45101
| |
|
GSM629995 | GPL1261 |
|
Phan_005_WT.bleomycin rep2
|
bleomycin injected mouse lung
|
strain: C57BL/6
tissue: Lung
agent: bleomycin
genotype/variation: wild type
|
gene expression profile at day 21 after bleomycin endotracheal injection
|
Sample_geo_accession | GSM629995
| Sample_status | Public on Nov 29 2010
| Sample_submission_date | Nov 28 2010
| Sample_last_update_date | Nov 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Bleomycin or saline was endotracheally injected into wild type and FIZZ2 knockout mice, then the lungs were purfused with PBS and harvested for total RNA isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Tianju,,Liu
| Sample_contact_email | tianliu@umich.edu
| Sample_contact_phone | (734)763-5731
| Sample_contact_fax | (734)615-2331
| Sample_contact_department | Pathology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 109 Zina pitcher pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | Michigan
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629995/suppl/GSM629995.CEL.gz
| Sample_series_id | GSE25640
| Sample_data_row_count | 45101
| |
|
GSM629996 | GPL1261 |
|
Phan_006_WT.bleomycin rep3
|
bleomycin injected mouse lung
|
strain: C57BL/6
tissue: Lung
agent: bleomycin
genotype/variation: wild type
|
gene expression profile at day 21 after bleomycin endotracheal injection
|
Sample_geo_accession | GSM629996
| Sample_status | Public on Nov 29 2010
| Sample_submission_date | Nov 28 2010
| Sample_last_update_date | Nov 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Bleomycin or saline was endotracheally injected into wild type and FIZZ2 knockout mice, then the lungs were purfused with PBS and harvested for total RNA isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Tianju,,Liu
| Sample_contact_email | tianliu@umich.edu
| Sample_contact_phone | (734)763-5731
| Sample_contact_fax | (734)615-2331
| Sample_contact_department | Pathology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 109 Zina pitcher pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | Michigan
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629996/suppl/GSM629996.CEL.gz
| Sample_series_id | GSE25640
| Sample_data_row_count | 45101
| |
|
GSM629997 | GPL1261 |
|
Phan_007_KO.saline rep1
|
saline injected mouse lung
|
strain: C57BL/6
tissue: Lung
agent: saline
genotype/variation: FIZZ2 knockout
|
gene expression profile at day 21 after saline endotracheal injection
|
Sample_geo_accession | GSM629997
| Sample_status | Public on Nov 29 2010
| Sample_submission_date | Nov 28 2010
| Sample_last_update_date | Nov 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Bleomycin or saline was endotracheally injected into wild type and FIZZ2 knockout mice, then the lungs were purfused with PBS and harvested for total RNA isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Tianju,,Liu
| Sample_contact_email | tianliu@umich.edu
| Sample_contact_phone | (734)763-5731
| Sample_contact_fax | (734)615-2331
| Sample_contact_department | Pathology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 109 Zina pitcher pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | Michigan
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629997/suppl/GSM629997.CEL.gz
| Sample_series_id | GSE25640
| Sample_data_row_count | 45101
| |
|
GSM629998 | GPL1261 |
|
Phan_008_KO.saline rep2
|
saline injected mouse lung
|
strain: C57BL/6
tissue: Lung
agent: saline
genotype/variation: FIZZ2 knockout
|
gene expression profile at day 21 after saline endotracheal injection
|
Sample_geo_accession | GSM629998
| Sample_status | Public on Nov 29 2010
| Sample_submission_date | Nov 28 2010
| Sample_last_update_date | Nov 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Bleomycin or saline was endotracheally injected into wild type and FIZZ2 knockout mice, then the lungs were purfused with PBS and harvested for total RNA isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Tianju,,Liu
| Sample_contact_email | tianliu@umich.edu
| Sample_contact_phone | (734)763-5731
| Sample_contact_fax | (734)615-2331
| Sample_contact_department | Pathology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 109 Zina pitcher pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | Michigan
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629998/suppl/GSM629998.CEL.gz
| Sample_series_id | GSE25640
| Sample_data_row_count | 45101
| |
|
GSM629999 | GPL1261 |
|
Phan_009_KO.saline rep3
|
saline injected mouse lung
|
strain: C57BL/6
tissue: Lung
agent: saline
genotype/variation: FIZZ2 knockout
|
gene expression profile at day 21 after saline endotracheal injection
|
Sample_geo_accession | GSM629999
| Sample_status | Public on Nov 29 2010
| Sample_submission_date | Nov 28 2010
| Sample_last_update_date | Nov 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Bleomycin or saline was endotracheally injected into wild type and FIZZ2 knockout mice, then the lungs were purfused with PBS and harvested for total RNA isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Tianju,,Liu
| Sample_contact_email | tianliu@umich.edu
| Sample_contact_phone | (734)763-5731
| Sample_contact_fax | (734)615-2331
| Sample_contact_department | Pathology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 109 Zina pitcher pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | Michigan
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM629nnn/GSM629999/suppl/GSM629999.CEL.gz
| Sample_series_id | GSE25640
| Sample_data_row_count | 45101
| |
|
GSM630000 | GPL1261 |
|
Phan_010_KO.bleomycin rep1
|
bleomycin injected mouse lung
|
strain: C57BL/6
tissue: Lung
agent: bleomycin
genotype/variation: FIZZ2 knockout
|
gene expression profile at day 21 after bleomycin endotracheal injection
|
Sample_geo_accession | GSM630000
| Sample_status | Public on Nov 29 2010
| Sample_submission_date | Nov 28 2010
| Sample_last_update_date | Nov 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Bleomycin or saline was endotracheally injected into wild type and FIZZ2 knockout mice, then the lungs were purfused with PBS and harvested for total RNA isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Tianju,,Liu
| Sample_contact_email | tianliu@umich.edu
| Sample_contact_phone | (734)763-5731
| Sample_contact_fax | (734)615-2331
| Sample_contact_department | Pathology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 109 Zina pitcher pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | Michigan
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM630nnn/GSM630000/suppl/GSM630000.CEL.gz
| Sample_series_id | GSE25640
| Sample_data_row_count | 45101
| |
|
GSM630001 | GPL1261 |
|
Phan_011_KO.bleomycin rep2
|
bleomycin injected mouse lung
|
strain: C57BL/6
tissue: Lung
agent: bleomycin
genotype/variation: FIZZ2 knockout
|
gene expression profile at day 21 after bleomycin endotracheal injection
|
Sample_geo_accession | GSM630001
| Sample_status | Public on Nov 29 2010
| Sample_submission_date | Nov 28 2010
| Sample_last_update_date | Nov 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Bleomycin or saline was endotracheally injected into wild type and FIZZ2 knockout mice, then the lungs were purfused with PBS and harvested for total RNA isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Tianju,,Liu
| Sample_contact_email | tianliu@umich.edu
| Sample_contact_phone | (734)763-5731
| Sample_contact_fax | (734)615-2331
| Sample_contact_department | Pathology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 109 Zina pitcher pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | Michigan
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM630nnn/GSM630001/suppl/GSM630001.CEL.gz
| Sample_series_id | GSE25640
| Sample_data_row_count | 45101
| |
|
GSM630002 | GPL1261 |
|
Phan_012_KO.bleomycin rep3
|
bleomycin injected mouse lung
|
strain: C57BL/6
tissue: Lung
agent: bleomycin
genotype/variation: FIZZ2 knockout
|
gene expression profile at day 21 after bleomycin endotracheal injection
|
Sample_geo_accession | GSM630002
| Sample_status | Public on Nov 29 2010
| Sample_submission_date | Nov 28 2010
| Sample_last_update_date | Nov 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Bleomycin or saline was endotracheally injected into wild type and FIZZ2 knockout mice, then the lungs were purfused with PBS and harvested for total RNA isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| Sample_data_processing | Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical language. RMA was used to normalize the data and fit log2 transformed expression values.
| Sample_platform_id | GPL1261
| Sample_contact_name | Tianju,,Liu
| Sample_contact_email | tianliu@umich.edu
| Sample_contact_phone | (734)763-5731
| Sample_contact_fax | (734)615-2331
| Sample_contact_department | Pathology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 109 Zina pitcher pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | Michigan
| Sample_contact_zip/postal_code | 48109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM630nnn/GSM630002/suppl/GSM630002.CEL.gz
| Sample_series_id | GSE25640
| Sample_data_row_count | 45101
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