Search results for the GEO ID: GSE25724  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM631755 | GPL96 |
|
Non-diabetic islets, rep1
|
human islets, non-diabetic
|
tissue: pancreatic islets
disease state: non-diabetic
age: 47 yrs
gender: male
bmi (kg/m2): 27.7
|
ND1
Gene expression data from non-diabetic human islets.
|
| Sample_geo_accession | GSM631755
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Nov 30 2010
| | Sample_last_update_date | Nov 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Once isolated, human islets were cultured in M199 culture medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA after two-cycle target labeling (GeneChip Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HG U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with R/Bioconductor using affy, affyPLM for quality control, rma for normalization and limma for differential expression analysis.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Marco,,Bugliani
| | Sample_contact_department | Dept. Endocrinology and Metabolism
| | Sample_contact_institute | University of Pisa
| | Sample_contact_address | via Paradisa, 2
| | Sample_contact_city | Pisa
| | Sample_contact_zip/postal_code | 56124
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631755/suppl/GSM631755.CEL.gz
| | Sample_series_id | GSE25724
| | Sample_data_row_count | 22283
| | |
|
GSM631756 | GPL96 |
|
Non-diabetic islets, rep2
|
human islets, non-diabetic
|
tissue: pancreatic islets
disease state: non-diabetic
age: 33 yrs
gender: male
bmi (kg/m2): 22.9
|
ND2
Gene expression data from non-diabetic human islets.
|
| Sample_geo_accession | GSM631756
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Nov 30 2010
| | Sample_last_update_date | Nov 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Once isolated, human islets were cultured in M199 culture medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA after two-cycle target labeling (GeneChip Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HG U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with R/Bioconductor using affy, affyPLM for quality control, rma for normalization and limma for differential expression analysis.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Marco,,Bugliani
| | Sample_contact_department | Dept. Endocrinology and Metabolism
| | Sample_contact_institute | University of Pisa
| | Sample_contact_address | via Paradisa, 2
| | Sample_contact_city | Pisa
| | Sample_contact_zip/postal_code | 56124
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631756/suppl/GSM631756.CEL.gz
| | Sample_series_id | GSE25724
| | Sample_data_row_count | 22283
| | |
|
GSM631757 | GPL96 |
|
Non-diabetic islets, rep3
|
human islets, non-diabetic
|
tissue: pancreatic islets
disease state: non-diabetic
age: 47 yrs
gender: male
bmi (kg/m2): 28.4
|
ND3
Gene expression data from non-diabetic human islets.
|
| Sample_geo_accession | GSM631757
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Nov 30 2010
| | Sample_last_update_date | Nov 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Once isolated, human islets were cultured in M199 culture medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA after two-cycle target labeling (GeneChip Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HG U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with R/Bioconductor using affy, affyPLM for quality control, rma for normalization and limma for differential expression analysis.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Marco,,Bugliani
| | Sample_contact_department | Dept. Endocrinology and Metabolism
| | Sample_contact_institute | University of Pisa
| | Sample_contact_address | via Paradisa, 2
| | Sample_contact_city | Pisa
| | Sample_contact_zip/postal_code | 56124
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631757/suppl/GSM631757.CEL.gz
| | Sample_series_id | GSE25724
| | Sample_data_row_count | 22283
| | |
|
GSM631758 | GPL96 |
|
Non-diabetic islets, rep4
|
human islets, non-diabetic
|
tissue: pancreatic islets
disease state: non-diabetic
age: 54 yrs
gender: male
bmi (kg/m2): 23.1
|
ND4
Gene expression data from non-diabetic human islets.
|
| Sample_geo_accession | GSM631758
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Nov 30 2010
| | Sample_last_update_date | Nov 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Once isolated, human islets were cultured in M199 culture medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA after two-cycle target labeling (GeneChip Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HG U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with R/Bioconductor using affy, affyPLM for quality control, rma for normalization and limma for differential expression analysis.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Marco,,Bugliani
| | Sample_contact_department | Dept. Endocrinology and Metabolism
| | Sample_contact_institute | University of Pisa
| | Sample_contact_address | via Paradisa, 2
| | Sample_contact_city | Pisa
| | Sample_contact_zip/postal_code | 56124
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631758/suppl/GSM631758.CEL.gz
| | Sample_series_id | GSE25724
| | Sample_data_row_count | 22283
| | |
|
GSM631759 | GPL96 |
|
Non-diabetic islets, rep5
|
human islets, non-diabetic
|
tissue: pancreatic islets
disease state: non-diabetic
age: 76 yrs
gender: female
bmi (kg/m2): 25.9
|
ND5
Gene expression data from non-diabetic human islets.
|
| Sample_geo_accession | GSM631759
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Nov 30 2010
| | Sample_last_update_date | Nov 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Once isolated, human islets were cultured in M199 culture medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA after two-cycle target labeling (GeneChip Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HG U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with R/Bioconductor using affy, affyPLM for quality control, rma for normalization and limma for differential expression analysis.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Marco,,Bugliani
| | Sample_contact_department | Dept. Endocrinology and Metabolism
| | Sample_contact_institute | University of Pisa
| | Sample_contact_address | via Paradisa, 2
| | Sample_contact_city | Pisa
| | Sample_contact_zip/postal_code | 56124
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631759/suppl/GSM631759.CEL.gz
| | Sample_series_id | GSE25724
| | Sample_data_row_count | 22283
| | |
|
GSM631760 | GPL96 |
|
Non-diabetic islets, rep6
|
human islets, non-diabetic
|
tissue: pancreatic islets
disease state: non-diabetic
age: 77 yrs
gender: female
bmi (kg/m2): 23.8
|
ND6
Gene expression data from non-diabetic human islets.
|
| Sample_geo_accession | GSM631760
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Nov 30 2010
| | Sample_last_update_date | Nov 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Once isolated, human islets were cultured in M199 culture medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA after two-cycle target labeling (GeneChip Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HG U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with R/Bioconductor using affy, affyPLM for quality control, rma for normalization and limma for differential expression analysis.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Marco,,Bugliani
| | Sample_contact_department | Dept. Endocrinology and Metabolism
| | Sample_contact_institute | University of Pisa
| | Sample_contact_address | via Paradisa, 2
| | Sample_contact_city | Pisa
| | Sample_contact_zip/postal_code | 56124
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631760/suppl/GSM631760.CEL.gz
| | Sample_series_id | GSE25724
| | Sample_data_row_count | 22283
| | |
|
GSM631761 | GPL96 |
|
Non-diabetic islets, rep7
|
human islets, non-diabetic
|
tissue: pancreatic islets
disease state: non-diabetic
age: 73 yrs
gender: female
bmi (kg/m2): 22
|
ND8
Gene expression data from non-diabetic human islets.
|
| Sample_geo_accession | GSM631761
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Nov 30 2010
| | Sample_last_update_date | Nov 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Once isolated, human islets were cultured in M199 culture medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA after two-cycle target labeling (GeneChip Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HG U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with R/Bioconductor using affy, affyPLM for quality control, rma for normalization and limma for differential expression analysis.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Marco,,Bugliani
| | Sample_contact_department | Dept. Endocrinology and Metabolism
| | Sample_contact_institute | University of Pisa
| | Sample_contact_address | via Paradisa, 2
| | Sample_contact_city | Pisa
| | Sample_contact_zip/postal_code | 56124
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631761/suppl/GSM631761.CEL.gz
| | Sample_series_id | GSE25724
| | Sample_data_row_count | 22283
| | |
|
GSM631762 | GPL96 |
|
Type 2 diabetic islets, rep1
|
human islets, diabetic
|
tissue: pancreatic islets
disease state: type 2 diabetes
age: 79 yrs
gender: male
bmi (kg/m2): 27.5
|
T2D (B)
Gene expression data from type 2 diabetic human islets.
|
| Sample_geo_accession | GSM631762
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Nov 30 2010
| | Sample_last_update_date | Nov 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Once isolated, human islets were cultured in M199 culture medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA after two-cycle target labeling (GeneChip Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HG U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with R/Bioconductor using affy, affyPLM for quality control, rma for normalization and limma for differential expression analysis.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Marco,,Bugliani
| | Sample_contact_department | Dept. Endocrinology and Metabolism
| | Sample_contact_institute | University of Pisa
| | Sample_contact_address | via Paradisa, 2
| | Sample_contact_city | Pisa
| | Sample_contact_zip/postal_code | 56124
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631762/suppl/GSM631762.CEL.gz
| | Sample_series_id | GSE25724
| | Sample_data_row_count | 22283
| | |
|
GSM631763 | GPL96 |
|
Type 2 diabetic islets, rep2
|
human islets, diabetic
|
tissue: pancreatic islets
disease state: type 2 diabetes
age: 76 yrs
gender: male
bmi (kg/m2): 26
|
T2D (C)
Gene expression data from type 2 diabetic human islets.
|
| Sample_geo_accession | GSM631763
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Nov 30 2010
| | Sample_last_update_date | Nov 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Once isolated, human islets were cultured in M199 culture medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA after two-cycle target labeling (GeneChip Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HG U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with R/Bioconductor using affy, affyPLM for quality control, rma for normalization and limma for differential expression analysis.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Marco,,Bugliani
| | Sample_contact_department | Dept. Endocrinology and Metabolism
| | Sample_contact_institute | University of Pisa
| | Sample_contact_address | via Paradisa, 2
| | Sample_contact_city | Pisa
| | Sample_contact_zip/postal_code | 56124
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631763/suppl/GSM631763.CEL.gz
| | Sample_series_id | GSE25724
| | Sample_data_row_count | 22283
| | |
|
GSM631764 | GPL96 |
|
Type 2 diabetic islets, rep3
|
human islets, diabetic
|
tissue: pancreatic islets
disease state: type 2 diabetes
age: 73 yrs
gender: female
bmi (kg/m2): 29
|
T2D (D)
Gene expression data from type 2 diabetic human islets.
|
| Sample_geo_accession | GSM631764
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Nov 30 2010
| | Sample_last_update_date | Nov 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Once isolated, human islets were cultured in M199 culture medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA after two-cycle target labeling (GeneChip Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HG U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with R/Bioconductor using affy, affyPLM for quality control, rma for normalization and limma for differential expression analysis.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Marco,,Bugliani
| | Sample_contact_department | Dept. Endocrinology and Metabolism
| | Sample_contact_institute | University of Pisa
| | Sample_contact_address | via Paradisa, 2
| | Sample_contact_city | Pisa
| | Sample_contact_zip/postal_code | 56124
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631764/suppl/GSM631764.CEL.gz
| | Sample_series_id | GSE25724
| | Sample_data_row_count | 22283
| | |
|
GSM631765 | GPL96 |
|
Type 2 diabetic islets, rep4
|
human islets, diabetic
|
tissue: pancreatic islets
disease state: type 2 diabetes
age: 75 yrs
gender: female
bmi (kg/m2): 26.5
|
T2D (E)
Gene expression data from type 2 diabetic human islets.
|
| Sample_geo_accession | GSM631765
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Nov 30 2010
| | Sample_last_update_date | Nov 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Once isolated, human islets were cultured in M199 culture medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA after two-cycle target labeling (GeneChip Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HG U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with R/Bioconductor using affy, affyPLM for quality control, rma for normalization and limma for differential expression analysis.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Marco,,Bugliani
| | Sample_contact_department | Dept. Endocrinology and Metabolism
| | Sample_contact_institute | University of Pisa
| | Sample_contact_address | via Paradisa, 2
| | Sample_contact_city | Pisa
| | Sample_contact_zip/postal_code | 56124
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631765/suppl/GSM631765.CEL.gz
| | Sample_series_id | GSE25724
| | Sample_data_row_count | 22283
| | |
|
GSM631766 | GPL96 |
|
Type 2 diabetic islets, rep5
|
human islets, diabetic
|
tissue: pancreatic islets
disease state: type 2 diabetes
age: 54 yrs
gender: female
bmi (kg/m2): 23.9
|
T2D (F)
Gene expression data from type 2 diabetic human islets.
|
| Sample_geo_accession | GSM631766
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Nov 30 2010
| | Sample_last_update_date | Nov 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Once isolated, human islets were cultured in M199 culture medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA after two-cycle target labeling (GeneChip Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HG U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with R/Bioconductor using affy, affyPLM for quality control, rma for normalization and limma for differential expression analysis.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Marco,,Bugliani
| | Sample_contact_department | Dept. Endocrinology and Metabolism
| | Sample_contact_institute | University of Pisa
| | Sample_contact_address | via Paradisa, 2
| | Sample_contact_city | Pisa
| | Sample_contact_zip/postal_code | 56124
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631766/suppl/GSM631766.CEL.gz
| | Sample_series_id | GSE25724
| | Sample_data_row_count | 22283
| | |
|
GSM631767 | GPL96 |
|
Type 2 diabetic islets, rep6
|
human islets, diabetic
|
tissue: pancreatic islets
disease state: type 2 diabetes
age: 66 yrs
gender: male
bmi (kg/m2): 23.1
|
T2D (I)
Gene expression data from type 2 diabetic human islets.
|
| Sample_geo_accession | GSM631767
| | Sample_status | Public on Dec 01 2010
| | Sample_submission_date | Nov 30 2010
| | Sample_last_update_date | Nov 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Once isolated, human islets were cultured in M199 culture medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA after two-cycle target labeling (GeneChip Expression Analysis Technical Manual, 2006, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip HG U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with R/Bioconductor using affy, affyPLM for quality control, rma for normalization and limma for differential expression analysis.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Marco,,Bugliani
| | Sample_contact_department | Dept. Endocrinology and Metabolism
| | Sample_contact_institute | University of Pisa
| | Sample_contact_address | via Paradisa, 2
| | Sample_contact_city | Pisa
| | Sample_contact_zip/postal_code | 56124
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631767/suppl/GSM631767.CEL.gz
| | Sample_series_id | GSE25724
| | Sample_data_row_count | 22283
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
