Result

Search results for the GEO ID: GSE25725

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM631750

GPL570

FP_CEP701_16hrs_1

cep701 treated HEL cells

treatment: cep701 cell line: HEL cells

Sample_geo_accession	GSM631750
Sample_status	Public on Jan 01 2011
Sample_submission_date	Nov 30 2010
Sample_last_update_date	Jan 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	0.5 micromolar of cep701 is added to the media for 16 hours. The lentivirus pLKO vector expression PRMT5 shRNA (TRCN0000107086) was used to make stable cell line knocking down PRMt5.
Sample_growth_protocol_ch1	10% fetal bovine serum with RPMI media, 37 degree 5% CO2 incubator
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction plus kit was used to isolate RNA, and cDNA was made with invitrogen superscriptase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
Sample_hyb_protocol	10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Sample_scan_protocol	Affymetrix GeneArray scanner 7G
Sample_data_processing	Feature intensity was extracted and processed using MAS5 by Affymetrix GeneChip Command Console as CEL files. CHP files are generated by Affymetrix Expression Console.
Sample_platform_id	GPL570
Sample_contact_name	Jeffrey,,Zhao
Sample_contact_email	zhaoy@mskcc.org
Sample_contact_department	Genomics Core
Sample_contact_institute	Memorial Sloan Kettering Cancer Center
Sample_contact_address	1275 York Avenue
Sample_contact_city	New York
Sample_contact_state	RRL417A
Sample_contact_zip/postal_code	10065
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631750/suppl/GSM631750.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631750/suppl/GSM631750.CHP.gz
Sample_series_id	GSE25725
Sample_data_row_count	54675

GSM631751

GPL570

FP_CEP701_16hrs_2

cep701 treated HEL cells

treatment: cep701 cell line: HEL cells

Sample_geo_accession	GSM631751
Sample_status	Public on Jan 01 2011
Sample_submission_date	Nov 30 2010
Sample_last_update_date	Jan 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	0.5 micromolar of cep701 is added to the media for 16 hours. The lentivirus pLKO vector expression PRMT5 shRNA (TRCN0000107086) was used to make stable cell line knocking down PRMt5.
Sample_growth_protocol_ch1	10% fetal bovine serum with RPMI media, 37 degree 5% CO2 incubator
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction plus kit was used to isolate RNA, and cDNA was made with invitrogen superscriptase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
Sample_hyb_protocol	10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Sample_scan_protocol	Affymetrix GeneArray scanner 7G
Sample_data_processing	Feature intensity was extracted and processed using MAS5 by Affymetrix GeneChip Command Console as CEL files. CHP files are generated by Affymetrix Expression Console.
Sample_platform_id	GPL570
Sample_contact_name	Jeffrey,,Zhao
Sample_contact_email	zhaoy@mskcc.org
Sample_contact_department	Genomics Core
Sample_contact_institute	Memorial Sloan Kettering Cancer Center
Sample_contact_address	1275 York Avenue
Sample_contact_city	New York
Sample_contact_state	RRL417A
Sample_contact_zip/postal_code	10065
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631751/suppl/GSM631751.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631751/suppl/GSM631751.CHP.gz
Sample_series_id	GSE25725
Sample_data_row_count	54675

GSM631752

GPL570

FP_DMSO_16hrs_1

DMSO treated HEL cells

treatment: DMSO cell line: HEL cells

Sample_geo_accession	GSM631752
Sample_status	Public on Jan 01 2011
Sample_submission_date	Nov 30 2010
Sample_last_update_date	Jan 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	0.5 micromolar of cep701 is added to the media for 16 hours. The lentivirus pLKO vector expression PRMT5 shRNA (TRCN0000107086) was used to make stable cell line knocking down PRMt5.
Sample_growth_protocol_ch1	10% fetal bovine serum with RPMI media, 37 degree 5% CO2 incubator
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction plus kit was used to isolate RNA, and cDNA was made with invitrogen superscriptase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
Sample_hyb_protocol	10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Sample_scan_protocol	Affymetrix GeneArray scanner 7G
Sample_data_processing	Feature intensity was extracted and processed using MAS5 by Affymetrix GeneChip Command Console as CEL files. CHP files are generated by Affymetrix Expression Console.
Sample_platform_id	GPL570
Sample_contact_name	Jeffrey,,Zhao
Sample_contact_email	zhaoy@mskcc.org
Sample_contact_department	Genomics Core
Sample_contact_institute	Memorial Sloan Kettering Cancer Center
Sample_contact_address	1275 York Avenue
Sample_contact_city	New York
Sample_contact_state	RRL417A
Sample_contact_zip/postal_code	10065
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631752/suppl/GSM631752.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631752/suppl/GSM631752.CHP.gz
Sample_series_id	GSE25725
Sample_data_row_count	54675

GSM631753

GPL570

FP_LKO_scramble_111709

HEL cells with scramble shRNA

genotype/variation: scramble shRNA cell line: HEL cells

Sample_geo_accession	GSM631753
Sample_status	Public on Jan 01 2011
Sample_submission_date	Nov 30 2010
Sample_last_update_date	Jan 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	0.5 micromolar of cep701 is added to the media for 16 hours. The lentivirus pLKO vector expression PRMT5 shRNA (TRCN0000107086) was used to make stable cell line knocking down PRMt5.
Sample_growth_protocol_ch1	10% fetal bovine serum with RPMI media, 37 degree 5% CO2 incubator
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction plus kit was used to isolate RNA, and cDNA was made with invitrogen superscriptase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
Sample_hyb_protocol	10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Sample_scan_protocol	Affymetrix GeneArray scanner 7G
Sample_data_processing	Feature intensity was extracted and processed using MAS5 by Affymetrix GeneChip Command Console as CEL files. CHP files are generated by Affymetrix Expression Console.
Sample_platform_id	GPL570
Sample_contact_name	Jeffrey,,Zhao
Sample_contact_email	zhaoy@mskcc.org
Sample_contact_department	Genomics Core
Sample_contact_institute	Memorial Sloan Kettering Cancer Center
Sample_contact_address	1275 York Avenue
Sample_contact_city	New York
Sample_contact_state	RRL417A
Sample_contact_zip/postal_code	10065
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631753/suppl/GSM631753.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631753/suppl/GSM631753.CHP.gz
Sample_series_id	GSE25725
Sample_data_row_count	54675

GSM631754

GPL570

FP_sh86_111709

HEL cells with shRNA against PRMT5

genotype/variation: shRNA against PRMT5 cell line: HEL cells

Sample_geo_accession	GSM631754
Sample_status	Public on Jan 01 2011
Sample_submission_date	Nov 30 2010
Sample_last_update_date	Jan 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	0.5 micromolar of cep701 is added to the media for 16 hours. The lentivirus pLKO vector expression PRMT5 shRNA (TRCN0000107086) was used to make stable cell line knocking down PRMt5.
Sample_growth_protocol_ch1	10% fetal bovine serum with RPMI media, 37 degree 5% CO2 incubator
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction plus kit was used to isolate RNA, and cDNA was made with invitrogen superscriptase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
Sample_hyb_protocol	10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Sample_scan_protocol	Affymetrix GeneArray scanner 7G
Sample_data_processing	Feature intensity was extracted and processed using MAS5 by Affymetrix GeneChip Command Console as CEL files. CHP files are generated by Affymetrix Expression Console.
Sample_platform_id	GPL570
Sample_contact_name	Jeffrey,,Zhao
Sample_contact_email	zhaoy@mskcc.org
Sample_contact_department	Genomics Core
Sample_contact_institute	Memorial Sloan Kettering Cancer Center
Sample_contact_address	1275 York Avenue
Sample_contact_city	New York
Sample_contact_state	RRL417A
Sample_contact_zip/postal_code	10065
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631754/suppl/GSM631754.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631754/suppl/GSM631754.CHP.gz
Sample_series_id	GSE25725
Sample_data_row_count	54675

GSM631768

GPL570

FP_DMSO_16hrs_2

DMSO treated HEL cells

treatment: DMSO cell line: HEL cell

Sample_geo_accession	GSM631768
Sample_status	Public on Jan 01 2011
Sample_submission_date	Nov 30 2010
Sample_last_update_date	Jan 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	0.5 micromolar of cep701 is added to the media for 16 hours. The lentivirus pLKO vector expression PRMT5 shRNA (TRCN0000107086) was used to make stable cell line knocking down PRMt5.
Sample_growth_protocol_ch1	10% fetal bovine serum with RPMI media, 37 degree 5% CO2 incubator
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction plus kit was used to isolate RNA, and cDNA was made with invitrogen superscriptase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
Sample_hyb_protocol	10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Sample_scan_protocol	Affymetrix GeneArray scanner 7G
Sample_data_processing	Feature intensity was extracted and processed using MAS5 by Affymetrix GeneChip Command Console as CEL files. CHP files are generated by Affymetrix Expression Console.
Sample_platform_id	GPL570
Sample_contact_name	Jeffrey,,Zhao
Sample_contact_email	zhaoy@mskcc.org
Sample_contact_department	Genomics Core
Sample_contact_institute	Memorial Sloan Kettering Cancer Center
Sample_contact_address	1275 York Avenue
Sample_contact_city	New York
Sample_contact_state	RRL417A
Sample_contact_zip/postal_code	10065
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631768/suppl/GSM631768.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631768/suppl/GSM631768.CHP.gz
Sample_series_id	GSE25725
Sample_data_row_count	54675

GSM631769

GPL570

FP_LKO_scramble_120909

HEL cells with scramble shRNA

genotype/variation: scramble shRNA cell line: HEL cells

Sample_geo_accession	GSM631769
Sample_status	Public on Jan 01 2011
Sample_submission_date	Nov 30 2010
Sample_last_update_date	Jan 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	0.5 micromolar of cep701 is added to the media for 16 hours. The lentivirus pLKO vector expression PRMT5 shRNA (TRCN0000107086) was used to make stable cell line knocking down PRMt5.
Sample_growth_protocol_ch1	10% fetal bovine serum with RPMI media, 37 degree 5% CO2 incubator
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction plus kit was used to isolate RNA, and cDNA was made with invitrogen superscriptase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
Sample_hyb_protocol	10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Sample_scan_protocol	Affymetrix GeneArray scanner 7G
Sample_data_processing	Feature intensity was extracted and processed using MAS5 by Affymetrix GeneChip Command Console as CEL files. CHP files are generated by Affymetrix Expression Console.
Sample_platform_id	GPL570
Sample_contact_name	Jeffrey,,Zhao
Sample_contact_email	zhaoy@mskcc.org
Sample_contact_department	Genomics Core
Sample_contact_institute	Memorial Sloan Kettering Cancer Center
Sample_contact_address	1275 York Avenue
Sample_contact_city	New York
Sample_contact_state	RRL417A
Sample_contact_zip/postal_code	10065
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631769/suppl/GSM631769.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631769/suppl/GSM631769.CHP.gz
Sample_series_id	GSE25725
Sample_data_row_count	54675

GSM631770

GPL570

FP_sh86_120909

HEL cells with shRNA against PRMT5

genotype/variation: shRNA against PRMT5 cell line: HEL cells

Sample_geo_accession	GSM631770
Sample_status	Public on Jan 01 2011
Sample_submission_date	Nov 30 2010
Sample_last_update_date	Jan 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	0.5 micromolar of cep701 is added to the media for 16 hours. The lentivirus pLKO vector expression PRMT5 shRNA (TRCN0000107086) was used to make stable cell line knocking down PRMt5.
Sample_growth_protocol_ch1	10% fetal bovine serum with RPMI media, 37 degree 5% CO2 incubator
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction plus kit was used to isolate RNA, and cDNA was made with invitrogen superscriptase.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	200 ng total RNA converted to cRNA using Affymetrix GeneChip®3' IVT Kit and in vitro transcription resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
Sample_hyb_protocol	10 ug of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Sample_scan_protocol	Affymetrix GeneArray scanner 7G
Sample_data_processing	Feature intensity was extracted and processed using MAS5 by Affymetrix GeneChip Command Console as CEL files. CHP files are generated by Affymetrix Expression Console.
Sample_platform_id	GPL570
Sample_contact_name	Jeffrey,,Zhao
Sample_contact_email	zhaoy@mskcc.org
Sample_contact_department	Genomics Core
Sample_contact_institute	Memorial Sloan Kettering Cancer Center
Sample_contact_address	1275 York Avenue
Sample_contact_city	New York
Sample_contact_state	RRL417A
Sample_contact_zip/postal_code	10065
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631770/suppl/GSM631770.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM631nnn/GSM631770/suppl/GSM631770.CHP.gz
Sample_series_id	GSE25725
Sample_data_row_count	54675

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