Search results for the GEO ID: GSE25743  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM632607 | GPL570 |
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Hs_pi3_LV
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Hs578t cells transfected with pIRESpuro3
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cell line: Hs578T
organ: mammary gland,
disease: breast / carcinoma
atcc number: HTB-126
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Gene expression data from Hs578t cells transfected with pIRESpuro3
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| Sample_geo_accession | GSM632607
| | Sample_status | Public on Dec 02 2010
| | Sample_submission_date | Dec 01 2010
| | Sample_last_update_date | Dec 03 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | 72h, phenol red free MEM medium (PAA) supplemented with 5mM glutamine (Gibco) and 10% fetal bovine serum (FBS superior, Biochrom) at 37°C under 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using TRI-reagent (Molecular Research Centre) and RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer´s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA synthesis and in vitro-transcription: with Ambion´s Message Amp II 96 Kit
| | Sample_hyb_protocol | according to Affymetrix´ GeneChip Expression Analysis Technical Manual. Washing and staining: fluidics station (GeneChip? Fluidics Station450, Affymetrix) controlled by Affymetrix´ software GeneChip Operating System (GCOS) v1.4., Fluidics protocol for eukaryotic 3´-expression arrays: EukGe_WSv4.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix), controlled by Affymetrix´ software GeneChip Operating System (GCOS) v1.4
| | Sample_data_processing | GC Robust Multi-array Average (GCRMA) background adjustment, quantile normalization and median-polish summarization on Affymetrix HGU133 plus 2.0 microarray probe-level data were performed using R 2.7.1 and Bioconductor 2.2. (gcrma package: justGCRMA function). The log2 transformed absolute expression values of the treatment sample (EpCAM positive line) were subtracted from the absolute expression values of the control sample (control line) in a gene-wise manner.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gilbert ,,Spizzo
| | Sample_contact_department | Experimental Oncology
| | Sample_contact_institute | TCRI
| | Sample_contact_address | Innrain 66
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632607/suppl/GSM632607.CEL.gz
| | Sample_series_id | GSE25743
| | Sample_data_row_count | 54675
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GSM632608 | GPL570 |
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Hs_pi3_Ep
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Hs578t cells transfected with pIRESpuro3_EpCAM
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cell line: Hs578T
organ: mammary gland,
disease: breast / carcinoma
atcc number: HTB-126
|
Gene expression data from Hs578t cells transfected with pIRESpuro3_EpCAM
|
| Sample_geo_accession | GSM632608
| | Sample_status | Public on Dec 02 2010
| | Sample_submission_date | Dec 01 2010
| | Sample_last_update_date | Dec 03 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | 72h, phenol red free MEM medium (PAA) supplemented with 5mM glutamine (Gibco) and 10% fetal bovine serum (FBS superior, Biochrom) at 37°C under 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using TRI-reagent (Molecular Research Centre) and RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer´s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA synthesis and in vitro-transcription: with Ambion´s Message Amp II 96 Kit
| | Sample_hyb_protocol | according to Affymetrix´ GeneChip Expression Analysis Technical Manual. Washing and staining: fluidics station (GeneChip? Fluidics Station450, Affymetrix) controlled by Affymetrix´ software GeneChip Operating System (GCOS) v1.4., Fluidics protocol for eukaryotic 3´-expression arrays: EukGe_WSv4.
| | Sample_scan_protocol | GeneChip Scanner 3000 7G (Affymetrix), controlled by Affymetrix´ software GeneChip Operating System (GCOS) v1.4
| | Sample_data_processing | GC Robust Multi-array Average (GCRMA) background adjustment, quantile normalization and median-polish summarization on Affymetrix HGU133 plus 2.0 microarray probe-level data were performed using R 2.7.1 and Bioconductor 2.2. (gcrma package: justGCRMA function). The log2 transformed absolute expression values of the treatment sample (EpCAM positive line) were subtracted from the absolute expression values of the control sample (control line) in a gene-wise manner.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gilbert ,,Spizzo
| | Sample_contact_department | Experimental Oncology
| | Sample_contact_institute | TCRI
| | Sample_contact_address | Innrain 66
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632608/suppl/GSM632608.CEL.gz
| | Sample_series_id | GSE25743
| | Sample_data_row_count | 54675
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