Result

Search results for the GEO ID: GSE25768

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Description

Characteristics

GSM632875

GPL1261

Control heart-age 5 months-BSIX-rep1

Control heart, B6, age 5 months

strain: C57BL/6J genotype/variation: wild type age: 5 months tissue: heart disease status: control

Gene expression data of control B6 heart-age 5 months

Sample_geo_accession	GSM632875
Sample_status	Public on Dec 03 2010
Sample_submission_date	Dec 01 2010
Sample_last_update_date	Dec 02 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study hearts were isolated from four study groups and their respective controls: (i) failing hearts from 18 month-old apoE-deficient mice and age-matched B6 controls; (ii) failing hearts from 6 month-old apoE-deficient mice with 2 months of AAC, and age-matched, sham-operated apoE-deficient and non-transgenic B6 controls, respectively; (iii) failing hearts from 10 month-old B6 mice with 6 months of AAC and age-matched sham-operated B6 controls; (iv) hearts with cardiac hypertrophy isolated from 5 month-old B6 mice with 1 month of AAC and age-matched B6 controls.
Sample_growth_protocol_ch1	The study was performed with 18 month-old apoE-deficient mice on a C57BL/6J (B6) background as a model of atherosclerosis-induced heart failure. Pressure overload-induced heart failure was induced in non-transgenic B6 mice and apoE-deficient mice by abdominal aortic constriction (AAC). All mice had free access to food and water. To induce atherosclerosis-dependent heart failure apoE-deficient mice were fed a rodent chow containing 7 % fat and 0.15 % cholesterol (Ain 93-based diet). All other mice were fed a standard rodent chow containing 4.5 % fat. At an age of 18 months, hearts were isolated from apoE-deficient mice with symptoms of heart failure (ejection fraction ≤33% as determined by echocardiography) and age-matched B6 controls. Failing hearts from aortic constricted mice were isolated from (i) 6 month-old apoE-deficient mice with 2 months of AAC, and from (ii) 10 month-old B6 mice with 6 months of AAC (ejection fraction ≤33 %). As controls, heart tissue from age-matched, sham-operated apoE-deficient and non-transgenic B6 mice were used. Hearts from 5 month-old B6 mice with 1 month of AAC were also included in the study displaying cardiac hypertrophy without failure (ejection fraction ≥50 %).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632875/suppl/GSM632875.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632875/suppl/GSM632875.CHP.gz
Sample_series_id	GSE25765
Sample_series_id	GSE25768
Sample_data_row_count	45101

GSM632876

GPL1261

Control heart-age 5 months-BSIX-rep2

Control heart, B6, age 5 months

strain: C57BL/6J genotype/variation: wild type age: 5 months tissue: heart disease status: control

Gene expression data of control B6 heart-age 5 months

Sample_geo_accession	GSM632876
Sample_status	Public on Dec 03 2010
Sample_submission_date	Dec 01 2010
Sample_last_update_date	Dec 02 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study hearts were isolated from four study groups and their respective controls: (i) failing hearts from 18 month-old apoE-deficient mice and age-matched B6 controls; (ii) failing hearts from 6 month-old apoE-deficient mice with 2 months of AAC, and age-matched, sham-operated apoE-deficient and non-transgenic B6 controls, respectively; (iii) failing hearts from 10 month-old B6 mice with 6 months of AAC and age-matched sham-operated B6 controls; (iv) hearts with cardiac hypertrophy isolated from 5 month-old B6 mice with 1 month of AAC and age-matched B6 controls.
Sample_growth_protocol_ch1	The study was performed with 18 month-old apoE-deficient mice on a C57BL/6J (B6) background as a model of atherosclerosis-induced heart failure. Pressure overload-induced heart failure was induced in non-transgenic B6 mice and apoE-deficient mice by abdominal aortic constriction (AAC). All mice had free access to food and water. To induce atherosclerosis-dependent heart failure apoE-deficient mice were fed a rodent chow containing 7 % fat and 0.15 % cholesterol (Ain 93-based diet). All other mice were fed a standard rodent chow containing 4.5 % fat. At an age of 18 months, hearts were isolated from apoE-deficient mice with symptoms of heart failure (ejection fraction ≤33% as determined by echocardiography) and age-matched B6 controls. Failing hearts from aortic constricted mice were isolated from (i) 6 month-old apoE-deficient mice with 2 months of AAC, and from (ii) 10 month-old B6 mice with 6 months of AAC (ejection fraction ≤33 %). As controls, heart tissue from age-matched, sham-operated apoE-deficient and non-transgenic B6 mice were used. Hearts from 5 month-old B6 mice with 1 month of AAC were also included in the study displaying cardiac hypertrophy without failure (ejection fraction ≥50 %).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632876/suppl/GSM632876.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632876/suppl/GSM632876.CHP.gz
Sample_series_id	GSE25765
Sample_series_id	GSE25768
Sample_data_row_count	45101

GSM632881

GPL1261

Failing heart-apoE-deficient-1 month of cystamine-age 5 months-rep1

Failing heart, apoE-deficient, 1 month of cystamine, age 5 months

strain: C57BL/6J genotype/variation: apoE-deficient age: 5 months tissue: heart disease status: failing treatment: 1 month of cystamine

Gene expression data of failing apoE-deficient heart with 1 month of cystamine-age 5 months

Sample_geo_accession	GSM632881
Sample_status	Public on Dec 03 2010
Sample_submission_date	Dec 01 2010
Sample_last_update_date	Dec 02 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study hearts were isolated from three study groups: (i) failing hearts from 5 month-old apoE-deficient mice with 1 month of cystamine treatment (300 mg/kg in drinking water), (ii) hearts from 5 month-old untreated apoE-deficient mice, and (iii) hearts from 5 month-old untreated B6 controls.
Sample_growth_protocol_ch1	The study was performed with 5 month-old apolipoprotein E- (apoE)- deficient mice treated for 1 month with the mild thiol-blocking agent cystamine to inhibit cellular respiration. Control groups consisted of age-matched untreated apoE-deficient mice and non-transgenic B6 mice. All mice had free access to food and water. Mice were fed a rodent chow containing 7 % fat and 0.15 % cholesterol (Ain 93-based diet). At an age of 4 months, the treatment group of apoE-deficient mice received cystamine (300 mg/kg in drinking water) for 1 month. At an age of 5 months, cardiac function of all study groups was determined by echocardiography and hearts were isolated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632881/suppl/GSM632881.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632881/suppl/GSM632881.CHP.gz
Sample_series_id	GSE25768
Sample_data_row_count	45101

GSM632882

GPL1261

Failing heart-apoE-deficient-1 month of cystamine-age 5 months-rep2

Failing heart, apoE-deficient, 1 month of cystamine, age 5 months

strain: C57BL/6J genotype/variation: apoE-deficient age: 5 months tissue: heart disease status: failing treatment: 1 month of cystamine

Gene expression data of failing apoE-deficient heart with 1 month of cystamine-age 5 months

Sample_geo_accession	GSM632882
Sample_status	Public on Dec 03 2010
Sample_submission_date	Dec 01 2010
Sample_last_update_date	Dec 02 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study hearts were isolated from three study groups: (i) failing hearts from 5 month-old apoE-deficient mice with 1 month of cystamine treatment (300 mg/kg in drinking water), (ii) hearts from 5 month-old untreated apoE-deficient mice, and (iii) hearts from 5 month-old untreated B6 controls.
Sample_growth_protocol_ch1	The study was performed with 5 month-old apolipoprotein E- (apoE)- deficient mice treated for 1 month with the mild thiol-blocking agent cystamine to inhibit cellular respiration. Control groups consisted of age-matched untreated apoE-deficient mice and non-transgenic B6 mice. All mice had free access to food and water. Mice were fed a rodent chow containing 7 % fat and 0.15 % cholesterol (Ain 93-based diet). At an age of 4 months, the treatment group of apoE-deficient mice received cystamine (300 mg/kg in drinking water) for 1 month. At an age of 5 months, cardiac function of all study groups was determined by echocardiography and hearts were isolated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632882/suppl/GSM632882.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632882/suppl/GSM632882.CHP.gz
Sample_series_id	GSE25768
Sample_data_row_count	45101

GSM632883

GPL1261

Heart-apoE-deficient-age 5 months-rep1

Heart, apoE-deficient, age 5 months

strain: C57BL/6J genotype/variation: apoE-deficient age: 5 months tissue: heart disease status: control treatment: untreated

Gene expression data of apoE-deficient heart, age 5 months

Sample_geo_accession	GSM632883
Sample_status	Public on Dec 03 2010
Sample_submission_date	Dec 01 2010
Sample_last_update_date	Dec 02 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study hearts were isolated from three study groups: (i) failing hearts from 5 month-old apoE-deficient mice with 1 month of cystamine treatment (300 mg/kg in drinking water), (ii) hearts from 5 month-old untreated apoE-deficient mice, and (iii) hearts from 5 month-old untreated B6 controls.
Sample_growth_protocol_ch1	The study was performed with 5 month-old apolipoprotein E- (apoE)- deficient mice treated for 1 month with the mild thiol-blocking agent cystamine to inhibit cellular respiration. Control groups consisted of age-matched untreated apoE-deficient mice and non-transgenic B6 mice. All mice had free access to food and water. Mice were fed a rodent chow containing 7 % fat and 0.15 % cholesterol (Ain 93-based diet). At an age of 4 months, the treatment group of apoE-deficient mice received cystamine (300 mg/kg in drinking water) for 1 month. At an age of 5 months, cardiac function of all study groups was determined by echocardiography and hearts were isolated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632883/suppl/GSM632883.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632883/suppl/GSM632883.CHP.gz
Sample_series_id	GSE25768
Sample_data_row_count	45101

GSM632884

GPL1261

Heart-apoE-deficient-age 5 months-rep2

Heart, apoE-deficient, age 5 months

strain: C57BL/6J genotype/variation: apoE-deficient age: 5 months tissue: heart disease status: control treatment: untreated

Gene expression data of apoE-deficient heart, age 5 months

Sample_geo_accession	GSM632884
Sample_status	Public on Dec 03 2010
Sample_submission_date	Dec 01 2010
Sample_last_update_date	Dec 02 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study hearts were isolated from three study groups: (i) failing hearts from 5 month-old apoE-deficient mice with 1 month of cystamine treatment (300 mg/kg in drinking water), (ii) hearts from 5 month-old untreated apoE-deficient mice, and (iii) hearts from 5 month-old untreated B6 controls.
Sample_growth_protocol_ch1	The study was performed with 5 month-old apolipoprotein E- (apoE)- deficient mice treated for 1 month with the mild thiol-blocking agent cystamine to inhibit cellular respiration. Control groups consisted of age-matched untreated apoE-deficient mice and non-transgenic B6 mice. All mice had free access to food and water. Mice were fed a rodent chow containing 7 % fat and 0.15 % cholesterol (Ain 93-based diet). At an age of 4 months, the treatment group of apoE-deficient mice received cystamine (300 mg/kg in drinking water) for 1 month. At an age of 5 months, cardiac function of all study groups was determined by echocardiography and hearts were isolated.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632884/suppl/GSM632884.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM632nnn/GSM632884/suppl/GSM632884.CHP.gz
Sample_series_id	GSE25768
Sample_data_row_count	45101

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