Search results for the GEO ID: GSE25827  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM634491 | GPL1261 |
|
C2-0day-rep1
|
C2C12 cells in GM
|
cell line: C2C12
medium: growth
infection: none
time: 0 days
|
C2C12 cells grown in GM.
|
| Sample_geo_accession | GSM634491
| | Sample_status | Public on Feb 21 2011
| | Sample_submission_date | Dec 02 2010
| | Sample_last_update_date | Feb 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | C2C12 cells were infected with adenovirus containing lacZ or dnMEK5 1 day after differentiation. For each experiment, five samples were used: cells in growth medium (0 day), lacZ-infected cells at two time points (lacZ- 2.5 day and lacZ- 4.5 day) and dnMEK5-infected cells at two time points (dnMEK5- 2.5 day and dnMEK5- 4.5 day).
| | Sample_growth_protocol_ch1 | C2C12 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 15% fetal bovine serum (FBS) (growth medium; GM). To induce differentiation, growth medium was replaced with DMEM containing 3% horse serum (differentiation medium; DM) when cells were 90-100% confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol (One-Cycle Target Labeling Assays) from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Styation 450.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with the GCRMA algorithm (GeneSpring GX 11.0.2).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eisuke,,Nishida
| | Sample_contact_email | nishida@lif.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-753-4230
| | Sample_contact_fax | +81-75-753-4235
| | Sample_contact_department | Department of Cell and Developmental Biology
| | Sample_contact_institute | Graduate School of Biostudies, Kyoto University
| | Sample_contact_address | Kitashirakawa, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8502
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM634nnn/GSM634491/suppl/GSM634491.CEL.gz
| | Sample_series_id | GSE25827
| | Sample_data_row_count | 45101
| | |
|
GSM634492 | GPL1261 |
|
C2-lacZ-2.5day-rep1
|
lacZ-infected C2C12 cells in DM
|
cell line: C2C12
medium: differentiation
infection: lacZ
time: 2.5 days
|
C2C12 cells were placed in DM and infected with adenovirus containing lacZ 1 day of differentiation. Cells were collected at 2.5 days of differentiation.
|
| Sample_geo_accession | GSM634492
| | Sample_status | Public on Feb 21 2011
| | Sample_submission_date | Dec 02 2010
| | Sample_last_update_date | Feb 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | C2C12 cells were infected with adenovirus containing lacZ or dnMEK5 1 day after differentiation. For each experiment, five samples were used: cells in growth medium (0 day), lacZ-infected cells at two time points (lacZ- 2.5 day and lacZ- 4.5 day) and dnMEK5-infected cells at two time points (dnMEK5- 2.5 day and dnMEK5- 4.5 day).
| | Sample_growth_protocol_ch1 | C2C12 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 15% fetal bovine serum (FBS) (growth medium; GM). To induce differentiation, growth medium was replaced with DMEM containing 3% horse serum (differentiation medium; DM) when cells were 90-100% confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol (One-Cycle Target Labeling Assays) from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Styation 450.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with the GCRMA algorithm (GeneSpring GX 11.0.2).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eisuke,,Nishida
| | Sample_contact_email | nishida@lif.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-753-4230
| | Sample_contact_fax | +81-75-753-4235
| | Sample_contact_department | Department of Cell and Developmental Biology
| | Sample_contact_institute | Graduate School of Biostudies, Kyoto University
| | Sample_contact_address | Kitashirakawa, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8502
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM634nnn/GSM634492/suppl/GSM634492.CEL.gz
| | Sample_series_id | GSE25827
| | Sample_data_row_count | 45101
| | |
|
GSM634493 | GPL1261 |
|
C2-lacZ-4.5day-rep1
|
lacZ-infected C2C12 cells in DM
|
cell line: C2C12
medium: differentiation
infection: lacZ
time: 4 .5 days
|
C2C12 cells were placed in DM and infected with adenovirus containing lacZ 1 day of differentiation. Cells were collected at 4.5 days of differentiation.
|
| Sample_geo_accession | GSM634493
| | Sample_status | Public on Feb 21 2011
| | Sample_submission_date | Dec 02 2010
| | Sample_last_update_date | Feb 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | C2C12 cells were infected with adenovirus containing lacZ or dnMEK5 1 day after differentiation. For each experiment, five samples were used: cells in growth medium (0 day), lacZ-infected cells at two time points (lacZ- 2.5 day and lacZ- 4.5 day) and dnMEK5-infected cells at two time points (dnMEK5- 2.5 day and dnMEK5- 4.5 day).
| | Sample_growth_protocol_ch1 | C2C12 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 15% fetal bovine serum (FBS) (growth medium; GM). To induce differentiation, growth medium was replaced with DMEM containing 3% horse serum (differentiation medium; DM) when cells were 90-100% confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol (One-Cycle Target Labeling Assays) from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Styation 450.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with the GCRMA algorithm (GeneSpring GX 11.0.2).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eisuke,,Nishida
| | Sample_contact_email | nishida@lif.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-753-4230
| | Sample_contact_fax | +81-75-753-4235
| | Sample_contact_department | Department of Cell and Developmental Biology
| | Sample_contact_institute | Graduate School of Biostudies, Kyoto University
| | Sample_contact_address | Kitashirakawa, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8502
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM634nnn/GSM634493/suppl/GSM634493.CEL.gz
| | Sample_series_id | GSE25827
| | Sample_data_row_count | 45101
| | |
|
GSM634494 | GPL1261 |
|
C2-dnMEK5-2.5day-rep1
|
dnMEK5-infected C2C12 cells in DM
|
cell line: C2C12
medium: differentiation
infection: dnMEK5
time: 2.5 days
|
C2C12 cells were placed in DM and infected with adenovirus containing dnMEK5 1 day of differentiation. Cells were collected at 2.5 days of differentiation.
|
| Sample_geo_accession | GSM634494
| | Sample_status | Public on Feb 21 2011
| | Sample_submission_date | Dec 02 2010
| | Sample_last_update_date | Feb 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | C2C12 cells were infected with adenovirus containing lacZ or dnMEK5 1 day after differentiation. For each experiment, five samples were used: cells in growth medium (0 day), lacZ-infected cells at two time points (lacZ- 2.5 day and lacZ- 4.5 day) and dnMEK5-infected cells at two time points (dnMEK5- 2.5 day and dnMEK5- 4.5 day).
| | Sample_growth_protocol_ch1 | C2C12 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 15% fetal bovine serum (FBS) (growth medium; GM). To induce differentiation, growth medium was replaced with DMEM containing 3% horse serum (differentiation medium; DM) when cells were 90-100% confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol (One-Cycle Target Labeling Assays) from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Styation 450.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with the GCRMA algorithm (GeneSpring GX 11.0.2).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eisuke,,Nishida
| | Sample_contact_email | nishida@lif.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-753-4230
| | Sample_contact_fax | +81-75-753-4235
| | Sample_contact_department | Department of Cell and Developmental Biology
| | Sample_contact_institute | Graduate School of Biostudies, Kyoto University
| | Sample_contact_address | Kitashirakawa, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8502
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM634nnn/GSM634494/suppl/GSM634494.CEL.gz
| | Sample_series_id | GSE25827
| | Sample_data_row_count | 45101
| | |
|
GSM634495 | GPL1261 |
|
C2-dnMEK5-4.5day-rep1
|
dnMEK5-infected C2C12 cells in DM
|
cell line: C2C12
medium: differentiation
infection: dnMEK5
time: 4 .5 days
|
C2C12 cells were placed in DM and infected with adenovirus containing dnMEK5 1 day of differentiation. Cells were collected at 4.5 days of differentiation.
|
| Sample_geo_accession | GSM634495
| | Sample_status | Public on Feb 21 2011
| | Sample_submission_date | Dec 02 2010
| | Sample_last_update_date | Feb 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | C2C12 cells were infected with adenovirus containing lacZ or dnMEK5 1 day after differentiation. For each experiment, five samples were used: cells in growth medium (0 day), lacZ-infected cells at two time points (lacZ- 2.5 day and lacZ- 4.5 day) and dnMEK5-infected cells at two time points (dnMEK5- 2.5 day and dnMEK5- 4.5 day).
| | Sample_growth_protocol_ch1 | C2C12 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 15% fetal bovine serum (FBS) (growth medium; GM). To induce differentiation, growth medium was replaced with DMEM containing 3% horse serum (differentiation medium; DM) when cells were 90-100% confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol (One-Cycle Target Labeling Assays) from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Styation 450.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with the GCRMA algorithm (GeneSpring GX 11.0.2).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eisuke,,Nishida
| | Sample_contact_email | nishida@lif.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-753-4230
| | Sample_contact_fax | +81-75-753-4235
| | Sample_contact_department | Department of Cell and Developmental Biology
| | Sample_contact_institute | Graduate School of Biostudies, Kyoto University
| | Sample_contact_address | Kitashirakawa, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8502
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM634nnn/GSM634495/suppl/GSM634495.CEL.gz
| | Sample_series_id | GSE25827
| | Sample_data_row_count | 45101
| | |
|
GSM634496 | GPL1261 |
|
C2-0day-rep2
|
C2C12 cells in GM
|
cell line: C2C12
medium: growth
infection: none
time: 0 days
|
C2C12 cells grown in GM.
|
| Sample_geo_accession | GSM634496
| | Sample_status | Public on Feb 21 2011
| | Sample_submission_date | Dec 02 2010
| | Sample_last_update_date | Feb 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | C2C12 cells were infected with adenovirus containing lacZ or dnMEK5 1 day after differentiation. For each experiment, five samples were used: cells in growth medium (0 day), lacZ-infected cells at two time points (lacZ- 2.5 day and lacZ- 4.5 day) and dnMEK5-infected cells at two time points (dnMEK5- 2.5 day and dnMEK5- 4.5 day).
| | Sample_growth_protocol_ch1 | C2C12 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 15% fetal bovine serum (FBS) (growth medium; GM). To induce differentiation, growth medium was replaced with DMEM containing 3% horse serum (differentiation medium; DM) when cells were 90-100% confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol (One-Cycle Target Labeling Assays) from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Styation 450.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with the GCRMA algorithm (GeneSpring GX 11.0.2).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eisuke,,Nishida
| | Sample_contact_email | nishida@lif.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-753-4230
| | Sample_contact_fax | +81-75-753-4235
| | Sample_contact_department | Department of Cell and Developmental Biology
| | Sample_contact_institute | Graduate School of Biostudies, Kyoto University
| | Sample_contact_address | Kitashirakawa, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8502
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM634nnn/GSM634496/suppl/GSM634496.CEL.gz
| | Sample_series_id | GSE25827
| | Sample_data_row_count | 45101
| | |
|
GSM634497 | GPL1261 |
|
C2-lacZ-2.5day-rep2
|
lacZ-infected C2C12 cells in DM
|
cell line: C2C12
medium: differentiation
infection: lacZ
time: 2.5 days
|
C2C12 cells were placed in DM and infected with adenovirus containing lacZ 1 day of differentiation. Cells were collected at 2.5 days of differentiation.
|
| Sample_geo_accession | GSM634497
| | Sample_status | Public on Feb 21 2011
| | Sample_submission_date | Dec 02 2010
| | Sample_last_update_date | Feb 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | C2C12 cells were infected with adenovirus containing lacZ or dnMEK5 1 day after differentiation. For each experiment, five samples were used: cells in growth medium (0 day), lacZ-infected cells at two time points (lacZ- 2.5 day and lacZ- 4.5 day) and dnMEK5-infected cells at two time points (dnMEK5- 2.5 day and dnMEK5- 4.5 day).
| | Sample_growth_protocol_ch1 | C2C12 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 15% fetal bovine serum (FBS) (growth medium; GM). To induce differentiation, growth medium was replaced with DMEM containing 3% horse serum (differentiation medium; DM) when cells were 90-100% confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol (One-Cycle Target Labeling Assays) from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Styation 450.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with the GCRMA algorithm (GeneSpring GX 11.0.2).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eisuke,,Nishida
| | Sample_contact_email | nishida@lif.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-753-4230
| | Sample_contact_fax | +81-75-753-4235
| | Sample_contact_department | Department of Cell and Developmental Biology
| | Sample_contact_institute | Graduate School of Biostudies, Kyoto University
| | Sample_contact_address | Kitashirakawa, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8502
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM634nnn/GSM634497/suppl/GSM634497.CEL.gz
| | Sample_series_id | GSE25827
| | Sample_data_row_count | 45101
| | |
|
GSM634498 | GPL1261 |
|
C2-lacZ-4.5day-rep2
|
lacZ-infected C2C12 cells in DM
|
cell line: C2C12
medium: differentiation
infection: lacZ
time: 4 .5 days
|
C2C12 cells were placed in DM and infected with adenovirus containing lacZ 1 day of differentiation. Cells were collected at 4.5 days of differentiation.
|
| Sample_geo_accession | GSM634498
| | Sample_status | Public on Feb 21 2011
| | Sample_submission_date | Dec 02 2010
| | Sample_last_update_date | Feb 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | C2C12 cells were infected with adenovirus containing lacZ or dnMEK5 1 day after differentiation. For each experiment, five samples were used: cells in growth medium (0 day), lacZ-infected cells at two time points (lacZ- 2.5 day and lacZ- 4.5 day) and dnMEK5-infected cells at two time points (dnMEK5- 2.5 day and dnMEK5- 4.5 day).
| | Sample_growth_protocol_ch1 | C2C12 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 15% fetal bovine serum (FBS) (growth medium; GM). To induce differentiation, growth medium was replaced with DMEM containing 3% horse serum (differentiation medium; DM) when cells were 90-100% confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol (One-Cycle Target Labeling Assays) from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Styation 450.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with the GCRMA algorithm (GeneSpring GX 11.0.2).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eisuke,,Nishida
| | Sample_contact_email | nishida@lif.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-753-4230
| | Sample_contact_fax | +81-75-753-4235
| | Sample_contact_department | Department of Cell and Developmental Biology
| | Sample_contact_institute | Graduate School of Biostudies, Kyoto University
| | Sample_contact_address | Kitashirakawa, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8502
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM634nnn/GSM634498/suppl/GSM634498.CEL.gz
| | Sample_series_id | GSE25827
| | Sample_data_row_count | 45101
| | |
|
GSM634499 | GPL1261 |
|
C2-dnMEK5-2.5day-rep2
|
dnMEK5-infected C2C12 cells in DM
|
cell line: C2C12
medium: differentiation
infection: dnMEK5
time: 2.5 days
|
C2C12 cells were placed in DM and infected with adenovirus containing dnMEK5 1 day of differentiation. Cells were collected at 2.5 days of differentiation.
|
| Sample_geo_accession | GSM634499
| | Sample_status | Public on Feb 21 2011
| | Sample_submission_date | Dec 02 2010
| | Sample_last_update_date | Feb 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | C2C12 cells were infected with adenovirus containing lacZ or dnMEK5 1 day after differentiation. For each experiment, five samples were used: cells in growth medium (0 day), lacZ-infected cells at two time points (lacZ- 2.5 day and lacZ- 4.5 day) and dnMEK5-infected cells at two time points (dnMEK5- 2.5 day and dnMEK5- 4.5 day).
| | Sample_growth_protocol_ch1 | C2C12 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 15% fetal bovine serum (FBS) (growth medium; GM). To induce differentiation, growth medium was replaced with DMEM containing 3% horse serum (differentiation medium; DM) when cells were 90-100% confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol (One-Cycle Target Labeling Assays) from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Styation 450.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with the GCRMA algorithm (GeneSpring GX 11.0.2).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eisuke,,Nishida
| | Sample_contact_email | nishida@lif.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-753-4230
| | Sample_contact_fax | +81-75-753-4235
| | Sample_contact_department | Department of Cell and Developmental Biology
| | Sample_contact_institute | Graduate School of Biostudies, Kyoto University
| | Sample_contact_address | Kitashirakawa, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8502
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM634nnn/GSM634499/suppl/GSM634499.CEL.gz
| | Sample_series_id | GSE25827
| | Sample_data_row_count | 45101
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GSM634500 | GPL1261 |
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C2-dnMEK5-4.5day-rep2
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dnMEK5-infected C2C12 cells in DM
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cell line: C2C12
medium: differentiation
infection: dnMEK5
time: 4 .5 days
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C2C12 cells were placed in DM and infected with adenovirus containing dnMEK5 1 day of differentiation. Cells were collected at 4.5 days of differentiation.
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| Sample_geo_accession | GSM634500
| | Sample_status | Public on Feb 21 2011
| | Sample_submission_date | Dec 02 2010
| | Sample_last_update_date | Feb 21 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | C2C12 cells were infected with adenovirus containing lacZ or dnMEK5 1 day after differentiation. For each experiment, five samples were used: cells in growth medium (0 day), lacZ-infected cells at two time points (lacZ- 2.5 day and lacZ- 4.5 day) and dnMEK5-infected cells at two time points (dnMEK5- 2.5 day and dnMEK5- 4.5 day).
| | Sample_growth_protocol_ch1 | C2C12 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 15% fetal bovine serum (FBS) (growth medium; GM). To induce differentiation, growth medium was replaced with DMEM containing 3% horse serum (differentiation medium; DM) when cells were 90-100% confluent.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol (One-Cycle Target Labeling Assays) from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees C on Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Styation 450.
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with the GCRMA algorithm (GeneSpring GX 11.0.2).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eisuke,,Nishida
| | Sample_contact_email | nishida@lif.kyoto-u.ac.jp
| | Sample_contact_phone | +81-75-753-4230
| | Sample_contact_fax | +81-75-753-4235
| | Sample_contact_department | Department of Cell and Developmental Biology
| | Sample_contact_institute | Graduate School of Biostudies, Kyoto University
| | Sample_contact_address | Kitashirakawa, Sakyo-ku
| | Sample_contact_city | Kyoto
| | Sample_contact_zip/postal_code | 606-8502
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM634nnn/GSM634500/suppl/GSM634500.CEL.gz
| | Sample_series_id | GSE25827
| | Sample_data_row_count | 45101
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