Search results for the GEO ID: GSE25890 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM635583 | GPL1261 |
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Ex vivo nuocytes day 0 rep1
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IL-25-induced nuocytes
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cell type: Splenic nuocytes
time: 0 days in culture
trreatment: 3 daily administrations of IL-25.
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Gene expression data from nuocytes directly isolated from the spleen following 3 daily administrations of IL-25.
|
Sample_geo_accession | GSM635583
| Sample_status | Public on Dec 09 2010
| Sample_submission_date | Dec 07 2010
| Sample_last_update_date | Dec 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Nuocytes were purified by flow cytometry from the spleens of mice treated with 3 daily doses of IL-25
| Sample_growth_protocol_ch1 | Nuocytes were purified directly from spleen and prepared for microarray or cultured with IL-33 and IL-7 (both at 10 nanograms/ml) for 9 days before being prepared for microarray gene expression analysis
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted with the Qiagen RNAeasy Mini Kit according to the manufacturer's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labeling was done using NuGEN Encore Biotin Module according to manufacturer’s protocol
| Sample_hyb_protocol | Hybridization were done on Affymetrix GeneChips (Mouse Genome 430 2.0 cartridge arrays) for 16 hours, 48C and 55rpm, then washed on an Affymetrix FS450 using the EukGE-WS2v4_450 protocol instrument
| Sample_scan_protocol | Arrays were scanned using an Affymetrix GCS3000 7G system
| Sample_data_processing | RMA normalization of the data followed by two-way ANOVAs using the default analysis settings in Array Studio
| Sample_platform_id | GPL1261
| Sample_contact_name | Andrew,,MCKENZIE
| Sample_contact_email | anm@mrc-lmb.cam.ac.uk
| Sample_contact_phone | 44 1223 402377
| Sample_contact_institute | MRC-LMB
| Sample_contact_address | HILLS ROAD
| Sample_contact_city | CAMBRIDGE
| Sample_contact_zip/postal_code | CB2 0QH
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM635nnn/GSM635583/suppl/GSM635583_Day_0_Nuocyte1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM635nnn/GSM635583/suppl/GSM635583_Day_0_Nuocyte1.rma.chp.gz
| Sample_series_id | GSE25890
| Sample_data_row_count | 45101
| |
|
GSM635584 | GPL1261 |
|
Ex vivo nuocytes day 0 rep2
|
IL-25-induced nuocytes
|
cell type: Splenic nuocytes
time: 0 days in culture
trreatment: 3 daily administrations of IL-25.
|
Gene expression data from nuocytes directly isolated from the spleen following 3 daily administrations of IL-25.
|
Sample_geo_accession | GSM635584
| Sample_status | Public on Dec 09 2010
| Sample_submission_date | Dec 07 2010
| Sample_last_update_date | Dec 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Nuocytes were purified by flow cytometry from the spleens of mice treated with 3 daily doses of IL-25
| Sample_growth_protocol_ch1 | Nuocytes were purified directly from spleen and prepared for microarray or cultured with IL-33 and IL-7 (both at 10 nanograms/ml) for 9 days before being prepared for microarray gene expression analysis
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted with the Qiagen RNAeasy Mini Kit according to the manufacturer's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labeling was done using NuGEN Encore Biotin Module according to manufacturer’s protocol
| Sample_hyb_protocol | Hybridization were done on Affymetrix GeneChips (Mouse Genome 430 2.0 cartridge arrays) for 16 hours, 48C and 55rpm, then washed on an Affymetrix FS450 using the EukGE-WS2v4_450 protocol instrument
| Sample_scan_protocol | Arrays were scanned using an Affymetrix GCS3000 7G system
| Sample_data_processing | RMA normalization of the data followed by two-way ANOVAs using the default analysis settings in Array Studio
| Sample_platform_id | GPL1261
| Sample_contact_name | Andrew,,MCKENZIE
| Sample_contact_email | anm@mrc-lmb.cam.ac.uk
| Sample_contact_phone | 44 1223 402377
| Sample_contact_institute | MRC-LMB
| Sample_contact_address | HILLS ROAD
| Sample_contact_city | CAMBRIDGE
| Sample_contact_zip/postal_code | CB2 0QH
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM635nnn/GSM635584/suppl/GSM635584_Day_0_nuocyte2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM635nnn/GSM635584/suppl/GSM635584_Day_0_nuocyte2.rma.chp.gz
| Sample_series_id | GSE25890
| Sample_data_row_count | 45101
| |
|
GSM635585 | GPL1261 |
|
Nuocytes cultured for 9 days rep1
|
IL-25-induced nuocytes cultured with IL-33 and IL-7 for 9 days
|
cell type: Splenic nuocytes
time: 9 days in culture
trreatment: 3 daily administrations of IL-25 and cultured with IL-33 and IL-7 (both at 10 nanograms /ml) for 9 days.
|
Gene expression data from nuocytes isolated from the spleen following 3 daily administrations of IL-25 and cultured with IL-33 and IL-7 (both at 10 nanograms /ml) for 9 days.
|
Sample_geo_accession | GSM635585
| Sample_status | Public on Dec 09 2010
| Sample_submission_date | Dec 07 2010
| Sample_last_update_date | Dec 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Nuocytes were purified by flow cytometry from the spleens of mice treated with 3 daily doses of IL-25
| Sample_growth_protocol_ch1 | Nuocytes were purified directly from spleen and prepared for microarray or cultured with IL-33 and IL-7 (both at 10 nanograms/ml) for 9 days before being prepared for microarray gene expression analysis
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted with the Qiagen RNAeasy Mini Kit according to the manufacturer's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labeling was done using NuGEN Encore Biotin Module according to manufacturer’s protocol
| Sample_hyb_protocol | Hybridization were done on Affymetrix GeneChips (Mouse Genome 430 2.0 cartridge arrays) for 16 hours, 48C and 55rpm, then washed on an Affymetrix FS450 using the EukGE-WS2v4_450 protocol instrument
| Sample_scan_protocol | Arrays were scanned using an Affymetrix GCS3000 7G system
| Sample_data_processing | RMA normalization of the data followed by two-way ANOVAs using the default analysis settings in Array Studio
| Sample_platform_id | GPL1261
| Sample_contact_name | Andrew,,MCKENZIE
| Sample_contact_email | anm@mrc-lmb.cam.ac.uk
| Sample_contact_phone | 44 1223 402377
| Sample_contact_institute | MRC-LMB
| Sample_contact_address | HILLS ROAD
| Sample_contact_city | CAMBRIDGE
| Sample_contact_zip/postal_code | CB2 0QH
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM635nnn/GSM635585/suppl/GSM635585_Day_9_Nuocyte1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM635nnn/GSM635585/suppl/GSM635585_Day_9_Nuocyte1.rma.chp.gz
| Sample_series_id | GSE25890
| Sample_data_row_count | 45101
| |
|
GSM635586 | GPL1261 |
|
Nuocytes cultured for 9 days rep2
|
IL-25-induced nuocytes cultured with IL-33 and IL-7 for 9 days
|
cell type: Splenic nuocytes
time: 9 days in culture
trreatment: 3 daily administrations of IL-25 and cultured with IL-33 and IL-7 (both at 10 nanograms /ml) for 9 days.
|
Gene expression data from nuocytes isolated from the spleen following 3 daily administrations of IL-25 and cultured with IL-33 and IL-7 (both at 10 nanograms /ml) for 9 days.
|
Sample_geo_accession | GSM635586
| Sample_status | Public on Dec 09 2010
| Sample_submission_date | Dec 07 2010
| Sample_last_update_date | Dec 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Nuocytes were purified by flow cytometry from the spleens of mice treated with 3 daily doses of IL-25
| Sample_growth_protocol_ch1 | Nuocytes were purified directly from spleen and prepared for microarray or cultured with IL-33 and IL-7 (both at 10 nanograms/ml) for 9 days before being prepared for microarray gene expression analysis
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted with the Qiagen RNAeasy Mini Kit according to the manufacturer's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labeling was done using NuGEN Encore Biotin Module according to manufacturer’s protocol
| Sample_hyb_protocol | Hybridization were done on Affymetrix GeneChips (Mouse Genome 430 2.0 cartridge arrays) for 16 hours, 48C and 55rpm, then washed on an Affymetrix FS450 using the EukGE-WS2v4_450 protocol instrument
| Sample_scan_protocol | Arrays were scanned using an Affymetrix GCS3000 7G system
| Sample_data_processing | RMA normalization of the data followed by two-way ANOVAs using the default analysis settings in Array Studio
| Sample_platform_id | GPL1261
| Sample_contact_name | Andrew,,MCKENZIE
| Sample_contact_email | anm@mrc-lmb.cam.ac.uk
| Sample_contact_phone | 44 1223 402377
| Sample_contact_institute | MRC-LMB
| Sample_contact_address | HILLS ROAD
| Sample_contact_city | CAMBRIDGE
| Sample_contact_zip/postal_code | CB2 0QH
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM635nnn/GSM635586/suppl/GSM635586_Day_9_Nuocyte2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM635nnn/GSM635586/suppl/GSM635586_Day_9_Nuocyte2.rma.chp.gz
| Sample_series_id | GSE25890
| Sample_data_row_count | 45101
| |
|
GSM635587 | GPL1261 |
|
Nuocytes cultured for 9 days rep3
|
IL-25-induced nuocytes cultured with IL-33 and IL-7 for 9 days
|
cell type: Splenic nuocytes
time: 9 days in culture
trreatment: 3 daily administrations of IL-25 and cultured with IL-33 and IL-7 (both at 10 nanograms /ml) for 9 days.
|
Gene expression data from nuocytes isolated from the spleen following 3 daily administrations of IL-25 and cultured with IL-33 and IL-7 (both at 10 nanograms /ml) for 9 days.
|
Sample_geo_accession | GSM635587
| Sample_status | Public on Dec 09 2010
| Sample_submission_date | Dec 07 2010
| Sample_last_update_date | Dec 09 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Nuocytes were purified by flow cytometry from the spleens of mice treated with 3 daily doses of IL-25
| Sample_growth_protocol_ch1 | Nuocytes were purified directly from spleen and prepared for microarray or cultured with IL-33 and IL-7 (both at 10 nanograms/ml) for 9 days before being prepared for microarray gene expression analysis
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted with the Qiagen RNAeasy Mini Kit according to the manufacturer's protocol.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labeling was done using NuGEN Encore Biotin Module according to manufacturer’s protocol
| Sample_hyb_protocol | Hybridization were done on Affymetrix GeneChips (Mouse Genome 430 2.0 cartridge arrays) for 16 hours, 48C and 55rpm, then washed on an Affymetrix FS450 using the EukGE-WS2v4_450 protocol instrument
| Sample_scan_protocol | Arrays were scanned using an Affymetrix GCS3000 7G system
| Sample_data_processing | RMA normalization of the data followed by two-way ANOVAs using the default analysis settings in Array Studio
| Sample_platform_id | GPL1261
| Sample_contact_name | Andrew,,MCKENZIE
| Sample_contact_email | anm@mrc-lmb.cam.ac.uk
| Sample_contact_phone | 44 1223 402377
| Sample_contact_institute | MRC-LMB
| Sample_contact_address | HILLS ROAD
| Sample_contact_city | CAMBRIDGE
| Sample_contact_zip/postal_code | CB2 0QH
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM635nnn/GSM635587/suppl/GSM635587_Day_9_Nuocyte3.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM635nnn/GSM635587/suppl/GSM635587_Day_9_Nuocyte3.rma.chp.gz
| Sample_series_id | GSE25890
| Sample_data_row_count | 45101
| |
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