Home   Search   Browse   Statistics   User guide   FAQs   Post question   Download   Process data  
Search results for the GEO ID: GSE2600
(Click on the check boxes provided under "Select for analysis", to initiate grouping)
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down)
GSM ID
GPL ID
Select for analysis
Title
Source name
Description
Characteristics
GSM49939
GPL570
NB4 CONTROL 1 NB4 PROMYELOCYTIC CELLS We used the Human Genome U133 Plus 2.0 Array (Affymetrix) containing 54,675 probe sets to determine global impacts of A. phagocytophilum infection in NB4 cells at four hours post-infection. The mRNA was isolated using the TRI reagent extraction protocol (Molecular Research Center, Cincinnati) followed by RNeasy cleanup. cRNA production, labeling reactions, hybridization, staining and washing were performed according to the Gene Chip Expression Analysis Technical Manual (Affymetrix, Santa Clara) at the W.M. Keck Facility (Yale University). Image data were quantified by using genechip/suite microarray suite 5.0 (MAS 5.0) and default parameters were used for identification of informative probes. The presence of transcripts was generated according to a detection p-value described in the Statistical Algorithms Reference Guide (Affymetrix, Santa Clara). The one-sided Wilcoxon’s Signed Rank was the statistical method employed to generate detection p-values. Transcripts with a p-value lower than 0.05 were considered present in RNA samples. Global scaling was used to remove differences and facilitate comparison analysis.
GSM49940
GPL570
NB4 CONTROL 2 NB4 PROMYELOCYTIC CELLS We used the Human Genome U133 Plus 2.0 Array (Affymetrix) containing 54,675 probe sets to determine global impacts of A. phagocytophilum infection in NB4 cells at four hours post-infection. The mRNA was isolated using the TRI reagent extraction protocol (Molecular Research Center, Cincinnati) followed by RNeasy cleanup. cRNA production, labeling reactions, hybridization, staining and washing were performed according to the Gene Chip Expression Analysis Technical Manual (Affymetrix, Santa Clara) at the W.M. Keck Facility (Yale University). Image data were quantified by using genechip/suite microarray suite 5.0 (MAS 5.0) and default parameters were used for identification of informative probes.
GSM49941
GPL570
NB4 CONTROL 3 NB4 PROMYELOCYTIC CELLS We used the Human Genome U133 Plus 2.0 Array (Affymetrix) containing 54,675 probe sets to determine global impacts of A. phagocytophilum infection in NB4 cells at four hours post-infection. The mRNA was isolated using the TRI reagent extraction protocol (Molecular Research Center, Cincinnati) followed by RNeasy cleanup. cRNA production, labeling reactions, hybridization, staining and washing were performed according to the Gene Chip Expression Analysis Technical Manual (Affymetrix, Santa Clara) at the W.M. Keck Facility (Yale University). Image data were quantified by using genechip/suite microarray suite 5.0 (MAS 5.0) and default parameters were used for identification of informative probes.
GSM49942
GPL570
NB4 INFECTED 1 NB4 PROMYELOCYTIC CELLS We used the Human Genome U133 Plus 2.0 Array (Affymetrix) containing 54,675 probe sets to determine global impacts of A. phagocytophilum infection in NB4 cells at four hours post-infection. The mRNA was isolated using the TRI reagent extraction protocol (Molecular Research Center, Cincinnati) followed by RNeasy cleanup. cRNA production, labeling reactions, hybridization, staining and washing were performed according to the Gene Chip Expression Analysis Technical Manual (Affymetrix, Santa Clara) at the W.M. Keck Facility (Yale University). Image data were quantified by using genechip/suite microarray suite 5.0 (MAS 5.0) and default parameters were used for identification of informative probes.
GSM49943
GPL570
NB4 INFECTED 2 NB4 PROMYELOCYTIC CELLS We used the Human Genome U133 Plus 2.0 Array (Affymetrix) containing 54,675 probe sets to determine global impacts of A. phagocytophilum infection in NB4 cells at four hours post-infection. The mRNA was isolated using the TRI reagent extraction protocol (Molecular Research Center, Cincinnati) followed by RNeasy cleanup. cRNA production, labeling reactions, hybridization, staining and washing were performed according to the Gene Chip Expression Analysis Technical Manual (Affymetrix, Santa Clara) at the W.M. Keck Facility (Yale University). Image data were quantified by using genechip/suite microarray suite 5.0 (MAS 5.0) and default parameters were used for identification of informative probes.
GSM49944
GPL570
NB4 INFECTED 3 NB4 PROMYELOCYTIC CELLS We used the Human Genome U133 Plus 2.0 Array (Affymetrix) containing 54,675 probe sets to determine global impacts of A. phagocytophilum infection in NB4 cells at four hours post-infection. The mRNA was isolated using the TRI reagent extraction protocol (Molecular Research Center, Cincinnati) followed by RNeasy cleanup. cRNA production, labeling reactions, hybridization, staining and washing were performed according to the Gene Chip Expression Analysis Technical Manual (Affymetrix, Santa Clara) at the W.M. Keck Facility (Yale University). Image data were quantified by using genechip/suite microarray suite 5.0 (MAS 5.0) and default parameters were used for identification of informative probes.
 
 
Make groups for comparisons
(2 groups will be compared at a time)
Select GSMs and click on "Add groups"
Enter the group name here:


Select expression type
Transcripts profile based on;
A. Differential status (Up/Down regulation)
B. Absolute calls (Transcribed/Not-detected)
 
Filter results by number of probes