Search results for the GEO ID: GSE26023  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM638726 | GPL1261 |
|
phd3 null sample 1
|
phd3 null 110608
|
genotype: phd3 null
cell type: neutrophil
treatment: hypoxia
genetic background: C57
|
From each individual mouse neutrophils were isolated by negative magnetic selection
|
| Sample_geo_accession | GSM638726
| | Sample_status | Public on Feb 08 2011
| | Sample_submission_date | Dec 11 2010
| | Sample_last_update_date | Feb 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | mirVana total RNA extraction was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430_2 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | Affymetrix data was MAS 5.0 normalised and log fold change determined using PANTHER software
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Sarah,Ruth,Walmsley
| | Sample_contact_email | s.walmsley@sheffield.ac.uk
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | Department of Infection and Immunity, The Medical School, Beech Hill Road
| | Sample_contact_city | Sheffield
| | Sample_contact_zip/postal_code | S10 2RX
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM638nnn/GSM638726/suppl/GSM638726_-3-3_110608.CEL.gz
| | Sample_series_id | GSE26023
| | Sample_data_row_count | 45101
| | |
|
GSM638727 | GPL1261 |
|
phd3 null sample 2
|
phd3 null 120608
|
genotype: phd3 null
cell type: neutrophil
treatment: hypoxia
genetic background: C57
|
From each individual mouse neutrophils were isolated by negative magnetic selection
|
| Sample_geo_accession | GSM638727
| | Sample_status | Public on Feb 08 2011
| | Sample_submission_date | Dec 11 2010
| | Sample_last_update_date | Feb 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | mirVana total RNA extraction was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430_2 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | Affymetrix data was MAS 5.0 normalised and log fold change determined using PANTHER software
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Sarah,Ruth,Walmsley
| | Sample_contact_email | s.walmsley@sheffield.ac.uk
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | Department of Infection and Immunity, The Medical School, Beech Hill Road
| | Sample_contact_city | Sheffield
| | Sample_contact_zip/postal_code | S10 2RX
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM638nnn/GSM638727/suppl/GSM638727_-3-3_120608.CEL.gz
| | Sample_series_id | GSE26023
| | Sample_data_row_count | 45101
| | |
|
GSM638728 | GPL1261 |
|
phd3 null sample 3
|
phd3 null 130608
|
genotype: phd3 null
cell type: neutrophil
treatment: hypoxia
genetic background: C57
|
From each individual mouse neutrophils were isolated by negative magnetic selection
|
| Sample_geo_accession | GSM638728
| | Sample_status | Public on Feb 08 2011
| | Sample_submission_date | Dec 11 2010
| | Sample_last_update_date | Feb 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | mirVana total RNA extraction was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430_2 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | Affymetrix data was MAS 5.0 normalised and log fold change determined using PANTHER software
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Sarah,Ruth,Walmsley
| | Sample_contact_email | s.walmsley@sheffield.ac.uk
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | Department of Infection and Immunity, The Medical School, Beech Hill Road
| | Sample_contact_city | Sheffield
| | Sample_contact_zip/postal_code | S10 2RX
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM638nnn/GSM638728/suppl/GSM638728_-3-3_130608.CEL.gz
| | Sample_series_id | GSE26023
| | Sample_data_row_count | 45101
| | |
|
GSM638729 | GPL1261 |
|
wild type sample 1
|
bl6 110608
|
genotype: wild type mouse
cell type: neutrophil
treatment: hypoxia
genetic background: C57
|
From each individual mouse neutrophils were isolated by negative magnetic selection
|
| Sample_geo_accession | GSM638729
| | Sample_status | Public on Feb 08 2011
| | Sample_submission_date | Dec 11 2010
| | Sample_last_update_date | Feb 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | mirVana total RNA extraction was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430_2 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | Affymetrix data was MAS 5.0 normalised and log fold change determined using PANTHER software
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Sarah,Ruth,Walmsley
| | Sample_contact_email | s.walmsley@sheffield.ac.uk
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | Department of Infection and Immunity, The Medical School, Beech Hill Road
| | Sample_contact_city | Sheffield
| | Sample_contact_zip/postal_code | S10 2RX
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM638nnn/GSM638729/suppl/GSM638729_Bl6_110608.CEL.gz
| | Sample_series_id | GSE26023
| | Sample_data_row_count | 45101
| | |
|
GSM638730 | GPL1261 |
|
wild type sample 2
|
bl6 120608
|
genotype: wild type mouse
cell type: neutrophil
treatment: hypoxia
genetic background: C57
|
From each individual mouse neutrophils were isolated by negative magnetic selection
|
| Sample_geo_accession | GSM638730
| | Sample_status | Public on Feb 08 2011
| | Sample_submission_date | Dec 11 2010
| | Sample_last_update_date | Feb 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | mirVana total RNA extraction was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430_2 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | Affymetrix data was MAS 5.0 normalised and log fold change determined using PANTHER software
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Sarah,Ruth,Walmsley
| | Sample_contact_email | s.walmsley@sheffield.ac.uk
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | Department of Infection and Immunity, The Medical School, Beech Hill Road
| | Sample_contact_city | Sheffield
| | Sample_contact_zip/postal_code | S10 2RX
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM638nnn/GSM638730/suppl/GSM638730_Bl6_120608.CEL.gz
| | Sample_series_id | GSE26023
| | Sample_data_row_count | 45101
| | |
|
GSM638731 | GPL1261 |
|
wild type sample 3
|
bl6 130608
|
genotype: wild type mouse
cell type: neutrophil
treatment: hypoxia
genetic background: C57
|
From each individual mouse neutrophils were isolated by negative magnetic selection
|
| Sample_geo_accession | GSM638731
| | Sample_status | Public on Feb 08 2011
| | Sample_submission_date | Dec 11 2010
| | Sample_last_update_date | Feb 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | mirVana total RNA extraction was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430_2 GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | Affymetrix data was MAS 5.0 normalised and log fold change determined using PANTHER software
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Sarah,Ruth,Walmsley
| | Sample_contact_email | s.walmsley@sheffield.ac.uk
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | Department of Infection and Immunity, The Medical School, Beech Hill Road
| | Sample_contact_city | Sheffield
| | Sample_contact_zip/postal_code | S10 2RX
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM638nnn/GSM638731/suppl/GSM638731_Bl6_130608.CEL.gz
| | Sample_series_id | GSE26023
| | Sample_data_row_count | 45101
| | |
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