Search results for the GEO ID: GSE26290 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM645604 | GPL1261 |
|
Tamoxifen Cre Control CTL, biological replicate 1
|
CTL made from Tamox Cre splenocytes and treated with 4OHT
|
strain: C57BL/6
cell type: cytotoxic T-lymphocytes (CTL)
genotype/variation: control
|
Gene expression data from C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) control CTL treated with 4OHT
TamoxCreKG167
|
Sample_geo_accession | GSM645604
| Sample_status | Public on Feb 01 2011
| Sample_submission_date | Dec 23 2010
| Sample_last_update_date | Feb 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) and PDK1fl/fl C57BL/6GT(ROSA)26tm9(creEsr1)Arte (PDK1fl/fl TamoxCre) T-cells were activated for 48h and then cultured for 5days, supplimented with 0.6μM 4OHT for the last 72h of culture.
| Sample_treatment_protocol_ch1 | Alternatively T-cells from C57/B6 mice were were activated for 48h and then cultured for 5days with half the cells from each biological replicated being treated with 1μM AktI-1/2 for the last 48h of culture.
| Sample_growth_protocol_ch1 | CTL were generated by activating Splenic T-cells for 48h in RPMI 1640/10% heat-inactivated fetal calf serum (FCS) penicillin/streptomycin (Gibco), 50μM β-mercaptoethanol (β-ME) (Sigma) with 0.5μg/ml CD3 antibody (2C11) in the presence of 20ng/ml IL2.
| Sample_growth_protocol_ch1 | Cells were then washed free of activating agent and thereafter maintained in 20ng/ml IL2 at a density of approximately 0.3×106/ml at 37°C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted from cells with the RNeasy RNA purification minikit (Qiagen) according to the manufactures protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2μg RNA according to the standard One-cycle target labelling protocol from GeneChip Expression Analysis Technical Manual (with specific protocols for using the GeneChip hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | Each sample was hybridized to GeneChip®Mouse Genome 430 2.0 Array at +45°C overnight (16 h) according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | GeneChip Fluidics Station 450 was used to wash and stain the arrays.
| Sample_scan_protocol | GeneChip Scanner 3000 7G with AutoLoader was used to scan the arrays.
| Sample_data_processing | Affymetrix GeneChip Command Console (AGCC) 1.1 was used to control GeneChip Fluidics Stations and Scanner and in summarizing probe cell intensity data (.CEL file generation).
| Sample_data_processing | After hybridization quality was checked with AGCC and Expression Console TM 1.1.
| Sample_data_processing | Data was normalized using the Affymetrix Expression Console v1.1 (Affymetrix) implementation of the robust multichip averaging algorithm using default analysis settings and quantile normalisation.
| Sample_platform_id | GPL1261
| Sample_contact_name | maria,carmen,feijoo
| Sample_contact_email | c.feijoocarnero@dundee.ac.uk
| Sample_contact_laboratory | Cantrell lab
| Sample_contact_department | Cell biology and immunology
| Sample_contact_institute | University of Dundee
| Sample_contact_address | Dow Street
| Sample_contact_city | Dundee
| Sample_contact_zip/postal_code | dd15eh
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM645nnn/GSM645604/suppl/GSM645604.CEL.gz
| Sample_series_id | GSE26290
| Sample_data_row_count | 45101
| |
|
GSM645605 | GPL1261 |
|
Tamoxifen Cre Control CTL, biological replicate 2
|
CTL made from Tamox Cre splenocytes and treated with 4OHT
|
strain: C57BL/6
cell type: cytotoxic T-lymphocytes (CTL)
genotype/variation: control
|
Gene expression data from C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) control CTL treated with 4OHT
TamoxCreKG168
|
Sample_geo_accession | GSM645605
| Sample_status | Public on Feb 01 2011
| Sample_submission_date | Dec 23 2010
| Sample_last_update_date | Feb 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) and PDK1fl/fl C57BL/6GT(ROSA)26tm9(creEsr1)Arte (PDK1fl/fl TamoxCre) T-cells were activated for 48h and then cultured for 5days, supplimented with 0.6μM 4OHT for the last 72h of culture.
| Sample_treatment_protocol_ch1 | Alternatively T-cells from C57/B6 mice were were activated for 48h and then cultured for 5days with half the cells from each biological replicated being treated with 1μM AktI-1/2 for the last 48h of culture.
| Sample_growth_protocol_ch1 | CTL were generated by activating Splenic T-cells for 48h in RPMI 1640/10% heat-inactivated fetal calf serum (FCS) penicillin/streptomycin (Gibco), 50μM β-mercaptoethanol (β-ME) (Sigma) with 0.5μg/ml CD3 antibody (2C11) in the presence of 20ng/ml IL2.
| Sample_growth_protocol_ch1 | Cells were then washed free of activating agent and thereafter maintained in 20ng/ml IL2 at a density of approximately 0.3×106/ml at 37°C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted from cells with the RNeasy RNA purification minikit (Qiagen) according to the manufactures protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2μg RNA according to the standard One-cycle target labelling protocol from GeneChip Expression Analysis Technical Manual (with specific protocols for using the GeneChip hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | Each sample was hybridized to GeneChip®Mouse Genome 430 2.0 Array at +45°C overnight (16 h) according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | GeneChip Fluidics Station 450 was used to wash and stain the arrays.
| Sample_scan_protocol | GeneChip Scanner 3000 7G with AutoLoader was used to scan the arrays.
| Sample_data_processing | Affymetrix GeneChip Command Console (AGCC) 1.1 was used to control GeneChip Fluidics Stations and Scanner and in summarizing probe cell intensity data (.CEL file generation).
| Sample_data_processing | After hybridization quality was checked with AGCC and Expression Console TM 1.1.
| Sample_data_processing | Data was normalized using the Affymetrix Expression Console v1.1 (Affymetrix) implementation of the robust multichip averaging algorithm using default analysis settings and quantile normalisation.
| Sample_platform_id | GPL1261
| Sample_contact_name | maria,carmen,feijoo
| Sample_contact_email | c.feijoocarnero@dundee.ac.uk
| Sample_contact_laboratory | Cantrell lab
| Sample_contact_department | Cell biology and immunology
| Sample_contact_institute | University of Dundee
| Sample_contact_address | Dow Street
| Sample_contact_city | Dundee
| Sample_contact_zip/postal_code | dd15eh
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM645nnn/GSM645605/suppl/GSM645605.CEL.gz
| Sample_series_id | GSE26290
| Sample_data_row_count | 45101
| |
|
GSM645606 | GPL1261 |
|
Tamoxifen Cre Control CTL, biological replicate 3
|
CTL made from Tamox Cre splenocytes and treated with 4OHT
|
strain: C57BL/6
cell type: cytotoxic T-lymphocytes (CTL)
genotype/variation: control
|
Gene expression data from C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) control CTL treated with 4OHT
TamoxCreKG169
|
Sample_geo_accession | GSM645606
| Sample_status | Public on Feb 01 2011
| Sample_submission_date | Dec 23 2010
| Sample_last_update_date | Feb 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) and PDK1fl/fl C57BL/6GT(ROSA)26tm9(creEsr1)Arte (PDK1fl/fl TamoxCre) T-cells were activated for 48h and then cultured for 5days, supplimented with 0.6μM 4OHT for the last 72h of culture.
| Sample_treatment_protocol_ch1 | Alternatively T-cells from C57/B6 mice were were activated for 48h and then cultured for 5days with half the cells from each biological replicated being treated with 1μM AktI-1/2 for the last 48h of culture.
| Sample_growth_protocol_ch1 | CTL were generated by activating Splenic T-cells for 48h in RPMI 1640/10% heat-inactivated fetal calf serum (FCS) penicillin/streptomycin (Gibco), 50μM β-mercaptoethanol (β-ME) (Sigma) with 0.5μg/ml CD3 antibody (2C11) in the presence of 20ng/ml IL2.
| Sample_growth_protocol_ch1 | Cells were then washed free of activating agent and thereafter maintained in 20ng/ml IL2 at a density of approximately 0.3×106/ml at 37°C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted from cells with the RNeasy RNA purification minikit (Qiagen) according to the manufactures protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2μg RNA according to the standard One-cycle target labelling protocol from GeneChip Expression Analysis Technical Manual (with specific protocols for using the GeneChip hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | Each sample was hybridized to GeneChip®Mouse Genome 430 2.0 Array at +45°C overnight (16 h) according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | GeneChip Fluidics Station 450 was used to wash and stain the arrays.
| Sample_scan_protocol | GeneChip Scanner 3000 7G with AutoLoader was used to scan the arrays.
| Sample_data_processing | Affymetrix GeneChip Command Console (AGCC) 1.1 was used to control GeneChip Fluidics Stations and Scanner and in summarizing probe cell intensity data (.CEL file generation).
| Sample_data_processing | After hybridization quality was checked with AGCC and Expression Console TM 1.1.
| Sample_data_processing | Data was normalized using the Affymetrix Expression Console v1.1 (Affymetrix) implementation of the robust multichip averaging algorithm using default analysis settings and quantile normalisation.
| Sample_platform_id | GPL1261
| Sample_contact_name | maria,carmen,feijoo
| Sample_contact_email | c.feijoocarnero@dundee.ac.uk
| Sample_contact_laboratory | Cantrell lab
| Sample_contact_department | Cell biology and immunology
| Sample_contact_institute | University of Dundee
| Sample_contact_address | Dow Street
| Sample_contact_city | Dundee
| Sample_contact_zip/postal_code | dd15eh
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM645nnn/GSM645606/suppl/GSM645606.CEL.gz
| Sample_series_id | GSE26290
| Sample_data_row_count | 45101
| |
|
GSM645607 | GPL1261 |
|
PDK1 null CTL, biological replicate 1
|
CTL made from PDK1fl/fl TamoxCre splenocytes and treated with 4OHT
|
strain: C57BL/6
cell type: cytotoxic T-lymphocytes (CTL)
genotype/variation: PDK1 null
|
Gene expression data from PDK1 null CTL ( C57BL/6GT(ROSA)26tm9(creEsr1)Arte PDK1fl/fl treated with 4OHT)
TamoxCrePDK1KC248
|
Sample_geo_accession | GSM645607
| Sample_status | Public on Feb 01 2011
| Sample_submission_date | Dec 23 2010
| Sample_last_update_date | Feb 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) and PDK1fl/fl C57BL/6GT(ROSA)26tm9(creEsr1)Arte (PDK1fl/fl TamoxCre) T-cells were activated for 48h and then cultured for 5days, supplimented with 0.6μM 4OHT for the last 72h of culture.
| Sample_treatment_protocol_ch1 | Alternatively T-cells from C57/B6 mice were were activated for 48h and then cultured for 5days with half the cells from each biological replicated being treated with 1μM AktI-1/2 for the last 48h of culture.
| Sample_growth_protocol_ch1 | CTL were generated by activating Splenic T-cells for 48h in RPMI 1640/10% heat-inactivated fetal calf serum (FCS) penicillin/streptomycin (Gibco), 50μM β-mercaptoethanol (β-ME) (Sigma) with 0.5μg/ml CD3 antibody (2C11) in the presence of 20ng/ml IL2.
| Sample_growth_protocol_ch1 | Cells were then washed free of activating agent and thereafter maintained in 20ng/ml IL2 at a density of approximately 0.3×106/ml at 37°C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted from cells with the RNeasy RNA purification minikit (Qiagen) according to the manufactures protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2μg RNA according to the standard One-cycle target labelling protocol from GeneChip Expression Analysis Technical Manual (with specific protocols for using the GeneChip hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | Each sample was hybridized to GeneChip®Mouse Genome 430 2.0 Array at +45°C overnight (16 h) according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | GeneChip Fluidics Station 450 was used to wash and stain the arrays.
| Sample_scan_protocol | GeneChip Scanner 3000 7G with AutoLoader was used to scan the arrays.
| Sample_data_processing | Affymetrix GeneChip Command Console (AGCC) 1.1 was used to control GeneChip Fluidics Stations and Scanner and in summarizing probe cell intensity data (.CEL file generation).
| Sample_data_processing | After hybridization quality was checked with AGCC and Expression Console TM 1.1.
| Sample_data_processing | Data was normalized using the Affymetrix Expression Console v1.1 (Affymetrix) implementation of the robust multichip averaging algorithm using default analysis settings and quantile normalisation.
| Sample_platform_id | GPL1261
| Sample_contact_name | maria,carmen,feijoo
| Sample_contact_email | c.feijoocarnero@dundee.ac.uk
| Sample_contact_laboratory | Cantrell lab
| Sample_contact_department | Cell biology and immunology
| Sample_contact_institute | University of Dundee
| Sample_contact_address | Dow Street
| Sample_contact_city | Dundee
| Sample_contact_zip/postal_code | dd15eh
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM645nnn/GSM645607/suppl/GSM645607.CEL.gz
| Sample_series_id | GSE26290
| Sample_data_row_count | 45101
| |
|
GSM645608 | GPL1261 |
|
PDK1 null CTL, biological replicate 2
|
CTL made from PDK1fl/fl TamoxCre splenocytes and treated with 4OHT
|
strain: C57BL/6
cell type: cytotoxic T-lymphocytes (CTL)
genotype/variation: PDK1 null
|
Gene expression data from PDK1 null CTL ( C57BL/6GT(ROSA)26tm9(creEsr1)Arte PDK1fl/fl treated with 4OHT)
TamoxCrePDK1KC252
|
Sample_geo_accession | GSM645608
| Sample_status | Public on Feb 01 2011
| Sample_submission_date | Dec 23 2010
| Sample_last_update_date | Feb 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) and PDK1fl/fl C57BL/6GT(ROSA)26tm9(creEsr1)Arte (PDK1fl/fl TamoxCre) T-cells were activated for 48h and then cultured for 5days, supplimented with 0.6μM 4OHT for the last 72h of culture.
| Sample_treatment_protocol_ch1 | Alternatively T-cells from C57/B6 mice were were activated for 48h and then cultured for 5days with half the cells from each biological replicated being treated with 1μM AktI-1/2 for the last 48h of culture.
| Sample_growth_protocol_ch1 | CTL were generated by activating Splenic T-cells for 48h in RPMI 1640/10% heat-inactivated fetal calf serum (FCS) penicillin/streptomycin (Gibco), 50μM β-mercaptoethanol (β-ME) (Sigma) with 0.5μg/ml CD3 antibody (2C11) in the presence of 20ng/ml IL2.
| Sample_growth_protocol_ch1 | Cells were then washed free of activating agent and thereafter maintained in 20ng/ml IL2 at a density of approximately 0.3×106/ml at 37°C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted from cells with the RNeasy RNA purification minikit (Qiagen) according to the manufactures protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2μg RNA according to the standard One-cycle target labelling protocol from GeneChip Expression Analysis Technical Manual (with specific protocols for using the GeneChip hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | Each sample was hybridized to GeneChip®Mouse Genome 430 2.0 Array at +45°C overnight (16 h) according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | GeneChip Fluidics Station 450 was used to wash and stain the arrays.
| Sample_scan_protocol | GeneChip Scanner 3000 7G with AutoLoader was used to scan the arrays.
| Sample_data_processing | Affymetrix GeneChip Command Console (AGCC) 1.1 was used to control GeneChip Fluidics Stations and Scanner and in summarizing probe cell intensity data (.CEL file generation).
| Sample_data_processing | After hybridization quality was checked with AGCC and Expression Console TM 1.1.
| Sample_data_processing | Data was normalized using the Affymetrix Expression Console v1.1 (Affymetrix) implementation of the robust multichip averaging algorithm using default analysis settings and quantile normalisation.
| Sample_platform_id | GPL1261
| Sample_contact_name | maria,carmen,feijoo
| Sample_contact_email | c.feijoocarnero@dundee.ac.uk
| Sample_contact_laboratory | Cantrell lab
| Sample_contact_department | Cell biology and immunology
| Sample_contact_institute | University of Dundee
| Sample_contact_address | Dow Street
| Sample_contact_city | Dundee
| Sample_contact_zip/postal_code | dd15eh
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM645nnn/GSM645608/suppl/GSM645608.CEL.gz
| Sample_series_id | GSE26290
| Sample_data_row_count | 45101
| |
|
GSM645609 | GPL1261 |
|
PDK1 null CTL, biological replicate 3
|
CTL made from PDK1fl/fl TamoxCre splenocytes and treated with 4OHT
|
strain: C57BL/6
cell type: cytotoxic T-lymphocytes (CTL)
genotype/variation: PDK1 null
|
Gene expression data from PDK1 null CTL ( C57BL/6GT(ROSA)26tm9(creEsr1)Arte PDK1fl/fl treated with 4OHT)
TamoxCrePDK1KC283
|
Sample_geo_accession | GSM645609
| Sample_status | Public on Feb 01 2011
| Sample_submission_date | Dec 23 2010
| Sample_last_update_date | Feb 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) and PDK1fl/fl C57BL/6GT(ROSA)26tm9(creEsr1)Arte (PDK1fl/fl TamoxCre) T-cells were activated for 48h and then cultured for 5days, supplimented with 0.6μM 4OHT for the last 72h of culture.
| Sample_treatment_protocol_ch1 | Alternatively T-cells from C57/B6 mice were were activated for 48h and then cultured for 5days with half the cells from each biological replicated being treated with 1μM AktI-1/2 for the last 48h of culture.
| Sample_growth_protocol_ch1 | CTL were generated by activating Splenic T-cells for 48h in RPMI 1640/10% heat-inactivated fetal calf serum (FCS) penicillin/streptomycin (Gibco), 50μM β-mercaptoethanol (β-ME) (Sigma) with 0.5μg/ml CD3 antibody (2C11) in the presence of 20ng/ml IL2.
| Sample_growth_protocol_ch1 | Cells were then washed free of activating agent and thereafter maintained in 20ng/ml IL2 at a density of approximately 0.3×106/ml at 37°C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted from cells with the RNeasy RNA purification minikit (Qiagen) according to the manufactures protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2μg RNA according to the standard One-cycle target labelling protocol from GeneChip Expression Analysis Technical Manual (with specific protocols for using the GeneChip hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | Each sample was hybridized to GeneChip®Mouse Genome 430 2.0 Array at +45°C overnight (16 h) according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | GeneChip Fluidics Station 450 was used to wash and stain the arrays.
| Sample_scan_protocol | GeneChip Scanner 3000 7G with AutoLoader was used to scan the arrays.
| Sample_data_processing | Affymetrix GeneChip Command Console (AGCC) 1.1 was used to control GeneChip Fluidics Stations and Scanner and in summarizing probe cell intensity data (.CEL file generation).
| Sample_data_processing | After hybridization quality was checked with AGCC and Expression Console TM 1.1.
| Sample_data_processing | Data was normalized using the Affymetrix Expression Console v1.1 (Affymetrix) implementation of the robust multichip averaging algorithm using default analysis settings and quantile normalisation.
| Sample_platform_id | GPL1261
| Sample_contact_name | maria,carmen,feijoo
| Sample_contact_email | c.feijoocarnero@dundee.ac.uk
| Sample_contact_laboratory | Cantrell lab
| Sample_contact_department | Cell biology and immunology
| Sample_contact_institute | University of Dundee
| Sample_contact_address | Dow Street
| Sample_contact_city | Dundee
| Sample_contact_zip/postal_code | dd15eh
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM645nnn/GSM645609/suppl/GSM645609.CEL.gz
| Sample_series_id | GSE26290
| Sample_data_row_count | 45101
| |
|
GSM645610 | GPL1261 |
|
wild type CTL, biological replicate 1
|
CTL made from wild type splenocytes
|
strain: C57BL/6
cell type: cytotoxic T-lymphocytes (CTL)
genotype/variation: wild type
agent: control
|
Gene expression data from wild-type CTL from mouse 1
1_152IL2
|
Sample_geo_accession | GSM645610
| Sample_status | Public on Feb 01 2011
| Sample_submission_date | Dec 23 2010
| Sample_last_update_date | Feb 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) and PDK1fl/fl C57BL/6GT(ROSA)26tm9(creEsr1)Arte (PDK1fl/fl TamoxCre) T-cells were activated for 48h and then cultured for 5days, supplimented with 0.6μM 4OHT for the last 72h of culture.
| Sample_treatment_protocol_ch1 | Alternatively T-cells from C57/B6 mice were were activated for 48h and then cultured for 5days with half the cells from each biological replicated being treated with 1μM AktI-1/2 for the last 48h of culture.
| Sample_growth_protocol_ch1 | CTL were generated by activating Splenic T-cells for 48h in RPMI 1640/10% heat-inactivated fetal calf serum (FCS) penicillin/streptomycin (Gibco), 50μM β-mercaptoethanol (β-ME) (Sigma) with 0.5μg/ml CD3 antibody (2C11) in the presence of 20ng/ml IL2.
| Sample_growth_protocol_ch1 | Cells were then washed free of activating agent and thereafter maintained in 20ng/ml IL2 at a density of approximately 0.3×106/ml at 37°C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted from cells with the RNeasy RNA purification minikit (Qiagen) according to the manufactures protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2μg RNA according to the standard One-cycle target labelling protocol from GeneChip Expression Analysis Technical Manual (with specific protocols for using the GeneChip hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | Each sample was hybridized to GeneChip®Mouse Genome 430 2.0 Array at +45°C overnight (16 h) according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | GeneChip Fluidics Station 450 was used to wash and stain the arrays.
| Sample_scan_protocol | GeneChip Scanner 3000 7G with AutoLoader was used to scan the arrays.
| Sample_data_processing | Affymetrix GeneChip Command Console (AGCC) 1.1 was used to control GeneChip Fluidics Stations and Scanner and in summarizing probe cell intensity data (.CEL file generation).
| Sample_data_processing | After hybridization quality was checked with AGCC and Expression Console TM 1.1.
| Sample_data_processing | Data was normalized using the Affymetrix Expression Console v1.1 (Affymetrix) implementation of the robust multichip averaging algorithm using default analysis settings and quantile normalisation.
| Sample_platform_id | GPL1261
| Sample_contact_name | maria,carmen,feijoo
| Sample_contact_email | c.feijoocarnero@dundee.ac.uk
| Sample_contact_laboratory | Cantrell lab
| Sample_contact_department | Cell biology and immunology
| Sample_contact_institute | University of Dundee
| Sample_contact_address | Dow Street
| Sample_contact_city | Dundee
| Sample_contact_zip/postal_code | dd15eh
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM645nnn/GSM645610/suppl/GSM645610.CEL.gz
| Sample_series_id | GSE26290
| Sample_data_row_count | 45101
| |
|
GSM645611 | GPL1261 |
|
wild type CTL+AktI, biological replicate 1
|
CTL made from wild type splenocytes and treated with AktI
|
strain: C57BL/6
cell type: cytotoxic T-lymphocytes (CTL)
genotype/variation: wild type
agent: Akt inhibitor AktI-1/2
|
Gene expression data from wild-type CTL from mouse 1 treated with AktI
2_153IL2Akti
|
Sample_geo_accession | GSM645611
| Sample_status | Public on Feb 01 2011
| Sample_submission_date | Dec 23 2010
| Sample_last_update_date | Feb 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) and PDK1fl/fl C57BL/6GT(ROSA)26tm9(creEsr1)Arte (PDK1fl/fl TamoxCre) T-cells were activated for 48h and then cultured for 5days, supplimented with 0.6μM 4OHT for the last 72h of culture.
| Sample_treatment_protocol_ch1 | Alternatively T-cells from C57/B6 mice were were activated for 48h and then cultured for 5days with half the cells from each biological replicated being treated with 1μM AktI-1/2 for the last 48h of culture.
| Sample_growth_protocol_ch1 | CTL were generated by activating Splenic T-cells for 48h in RPMI 1640/10% heat-inactivated fetal calf serum (FCS) penicillin/streptomycin (Gibco), 50μM β-mercaptoethanol (β-ME) (Sigma) with 0.5μg/ml CD3 antibody (2C11) in the presence of 20ng/ml IL2.
| Sample_growth_protocol_ch1 | Cells were then washed free of activating agent and thereafter maintained in 20ng/ml IL2 at a density of approximately 0.3×106/ml at 37°C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted from cells with the RNeasy RNA purification minikit (Qiagen) according to the manufactures protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2μg RNA according to the standard One-cycle target labelling protocol from GeneChip Expression Analysis Technical Manual (with specific protocols for using the GeneChip hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | Each sample was hybridized to GeneChip®Mouse Genome 430 2.0 Array at +45°C overnight (16 h) according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | GeneChip Fluidics Station 450 was used to wash and stain the arrays.
| Sample_scan_protocol | GeneChip Scanner 3000 7G with AutoLoader was used to scan the arrays.
| Sample_data_processing | Affymetrix GeneChip Command Console (AGCC) 1.1 was used to control GeneChip Fluidics Stations and Scanner and in summarizing probe cell intensity data (.CEL file generation).
| Sample_data_processing | After hybridization quality was checked with AGCC and Expression Console TM 1.1.
| Sample_data_processing | Data was normalized using the Affymetrix Expression Console v1.1 (Affymetrix) implementation of the robust multichip averaging algorithm using default analysis settings and quantile normalisation.
| Sample_platform_id | GPL1261
| Sample_contact_name | maria,carmen,feijoo
| Sample_contact_email | c.feijoocarnero@dundee.ac.uk
| Sample_contact_laboratory | Cantrell lab
| Sample_contact_department | Cell biology and immunology
| Sample_contact_institute | University of Dundee
| Sample_contact_address | Dow Street
| Sample_contact_city | Dundee
| Sample_contact_zip/postal_code | dd15eh
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM645nnn/GSM645611/suppl/GSM645611.CEL.gz
| Sample_series_id | GSE26290
| Sample_data_row_count | 45101
| |
|
GSM645612 | GPL1261 |
|
wild type CTL, biological replicate 2
|
CTL made from wild type splenocytes
|
strain: C57BL/6
cell type: cytotoxic T-lymphocytes (CTL)
genotype/variation: wild type
agent: control
|
Gene expression data from wild-type CTL from mouse 2
3_154IL2
|
Sample_geo_accession | GSM645612
| Sample_status | Public on Feb 01 2011
| Sample_submission_date | Dec 23 2010
| Sample_last_update_date | Feb 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) and PDK1fl/fl C57BL/6GT(ROSA)26tm9(creEsr1)Arte (PDK1fl/fl TamoxCre) T-cells were activated for 48h and then cultured for 5days, supplimented with 0.6μM 4OHT for the last 72h of culture.
| Sample_treatment_protocol_ch1 | Alternatively T-cells from C57/B6 mice were were activated for 48h and then cultured for 5days with half the cells from each biological replicated being treated with 1μM AktI-1/2 for the last 48h of culture.
| Sample_growth_protocol_ch1 | CTL were generated by activating Splenic T-cells for 48h in RPMI 1640/10% heat-inactivated fetal calf serum (FCS) penicillin/streptomycin (Gibco), 50μM β-mercaptoethanol (β-ME) (Sigma) with 0.5μg/ml CD3 antibody (2C11) in the presence of 20ng/ml IL2.
| Sample_growth_protocol_ch1 | Cells were then washed free of activating agent and thereafter maintained in 20ng/ml IL2 at a density of approximately 0.3×106/ml at 37°C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted from cells with the RNeasy RNA purification minikit (Qiagen) according to the manufactures protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2μg RNA according to the standard One-cycle target labelling protocol from GeneChip Expression Analysis Technical Manual (with specific protocols for using the GeneChip hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | Each sample was hybridized to GeneChip®Mouse Genome 430 2.0 Array at +45°C overnight (16 h) according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | GeneChip Fluidics Station 450 was used to wash and stain the arrays.
| Sample_scan_protocol | GeneChip Scanner 3000 7G with AutoLoader was used to scan the arrays.
| Sample_data_processing | Affymetrix GeneChip Command Console (AGCC) 1.1 was used to control GeneChip Fluidics Stations and Scanner and in summarizing probe cell intensity data (.CEL file generation).
| Sample_data_processing | After hybridization quality was checked with AGCC and Expression Console TM 1.1.
| Sample_data_processing | Data was normalized using the Affymetrix Expression Console v1.1 (Affymetrix) implementation of the robust multichip averaging algorithm using default analysis settings and quantile normalisation.
| Sample_platform_id | GPL1261
| Sample_contact_name | maria,carmen,feijoo
| Sample_contact_email | c.feijoocarnero@dundee.ac.uk
| Sample_contact_laboratory | Cantrell lab
| Sample_contact_department | Cell biology and immunology
| Sample_contact_institute | University of Dundee
| Sample_contact_address | Dow Street
| Sample_contact_city | Dundee
| Sample_contact_zip/postal_code | dd15eh
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM645nnn/GSM645612/suppl/GSM645612.CEL.gz
| Sample_series_id | GSE26290
| Sample_data_row_count | 45101
| |
|
GSM645613 | GPL1261 |
|
wild type CTL+AktI, biological replicate 2
|
CTL made from wild type splenocytes and treated with AktI
|
strain: C57BL/6
cell type: cytotoxic T-lymphocytes (CTL)
genotype/variation: wild type
agent: Akt inhibitor AktI-1/2
|
Gene expression data from wild-type CTL from mouse 2 treated with AktI
4_155IL2Akti
|
Sample_geo_accession | GSM645613
| Sample_status | Public on Feb 01 2011
| Sample_submission_date | Dec 23 2010
| Sample_last_update_date | Feb 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) and PDK1fl/fl C57BL/6GT(ROSA)26tm9(creEsr1)Arte (PDK1fl/fl TamoxCre) T-cells were activated for 48h and then cultured for 5days, supplimented with 0.6μM 4OHT for the last 72h of culture.
| Sample_treatment_protocol_ch1 | Alternatively T-cells from C57/B6 mice were were activated for 48h and then cultured for 5days with half the cells from each biological replicated being treated with 1μM AktI-1/2 for the last 48h of culture.
| Sample_growth_protocol_ch1 | CTL were generated by activating Splenic T-cells for 48h in RPMI 1640/10% heat-inactivated fetal calf serum (FCS) penicillin/streptomycin (Gibco), 50μM β-mercaptoethanol (β-ME) (Sigma) with 0.5μg/ml CD3 antibody (2C11) in the presence of 20ng/ml IL2.
| Sample_growth_protocol_ch1 | Cells were then washed free of activating agent and thereafter maintained in 20ng/ml IL2 at a density of approximately 0.3×106/ml at 37°C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted from cells with the RNeasy RNA purification minikit (Qiagen) according to the manufactures protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2μg RNA according to the standard One-cycle target labelling protocol from GeneChip Expression Analysis Technical Manual (with specific protocols for using the GeneChip hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | Each sample was hybridized to GeneChip®Mouse Genome 430 2.0 Array at +45°C overnight (16 h) according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | GeneChip Fluidics Station 450 was used to wash and stain the arrays.
| Sample_scan_protocol | GeneChip Scanner 3000 7G with AutoLoader was used to scan the arrays.
| Sample_data_processing | Affymetrix GeneChip Command Console (AGCC) 1.1 was used to control GeneChip Fluidics Stations and Scanner and in summarizing probe cell intensity data (.CEL file generation).
| Sample_data_processing | After hybridization quality was checked with AGCC and Expression Console TM 1.1.
| Sample_data_processing | Data was normalized using the Affymetrix Expression Console v1.1 (Affymetrix) implementation of the robust multichip averaging algorithm using default analysis settings and quantile normalisation.
| Sample_platform_id | GPL1261
| Sample_contact_name | maria,carmen,feijoo
| Sample_contact_email | c.feijoocarnero@dundee.ac.uk
| Sample_contact_laboratory | Cantrell lab
| Sample_contact_department | Cell biology and immunology
| Sample_contact_institute | University of Dundee
| Sample_contact_address | Dow Street
| Sample_contact_city | Dundee
| Sample_contact_zip/postal_code | dd15eh
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM645nnn/GSM645613/suppl/GSM645613.CEL.gz
| Sample_series_id | GSE26290
| Sample_data_row_count | 45101
| |
|
GSM645614 | GPL1261 |
|
wild type CTL, biological replicate 3
|
CTL made from wild type splenocytes
|
strain: C57BL/6
cell type: cytotoxic T-lymphocytes (CTL)
genotype/variation: wild type
agent: control
|
Gene expression data from wild-type CTL from mouse 3
5_156IL2
|
Sample_geo_accession | GSM645614
| Sample_status | Public on Feb 01 2011
| Sample_submission_date | Dec 23 2010
| Sample_last_update_date | Feb 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) and PDK1fl/fl C57BL/6GT(ROSA)26tm9(creEsr1)Arte (PDK1fl/fl TamoxCre) T-cells were activated for 48h and then cultured for 5days, supplimented with 0.6μM 4OHT for the last 72h of culture.
| Sample_treatment_protocol_ch1 | Alternatively T-cells from C57/B6 mice were were activated for 48h and then cultured for 5days with half the cells from each biological replicated being treated with 1μM AktI-1/2 for the last 48h of culture.
| Sample_growth_protocol_ch1 | CTL were generated by activating Splenic T-cells for 48h in RPMI 1640/10% heat-inactivated fetal calf serum (FCS) penicillin/streptomycin (Gibco), 50μM β-mercaptoethanol (β-ME) (Sigma) with 0.5μg/ml CD3 antibody (2C11) in the presence of 20ng/ml IL2.
| Sample_growth_protocol_ch1 | Cells were then washed free of activating agent and thereafter maintained in 20ng/ml IL2 at a density of approximately 0.3×106/ml at 37°C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted from cells with the RNeasy RNA purification minikit (Qiagen) according to the manufactures protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2μg RNA according to the standard One-cycle target labelling protocol from GeneChip Expression Analysis Technical Manual (with specific protocols for using the GeneChip hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | Each sample was hybridized to GeneChip®Mouse Genome 430 2.0 Array at +45°C overnight (16 h) according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | GeneChip Fluidics Station 450 was used to wash and stain the arrays.
| Sample_scan_protocol | GeneChip Scanner 3000 7G with AutoLoader was used to scan the arrays.
| Sample_data_processing | Affymetrix GeneChip Command Console (AGCC) 1.1 was used to control GeneChip Fluidics Stations and Scanner and in summarizing probe cell intensity data (.CEL file generation).
| Sample_data_processing | After hybridization quality was checked with AGCC and Expression Console TM 1.1.
| Sample_data_processing | Data was normalized using the Affymetrix Expression Console v1.1 (Affymetrix) implementation of the robust multichip averaging algorithm using default analysis settings and quantile normalisation.
| Sample_platform_id | GPL1261
| Sample_contact_name | maria,carmen,feijoo
| Sample_contact_email | c.feijoocarnero@dundee.ac.uk
| Sample_contact_laboratory | Cantrell lab
| Sample_contact_department | Cell biology and immunology
| Sample_contact_institute | University of Dundee
| Sample_contact_address | Dow Street
| Sample_contact_city | Dundee
| Sample_contact_zip/postal_code | dd15eh
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM645nnn/GSM645614/suppl/GSM645614.CEL.gz
| Sample_series_id | GSE26290
| Sample_data_row_count | 45101
| |
|
GSM645615 | GPL1261 |
|
wild type CTL+AktI, biological replicate 3
|
CTL made from wild type splenocytes and treated with AktI
|
strain: C57BL/6
cell type: cytotoxic T-lymphocytes (CTL)
genotype/variation: wild type
agent: Akt inhibitor AktI-1/2
|
Gene expression data from wild-type CTL from mouse 3 treated with AktI
6_157IL2Akti
|
Sample_geo_accession | GSM645615
| Sample_status | Public on Feb 01 2011
| Sample_submission_date | Dec 23 2010
| Sample_last_update_date | Feb 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | C57BL/6GT(ROSA)26tm9(creEsr1)Arte (TamoxCre) and PDK1fl/fl C57BL/6GT(ROSA)26tm9(creEsr1)Arte (PDK1fl/fl TamoxCre) T-cells were activated for 48h and then cultured for 5days, supplimented with 0.6μM 4OHT for the last 72h of culture.
| Sample_treatment_protocol_ch1 | Alternatively T-cells from C57/B6 mice were were activated for 48h and then cultured for 5days with half the cells from each biological replicated being treated with 1μM AktI-1/2 for the last 48h of culture.
| Sample_growth_protocol_ch1 | CTL were generated by activating Splenic T-cells for 48h in RPMI 1640/10% heat-inactivated fetal calf serum (FCS) penicillin/streptomycin (Gibco), 50μM β-mercaptoethanol (β-ME) (Sigma) with 0.5μg/ml CD3 antibody (2C11) in the presence of 20ng/ml IL2.
| Sample_growth_protocol_ch1 | Cells were then washed free of activating agent and thereafter maintained in 20ng/ml IL2 at a density of approximately 0.3×106/ml at 37°C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was extracted from cells with the RNeasy RNA purification minikit (Qiagen) according to the manufactures protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2μg RNA according to the standard One-cycle target labelling protocol from GeneChip Expression Analysis Technical Manual (with specific protocols for using the GeneChip hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | Each sample was hybridized to GeneChip®Mouse Genome 430 2.0 Array at +45°C overnight (16 h) according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit)
| Sample_hyb_protocol | GeneChip Fluidics Station 450 was used to wash and stain the arrays.
| Sample_scan_protocol | GeneChip Scanner 3000 7G with AutoLoader was used to scan the arrays.
| Sample_data_processing | Affymetrix GeneChip Command Console (AGCC) 1.1 was used to control GeneChip Fluidics Stations and Scanner and in summarizing probe cell intensity data (.CEL file generation).
| Sample_data_processing | After hybridization quality was checked with AGCC and Expression Console TM 1.1.
| Sample_data_processing | Data was normalized using the Affymetrix Expression Console v1.1 (Affymetrix) implementation of the robust multichip averaging algorithm using default analysis settings and quantile normalisation.
| Sample_platform_id | GPL1261
| Sample_contact_name | maria,carmen,feijoo
| Sample_contact_email | c.feijoocarnero@dundee.ac.uk
| Sample_contact_laboratory | Cantrell lab
| Sample_contact_department | Cell biology and immunology
| Sample_contact_institute | University of Dundee
| Sample_contact_address | Dow Street
| Sample_contact_city | Dundee
| Sample_contact_zip/postal_code | dd15eh
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM645nnn/GSM645615/suppl/GSM645615.CEL.gz
| Sample_series_id | GSE26290
| Sample_data_row_count | 45101
| |
|
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