Search results for the GEO ID: GSE26351  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM647150 | GPL570 |
|
moPB_CD34_vehicle control biological rep 1
|
CD34 cells in erythroid differentiation medium for 2 hrs with vehicle control
|
cell type: moPB CD34+ selected cells
disease status: healthy normal volunteer
|
Gene expression data from CD34 cells in erythroid differentiation medium for 2 hrs with vehicle control
|
| Sample_geo_accession | GSM647150
| | Sample_status | Public on Oct 07 2011
| | Sample_submission_date | Dec 29 2010
| | Sample_last_update_date | Oct 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To activate the Wnt or BMP pathways, the cells were exposed to 0.5uM BIO or 25ng/ul rhBMP4, respectively, during the two hours in differentiation conditions.
| | Sample_growth_protocol_ch1 | CD34+ cells were expanded in Stemspan SFEM medium supplemented with 1XCC100 cytokine mix + 2% pen/strep. On day 6 of expansion, cells were transferred to erythroid differentiation medium (StemSpan SFEM Medium with 2% P/S, 20 ng/ml SCF, 1 U/ml Epo, 5 ng/ml IL-3, 2 uM dexamethasone, and 1 uM β-estradiol) for 2 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen was used for total RNA extraction and DNA removal.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA amplification and labeling was performed using GeneChip HT 3' IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, approximately 10 ug of cRNA were hybridized for 18 hr on Affymetrix 3' Expression GeneChip U133plus2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | Raw expression data was preprocessed by MAS5 and then log10 transformed. Biological replicates across arrays were then quantile normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Teresa,Venezia,Bowman
| | Sample_contact_laboratory | Zon Laboratory
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | One Blackfan Circle, 7th floor
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647150/suppl/GSM647150.CEL.gz
| | Sample_series_id | GSE26351
| | Sample_data_row_count | 54675
| | |
|
GSM647151 | GPL570 |
|
moPB_CD34_vehicle control biological rep 2
|
CD34 cells in erythroid differentiation medium for 2 hrs with vehicle control
|
cell type: moPB CD34+ selected cells
disease status: healthy normal volunteer
|
Gene expression data from CD34 cells in erythroid differentiation medium for 2 hrs with vehicle control
|
| Sample_geo_accession | GSM647151
| | Sample_status | Public on Oct 07 2011
| | Sample_submission_date | Dec 29 2010
| | Sample_last_update_date | Oct 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To activate the Wnt or BMP pathways, the cells were exposed to 0.5uM BIO or 25ng/ul rhBMP4, respectively, during the two hours in differentiation conditions.
| | Sample_growth_protocol_ch1 | CD34+ cells were expanded in Stemspan SFEM medium supplemented with 1XCC100 cytokine mix + 2% pen/strep. On day 6 of expansion, cells were transferred to erythroid differentiation medium (StemSpan SFEM Medium with 2% P/S, 20 ng/ml SCF, 1 U/ml Epo, 5 ng/ml IL-3, 2 uM dexamethasone, and 1 uM β-estradiol) for 2 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen was used for total RNA extraction and DNA removal.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA amplification and labeling was performed using GeneChip HT 3' IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, approximately 10 ug of cRNA were hybridized for 18 hr on Affymetrix 3' Expression GeneChip U133plus2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | Raw expression data was preprocessed by MAS5 and then log10 transformed. Biological replicates across arrays were then quantile normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Teresa,Venezia,Bowman
| | Sample_contact_laboratory | Zon Laboratory
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | One Blackfan Circle, 7th floor
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647151/suppl/GSM647151.CEL.gz
| | Sample_series_id | GSE26351
| | Sample_data_row_count | 54675
| | |
|
GSM647152 | GPL570 |
|
moPB_CD34_vehicle control biological rep 3
|
CD34 cells in erythroid differentiation medium for 2 hrs with vehicle control
|
cell type: moPB CD34+ selected cells
disease status: healthy normal volunteer
|
Gene expression data from CD34 cells in erythroid differentiation medium for 2 hrs with vehicle control
|
| Sample_geo_accession | GSM647152
| | Sample_status | Public on Oct 07 2011
| | Sample_submission_date | Dec 29 2010
| | Sample_last_update_date | Oct 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To activate the Wnt or BMP pathways, the cells were exposed to 0.5uM BIO or 25ng/ul rhBMP4, respectively, during the two hours in differentiation conditions.
| | Sample_growth_protocol_ch1 | CD34+ cells were expanded in Stemspan SFEM medium supplemented with 1XCC100 cytokine mix + 2% pen/strep. On day 6 of expansion, cells were transferred to erythroid differentiation medium (StemSpan SFEM Medium with 2% P/S, 20 ng/ml SCF, 1 U/ml Epo, 5 ng/ml IL-3, 2 uM dexamethasone, and 1 uM β-estradiol) for 2 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen was used for total RNA extraction and DNA removal.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA amplification and labeling was performed using GeneChip HT 3' IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, approximately 10 ug of cRNA were hybridized for 18 hr on Affymetrix 3' Expression GeneChip U133plus2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | Raw expression data was preprocessed by MAS5 and then log10 transformed. Biological replicates across arrays were then quantile normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Teresa,Venezia,Bowman
| | Sample_contact_laboratory | Zon Laboratory
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | One Blackfan Circle, 7th floor
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647152/suppl/GSM647152.CEL.gz
| | Sample_series_id | GSE26351
| | Sample_data_row_count | 54675
| | |
|
GSM647153 | GPL570 |
|
moPB_CD34_BIO treated biological rep 1
|
CD34 cells in erythroid differentiation medium for 2 hrs with 0.5uM BIO
|
cell type: moPB CD34+ selected cells
disease status: healthy normal volunteer
|
Gene expression data from CD34 cells in erythroid differentiation medium for 2 hrs with 0.5uM BIO
|
| Sample_geo_accession | GSM647153
| | Sample_status | Public on Oct 07 2011
| | Sample_submission_date | Dec 29 2010
| | Sample_last_update_date | Oct 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To activate the Wnt or BMP pathways, the cells were exposed to 0.5uM BIO or 25ng/ul rhBMP4, respectively, during the two hours in differentiation conditions.
| | Sample_growth_protocol_ch1 | CD34+ cells were expanded in Stemspan SFEM medium supplemented with 1XCC100 cytokine mix + 2% pen/strep. On day 6 of expansion, cells were transferred to erythroid differentiation medium (StemSpan SFEM Medium with 2% P/S, 20 ng/ml SCF, 1 U/ml Epo, 5 ng/ml IL-3, 2 uM dexamethasone, and 1 uM β-estradiol) for 2 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen was used for total RNA extraction and DNA removal.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA amplification and labeling was performed using GeneChip HT 3' IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, approximately 10 ug of cRNA were hybridized for 18 hr on Affymetrix 3' Expression GeneChip U133plus2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | Raw expression data was preprocessed by MAS5 and then log10 transformed. Biological replicates across arrays were then quantile normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Teresa,Venezia,Bowman
| | Sample_contact_laboratory | Zon Laboratory
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | One Blackfan Circle, 7th floor
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647153/suppl/GSM647153.CEL.gz
| | Sample_series_id | GSE26351
| | Sample_data_row_count | 54675
| | |
|
GSM647154 | GPL570 |
|
moPB_CD34_BIO treated biological rep 2
|
CD34 cells in erythroid differentiation medium for 2 hrs with 0.5uM BIO
|
cell type: moPB CD34+ selected cells
disease status: healthy normal volunteer
|
Gene expression data from CD34 cells in erythroid differentiation medium for 2 hrs with 0.5uM BIO
|
| Sample_geo_accession | GSM647154
| | Sample_status | Public on Oct 07 2011
| | Sample_submission_date | Dec 29 2010
| | Sample_last_update_date | Oct 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To activate the Wnt or BMP pathways, the cells were exposed to 0.5uM BIO or 25ng/ul rhBMP4, respectively, during the two hours in differentiation conditions.
| | Sample_growth_protocol_ch1 | CD34+ cells were expanded in Stemspan SFEM medium supplemented with 1XCC100 cytokine mix + 2% pen/strep. On day 6 of expansion, cells were transferred to erythroid differentiation medium (StemSpan SFEM Medium with 2% P/S, 20 ng/ml SCF, 1 U/ml Epo, 5 ng/ml IL-3, 2 uM dexamethasone, and 1 uM β-estradiol) for 2 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen was used for total RNA extraction and DNA removal.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA amplification and labeling was performed using GeneChip HT 3' IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, approximately 10 ug of cRNA were hybridized for 18 hr on Affymetrix 3' Expression GeneChip U133plus2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | Raw expression data was preprocessed by MAS5 and then log10 transformed. Biological replicates across arrays were then quantile normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Teresa,Venezia,Bowman
| | Sample_contact_laboratory | Zon Laboratory
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | One Blackfan Circle, 7th floor
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647154/suppl/GSM647154.CEL.gz
| | Sample_series_id | GSE26351
| | Sample_data_row_count | 54675
| | |
|
GSM647155 | GPL570 |
|
moPB_CD34_BIO treated biological rep 3
|
CD34 cells in erythroid differentiation medium for 2 hrs with 0.5uM BIO
|
cell type: moPB CD34+ selected cells
disease status: healthy normal volunteer
|
Gene expression data from CD34 cells in erythroid differentiation medium for 2 hrs with 0.5uM BIO
|
| Sample_geo_accession | GSM647155
| | Sample_status | Public on Oct 07 2011
| | Sample_submission_date | Dec 29 2010
| | Sample_last_update_date | Oct 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To activate the Wnt or BMP pathways, the cells were exposed to 0.5uM BIO or 25ng/ul rhBMP4, respectively, during the two hours in differentiation conditions.
| | Sample_growth_protocol_ch1 | CD34+ cells were expanded in Stemspan SFEM medium supplemented with 1XCC100 cytokine mix + 2% pen/strep. On day 6 of expansion, cells were transferred to erythroid differentiation medium (StemSpan SFEM Medium with 2% P/S, 20 ng/ml SCF, 1 U/ml Epo, 5 ng/ml IL-3, 2 uM dexamethasone, and 1 uM β-estradiol) for 2 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen was used for total RNA extraction and DNA removal.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA amplification and labeling was performed using GeneChip HT 3' IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, approximately 10 ug of cRNA were hybridized for 18 hr on Affymetrix 3' Expression GeneChip U133plus2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | Raw expression data was preprocessed by MAS5 and then log10 transformed. Biological replicates across arrays were then quantile normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Teresa,Venezia,Bowman
| | Sample_contact_laboratory | Zon Laboratory
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | One Blackfan Circle, 7th floor
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647155/suppl/GSM647155.CEL.gz
| | Sample_series_id | GSE26351
| | Sample_data_row_count | 54675
| | |
|
GSM647156 | GPL570 |
|
moPB_CD34_BMP4 treated biological rep 1
|
CD34 cells in erythroid differentiation medium for 2 hrs with 25ng/ul rhBMP4
|
cell type: moPB CD34+ selected cells
disease status: healthy normal volunteer
|
Gene expression data from CD34 cells in erythroid differentiation medium for 2 hrs with 25ng/ul rhBMP4
|
| Sample_geo_accession | GSM647156
| | Sample_status | Public on Oct 07 2011
| | Sample_submission_date | Dec 29 2010
| | Sample_last_update_date | Oct 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To activate the Wnt or BMP pathways, the cells were exposed to 0.5uM BIO or 25ng/ul rhBMP4, respectively, during the two hours in differentiation conditions.
| | Sample_growth_protocol_ch1 | CD34+ cells were expanded in Stemspan SFEM medium supplemented with 1XCC100 cytokine mix + 2% pen/strep. On day 6 of expansion, cells were transferred to erythroid differentiation medium (StemSpan SFEM Medium with 2% P/S, 20 ng/ml SCF, 1 U/ml Epo, 5 ng/ml IL-3, 2 uM dexamethasone, and 1 uM β-estradiol) for 2 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen was used for total RNA extraction and DNA removal.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA amplification and labeling was performed using GeneChip HT 3' IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, approximately 10 ug of cRNA were hybridized for 18 hr on Affymetrix 3' Expression GeneChip U133plus2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | Raw expression data was preprocessed by MAS5 and then log10 transformed. Biological replicates across arrays were then quantile normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Teresa,Venezia,Bowman
| | Sample_contact_laboratory | Zon Laboratory
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | One Blackfan Circle, 7th floor
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647156/suppl/GSM647156.CEL.gz
| | Sample_series_id | GSE26351
| | Sample_data_row_count | 54675
| | |
|
GSM647157 | GPL570 |
|
moPB_CD34_BMP4 treated biological rep 2
|
CD34 cells in erythroid differentiation medium for 2 hrs with 25ng/ul rhBMP4
|
cell type: moPB CD34+ selected cells
disease status: healthy normal volunteer
|
Gene expression data from CD34 cells in erythroid differentiation medium for 2 hrs with 25ng/ul rhBMP4
|
| Sample_geo_accession | GSM647157
| | Sample_status | Public on Oct 07 2011
| | Sample_submission_date | Dec 29 2010
| | Sample_last_update_date | Oct 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To activate the Wnt or BMP pathways, the cells were exposed to 0.5uM BIO or 25ng/ul rhBMP4, respectively, during the two hours in differentiation conditions.
| | Sample_growth_protocol_ch1 | CD34+ cells were expanded in Stemspan SFEM medium supplemented with 1XCC100 cytokine mix + 2% pen/strep. On day 6 of expansion, cells were transferred to erythroid differentiation medium (StemSpan SFEM Medium with 2% P/S, 20 ng/ml SCF, 1 U/ml Epo, 5 ng/ml IL-3, 2 uM dexamethasone, and 1 uM β-estradiol) for 2 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen was used for total RNA extraction and DNA removal.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA amplification and labeling was performed using GeneChip HT 3' IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, approximately 10 ug of cRNA were hybridized for 18 hr on Affymetrix 3' Expression GeneChip U133plus2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | Raw expression data was preprocessed by MAS5 and then log10 transformed. Biological replicates across arrays were then quantile normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Teresa,Venezia,Bowman
| | Sample_contact_laboratory | Zon Laboratory
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | One Blackfan Circle, 7th floor
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647157/suppl/GSM647157.CEL.gz
| | Sample_series_id | GSE26351
| | Sample_data_row_count | 54675
| | |
|
GSM647158 | GPL570 |
|
moPB_CD34_BMP4 treated biological rep 3
|
CD34 cells in erythroid differentiation medium for 2 hrs with 25ng/ul rhBMP4
|
cell type: moPB CD34+ selected cells
disease status: healthy normal volunteer
|
Gene expression data from CD34 cells in erythroid differentiation medium for 2 hrs with 25ng/ul rhBMP4
|
| Sample_geo_accession | GSM647158
| | Sample_status | Public on Oct 07 2011
| | Sample_submission_date | Dec 29 2010
| | Sample_last_update_date | Oct 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To activate the Wnt or BMP pathways, the cells were exposed to 0.5uM BIO or 25ng/ul rhBMP4, respectively, during the two hours in differentiation conditions.
| | Sample_growth_protocol_ch1 | CD34+ cells were expanded in Stemspan SFEM medium supplemented with 1XCC100 cytokine mix + 2% pen/strep. On day 6 of expansion, cells were transferred to erythroid differentiation medium (StemSpan SFEM Medium with 2% P/S, 20 ng/ml SCF, 1 U/ml Epo, 5 ng/ml IL-3, 2 uM dexamethasone, and 1 uM β-estradiol) for 2 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen was used for total RNA extraction and DNA removal.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA amplification and labeling was performed using GeneChip HT 3' IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, approximately 10 ug of cRNA were hybridized for 18 hr on Affymetrix 3' Expression GeneChip U133plus2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | Raw expression data was preprocessed by MAS5 and then log10 transformed. Biological replicates across arrays were then quantile normalized.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Teresa,Venezia,Bowman
| | Sample_contact_laboratory | Zon Laboratory
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | One Blackfan Circle, 7th floor
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647158/suppl/GSM647158.CEL.gz
| | Sample_series_id | GSE26351
| | Sample_data_row_count | 54675
| | |
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