Result

Search results for the GEO ID: GSE26393

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM647870

GPL1261

JN_RG

FACS sorted P4 early bulge stem cells from K14-RFP/Sox9-EGFP mice

tissue: skin strain: CD-1 genotype: K14-RFP/Sox9-EGFP age: P4

Gene expression data from FACS sorted P4 early bulge stem cells

Sample_geo_accession	GSM647870
Sample_status	Public on Jan 04 2011
Sample_submission_date	Jan 03 2011
Sample_last_update_date	Jan 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Hair follicle cells were isolated from P4 Backskin of K14-RFP/Sox9-EGFP double transgenic mice as following: interfollicular epidermis sheet was pealed from hair follicle & dermis after dispase treatment. The hair follicle & dermis were first digested by collagenase (Sigma). Intact hair follicles were separated from dermal cells by low speed spinning (20g). The hair follicles were then digested by Trypsin and filtered by 40 µm cell strainers. The isolated hair follicle cells were FACS sorted. Dead cells and large differentiated cells were excluded based on DAPI and side scattering. Early bulge cells were gated as GFPHi,RFPHi. Non-bulge ORS cells were gated as GFP-, RFPHi.
Sample_growth_protocol_ch1	Standard mouse growth protocol
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	miRNeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Labeled cRNA samples were hybridized to MOE 430 2.0 mouse expression arrays which were processed and analyzed according to standard Affymetrix protocols.
Sample_scan_protocol	Standard Affymetrix protocol
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix expression console default analysis settings
Sample_platform_id	GPL1261
Sample_contact_name	liang,,zhang
Sample_contact_email	liangz@colorado.edu
Sample_contact_institute	university of colorado at boulder
Sample_contact_address	University of Colorado at Boulder, Han lab, Department of MCD Biology, Campus box 0347
Sample_contact_city	Boulder
Sample_contact_state	CO
Sample_contact_zip/postal_code	80309-0347
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647870/suppl/GSM647870_JN_RG.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647870/suppl/GSM647870_JN_RG.CHP.gz
Sample_series_id	GSE26393
Sample_series_id	GSE26396
Sample_data_row_count	45101

GSM647871

GPL1261

JN_Rhi

FACS sorted P4 non-bulge ORS cells from K14-RFP/Sox9-EGFP mice

tissue: skin strain: CD-1 genotype: K14-RFP/Sox9-EGFP age: P4

Gene expression data from FACS sorted P4 non-bulge ORS cells

Sample_geo_accession	GSM647871
Sample_status	Public on Jan 04 2011
Sample_submission_date	Jan 03 2011
Sample_last_update_date	Jan 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Hair follicle cells were isolated from P4 Backskin of K14-RFP/Sox9-EGFP double transgenic mice as following: interfollicular epidermis sheet was pealed from hair follicle & dermis after dispase treatment. The hair follicle & dermis were first digested by collagenase (Sigma). Intact hair follicles were separated from dermal cells by low speed spinning (20g). The hair follicles were then digested by Trypsin and filtered by 40 µm cell strainers. The isolated hair follicle cells were FACS sorted. Dead cells and large differentiated cells were excluded based on DAPI and side scattering. Early bulge cells were gated as GFPHi,RFPHi. Non-bulge ORS cells were gated as GFP-, RFPHi.
Sample_growth_protocol_ch1	Standard mouse growth protocol
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	miRNeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Labeled cRNA samples were hybridized to MOE 430 2.0 mouse expression arrays which were processed and analyzed according to standard Affymetrix protocols.
Sample_scan_protocol	Standard Affymetrix protocol
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix expression console default analysis settings
Sample_platform_id	GPL1261
Sample_contact_name	liang,,zhang
Sample_contact_email	liangz@colorado.edu
Sample_contact_institute	university of colorado at boulder
Sample_contact_address	University of Colorado at Boulder, Han lab, Department of MCD Biology, Campus box 0347
Sample_contact_city	Boulder
Sample_contact_state	CO
Sample_contact_zip/postal_code	80309-0347
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647871/suppl/GSM647871_JN_RHi.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647871/suppl/GSM647871_JN_RHi.CHP.gz
Sample_series_id	GSE26393
Sample_series_id	GSE26396
Sample_data_row_count	45101

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