Search results for the GEO ID: GSE26394 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM647872 | GPL1261 |
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Male2_DTG_log2
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FACS sorted P4 ORS cells
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tissue: skin
strain: FVB/N
genotype: K14-rtTA/TRE-miR-125b/K14-H2BGFP
age: P4
Sex: male
treatment: Dox 24hrs
|
Gene expression data from FACS sorted P4 ORS
|
Sample_geo_accession | GSM647872
| Sample_status | Public on Jan 04 2011
| Sample_submission_date | Jan 03 2011
| Sample_last_update_date | Jan 04 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Hair follicle cells were isolated from P4 Backskin (Dox since P3 for 24hrs) of DTG (K14-rtTA/TRE-miR-125b/K14-H2BGFP), TRE (TRE-miR-125b/K14-H2BGFP), KrtA (K14-rtTA/K14-H2BGFP) as following: interfollicular epidermis sheet was pealed from hair follicle & dermis after dispase treatment.The hair follicle & dermis were first digested by collagenase (Sigma). Intact hair follicles were separated from dermal cells by low speed spinning (20g). The hair follicles were then digested by Trypsin and filtered by 40 µm cell strainers. The isolated hair follicle cells were FACS sorted. During FACS, cells were first gated against CD34 (endothelial cells), CD45 (immune cells), CD114 (melanocytes) and DAPI (dead cells). ORS cells were sorted from the remaining cells as α6HiGFPHi
| Sample_growth_protocol_ch1 | Standard mouse growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | miRNeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Labeled cRNA samples were hybridized to MOE 430 2.0 mouse expression arrays which were processed and analyzed according to standard Affymetrix protocols.
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | The data were analyzed with RMA using Affymetrix expression console default analysis settings. All displayed signals were log2 transformed
| Sample_platform_id | GPL1261
| Sample_contact_name | liang,,zhang
| Sample_contact_email | liangz@colorado.edu
| Sample_contact_institute | university of colorado at boulder
| Sample_contact_address | University of Colorado at Boulder, Han lab, Department of MCD Biology, Campus box 0347
| Sample_contact_city | Boulder
| Sample_contact_state | CO
| Sample_contact_zip/postal_code | 80309-0347
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647872/suppl/GSM647872_LZ_Male2_DTG.CEL.gz
| Sample_series_id | GSE26394
| Sample_series_id | GSE26396
| Sample_data_row_count | 45101
| |
|
GSM647873 | GPL1261 |
|
Male8_TRE_log2
|
FACS sorted P4 ORS cells
|
tissue: skin
strain: FVB/N
genotype: TRE-miR-125b/K14-H2BGFP
age: P4
Sex: male
treatment: Dox 24hrs
|
Gene expression data from FACS sorted P4 ORS
|
Sample_geo_accession | GSM647873
| Sample_status | Public on Jan 04 2011
| Sample_submission_date | Jan 03 2011
| Sample_last_update_date | Jan 04 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Hair follicle cells were isolated from P4 Backskin (Dox since P3 for 24hrs) of DTG (K14-rtTA/TRE-miR-125b/K14-H2BGFP), TRE (TRE-miR-125b/K14-H2BGFP), KrtA (K14-rtTA/K14-H2BGFP) as following: interfollicular epidermis sheet was pealed from hair follicle & dermis after dispase treatment.The hair follicle & dermis were first digested by collagenase (Sigma). Intact hair follicles were separated from dermal cells by low speed spinning (20g). The hair follicles were then digested by Trypsin and filtered by 40 µm cell strainers. The isolated hair follicle cells were FACS sorted. During FACS, cells were first gated against CD34 (endothelial cells), CD45 (immune cells), CD114 (melanocytes) and DAPI (dead cells). ORS cells were sorted from the remaining cells as α6HiGFPHi
| Sample_growth_protocol_ch1 | Standard mouse growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | miRNeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Labeled cRNA samples were hybridized to MOE 430 2.0 mouse expression arrays which were processed and analyzed according to standard Affymetrix protocols.
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | The data were analyzed with RMA using Affymetrix expression console default analysis settings. All displayed signals were log2 transformed
| Sample_platform_id | GPL1261
| Sample_contact_name | liang,,zhang
| Sample_contact_email | liangz@colorado.edu
| Sample_contact_institute | university of colorado at boulder
| Sample_contact_address | University of Colorado at Boulder, Han lab, Department of MCD Biology, Campus box 0347
| Sample_contact_city | Boulder
| Sample_contact_state | CO
| Sample_contact_zip/postal_code | 80309-0347
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647873/suppl/GSM647873_LZ_Male8_TRE.CEL.gz
| Sample_series_id | GSE26394
| Sample_series_id | GSE26396
| Sample_data_row_count | 45101
| |
|
GSM647874 | GPL1261 |
|
Female2_KrtA_log2
|
FACS sorted P4 ORS cells
|
tissue: skin
strain: FVB/N
genotype: K14-rtTA/K14-H2BGFP
age: P4
Sex: female
treatment: Dox 24hrs
|
Gene expression data from FACS sorted P4 ORS
|
Sample_geo_accession | GSM647874
| Sample_status | Public on Jan 04 2011
| Sample_submission_date | Jan 03 2011
| Sample_last_update_date | Jan 04 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Hair follicle cells were isolated from P4 Backskin (Dox since P3 for 24hrs) of DTG (K14-rtTA/TRE-miR-125b/K14-H2BGFP), TRE (TRE-miR-125b/K14-H2BGFP), KrtA (K14-rtTA/K14-H2BGFP) as following: interfollicular epidermis sheet was pealed from hair follicle & dermis after dispase treatment.The hair follicle & dermis were first digested by collagenase (Sigma). Intact hair follicles were separated from dermal cells by low speed spinning (20g). The hair follicles were then digested by Trypsin and filtered by 40 µm cell strainers. The isolated hair follicle cells were FACS sorted. During FACS, cells were first gated against CD34 (endothelial cells), CD45 (immune cells), CD114 (melanocytes) and DAPI (dead cells). ORS cells were sorted from the remaining cells as α6HiGFPHi
| Sample_growth_protocol_ch1 | Standard mouse growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | miRNeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Labeled cRNA samples were hybridized to MOE 430 2.0 mouse expression arrays which were processed and analyzed according to standard Affymetrix protocols.
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | The data were analyzed with RMA using Affymetrix expression console default analysis settings. All displayed signals were log2 transformed
| Sample_platform_id | GPL1261
| Sample_contact_name | liang,,zhang
| Sample_contact_email | liangz@colorado.edu
| Sample_contact_institute | university of colorado at boulder
| Sample_contact_address | University of Colorado at Boulder, Han lab, Department of MCD Biology, Campus box 0347
| Sample_contact_city | Boulder
| Sample_contact_state | CO
| Sample_contact_zip/postal_code | 80309-0347
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647874/suppl/GSM647874_LZ_Female2_KrtA.CEL.gz
| Sample_series_id | GSE26394
| Sample_series_id | GSE26396
| Sample_data_row_count | 45101
| |
|
GSM647875 | GPL1261 |
|
Female10_DTG_log2
|
FACS sorted P4 ORS cells
|
tissue: skin
strain: FVB/N
genotype: K14-rtTA/TRE-miR-125b/K14-H2BGFP
age: P4
Sex: female
treatment: Dox 24hrs
|
Gene expression data from FACS sorted P4 ORS
|
Sample_geo_accession | GSM647875
| Sample_status | Public on Jan 04 2011
| Sample_submission_date | Jan 03 2011
| Sample_last_update_date | Jan 04 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Hair follicle cells were isolated from P4 Backskin (Dox since P3 for 24hrs) of DTG (K14-rtTA/TRE-miR-125b/K14-H2BGFP), TRE (TRE-miR-125b/K14-H2BGFP), KrtA (K14-rtTA/K14-H2BGFP) as following: interfollicular epidermis sheet was pealed from hair follicle & dermis after dispase treatment.The hair follicle & dermis were first digested by collagenase (Sigma). Intact hair follicles were separated from dermal cells by low speed spinning (20g). The hair follicles were then digested by Trypsin and filtered by 40 µm cell strainers. The isolated hair follicle cells were FACS sorted. During FACS, cells were first gated against CD34 (endothelial cells), CD45 (immune cells), CD114 (melanocytes) and DAPI (dead cells). ORS cells were sorted from the remaining cells as α6HiGFPHi
| Sample_growth_protocol_ch1 | Standard mouse growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | miRNeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Labeled cRNA samples were hybridized to MOE 430 2.0 mouse expression arrays which were processed and analyzed according to standard Affymetrix protocols.
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | The data were analyzed with RMA using Affymetrix expression console default analysis settings. All displayed signals were log2 transformed
| Sample_platform_id | GPL1261
| Sample_contact_name | liang,,zhang
| Sample_contact_email | liangz@colorado.edu
| Sample_contact_institute | university of colorado at boulder
| Sample_contact_address | University of Colorado at Boulder, Han lab, Department of MCD Biology, Campus box 0347
| Sample_contact_city | Boulder
| Sample_contact_state | CO
| Sample_contact_zip/postal_code | 80309-0347
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM647nnn/GSM647875/suppl/GSM647875_LZ_Female10_DTG.CEL.gz
| Sample_series_id | GSE26394
| Sample_series_id | GSE26396
| Sample_data_row_count | 45101
| |
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