Search results for the GEO ID: GSE26455  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM649319 | GPL570 |
|
ES4SKIN, biological rep 1
|
Trancripts from ESIMR90
|
cell type: iPS cells derived from FORESKIN
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ES4SKIN_1
|
| Sample_geo_accession | GSM649319
| | Sample_status | Public on Jan 05 2011
| | Sample_submission_date | Jan 05 2011
| | Sample_last_update_date | Jan 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | HES-3 and ES4SKIN were cultured on BD Matrigel in mTeSR1 media; FORESKIN was cultured in DMEM media supplemented with 10% FBS, 2mM L-glutamine, and 20 units each of penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cell pellets using TRIzol reagent (Invitrogen) as per manufacturer's protocol. RNA extracted into the aqueous phase was concentrated by precipitation in equal volume of isopropnaol at -20°C overnight, and subsequently purified using Qiagen Rneasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was reversed transcribed and biotinylated using Affymetrix 3' IVT Express Kit.
| | Sample_hyb_protocol | Hybridisations were carried out at 45°C for 16h with reagents from Affymetrix “GeneChip Hybridization, Wash, and Stain Kit”, with subsequent washing and staining of arrays automated and preprogrammed (FS450_0001) on GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using GeneChip Scanner 3000 using default settings.
| | Sample_data_processing | Images were extracted and analyzed by MAS5.0 workflow with default settings in Expression Console v1.1.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yee Jiun,,Kok
| | Sample_contact_email | kok_yee_jiun@bti.a-star.edu.sg
| | Sample_contact_institute | Bioprocessing Technology Institute
| | Sample_contact_address | 20 Biopolis Way, #06-01 Centros
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138668
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM649nnn/GSM649319/suppl/GSM649319.CEL.gz
| | Sample_series_id | GSE26451
| | Sample_series_id | GSE26455
| | Sample_data_row_count | 54675
| | |
|
GSM649320 | GPL570 |
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ES4SKIN, biological rep 2
|
Trancripts from ESIMR90
|
cell type: iPS cells derived from FORESKIN
|
ES4SKIN_2
|
| Sample_geo_accession | GSM649320
| | Sample_status | Public on Jan 05 2011
| | Sample_submission_date | Jan 05 2011
| | Sample_last_update_date | Jan 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | HES-3 and ES4SKIN were cultured on BD Matrigel in mTeSR1 media; FORESKIN was cultured in DMEM media supplemented with 10% FBS, 2mM L-glutamine, and 20 units each of penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cell pellets using TRIzol reagent (Invitrogen) as per manufacturer's protocol. RNA extracted into the aqueous phase was concentrated by precipitation in equal volume of isopropnaol at -20°C overnight, and subsequently purified using Qiagen Rneasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was reversed transcribed and biotinylated using Affymetrix 3' IVT Express Kit.
| | Sample_hyb_protocol | Hybridisations were carried out at 45°C for 16h with reagents from Affymetrix “GeneChip Hybridization, Wash, and Stain Kit”, with subsequent washing and staining of arrays automated and preprogrammed (FS450_0001) on GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using GeneChip Scanner 3000 using default settings.
| | Sample_data_processing | Images were extracted and analyzed by MAS5.0 workflow with default settings in Expression Console v1.1.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yee Jiun,,Kok
| | Sample_contact_email | kok_yee_jiun@bti.a-star.edu.sg
| | Sample_contact_institute | Bioprocessing Technology Institute
| | Sample_contact_address | 20 Biopolis Way, #06-01 Centros
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138668
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM649nnn/GSM649320/suppl/GSM649320.CEL.gz
| | Sample_series_id | GSE26451
| | Sample_series_id | GSE26455
| | Sample_data_row_count | 54675
| | |
|
GSM649321 | GPL570 |
|
HES-3, biological rep 1
|
Trancripts from HES-3
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cell type: embryonic stem cells from ESI
|
HES-3_1
|
| Sample_geo_accession | GSM649321
| | Sample_status | Public on Jan 05 2011
| | Sample_submission_date | Jan 05 2011
| | Sample_last_update_date | Jan 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | HES-3 and ES4SKIN were cultured on BD Matrigel in mTeSR1 media; FORESKIN was cultured in DMEM media supplemented with 10% FBS, 2mM L-glutamine, and 20 units each of penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cell pellets using TRIzol reagent (Invitrogen) as per manufacturer's protocol. RNA extracted into the aqueous phase was concentrated by precipitation in equal volume of isopropnaol at -20°C overnight, and subsequently purified using Qiagen Rneasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was reversed transcribed and biotinylated using Affymetrix 3' IVT Express Kit.
| | Sample_hyb_protocol | Hybridisations were carried out at 45°C for 16h with reagents from Affymetrix “GeneChip Hybridization, Wash, and Stain Kit”, with subsequent washing and staining of arrays automated and preprogrammed (FS450_0001) on GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using GeneChip Scanner 3000 using default settings.
| | Sample_data_processing | Images were extracted and analyzed by MAS5.0 workflow with default settings in Expression Console v1.1.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yee Jiun,,Kok
| | Sample_contact_email | kok_yee_jiun@bti.a-star.edu.sg
| | Sample_contact_institute | Bioprocessing Technology Institute
| | Sample_contact_address | 20 Biopolis Way, #06-01 Centros
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138668
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM649nnn/GSM649321/suppl/GSM649321.CEL.gz
| | Sample_series_id | GSE26451
| | Sample_series_id | GSE26455
| | Sample_data_row_count | 54675
| | |
|
GSM649322 | GPL570 |
|
HES-3, biological rep 2
|
Trancripts from HES-3
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cell type: embryonic stem cells from ESI
|
HES-3_2
|
| Sample_geo_accession | GSM649322
| | Sample_status | Public on Jan 05 2011
| | Sample_submission_date | Jan 05 2011
| | Sample_last_update_date | Jan 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | HES-3 and ES4SKIN were cultured on BD Matrigel in mTeSR1 media; FORESKIN was cultured in DMEM media supplemented with 10% FBS, 2mM L-glutamine, and 20 units each of penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cell pellets using TRIzol reagent (Invitrogen) as per manufacturer's protocol. RNA extracted into the aqueous phase was concentrated by precipitation in equal volume of isopropnaol at -20°C overnight, and subsequently purified using Qiagen Rneasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was reversed transcribed and biotinylated using Affymetrix 3' IVT Express Kit.
| | Sample_hyb_protocol | Hybridisations were carried out at 45°C for 16h with reagents from Affymetrix “GeneChip Hybridization, Wash, and Stain Kit”, with subsequent washing and staining of arrays automated and preprogrammed (FS450_0001) on GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using GeneChip Scanner 3000 using default settings.
| | Sample_data_processing | Images were extracted and analyzed by MAS5.0 workflow with default settings in Expression Console v1.1.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yee Jiun,,Kok
| | Sample_contact_email | kok_yee_jiun@bti.a-star.edu.sg
| | Sample_contact_institute | Bioprocessing Technology Institute
| | Sample_contact_address | 20 Biopolis Way, #06-01 Centros
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138668
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM649nnn/GSM649322/suppl/GSM649322.CEL.gz
| | Sample_series_id | GSE26451
| | Sample_series_id | GSE26455
| | Sample_data_row_count | 54675
| | |
|
GSM649323 | GPL570 |
|
FORESKIN, biological rep 1
|
Trancripts from FORESKIN
|
cell type: newborn foreskin fibroblasts from ATCC (CRL-2097)
|
FORESKIN_1
|
| Sample_geo_accession | GSM649323
| | Sample_status | Public on Jan 05 2011
| | Sample_submission_date | Jan 05 2011
| | Sample_last_update_date | Jan 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | HES-3 and ES4SKIN were cultured on BD Matrigel in mTeSR1 media; FORESKIN was cultured in DMEM media supplemented with 10% FBS, 2mM L-glutamine, and 20 units each of penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cell pellets using TRIzol reagent (Invitrogen) as per manufacturer's protocol. RNA extracted into the aqueous phase was concentrated by precipitation in equal volume of isopropnaol at -20°C overnight, and subsequently purified using Qiagen Rneasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was reversed transcribed and biotinylated using Affymetrix 3' IVT Express Kit.
| | Sample_hyb_protocol | Hybridisations were carried out at 45°C for 16h with reagents from Affymetrix “GeneChip Hybridization, Wash, and Stain Kit”, with subsequent washing and staining of arrays automated and preprogrammed (FS450_0001) on GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using GeneChip Scanner 3000 using default settings.
| | Sample_data_processing | Images were extracted and analyzed by MAS5.0 workflow with default settings in Expression Console v1.1.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yee Jiun,,Kok
| | Sample_contact_email | kok_yee_jiun@bti.a-star.edu.sg
| | Sample_contact_institute | Bioprocessing Technology Institute
| | Sample_contact_address | 20 Biopolis Way, #06-01 Centros
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138668
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM649nnn/GSM649323/suppl/GSM649323.CEL.gz
| | Sample_series_id | GSE26451
| | Sample_series_id | GSE26455
| | Sample_data_row_count | 54675
| | |
|
GSM649324 | GPL570 |
|
FORESKIN, biological rep 2
|
Trancripts from FORESKIN
|
cell type: newborn foreskin fibroblasts from ATCC (CRL-2097)
|
FORESKIN_2
|
| Sample_geo_accession | GSM649324
| | Sample_status | Public on Jan 05 2011
| | Sample_submission_date | Jan 05 2011
| | Sample_last_update_date | Jan 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | HES-3 and ES4SKIN were cultured on BD Matrigel in mTeSR1 media; FORESKIN was cultured in DMEM media supplemented with 10% FBS, 2mM L-glutamine, and 20 units each of penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cell pellets using TRIzol reagent (Invitrogen) as per manufacturer's protocol. RNA extracted into the aqueous phase was concentrated by precipitation in equal volume of isopropnaol at -20°C overnight, and subsequently purified using Qiagen Rneasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was reversed transcribed and biotinylated using Affymetrix 3' IVT Express Kit.
| | Sample_hyb_protocol | Hybridisations were carried out at 45°C for 16h with reagents from Affymetrix “GeneChip Hybridization, Wash, and Stain Kit”, with subsequent washing and staining of arrays automated and preprogrammed (FS450_0001) on GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using GeneChip Scanner 3000 using default settings.
| | Sample_data_processing | Images were extracted and analyzed by MAS5.0 workflow with default settings in Expression Console v1.1.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yee Jiun,,Kok
| | Sample_contact_email | kok_yee_jiun@bti.a-star.edu.sg
| | Sample_contact_institute | Bioprocessing Technology Institute
| | Sample_contact_address | 20 Biopolis Way, #06-01 Centros
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138668
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM649nnn/GSM649324/suppl/GSM649324.CEL.gz
| | Sample_series_id | GSE26451
| | Sample_series_id | GSE26455
| | Sample_data_row_count | 54675
| | |
|
GSM649332 | GPL570 |
|
ESIMR90, biological rep1
|
Trancripts from ESIMR90
|
cell type: iPS cells derived from IMR90
|
ESIMR90_1
|
| Sample_geo_accession | GSM649332
| | Sample_status | Public on Jan 05 2011
| | Sample_submission_date | Jan 05 2011
| | Sample_last_update_date | Jan 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | HES-3 and ESIMR90 were cultured on BD Matrigel in mTeSR1 media; IMR90 was cultured in DMEM media supplemented with 10% FBS, 2mM L-glutamine, and 20 units each of penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cell pellets using TRIzol reagent (Invitrogen) as per manufacturer's protocol. RNA extracted into the aqueous phase was concentrated by precipitation in equal volume of isopropnaol at -20°C overnight, and subsequently purified using Qiagen Rneasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was reversed transcribed and biotinylated using Affymetrix 3' IVT Express Kit.
| | Sample_hyb_protocol | Hybridisations were carried out at 45°C for 16h with reagents from Affymetrix “GeneChip Hybridization, Wash, and Stain Kit”, with subsequent washing and staining of arrays automated and preprogrammed (FS450_0001) on GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using GeneChip Scanner 3000 using default settings.
| | Sample_data_processing | Images were extracted and analyzed by MAS5.0 workflow with default settings in Expression Console v1.1.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yee Jiun,,Kok
| | Sample_contact_email | kok_yee_jiun@bti.a-star.edu.sg
| | Sample_contact_institute | Bioprocessing Technology Institute
| | Sample_contact_address | 20 Biopolis Way, #06-01 Centros
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138668
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM649nnn/GSM649332/suppl/GSM649332.CEL.gz
| | Sample_series_id | GSE26453
| | Sample_series_id | GSE26455
| | Sample_data_row_count | 54675
| | |
|
GSM649333 | GPL570 |
|
ESIMR90, biological rep2
|
Trancripts from ESIMR90
|
cell type: iPS cells derived from IMR90
|
ESIMR90_2
|
| Sample_geo_accession | GSM649333
| | Sample_status | Public on Jan 05 2011
| | Sample_submission_date | Jan 05 2011
| | Sample_last_update_date | Jan 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | HES-3 and ESIMR90 were cultured on BD Matrigel in mTeSR1 media; IMR90 was cultured in DMEM media supplemented with 10% FBS, 2mM L-glutamine, and 20 units each of penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cell pellets using TRIzol reagent (Invitrogen) as per manufacturer's protocol. RNA extracted into the aqueous phase was concentrated by precipitation in equal volume of isopropnaol at -20°C overnight, and subsequently purified using Qiagen Rneasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was reversed transcribed and biotinylated using Affymetrix 3' IVT Express Kit.
| | Sample_hyb_protocol | Hybridisations were carried out at 45°C for 16h with reagents from Affymetrix “GeneChip Hybridization, Wash, and Stain Kit”, with subsequent washing and staining of arrays automated and preprogrammed (FS450_0001) on GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using GeneChip Scanner 3000 using default settings.
| | Sample_data_processing | Images were extracted and analyzed by MAS5.0 workflow with default settings in Expression Console v1.1.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yee Jiun,,Kok
| | Sample_contact_email | kok_yee_jiun@bti.a-star.edu.sg
| | Sample_contact_institute | Bioprocessing Technology Institute
| | Sample_contact_address | 20 Biopolis Way, #06-01 Centros
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138668
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM649nnn/GSM649333/suppl/GSM649333.CEL.gz
| | Sample_series_id | GSE26453
| | Sample_series_id | GSE26455
| | Sample_data_row_count | 54675
| | |
|
GSM649334 | GPL570 |
|
HES-3, biological rep1
|
Trancripts from HES-3
|
cell type: embryonic stem cells from ESI
|
HES-3_1
|
| Sample_geo_accession | GSM649334
| | Sample_status | Public on Jan 05 2011
| | Sample_submission_date | Jan 05 2011
| | Sample_last_update_date | Jan 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | HES-3 and ESIMR90 were cultured on BD Matrigel in mTeSR1 media; IMR90 was cultured in DMEM media supplemented with 10% FBS, 2mM L-glutamine, and 20 units each of penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cell pellets using TRIzol reagent (Invitrogen) as per manufacturer's protocol. RNA extracted into the aqueous phase was concentrated by precipitation in equal volume of isopropnaol at -20°C overnight, and subsequently purified using Qiagen Rneasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was reversed transcribed and biotinylated using Affymetrix 3' IVT Express Kit.
| | Sample_hyb_protocol | Hybridisations were carried out at 45°C for 16h with reagents from Affymetrix “GeneChip Hybridization, Wash, and Stain Kit”, with subsequent washing and staining of arrays automated and preprogrammed (FS450_0001) on GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using GeneChip Scanner 3000 using default settings.
| | Sample_data_processing | Images were extracted and analyzed by MAS5.0 workflow with default settings in Expression Console v1.1.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yee Jiun,,Kok
| | Sample_contact_email | kok_yee_jiun@bti.a-star.edu.sg
| | Sample_contact_institute | Bioprocessing Technology Institute
| | Sample_contact_address | 20 Biopolis Way, #06-01 Centros
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138668
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM649nnn/GSM649334/suppl/GSM649334.CEL.gz
| | Sample_series_id | GSE26453
| | Sample_series_id | GSE26455
| | Sample_data_row_count | 54675
| | |
|
GSM649335 | GPL570 |
|
HES-3, biological rep2
|
Trancripts from HES-3
|
cell type: embryonic stem cells from ESI
|
HES-3_2
|
| Sample_geo_accession | GSM649335
| | Sample_status | Public on Jan 05 2011
| | Sample_submission_date | Jan 05 2011
| | Sample_last_update_date | Jan 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | HES-3 and ESIMR90 were cultured on BD Matrigel in mTeSR1 media; IMR90 was cultured in DMEM media supplemented with 10% FBS, 2mM L-glutamine, and 20 units each of penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cell pellets using TRIzol reagent (Invitrogen) as per manufacturer's protocol. RNA extracted into the aqueous phase was concentrated by precipitation in equal volume of isopropnaol at -20°C overnight, and subsequently purified using Qiagen Rneasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was reversed transcribed and biotinylated using Affymetrix 3' IVT Express Kit.
| | Sample_hyb_protocol | Hybridisations were carried out at 45°C for 16h with reagents from Affymetrix “GeneChip Hybridization, Wash, and Stain Kit”, with subsequent washing and staining of arrays automated and preprogrammed (FS450_0001) on GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using GeneChip Scanner 3000 using default settings.
| | Sample_data_processing | Images were extracted and analyzed by MAS5.0 workflow with default settings in Expression Console v1.1.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yee Jiun,,Kok
| | Sample_contact_email | kok_yee_jiun@bti.a-star.edu.sg
| | Sample_contact_institute | Bioprocessing Technology Institute
| | Sample_contact_address | 20 Biopolis Way, #06-01 Centros
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138668
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM649nnn/GSM649335/suppl/GSM649335.CEL.gz
| | Sample_series_id | GSE26453
| | Sample_series_id | GSE26455
| | Sample_data_row_count | 54675
| | |
|
GSM649336 | GPL570 |
|
IMR90, biological rep1
|
Trancripts from IMR90
|
cell type: female fetal lung fibroblasts from ATCC (CCL-186)
|
IMR90_1
|
| Sample_geo_accession | GSM649336
| | Sample_status | Public on Jan 05 2011
| | Sample_submission_date | Jan 05 2011
| | Sample_last_update_date | Jan 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | HES-3 and ESIMR90 were cultured on BD Matrigel in mTeSR1 media; IMR90 was cultured in DMEM media supplemented with 10% FBS, 2mM L-glutamine, and 20 units each of penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cell pellets using TRIzol reagent (Invitrogen) as per manufacturer's protocol. RNA extracted into the aqueous phase was concentrated by precipitation in equal volume of isopropnaol at -20°C overnight, and subsequently purified using Qiagen Rneasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was reversed transcribed and biotinylated using Affymetrix 3' IVT Express Kit.
| | Sample_hyb_protocol | Hybridisations were carried out at 45°C for 16h with reagents from Affymetrix “GeneChip Hybridization, Wash, and Stain Kit”, with subsequent washing and staining of arrays automated and preprogrammed (FS450_0001) on GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using GeneChip Scanner 3000 using default settings.
| | Sample_data_processing | Images were extracted and analyzed by MAS5.0 workflow with default settings in Expression Console v1.1.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yee Jiun,,Kok
| | Sample_contact_email | kok_yee_jiun@bti.a-star.edu.sg
| | Sample_contact_institute | Bioprocessing Technology Institute
| | Sample_contact_address | 20 Biopolis Way, #06-01 Centros
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138668
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM649nnn/GSM649336/suppl/GSM649336.CEL.gz
| | Sample_series_id | GSE26453
| | Sample_series_id | GSE26455
| | Sample_data_row_count | 54675
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GSM649337 | GPL570 |
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IMR90, biological rep2
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Trancripts from IMR90
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cell type: female fetal lung fibroblasts from ATCC (CCL-186)
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IMR90_2
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| Sample_geo_accession | GSM649337
| | Sample_status | Public on Jan 05 2011
| | Sample_submission_date | Jan 05 2011
| | Sample_last_update_date | Jan 05 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | HES-3 and ESIMR90 were cultured on BD Matrigel in mTeSR1 media; IMR90 was cultured in DMEM media supplemented with 10% FBS, 2mM L-glutamine, and 20 units each of penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cell pellets using TRIzol reagent (Invitrogen) as per manufacturer's protocol. RNA extracted into the aqueous phase was concentrated by precipitation in equal volume of isopropnaol at -20°C overnight, and subsequently purified using Qiagen Rneasy Mini Kit according to manufacturer's protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was reversed transcribed and biotinylated using Affymetrix 3' IVT Express Kit.
| | Sample_hyb_protocol | Hybridisations were carried out at 45°C for 16h with reagents from Affymetrix “GeneChip Hybridization, Wash, and Stain Kit”, with subsequent washing and staining of arrays automated and preprogrammed (FS450_0001) on GeneChip Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using GeneChip Scanner 3000 using default settings.
| | Sample_data_processing | Images were extracted and analyzed by MAS5.0 workflow with default settings in Expression Console v1.1.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yee Jiun,,Kok
| | Sample_contact_email | kok_yee_jiun@bti.a-star.edu.sg
| | Sample_contact_institute | Bioprocessing Technology Institute
| | Sample_contact_address | 20 Biopolis Way, #06-01 Centros
| | Sample_contact_city | Singapore
| | Sample_contact_zip/postal_code | 138668
| | Sample_contact_country | Singapore
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM649nnn/GSM649337/suppl/GSM649337.CEL.gz
| | Sample_series_id | GSE26453
| | Sample_series_id | GSE26455
| | Sample_data_row_count | 54675
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