Search results for the GEO ID: GSE26495  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM651411 | GPL570 |
|
Naive CD8+ CD3+ T cells - rep1
|
Peripheral blood cells healthy adults
|
cell phenotype: CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651411
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651411/suppl/GSM651411.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651411/suppl/GSM651411.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651412 | GPL570 |
|
Naive CD8+ CD3+ T cells - rep2
|
Peripheral blood cells healthy adults
|
cell phenotype: CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651412
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651412/suppl/GSM651412.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651412/suppl/GSM651412.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651413 | GPL570 |
|
Naive CD8+ CD3+ T cells - rep3
|
Peripheral blood cells healthy adults
|
cell phenotype: CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651413
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651413/suppl/GSM651413.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651413/suppl/GSM651413.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651415 | GPL570 |
|
Naive CD8+ CD3+ T cells - rep4
|
Peripheral blood cells healthy adults
|
cell phenotype: CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651415
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651415/suppl/GSM651415.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651415/suppl/GSM651415.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651416 | GPL570 |
|
PD-1 high CD8+ CD3+ T cells - rep1
|
Peripheral blood cells healthy adults
|
cell phenotype: PD-1 high CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651416
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651416/suppl/GSM651416.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651416/suppl/GSM651416.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651417 | GPL570 |
|
PD-1 high CD8+ CD3+ T cells - rep2
|
Peripheral blood cells healthy adults
|
cell phenotype: PD-1 high CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651417
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651417/suppl/GSM651417.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651417/suppl/GSM651417.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651418 | GPL570 |
|
PD-1 high CD8+ CD3+ T cells - rep3
|
Peripheral blood cells healthy adults
|
cell phenotype: PD-1 high CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651418
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651418/suppl/GSM651418.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651418/suppl/GSM651418.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651419 | GPL570 |
|
PD-1 high CD8+ CD3+ T cells - rep4
|
Peripheral blood cells healthy adults
|
cell phenotype: PD-1 high CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651419
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651419/suppl/GSM651419.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651419/suppl/GSM651419.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651420 | GPL570 |
|
PD-1 high CD8+ CD3+ T cells - rep5
|
Peripheral blood cells healthy adults
|
cell phenotype: PD-1 high CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651420
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651420/suppl/GSM651420.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651420/suppl/GSM651420.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651422 | GPL570 |
|
PD-1 high CD8+ CD3+ T cells - rep6
|
Peripheral blood cells healthy adults
|
cell phenotype: PD-1 high CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651422
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651422/suppl/GSM651422.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651422/suppl/GSM651422.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651423 | GPL570 |
|
PD-1 low CD8+ CD3+ T cells - rep1
|
Peripheral blood cells healthy adults
|
cell phenotype: PD-1 low CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651423
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651423/suppl/GSM651423.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651423/suppl/GSM651423.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651425 | GPL570 |
|
PD-1 low CD8+ CD3+ T cells - rep2
|
Peripheral blood cells healthy adults
|
cell phenotype: PD-1 low CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651425
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651425/suppl/GSM651425.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651425/suppl/GSM651425.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651426 | GPL570 |
|
PD-1 low CD8+ CD3+ T cells - rep3
|
Peripheral blood cells healthy adults
|
cell phenotype: PD-1 low CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651426
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651426/suppl/GSM651426.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651426/suppl/GSM651426.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651427 | GPL570 |
|
PD-1 low CD8+ CD3+ T cells - rep4
|
Peripheral blood cells healthy adults
|
cell phenotype: PD-1 low CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651427
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651427/suppl/GSM651427.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651427/suppl/GSM651427.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651429 | GPL570 |
|
PD-1 low CD8+ CD3+ T cells - rep5
|
Peripheral blood cells healthy adults
|
cell phenotype: PD-1 low CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651429
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651429/suppl/GSM651429.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651429/suppl/GSM651429.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
GSM651430 | GPL570 |
|
PD-1 low CD8+ CD3+ T cells - rep6
|
Peripheral blood cells healthy adults
|
cell phenotype: PD-1 low CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
|
| Sample_geo_accession | GSM651430
| | Sample_status | Public on Jan 11 2011
| | Sample_submission_date | Jan 07 2011
| | Sample_last_update_date | Jan 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
| | Sample_hyb_protocol | Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
| | Sample_scan_protocol | Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
| | Sample_data_processing | Gene expression data was summarized with RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jaikumar ,,Duraiswamy
| | Sample_contact_email | jaikumar@mail.med.upenn.edu
| | Sample_contact_laboratory | Ahmed lab
| | Sample_contact_department | Emory Vaccine Center and Department of Microbiology & Immunology
| | Sample_contact_institute | Emory Univesity
| | Sample_contact_address | 1510, Clifton Road, G211, Rollins Research Center
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651430/suppl/GSM651430.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM651nnn/GSM651430/suppl/GSM651430.chp.gz
| | Sample_series_id | GSE26495
| | Sample_data_row_count | 54675
| | |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
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