Search results for the GEO ID: GSE26568  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM653199 | GPL1261 |
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evGFP transduced CTL, biological rep. 1
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In vitro differentiated CTL, day 4
|
tissue: In vitro cell culture
strain: C57BL/6
genotype: P14 LCMV TCR transgenic
age: 8-12 week old
|
Gene expression data from control CTL
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| Sample_geo_accession | GSM653199
| | Sample_status | Public on May 31 2013
| | Sample_submission_date | Jan 11 2011
| | Sample_last_update_date | May 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Retroviral transduction of isolated T cells was performed at 18 hours after peptide stimulation
| | Sample_growth_protocol_ch1 | Splenocytes were activated for 48 hours with gp33-41 peptide, then washed out and cultured a further 48 hours in IL-2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Minikit (Qiagen) according to manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3’ IVT Express Kit from 250ng total RNA
| | Sample_hyb_protocol | Each sample was hybridized to GeneChip® Mouse Genome 430 2.0 Array at +45ºC overnight according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit). GeneChip® Fluidics Station 450 was used to wash and stain the arrays.
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G with AutoLoader was used to scan the arrays.
| | Sample_data_processing | The data were normalized and expression measures computed using the Robust Multiarray Average (RMA) method (Irizarry et. al. 2003). A linear model was fitted to the normalised intensity data with fixed constants for each replicate group using the R package 'limma' (Smyth 2004)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gavin,Charles,Preston
| | Sample_contact_laboratory | Professor Doreen A Cantrell
| | Sample_contact_department | Cell Signaling & Immunology
| | Sample_contact_institute | University of Dundee
| | Sample_contact_address | 4 Old Hawkhill
| | Sample_contact_city | Dundee
| | Sample_contact_zip/postal_code | DD1 4HN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM653nnn/GSM653199/suppl/GSM653199.CEL.gz
| | Sample_series_id | GSE26568
| | Sample_data_row_count | 45101
| | |
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GSM653200 | GPL1261 |
|
evGFP transduced CTL, biological rep. 2
|
In vitro differentiated CTL, day 4
|
tissue: In vitro cell culture
strain: C57BL/6
genotype: P14 LCMV TCR transgenic
age: 8-12 week old
|
Gene expression data from control CTL
|
| Sample_geo_accession | GSM653200
| | Sample_status | Public on May 31 2013
| | Sample_submission_date | Jan 11 2011
| | Sample_last_update_date | May 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Retroviral transduction of isolated T cells was performed at 18 hours after peptide stimulation
| | Sample_growth_protocol_ch1 | Splenocytes were activated for 48 hours with gp33-41 peptide, then washed out and cultured a further 48 hours in IL-2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Minikit (Qiagen) according to manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3’ IVT Express Kit from 250ng total RNA
| | Sample_hyb_protocol | Each sample was hybridized to GeneChip® Mouse Genome 430 2.0 Array at +45ºC overnight according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit). GeneChip® Fluidics Station 450 was used to wash and stain the arrays.
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G with AutoLoader was used to scan the arrays.
| | Sample_data_processing | The data were normalized and expression measures computed using the Robust Multiarray Average (RMA) method (Irizarry et. al. 2003). A linear model was fitted to the normalised intensity data with fixed constants for each replicate group using the R package 'limma' (Smyth 2004)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gavin,Charles,Preston
| | Sample_contact_laboratory | Professor Doreen A Cantrell
| | Sample_contact_department | Cell Signaling & Immunology
| | Sample_contact_institute | University of Dundee
| | Sample_contact_address | 4 Old Hawkhill
| | Sample_contact_city | Dundee
| | Sample_contact_zip/postal_code | DD1 4HN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM653nnn/GSM653200/suppl/GSM653200.CEL.gz
| | Sample_series_id | GSE26568
| | Sample_data_row_count | 45101
| | |
|
GSM653201 | GPL1261 |
|
evGFP transduced CTL, biological rep. 3
|
In vitro differentiated CTL, day 4
|
tissue: In vitro cell culture
strain: C57BL/6
genotype: P14 LCMV TCR transgenic
age: 8-12 week old
|
Gene expression data from control CTL
|
| Sample_geo_accession | GSM653201
| | Sample_status | Public on May 31 2013
| | Sample_submission_date | Jan 11 2011
| | Sample_last_update_date | May 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Retroviral transduction of isolated T cells was performed at 18 hours after peptide stimulation
| | Sample_growth_protocol_ch1 | Splenocytes were activated for 48 hours with gp33-41 peptide, then washed out and cultured a further 48 hours in IL-2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Minikit (Qiagen) according to manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3’ IVT Express Kit from 250ng total RNA
| | Sample_hyb_protocol | Each sample was hybridized to GeneChip® Mouse Genome 430 2.0 Array at +45ºC overnight according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit). GeneChip® Fluidics Station 450 was used to wash and stain the arrays.
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G with AutoLoader was used to scan the arrays.
| | Sample_data_processing | The data were normalized and expression measures computed using the Robust Multiarray Average (RMA) method (Irizarry et. al. 2003). A linear model was fitted to the normalised intensity data with fixed constants for each replicate group using the R package 'limma' (Smyth 2004)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gavin,Charles,Preston
| | Sample_contact_laboratory | Professor Doreen A Cantrell
| | Sample_contact_department | Cell Signaling & Immunology
| | Sample_contact_institute | University of Dundee
| | Sample_contact_address | 4 Old Hawkhill
| | Sample_contact_city | Dundee
| | Sample_contact_zip/postal_code | DD1 4HN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM653nnn/GSM653201/suppl/GSM653201.CEL.gz
| | Sample_series_id | GSE26568
| | Sample_data_row_count | 45101
| | |
|
GSM653202 | GPL1261 |
|
GFP-KLF2 transduced CTL, biological rep. 1
|
In vitro differentiated CTL, day 4
|
tissue: In vitro cell culture
strain: C57BL/6
genotype: P14 LCMV TCR transgenic
age: 8-12 week old
|
Gene expression data from CTL with enforced KLF2 expression
|
| Sample_geo_accession | GSM653202
| | Sample_status | Public on May 31 2013
| | Sample_submission_date | Jan 11 2011
| | Sample_last_update_date | May 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Retroviral transduction of isolated T cells was performed at 18 hours after peptide stimulation
| | Sample_growth_protocol_ch1 | Splenocytes were activated for 48 hours with gp33-41 peptide, then washed out and cultured a further 48 hours in IL-2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Minikit (Qiagen) according to manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3’ IVT Express Kit from 250ng total RNA
| | Sample_hyb_protocol | Each sample was hybridized to GeneChip® Mouse Genome 430 2.0 Array at +45ºC overnight according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit). GeneChip® Fluidics Station 450 was used to wash and stain the arrays.
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G with AutoLoader was used to scan the arrays.
| | Sample_data_processing | The data were normalized and expression measures computed using the Robust Multiarray Average (RMA) method (Irizarry et. al. 2003). A linear model was fitted to the normalised intensity data with fixed constants for each replicate group using the R package 'limma' (Smyth 2004)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gavin,Charles,Preston
| | Sample_contact_laboratory | Professor Doreen A Cantrell
| | Sample_contact_department | Cell Signaling & Immunology
| | Sample_contact_institute | University of Dundee
| | Sample_contact_address | 4 Old Hawkhill
| | Sample_contact_city | Dundee
| | Sample_contact_zip/postal_code | DD1 4HN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM653nnn/GSM653202/suppl/GSM653202.CEL.gz
| | Sample_series_id | GSE26568
| | Sample_data_row_count | 45101
| | |
|
GSM653203 | GPL1261 |
|
GFP-KLF2 transduced CTL, biological rep. 2
|
In vitro differentiated CTL, day 4
|
tissue: In vitro cell culture
strain: C57BL/6
genotype: P14 LCMV TCR transgenic
age: 8-12 week old
|
Gene expression data from CTL with enforced KLF2 expression
|
| Sample_geo_accession | GSM653203
| | Sample_status | Public on May 31 2013
| | Sample_submission_date | Jan 11 2011
| | Sample_last_update_date | May 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Retroviral transduction of isolated T cells was performed at 18 hours after peptide stimulation
| | Sample_growth_protocol_ch1 | Splenocytes were activated for 48 hours with gp33-41 peptide, then washed out and cultured a further 48 hours in IL-2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Minikit (Qiagen) according to manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3’ IVT Express Kit from 250ng total RNA
| | Sample_hyb_protocol | Each sample was hybridized to GeneChip® Mouse Genome 430 2.0 Array at +45ºC overnight according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit). GeneChip® Fluidics Station 450 was used to wash and stain the arrays.
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G with AutoLoader was used to scan the arrays.
| | Sample_data_processing | The data were normalized and expression measures computed using the Robust Multiarray Average (RMA) method (Irizarry et. al. 2003). A linear model was fitted to the normalised intensity data with fixed constants for each replicate group using the R package 'limma' (Smyth 2004)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gavin,Charles,Preston
| | Sample_contact_laboratory | Professor Doreen A Cantrell
| | Sample_contact_department | Cell Signaling & Immunology
| | Sample_contact_institute | University of Dundee
| | Sample_contact_address | 4 Old Hawkhill
| | Sample_contact_city | Dundee
| | Sample_contact_zip/postal_code | DD1 4HN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM653nnn/GSM653203/suppl/GSM653203.CEL.gz
| | Sample_series_id | GSE26568
| | Sample_data_row_count | 45101
| | |
|
GSM653204 | GPL1261 |
|
GFP-KLF2 transduced CTL, biological rep. 3
|
In vitro differentiated CTL, day 4
|
tissue: In vitro cell culture
strain: C57BL/6
genotype: P14 LCMV TCR transgenic
age: 8-12 week old
|
Gene expression data from CTL with enforced KLF2 expression
|
| Sample_geo_accession | GSM653204
| | Sample_status | Public on May 31 2013
| | Sample_submission_date | Jan 11 2011
| | Sample_last_update_date | May 31 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Retroviral transduction of isolated T cells was performed at 18 hours after peptide stimulation
| | Sample_growth_protocol_ch1 | Splenocytes were activated for 48 hours with gp33-41 peptide, then washed out and cultured a further 48 hours in IL-2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Minikit (Qiagen) according to manufacturers instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix GeneChip 3’ IVT Express Kit from 250ng total RNA
| | Sample_hyb_protocol | Each sample was hybridized to GeneChip® Mouse Genome 430 2.0 Array at +45ºC overnight according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit). GeneChip® Fluidics Station 450 was used to wash and stain the arrays.
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G with AutoLoader was used to scan the arrays.
| | Sample_data_processing | The data were normalized and expression measures computed using the Robust Multiarray Average (RMA) method (Irizarry et. al. 2003). A linear model was fitted to the normalised intensity data with fixed constants for each replicate group using the R package 'limma' (Smyth 2004)
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gavin,Charles,Preston
| | Sample_contact_laboratory | Professor Doreen A Cantrell
| | Sample_contact_department | Cell Signaling & Immunology
| | Sample_contact_institute | University of Dundee
| | Sample_contact_address | 4 Old Hawkhill
| | Sample_contact_city | Dundee
| | Sample_contact_zip/postal_code | DD1 4HN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM653nnn/GSM653204/suppl/GSM653204.CEL.gz
| | Sample_series_id | GSE26568
| | Sample_data_row_count | 45101
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