Search results for the GEO ID: GSE26594  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM654536 | GPL570 |
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FS087 cell line, control, biological rep1
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Human primary lung fibroblast from an IPF patient maintained in standard culture
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treatment group: Unstimulated
cell type: Human primary lung fibroblast
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FS087_control
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| Sample_geo_accession | GSM654536
| | Sample_status | Public on Jan 13 2011
| | Sample_submission_date | Jan 12 2011
| | Sample_last_update_date | Jan 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Each cell line was divided into two groups and half of each was treated with TNF-α (10 ng/ml) and IFN-γ (50 U/ml) added directly to the growing media and both groups allowed to incubate for 36 hr at standard conditions.
| | Sample_growth_protocol_ch1 | All cell lines used in the study were maintained in modified Eagle’s Minimum Essential Medium, supplemented 100 U/ml penicillin, 100 µg/ml streptomycin and 10% (v/v) heat inactivated fetal bovine serum, under a 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 5 micrograms of total RNA was processed to produce biotinylated cRNA targets using the older (ENZO) Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix procedures
| | Sample_scan_protocol | standard Affymetrix procedures, using the Affy GCS3000
| | Sample_data_processing | GCRMA normalization with Partek Genomics Suite 6.5.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM654nnn/GSM654536/suppl/GSM654536.CEL.gz
| | Sample_series_id | GSE26594
| | Sample_data_row_count | 54675
| | |
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GSM654537 | GPL570 |
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FS087 cell line, stimulated, biological rep1
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Human primary lung fibroblast from an IPF patient treated for 36 hr with TNF-α (10 ng/ml) and IFN-γ (50 U/ml).
|
treatment group: TNF-α and IFN-γ stimulated
cell type: Human primary lung fibroblast
|
FS087_stimulated
|
| Sample_geo_accession | GSM654537
| | Sample_status | Public on Jan 13 2011
| | Sample_submission_date | Jan 12 2011
| | Sample_last_update_date | Jan 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Each cell line was divided into two groups and half of each was treated with TNF-α (10 ng/ml) and IFN-γ (50 U/ml) added directly to the growing media and both groups allowed to incubate for 36 hr at standard conditions.
| | Sample_growth_protocol_ch1 | All cell lines used in the study were maintained in modified Eagle’s Minimum Essential Medium, supplemented 100 U/ml penicillin, 100 µg/ml streptomycin and 10% (v/v) heat inactivated fetal bovine serum, under a 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 5 micrograms of total RNA was processed to produce biotinylated cRNA targets using the older (ENZO) Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix procedures
| | Sample_scan_protocol | standard Affymetrix procedures, using the Affy GCS3000
| | Sample_data_processing | GCRMA normalization with Partek Genomics Suite 6.5.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM654nnn/GSM654537/suppl/GSM654537.CEL.gz
| | Sample_series_id | GSE26594
| | Sample_data_row_count | 54675
| | |
|
GSM654538 | GPL570 |
|
MRC5 cell line, control, biological rep2
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Human fetal lung fibroblast cell line maintained in standard culture
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treatment group: Unstimulated
cell type: Human fetal lung fibroblast cell line
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MRC5_control
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| Sample_geo_accession | GSM654538
| | Sample_status | Public on Jan 13 2011
| | Sample_submission_date | Jan 12 2011
| | Sample_last_update_date | Jan 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Each cell line was divided into two groups and half of each was treated with TNF-α (10 ng/ml) and IFN-γ (50 U/ml) added directly to the growing media and both groups allowed to incubate for 36 hr at standard conditions.
| | Sample_growth_protocol_ch1 | All cell lines used in the study were maintained in modified Eagle’s Minimum Essential Medium, supplemented 100 U/ml penicillin, 100 µg/ml streptomycin and 10% (v/v) heat inactivated fetal bovine serum, under a 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 5 micrograms of total RNA was processed to produce biotinylated cRNA targets using the older (ENZO) Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix procedures
| | Sample_scan_protocol | standard Affymetrix procedures, using the Affy GCS3000
| | Sample_data_processing | GCRMA normalization with Partek Genomics Suite 6.5.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM654nnn/GSM654538/suppl/GSM654538.CEL.gz
| | Sample_series_id | GSE26594
| | Sample_data_row_count | 54675
| | |
|
GSM654539 | GPL570 |
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MRC5 cell line, stimulated, biological rep2
|
Human fetal lung fibroblast cell line treated for 36 hr with TNF-α (10 ng/ml) and IFN-γ (50 U/ml).
|
treatment group: TNF-α and IFN-γ stimulated
cell type: Human fetal lung fibroblast cell line
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MRC5_stimulated
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| Sample_geo_accession | GSM654539
| | Sample_status | Public on Jan 13 2011
| | Sample_submission_date | Jan 12 2011
| | Sample_last_update_date | Jan 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Each cell line was divided into two groups and half of each was treated with TNF-α (10 ng/ml) and IFN-γ (50 U/ml) added directly to the growing media and both groups allowed to incubate for 36 hr at standard conditions.
| | Sample_growth_protocol_ch1 | All cell lines used in the study were maintained in modified Eagle’s Minimum Essential Medium, supplemented 100 U/ml penicillin, 100 µg/ml streptomycin and 10% (v/v) heat inactivated fetal bovine serum, under a 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 5 micrograms of total RNA was processed to produce biotinylated cRNA targets using the older (ENZO) Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix procedures
| | Sample_scan_protocol | standard Affymetrix procedures, using the Affy GCS3000
| | Sample_data_processing | GCRMA normalization with Partek Genomics Suite 6.5.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM654nnn/GSM654539/suppl/GSM654539.CEL.gz
| | Sample_series_id | GSE26594
| | Sample_data_row_count | 54675
| | |
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GSM654540 | GPL570 |
|
N78 cell line, control, biological rep3
|
Human primary lung fibroblast from a control subject maintained in standard culture
|
treatment group: Unstimulated
cell type: Human primary lung fibroblast
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N78_control
|
| Sample_geo_accession | GSM654540
| | Sample_status | Public on Jan 13 2011
| | Sample_submission_date | Jan 12 2011
| | Sample_last_update_date | Jan 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Each cell line was divided into two groups and half of each was treated with TNF-α (10 ng/ml) and IFN-γ (50 U/ml) added directly to the growing media and both groups allowed to incubate for 36 hr at standard conditions.
| | Sample_growth_protocol_ch1 | All cell lines used in the study were maintained in modified Eagle’s Minimum Essential Medium, supplemented 100 U/ml penicillin, 100 µg/ml streptomycin and 10% (v/v) heat inactivated fetal bovine serum, under a 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 5 micrograms of total RNA was processed to produce biotinylated cRNA targets using the older (ENZO) Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix procedures
| | Sample_scan_protocol | standard Affymetrix procedures, using the Affy GCS3000
| | Sample_data_processing | GCRMA normalization with Partek Genomics Suite 6.5.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM654nnn/GSM654540/suppl/GSM654540.CEL.gz
| | Sample_series_id | GSE26594
| | Sample_data_row_count | 54675
| | |
|
GSM654541 | GPL570 |
|
N78 cell line, stimulated, biological rep3
|
Human primary lung fibroblast from a control subject treated for 36 hr with TNF-α (10 ng/ml) and IFN-γ (50 U/ml).
|
treatment group: TNF-α and IFN-γ stimulated
cell type: Human primary lung fibroblast
|
N78_stimulated
|
| Sample_geo_accession | GSM654541
| | Sample_status | Public on Jan 13 2011
| | Sample_submission_date | Jan 12 2011
| | Sample_last_update_date | Jan 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Each cell line was divided into two groups and half of each was treated with TNF-α (10 ng/ml) and IFN-γ (50 U/ml) added directly to the growing media and both groups allowed to incubate for 36 hr at standard conditions.
| | Sample_growth_protocol_ch1 | All cell lines used in the study were maintained in modified Eagle’s Minimum Essential Medium, supplemented 100 U/ml penicillin, 100 µg/ml streptomycin and 10% (v/v) heat inactivated fetal bovine serum, under a 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 5 micrograms of total RNA was processed to produce biotinylated cRNA targets using the older (ENZO) Affymetrix protocol
| | Sample_hyb_protocol | standard Affymetrix procedures
| | Sample_scan_protocol | standard Affymetrix procedures, using the Affy GCS3000
| | Sample_data_processing | GCRMA normalization with Partek Genomics Suite 6.5.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM654nnn/GSM654541/suppl/GSM654541.CEL.gz
| | Sample_series_id | GSE26594
| | Sample_data_row_count | 54675
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