Search results for the GEO ID: GSE26656  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM656286 | GPL570 |
|
A375 control-1
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: wild type
treatment: control
|
A375 Co_1
|
| Sample_geo_accession | GSM656286
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656286/suppl/GSM656286.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656287 | GPL570 |
|
A375 control-2
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: wild type
treatment: control
|
A375 Co_2
|
| Sample_geo_accession | GSM656287
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656287/suppl/GSM656287.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656288 | GPL570 |
|
A375 control-3
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: wild type
treatment: control
|
A375 Co_3
|
| Sample_geo_accession | GSM656288
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656288/suppl/GSM656288.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656289 | GPL570 |
|
A375 control-4
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: wild type
treatment: control
|
A375 Co_4
|
| Sample_geo_accession | GSM656289
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656289/suppl/GSM656289.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656290 | GPL570 |
|
A375 Wnt1-1
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: Wnt-1 overexpressing
treatment: control
|
A375 Wnt1_1
|
| Sample_geo_accession | GSM656290
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656290/suppl/GSM656290.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656291 | GPL570 |
|
A375 Wnt1-2
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: Wnt-1 overexpressing
treatment: control
|
A375 Wnt1_2
|
| Sample_geo_accession | GSM656291
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656291/suppl/GSM656291.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656292 | GPL570 |
|
A375 Wnt1-3
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: Wnt-1 overexpressing
treatment: control
|
A375 Wnt1_3
|
| Sample_geo_accession | GSM656292
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656292/suppl/GSM656292.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656293 | GPL570 |
|
A375 control+CsA -1
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: wild type
treatment: animals were fed with CsA (50mg/kg/day)
|
A375Co-CsA_1
|
| Sample_geo_accession | GSM656293
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656293/suppl/GSM656293.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656294 | GPL570 |
|
A375 control+CsA -2
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: wild type
treatment: animals were fed with CsA (50mg/kg/day)
|
A375Co-CsA_2
|
| Sample_geo_accession | GSM656294
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656294/suppl/GSM656294.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656295 | GPL570 |
|
A375 control+CsA -3
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: wild type
treatment: animals were fed with CsA (50mg/kg/day)
|
A375Co-CsA_3
|
| Sample_geo_accession | GSM656295
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656295/suppl/GSM656295.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656296 | GPL570 |
|
A375 control+CsA -4
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: wild type
treatment: animals were fed with CsA (50mg/kg/day)
|
A375Co-CsA_4
|
| Sample_geo_accession | GSM656296
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656296/suppl/GSM656296.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656297 | GPL570 |
|
A375 Wnt1+CsA-1
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: Wnt-1 overexpressing
treatment: animals were fed with CsA (50mg/kg/day)
|
A375 Wnt1-CsA_1
|
| Sample_geo_accession | GSM656297
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656297/suppl/GSM656297.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656298 | GPL570 |
|
A375 Wnt1+CsA-2
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: Wnt-1 overexpressing
treatment: animals were fed with CsA (50mg/kg/day)
|
A375 Wnt1-CsA_2
|
| Sample_geo_accession | GSM656298
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656298/suppl/GSM656298.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656299 | GPL570 |
|
A375 Wnt1+CsA-3
|
A375 melanoma cell-line
|
cell line: A375 melanoma cell-line
genotype/variation: Wnt-1 overexpressing
treatment: animals were fed with CsA (50mg/kg/day)
|
A375 Wnt1-CsA_3
|
| Sample_geo_accession | GSM656299
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656299/suppl/GSM656299.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656300 | GPL570 |
|
M24met_control_1
|
M24met melanoma cell-line
|
cell line: M24met melanoma cell-line
genotype/variation: wild type
treatment: control
|
M24 Co_1
|
| Sample_geo_accession | GSM656300
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656300/suppl/GSM656300.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656301 | GPL570 |
|
M24met_control_2
|
M24met melanoma cell-line
|
cell line: M24met melanoma cell-line
genotype/variation: wild type
treatment: control
|
M24 Co_2
|
| Sample_geo_accession | GSM656301
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656301/suppl/GSM656301.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656302 | GPL570 |
|
M24met_control_3
|
M24met melanoma cell-line
|
cell line: M24met melanoma cell-line
genotype/variation: wild type
treatment: control
|
M24 Co_3
|
| Sample_geo_accession | GSM656302
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656302/suppl/GSM656302.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656303 | GPL570 |
|
M24met_control_4
|
M24met melanoma cell-line
|
cell line: M24met melanoma cell-line
genotype/variation: wild type
treatment: control
|
M24 Co_4
|
| Sample_geo_accession | GSM656303
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656303/suppl/GSM656303.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656304 | GPL570 |
|
M24met_control_5
|
M24met melanoma cell-line
|
cell line: M24met melanoma cell-line
genotype/variation: wild type
treatment: control
|
M24 Co_5
|
| Sample_geo_accession | GSM656304
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656304/suppl/GSM656304.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656305 | GPL570 |
|
M24met_Wnt1_1
|
M24met melanoma cell-line
|
cell line: M24met melanoma cell-line
genotype/variation: Wnt-1 overexpressing
treatment: control
|
M24 Wnt1_1
|
| Sample_geo_accession | GSM656305
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656305/suppl/GSM656305.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656306 | GPL570 |
|
M24met_Wnt1_2
|
M24met melanoma cell-line
|
cell line: M24met melanoma cell-line
genotype/variation: Wnt-1 overexpressing
treatment: control
|
M24 Wnt1_2
|
| Sample_geo_accession | GSM656306
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656306/suppl/GSM656306.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656307 | GPL570 |
|
M24met_Wnt1_3
|
M24met melanoma cell-line
|
cell line: M24met melanoma cell-line
genotype/variation: Wnt-1 overexpressing
treatment: control
|
M24 Wnt1_3
|
| Sample_geo_accession | GSM656307
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656307/suppl/GSM656307.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
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GSM656308 | GPL570 |
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M24met_Wnt1_4
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M24met melanoma cell-line
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cell line: M24met melanoma cell-line
genotype/variation: Wnt-1 overexpressing
treatment: control
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M24 Wnt1_4
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| Sample_geo_accession | GSM656308
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656308/suppl/GSM656308.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
|
GSM656309 | GPL570 |
|
M24met_Wnt1_5
|
M24met melanoma cell-line
|
cell line: M24met melanoma cell-line
genotype/variation: Wnt-1 overexpressing
treatment: control
|
M24 Wnt1_5
|
| Sample_geo_accession | GSM656309
| | Sample_status | Public on Jun 05 2012
| | Sample_submission_date | Jan 17 2011
| | Sample_last_update_date | Jun 05 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Where indicated, animals were fed with CsA (50mg/kg/day) via a gauge
| | Sample_growth_protocol_ch1 | Control and Wnt-1 overexpressing human melanoma cell-lines (A375 and M24met) were injected into the right flank of SCID mice. Primary tumors were removed after a mean tumor volume of 400m3 and RNA was prepared. For Affymetrix chips, 5µg of RNA were reverse transcribed into second strand cDNA. Preparation of cRNA, hybridization to Human Genome U133 Plus 2.0 (Affymetrix) were carried out according to the manufacturer’s protocols (www.affymetrix.com).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | SCID mouse-derived samples were homogenized with RLT lysis buffer using a mixer mill MM200. Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | biotin (GeneChip Expression 3´Amplification One-Cycle Target Labeling and Control Reagents (30Rxn)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix Scanner 3000 7G
| | Sample_data_processing | RMA normalization using R 2.9 and BioC 2.4
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stefanie,,Tauber
| | Sample_contact_email | stefanie.tauber@univie.ac.at
| | Sample_contact_department | CIBIV
| | Sample_contact_institute | MFPL
| | Sample_contact_address | Dr. Bohr-Gasse 9
| | Sample_contact_city | Vienna
| | Sample_contact_zip/postal_code | 1030
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM656nnn/GSM656309/suppl/GSM656309.CEL.gz
| | Sample_series_id | GSE26656
| | Sample_data_row_count | 54675
| | |
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