Search results for the GEO ID: GSE26704 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM657239 | GPL570 |
|
H460v-control
|
H460v-control
|
cell line: non small cell lung cancer cell line H460
genotype/variation: wild type
agent: untreated (control)
|
07SE213
|
Sample_geo_accession | GSM657239
| Sample_status | Public on Jan 01 2012
| Sample_submission_date | Jan 19 2011
| Sample_last_update_date | Jan 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The DUSP1 pSuperRetro-derived vectors were constructed as described (Chattoppadhyay et al., 2006). Clones expressing siRNA for DUSP1 were selected for their ability to grow in the presence of puromycin and kept for selection. Stable transfection was confirmed by qRT-PCR and Western Blot.Cisplatin (CCDP) was purchased by Farma Ferrer (Barcelona, Spain). Cells were treated with 10 µg/mL CDDP for 1, 3 and 6 hours.
| Sample_growth_protocol_ch1 | The human non small lung cancer cell line H460 used in this study was from ATCC (American Type Culture Collection). The cell line was maintained in Roswell Park Memorial Institute (RPMI) (Gibco, Invitrogen) supplemented with 10% bovine serum and transfected by Lipofectamine Plus Reagent from Life Technologies as directed by the manufacturer.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany). The RNA quality was confirmed by the ratio of 28S and 18S rRNA after agarose gel electrophoresis.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The cDNA was prepared from 2 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Eva,,Bandres
| Sample_contact_email | ebandres@unav.es
| Sample_contact_laboratory | Pharmacogenomics lab
| Sample_contact_department | Cancer Research Program
| Sample_contact_institute | Center for Applied Medical Research (CIMA), University of Navarra
| Sample_contact_address | Avda Pio XII, 55
| Sample_contact_city | Pamplona
| Sample_contact_zip/postal_code | 31008
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM657nnn/GSM657239/suppl/GSM657239.CEL.gz
| Sample_series_id | GSE26704
| Sample_data_row_count | 54675
| |
|
GSM657240 | GPL570 |
|
H460cri-control
|
H460cri-control
|
cell line: non small cell lung cancer cell line H460
genotype/variation: DUSP1 knockdown (siRNA-mediated)
agent: untreated (control)
|
07SE214
|
Sample_geo_accession | GSM657240
| Sample_status | Public on Jan 01 2012
| Sample_submission_date | Jan 19 2011
| Sample_last_update_date | Jan 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The DUSP1 pSuperRetro-derived vectors were constructed as described (Chattoppadhyay et al., 2006). Clones expressing siRNA for DUSP1 were selected for their ability to grow in the presence of puromycin and kept for selection. Stable transfection was confirmed by qRT-PCR and Western Blot.Cisplatin (CCDP) was purchased by Farma Ferrer (Barcelona, Spain). Cells were treated with 10 µg/mL CDDP for 1, 3 and 6 hours.
| Sample_growth_protocol_ch1 | The human non small lung cancer cell line H460 used in this study was from ATCC (American Type Culture Collection). The cell line was maintained in Roswell Park Memorial Institute (RPMI) (Gibco, Invitrogen) supplemented with 10% bovine serum and transfected by Lipofectamine Plus Reagent from Life Technologies as directed by the manufacturer.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany). The RNA quality was confirmed by the ratio of 28S and 18S rRNA after agarose gel electrophoresis.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The cDNA was prepared from 2 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Eva,,Bandres
| Sample_contact_email | ebandres@unav.es
| Sample_contact_laboratory | Pharmacogenomics lab
| Sample_contact_department | Cancer Research Program
| Sample_contact_institute | Center for Applied Medical Research (CIMA), University of Navarra
| Sample_contact_address | Avda Pio XII, 55
| Sample_contact_city | Pamplona
| Sample_contact_zip/postal_code | 31008
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM657nnn/GSM657240/suppl/GSM657240.CEL.gz
| Sample_series_id | GSE26704
| Sample_data_row_count | 54675
| |
|
GSM657241 | GPL570 |
|
H460v-CCDP 1hour
|
H460v-CCDP 1hour
|
cell line: non small cell lung cancer cell line H460
genotype/variation: wild type
agent: CCDP
time: 1 hour
|
07SE215
|
Sample_geo_accession | GSM657241
| Sample_status | Public on Jan 01 2012
| Sample_submission_date | Jan 19 2011
| Sample_last_update_date | Jan 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The DUSP1 pSuperRetro-derived vectors were constructed as described (Chattoppadhyay et al., 2006). Clones expressing siRNA for DUSP1 were selected for their ability to grow in the presence of puromycin and kept for selection. Stable transfection was confirmed by qRT-PCR and Western Blot.Cisplatin (CCDP) was purchased by Farma Ferrer (Barcelona, Spain). Cells were treated with 10 µg/mL CDDP for 1, 3 and 6 hours.
| Sample_growth_protocol_ch1 | The human non small lung cancer cell line H460 used in this study was from ATCC (American Type Culture Collection). The cell line was maintained in Roswell Park Memorial Institute (RPMI) (Gibco, Invitrogen) supplemented with 10% bovine serum and transfected by Lipofectamine Plus Reagent from Life Technologies as directed by the manufacturer.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany). The RNA quality was confirmed by the ratio of 28S and 18S rRNA after agarose gel electrophoresis.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The cDNA was prepared from 2 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Eva,,Bandres
| Sample_contact_email | ebandres@unav.es
| Sample_contact_laboratory | Pharmacogenomics lab
| Sample_contact_department | Cancer Research Program
| Sample_contact_institute | Center for Applied Medical Research (CIMA), University of Navarra
| Sample_contact_address | Avda Pio XII, 55
| Sample_contact_city | Pamplona
| Sample_contact_zip/postal_code | 31008
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM657nnn/GSM657241/suppl/GSM657241.CEL.gz
| Sample_series_id | GSE26704
| Sample_data_row_count | 54675
| |
|
GSM657242 | GPL570 |
|
H460cri-CCDP 1hour
|
H460cri-CCDP 1hour
|
cell line: non small cell lung cancer cell line H460
genotype/variation: DUSP1 knockdown (siRNA-mediated)
agent: CCDP
time: 1 hour
|
07SE216
|
Sample_geo_accession | GSM657242
| Sample_status | Public on Jan 01 2012
| Sample_submission_date | Jan 19 2011
| Sample_last_update_date | Jan 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The DUSP1 pSuperRetro-derived vectors were constructed as described (Chattoppadhyay et al., 2006). Clones expressing siRNA for DUSP1 were selected for their ability to grow in the presence of puromycin and kept for selection. Stable transfection was confirmed by qRT-PCR and Western Blot.Cisplatin (CCDP) was purchased by Farma Ferrer (Barcelona, Spain). Cells were treated with 10 µg/mL CDDP for 1, 3 and 6 hours.
| Sample_growth_protocol_ch1 | The human non small lung cancer cell line H460 used in this study was from ATCC (American Type Culture Collection). The cell line was maintained in Roswell Park Memorial Institute (RPMI) (Gibco, Invitrogen) supplemented with 10% bovine serum and transfected by Lipofectamine Plus Reagent from Life Technologies as directed by the manufacturer.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany). The RNA quality was confirmed by the ratio of 28S and 18S rRNA after agarose gel electrophoresis.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The cDNA was prepared from 2 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Eva,,Bandres
| Sample_contact_email | ebandres@unav.es
| Sample_contact_laboratory | Pharmacogenomics lab
| Sample_contact_department | Cancer Research Program
| Sample_contact_institute | Center for Applied Medical Research (CIMA), University of Navarra
| Sample_contact_address | Avda Pio XII, 55
| Sample_contact_city | Pamplona
| Sample_contact_zip/postal_code | 31008
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM657nnn/GSM657242/suppl/GSM657242.CEL.gz
| Sample_series_id | GSE26704
| Sample_data_row_count | 54675
| |
|
GSM657243 | GPL570 |
|
H460v-CCDP 3 hour
|
H460v-CCDP 3 hour
|
cell line: non small cell lung cancer cell line H460
genotype/variation: wild type
agent: CCDP
time: 3 hour
|
07SE217
|
Sample_geo_accession | GSM657243
| Sample_status | Public on Jan 01 2012
| Sample_submission_date | Jan 19 2011
| Sample_last_update_date | Jan 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The DUSP1 pSuperRetro-derived vectors were constructed as described (Chattoppadhyay et al., 2006). Clones expressing siRNA for DUSP1 were selected for their ability to grow in the presence of puromycin and kept for selection. Stable transfection was confirmed by qRT-PCR and Western Blot.Cisplatin (CCDP) was purchased by Farma Ferrer (Barcelona, Spain). Cells were treated with 10 µg/mL CDDP for 1, 3 and 6 hours.
| Sample_growth_protocol_ch1 | The human non small lung cancer cell line H460 used in this study was from ATCC (American Type Culture Collection). The cell line was maintained in Roswell Park Memorial Institute (RPMI) (Gibco, Invitrogen) supplemented with 10% bovine serum and transfected by Lipofectamine Plus Reagent from Life Technologies as directed by the manufacturer.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany). The RNA quality was confirmed by the ratio of 28S and 18S rRNA after agarose gel electrophoresis.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The cDNA was prepared from 2 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Eva,,Bandres
| Sample_contact_email | ebandres@unav.es
| Sample_contact_laboratory | Pharmacogenomics lab
| Sample_contact_department | Cancer Research Program
| Sample_contact_institute | Center for Applied Medical Research (CIMA), University of Navarra
| Sample_contact_address | Avda Pio XII, 55
| Sample_contact_city | Pamplona
| Sample_contact_zip/postal_code | 31008
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM657nnn/GSM657243/suppl/GSM657243.CEL.gz
| Sample_series_id | GSE26704
| Sample_data_row_count | 54675
| |
|
GSM657244 | GPL570 |
|
H460cri-CCDP 3 hour
|
H460cri-CCDP 3 hour
|
cell line: non small cell lung cancer cell line H460
genotype/variation: DUSP1 knockdown (siRNA-mediated)
agent: CCDP
time: 3 hour
|
07SE218
|
Sample_geo_accession | GSM657244
| Sample_status | Public on Jan 01 2012
| Sample_submission_date | Jan 19 2011
| Sample_last_update_date | Jan 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The DUSP1 pSuperRetro-derived vectors were constructed as described (Chattoppadhyay et al., 2006). Clones expressing siRNA for DUSP1 were selected for their ability to grow in the presence of puromycin and kept for selection. Stable transfection was confirmed by qRT-PCR and Western Blot.Cisplatin (CCDP) was purchased by Farma Ferrer (Barcelona, Spain). Cells were treated with 10 µg/mL CDDP for 1, 3 and 6 hours.
| Sample_growth_protocol_ch1 | The human non small lung cancer cell line H460 used in this study was from ATCC (American Type Culture Collection). The cell line was maintained in Roswell Park Memorial Institute (RPMI) (Gibco, Invitrogen) supplemented with 10% bovine serum and transfected by Lipofectamine Plus Reagent from Life Technologies as directed by the manufacturer.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany). The RNA quality was confirmed by the ratio of 28S and 18S rRNA after agarose gel electrophoresis.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The cDNA was prepared from 2 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Eva,,Bandres
| Sample_contact_email | ebandres@unav.es
| Sample_contact_laboratory | Pharmacogenomics lab
| Sample_contact_department | Cancer Research Program
| Sample_contact_institute | Center for Applied Medical Research (CIMA), University of Navarra
| Sample_contact_address | Avda Pio XII, 55
| Sample_contact_city | Pamplona
| Sample_contact_zip/postal_code | 31008
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM657nnn/GSM657244/suppl/GSM657244.CEL.gz
| Sample_series_id | GSE26704
| Sample_data_row_count | 54675
| |
|
GSM657245 | GPL570 |
|
H460v-CCDP 6hour
|
H460v-CCDP 6hour
|
cell line: non small cell lung cancer cell line H460
genotype/variation: wild type
agent: CCDP
time: 6 hour
|
07SE219
|
Sample_geo_accession | GSM657245
| Sample_status | Public on Jan 01 2012
| Sample_submission_date | Jan 19 2011
| Sample_last_update_date | Jan 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The DUSP1 pSuperRetro-derived vectors were constructed as described (Chattoppadhyay et al., 2006). Clones expressing siRNA for DUSP1 were selected for their ability to grow in the presence of puromycin and kept for selection. Stable transfection was confirmed by qRT-PCR and Western Blot.Cisplatin (CCDP) was purchased by Farma Ferrer (Barcelona, Spain). Cells were treated with 10 µg/mL CDDP for 1, 3 and 6 hours.
| Sample_growth_protocol_ch1 | The human non small lung cancer cell line H460 used in this study was from ATCC (American Type Culture Collection). The cell line was maintained in Roswell Park Memorial Institute (RPMI) (Gibco, Invitrogen) supplemented with 10% bovine serum and transfected by Lipofectamine Plus Reagent from Life Technologies as directed by the manufacturer.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany). The RNA quality was confirmed by the ratio of 28S and 18S rRNA after agarose gel electrophoresis.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The cDNA was prepared from 2 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Eva,,Bandres
| Sample_contact_email | ebandres@unav.es
| Sample_contact_laboratory | Pharmacogenomics lab
| Sample_contact_department | Cancer Research Program
| Sample_contact_institute | Center for Applied Medical Research (CIMA), University of Navarra
| Sample_contact_address | Avda Pio XII, 55
| Sample_contact_city | Pamplona
| Sample_contact_zip/postal_code | 31008
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM657nnn/GSM657245/suppl/GSM657245.CEL.gz
| Sample_series_id | GSE26704
| Sample_data_row_count | 54675
| |
|
GSM657246 | GPL570 |
|
H460cri-CCDP 6hour
|
H460cri-CCDP 6hour
|
cell line: non small cell lung cancer cell line H460
genotype/variation: DUSP1 knockdown (siRNA-mediated)
agent: CCDP
time: 6 hour
|
07SE220
|
Sample_geo_accession | GSM657246
| Sample_status | Public on Jan 01 2012
| Sample_submission_date | Jan 19 2011
| Sample_last_update_date | Jan 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The DUSP1 pSuperRetro-derived vectors were constructed as described (Chattoppadhyay et al., 2006). Clones expressing siRNA for DUSP1 were selected for their ability to grow in the presence of puromycin and kept for selection. Stable transfection was confirmed by qRT-PCR and Western Blot.Cisplatin (CCDP) was purchased by Farma Ferrer (Barcelona, Spain). Cells were treated with 10 µg/mL CDDP for 1, 3 and 6 hours.
| Sample_growth_protocol_ch1 | The human non small lung cancer cell line H460 used in this study was from ATCC (American Type Culture Collection). The cell line was maintained in Roswell Park Memorial Institute (RPMI) (Gibco, Invitrogen) supplemented with 10% bovine serum and transfected by Lipofectamine Plus Reagent from Life Technologies as directed by the manufacturer.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany). The RNA quality was confirmed by the ratio of 28S and 18S rRNA after agarose gel electrophoresis.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The cDNA was prepared from 2 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
| Sample_hyb_protocol | Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133A 2.0 chip according to the protocols provided by the manufacturer.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Eva,,Bandres
| Sample_contact_email | ebandres@unav.es
| Sample_contact_laboratory | Pharmacogenomics lab
| Sample_contact_department | Cancer Research Program
| Sample_contact_institute | Center for Applied Medical Research (CIMA), University of Navarra
| Sample_contact_address | Avda Pio XII, 55
| Sample_contact_city | Pamplona
| Sample_contact_zip/postal_code | 31008
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM657nnn/GSM657246/suppl/GSM657246.CEL.gz
| Sample_series_id | GSE26704
| Sample_data_row_count | 54675
| |
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