Result

Search results for the GEO ID: GSE26730

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM658097

GPL1355

granulosa cell.6h Ad-GFP

ovarian granulosa cells collected from ovaries 48 h after stimulation with pregnant mare's serum gonadotropin

strain: Sprague-Dawley gender: Female age: Immature 24-25 days old

Sample_geo_accession	GSM658097
Sample_status	Public on Jan 20 2011
Sample_submission_date	Jan 19 2011
Sample_last_update_date	Jan 20 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The granulosa cells were exposed to Ad- NFIL3 or Ad-GFP at a multiplicity of infection (MOI) of 50 pfu/cell. Two hours later, Medium was replaced with fresh Opti-MEM medium. At 24 h after adenovirus exposure, granulosa cells were treated with FSK (10uM) + PMA (20 nM) for 6 h before collection for RNA isolation using a RNeasy kit according to the manufacturer’s instructions (Qiagen Inc., Valencia, CA).
Sample_growth_protocol_ch1	To isolate granulosa cells, ovaries were collected from rats 48 h after PMSG administration and processed as described previously. Briefly, granulosa cells were isolated by follicular puncture, pooled, filtered, pelleted by centrifugation at 200 x g for 5 min, and resuspended in Opti-MEM medium supplemented with 0.05 mg/ml of gentamycin and 1x ITS (insulin, transferin, and selenium). The cells were cultured in the absence or presence of various reagents discussed in detail below for different time points at 37oC in a humidified atmosphere of 5% CO2. Forskolin (FSK) and phorbol 12-myristate 13-acetate (PMA) were added to mimic hCG action.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Standard Affymetrix
Sample_label_ch1	Standard Affymetrix
Sample_label_protocol_ch1	Standard Affymetrix
Sample_hyb_protocol	Standard Affymetrix
Sample_scan_protocol	Standard Affymetrix
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Eric,M,Blalock
Sample_contact_email	emblal@uky.edu
Sample_contact_phone	859-323-8033
Sample_contact_laboratory	Blalock
Sample_contact_department	Molecular and Biomedical Pharmacology
Sample_contact_institute	University of Kentucky
Sample_contact_address	800 Rose St.
Sample_contact_city	Lexington
Sample_contact_state	KY
Sample_contact_zip/postal_code	40475
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658097/suppl/GSM658097.CEL.gz
Sample_series_id	GSE26730
Sample_data_row_count	31099

GSM658098

GPL1355

granulosa cell.6h Ad-GFP +FSK/PMA

ovarian granulosa cells collected from ovaries 48 h after stimulation with pregnant mare's serum gonadotropin

strain: Sprague-Dawley gender: Female age: Immature 24-25 days old

Sample_geo_accession	GSM658098
Sample_status	Public on Jan 20 2011
Sample_submission_date	Jan 19 2011
Sample_last_update_date	Jan 20 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The granulosa cells were exposed to Ad- NFIL3 or Ad-GFP at a multiplicity of infection (MOI) of 50 pfu/cell. Two hours later, Medium was replaced with fresh Opti-MEM medium. At 24 h after adenovirus exposure, granulosa cells were treated with FSK (10uM) + PMA (20 nM) for 6 h before collection for RNA isolation using a RNeasy kit according to the manufacturer’s instructions (Qiagen Inc., Valencia, CA).
Sample_growth_protocol_ch1	To isolate granulosa cells, ovaries were collected from rats 48 h after PMSG administration and processed as described previously. Briefly, granulosa cells were isolated by follicular puncture, pooled, filtered, pelleted by centrifugation at 200 x g for 5 min, and resuspended in Opti-MEM medium supplemented with 0.05 mg/ml of gentamycin and 1x ITS (insulin, transferin, and selenium). The cells were cultured in the absence or presence of various reagents discussed in detail below for different time points at 37oC in a humidified atmosphere of 5% CO2. Forskolin (FSK) and phorbol 12-myristate 13-acetate (PMA) were added to mimic hCG action.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Standard Affymetrix
Sample_label_ch1	Standard Affymetrix
Sample_label_protocol_ch1	Standard Affymetrix
Sample_hyb_protocol	Standard Affymetrix
Sample_scan_protocol	Standard Affymetrix
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Eric,M,Blalock
Sample_contact_email	emblal@uky.edu
Sample_contact_phone	859-323-8033
Sample_contact_laboratory	Blalock
Sample_contact_department	Molecular and Biomedical Pharmacology
Sample_contact_institute	University of Kentucky
Sample_contact_address	800 Rose St.
Sample_contact_city	Lexington
Sample_contact_state	KY
Sample_contact_zip/postal_code	40475
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658098/suppl/GSM658098.CEL.gz
Sample_series_id	GSE26730
Sample_data_row_count	31099

GSM658099

GPL1355

granulosa cell.6h Ad-NFIL3 +FSK/PMA

ovarian granulosa cells collected from ovaries 48 h after stimulation with pregnant mare's serum gonadotropin

strain: Sprague-Dawley gender: Female age: Immature 24-25 days old

Sample_geo_accession	GSM658099
Sample_status	Public on Jan 20 2011
Sample_submission_date	Jan 19 2011
Sample_last_update_date	Jan 20 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The granulosa cells were exposed to Ad- NFIL3 or Ad-GFP at a multiplicity of infection (MOI) of 50 pfu/cell. Two hours later, Medium was replaced with fresh Opti-MEM medium. At 24 h after adenovirus exposure, granulosa cells were treated with FSK (10uM) + PMA (20 nM) for 6 h before collection for RNA isolation using a RNeasy kit according to the manufacturer’s instructions (Qiagen Inc., Valencia, CA).
Sample_growth_protocol_ch1	To isolate granulosa cells, ovaries were collected from rats 48 h after PMSG administration and processed as described previously. Briefly, granulosa cells were isolated by follicular puncture, pooled, filtered, pelleted by centrifugation at 200 x g for 5 min, and resuspended in Opti-MEM medium supplemented with 0.05 mg/ml of gentamycin and 1x ITS (insulin, transferin, and selenium). The cells were cultured in the absence or presence of various reagents discussed in detail below for different time points at 37oC in a humidified atmosphere of 5% CO2. Forskolin (FSK) and phorbol 12-myristate 13-acetate (PMA) were added to mimic hCG action.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Standard Affymetrix
Sample_label_ch1	Standard Affymetrix
Sample_label_protocol_ch1	Standard Affymetrix
Sample_hyb_protocol	Standard Affymetrix
Sample_scan_protocol	Standard Affymetrix
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Eric,M,Blalock
Sample_contact_email	emblal@uky.edu
Sample_contact_phone	859-323-8033
Sample_contact_laboratory	Blalock
Sample_contact_department	Molecular and Biomedical Pharmacology
Sample_contact_institute	University of Kentucky
Sample_contact_address	800 Rose St.
Sample_contact_city	Lexington
Sample_contact_state	KY
Sample_contact_zip/postal_code	40475
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658099/suppl/GSM658099.CEL.gz
Sample_series_id	GSE26730
Sample_data_row_count	31099

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