Result

Search results for the GEO ID: GSE26750

(Click on the check boxes provided under "Select for analysis", to initiate grouping)

(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down)

GSM ID

GPL ID

Select for analysis

Title

Source name

Description

Characteristics

GSM658325

GPL1261

Granule cell layer animal1-1

Cerebellum of 8-week-old C57BL/6J male

strain: C57BL/6J gender: male age: 8-week-old tissue: Cerebellum cell type: cerebellar granule cell layer

Gene expression data from mouse cerebellar granule cell layer

Sample_geo_accession	GSM658325
Sample_status	Public on Jan 17 2013
Sample_submission_date	Jan 20 2011
Sample_last_update_date	Jan 17 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Brains were rapidly removed and frozen in liquid nitrogen-chilled isopentane, and then stored at -80 ºC. Brains were sagittally sectioned at 12 μm thickness using cryostat at -20 ºC. Sections were fixed with a mixture of ethanol/acetic acid (19:1) on dry ice and dried out. Cells were cut using laser capture microdissection
Sample_growth_protocol_ch1	Intact 8-week-old mice were used
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were dissolved in RNA extraction buffer and stored at -80 ºC according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cDNAs were synthesized from mRNAs in approximately 1 ng of total RNA, amplified and labeled, according to standard protocols of Affymetrix GeneChip Expression Analysis (GeneChip Two-Cycle Target Labeling, Control Reagents, Affymetrix).
Sample_hyb_protocol	The samples were hybridized to DNA microarray, according to standard protocols of Affymetrix GeneChip Expression Analysis (Mouse Genome 430 2.0 Array, GeneChip Hybridization, Wash and Stain Kit, Affymetrix).
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	A dataset of each sample was analyzed with GeneChip Operating Software (GCOS 1, Affymetrix). The present (P), marginal (M), or absent (A) call for each data point was calculated by the standard algorithm. Each probe set contained 11 probes. Here, we chose 26,351 probe sets, corresponding to 17,197 genes, each of which contained more than nine probes matched to the defined cDNAs having gene symbols in the ENSEMBL or NCBI (reference sequence) database, and used them for further analysis.
Sample_platform_id	GPL1261
Sample_contact_name	Nozomu,H,Nakamura
Sample_contact_email	nakamun@gmail.com
Sample_contact_department	Molecular Neuroscience Unit
Sample_contact_institute	Okinawa Institute of Science and Technology
Sample_contact_address	12-22 Suzaki
Sample_contact_city	Uruma
Sample_contact_state	Okinawa
Sample_contact_zip/postal_code	904-2234
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658325/suppl/GSM658325.CEL.gz
Sample_series_id	GSE26750
Sample_series_id	GSE26751
Sample_data_row_count	45101

GSM658326

GPL1261

Granule cell layer animal1-2

Cerebellum of 8-week-old C57BL/6J male

strain: C57BL/6J gender: male age: 8-week-old tissue: Cerebellum cell type: cerebellar granule cell layer

Gene expression data from mouse cerebellar granule cell layer

Sample_geo_accession	GSM658326
Sample_status	Public on Jan 17 2013
Sample_submission_date	Jan 20 2011
Sample_last_update_date	Jan 17 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Brains were rapidly removed and frozen in liquid nitrogen-chilled isopentane, and then stored at -80 ºC. Brains were sagittally sectioned at 12 μm thickness using cryostat at -20 ºC. Sections were fixed with a mixture of ethanol/acetic acid (19:1) on dry ice and dried out. Cells were cut using laser capture microdissection
Sample_growth_protocol_ch1	Intact 8-week-old mice were used
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were dissolved in RNA extraction buffer and stored at -80 ºC according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cDNAs were synthesized from mRNAs in approximately 1 ng of total RNA, amplified and labeled, according to standard protocols of Affymetrix GeneChip Expression Analysis (GeneChip Two-Cycle Target Labeling, Control Reagents, Affymetrix).
Sample_hyb_protocol	The samples were hybridized to DNA microarray, according to standard protocols of Affymetrix GeneChip Expression Analysis (Mouse Genome 430 2.0 Array, GeneChip Hybridization, Wash and Stain Kit, Affymetrix).
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	A dataset of each sample was analyzed with GeneChip Operating Software (GCOS 1, Affymetrix). The present (P), marginal (M), or absent (A) call for each data point was calculated by the standard algorithm. Each probe set contained 11 probes. Here, we chose 26,351 probe sets, corresponding to 17,197 genes, each of which contained more than nine probes matched to the defined cDNAs having gene symbols in the ENSEMBL or NCBI (reference sequence) database, and used them for further analysis.
Sample_platform_id	GPL1261
Sample_contact_name	Nozomu,H,Nakamura
Sample_contact_email	nakamun@gmail.com
Sample_contact_department	Molecular Neuroscience Unit
Sample_contact_institute	Okinawa Institute of Science and Technology
Sample_contact_address	12-22 Suzaki
Sample_contact_city	Uruma
Sample_contact_state	Okinawa
Sample_contact_zip/postal_code	904-2234
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658326/suppl/GSM658326.CEL.gz
Sample_series_id	GSE26750
Sample_series_id	GSE26751
Sample_data_row_count	45101

Make groups for comparisons

(2 groups will be compared at a time)

Select GSMs and click on "Add groups"

Enter the group name here:

Select expression type

Transcripts profile based on;

A. Differential status (Up/Down regulation)

B. Absolute calls (Transcribed/Not-detected)

Filter results by number of probes