Search results for the GEO ID: GSE26771  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM658893 | GPL1261 |
|
MMTV-rtTA biological replicate 1 (TV01M001)
|
Control mammary epithelial cells, Doxycycline treated 7 days
|
strain: FVB/N
genotype/variation: MMTV-rtTA
gender: female
|
None
|
| Sample_geo_accession | GSM658893
| | Sample_status | Public on Jan 22 2011
| | Sample_submission_date | Jan 20 2011
| | Sample_last_update_date | Jan 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | mammary epithelial cells were isolated as previously described (McHenry PR et al., 2010, Breast Cancer Research)
| | Sample_growth_protocol_ch1 | 5 week old female mice were treated for 7 days with doxycycline containing chow (2 g/kg)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by purification using a RNeasy column according to manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tracy,,Vargo-Gogola
| | Sample_contact_phone | 574-631-1587
| | Sample_contact_fax | 574-631-7821
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1234 Notre Dame Avenue
| | Sample_contact_city | South Bend
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46617
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658893/suppl/GSM658893_TV01M001.CEL.gz
| | Sample_series_id | GSE26771
| | Sample_data_row_count | 45101
| | |
|
GSM658894 | GPL1261 |
|
MMTV-rtTA biological replicate 2 (TV01M002)
|
Control mammary epithelial cells, Doxycycline treated 7 days
|
strain: FVB/N
genotype/variation: MMTV-rtTA
gender: female
|
None
|
| Sample_geo_accession | GSM658894
| | Sample_status | Public on Jan 22 2011
| | Sample_submission_date | Jan 20 2011
| | Sample_last_update_date | Jan 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | mammary epithelial cells were isolated as previously described (McHenry PR et al., 2010, Breast Cancer Research)
| | Sample_growth_protocol_ch1 | 5 week old female mice were treated for 7 days with doxycycline containing chow (2 g/kg)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by purification using a RNeasy column according to manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tracy,,Vargo-Gogola
| | Sample_contact_phone | 574-631-1587
| | Sample_contact_fax | 574-631-7821
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1234 Notre Dame Avenue
| | Sample_contact_city | South Bend
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46617
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658894/suppl/GSM658894_TV01M002.CEL.gz
| | Sample_series_id | GSE26771
| | Sample_data_row_count | 45101
| | |
|
GSM658895 | GPL1261 |
|
MMTV-rtTA biological replicate 3 (TV01M003)
|
Control mammary epithelial cells, Doxycycline treated 7 days
|
strain: FVB/N
genotype/variation: MMTV-rtTA
gender: female
|
None
|
| Sample_geo_accession | GSM658895
| | Sample_status | Public on Jan 22 2011
| | Sample_submission_date | Jan 20 2011
| | Sample_last_update_date | Jan 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | mammary epithelial cells were isolated as previously described (McHenry PR et al., 2010, Breast Cancer Research)
| | Sample_growth_protocol_ch1 | 5 week old female mice were treated for 7 days with doxycycline containing chow (2 g/kg)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by purification using a RNeasy column according to manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tracy,,Vargo-Gogola
| | Sample_contact_phone | 574-631-1587
| | Sample_contact_fax | 574-631-7821
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1234 Notre Dame Avenue
| | Sample_contact_city | South Bend
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46617
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658895/suppl/GSM658895_TV01M003.CEL.gz
| | Sample_series_id | GSE26771
| | Sample_data_row_count | 45101
| | |
|
GSM658896 | GPL1261 |
|
MMTV-rtTA biological replicate 4 (TV01M004)
|
Control mammary epithelial cells, Doxycycline treated 7 days
|
strain: FVB/N
genotype/variation: MMTV-rtTA
gender: female
|
None
|
| Sample_geo_accession | GSM658896
| | Sample_status | Public on Jan 22 2011
| | Sample_submission_date | Jan 20 2011
| | Sample_last_update_date | Jan 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | mammary epithelial cells were isolated as previously described (McHenry PR et al., 2010, Breast Cancer Research)
| | Sample_growth_protocol_ch1 | 5 week old female mice were treated for 7 days with doxycycline containing chow (2 g/kg)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by purification using a RNeasy column according to manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tracy,,Vargo-Gogola
| | Sample_contact_phone | 574-631-1587
| | Sample_contact_fax | 574-631-7821
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1234 Notre Dame Avenue
| | Sample_contact_city | South Bend
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46617
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658896/suppl/GSM658896_TV01M004.CEL.gz
| | Sample_series_id | GSE26771
| | Sample_data_row_count | 45101
| | |
|
GSM658897 | GPL1261 |
|
MMTV-rtTA biological replicate 5 (TV01M005)
|
Control mammary epithelial cells, Doxycycline treated 7 days
|
strain: FVB/N
genotype/variation: MMTV-rtTA
gender: female
|
None
|
| Sample_geo_accession | GSM658897
| | Sample_status | Public on Jan 22 2011
| | Sample_submission_date | Jan 20 2011
| | Sample_last_update_date | Jan 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | mammary epithelial cells were isolated as previously described (McHenry PR et al., 2010, Breast Cancer Research)
| | Sample_growth_protocol_ch1 | 5 week old female mice were treated for 7 days with doxycycline containing chow (2 g/kg)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by purification using a RNeasy column according to manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tracy,,Vargo-Gogola
| | Sample_contact_phone | 574-631-1587
| | Sample_contact_fax | 574-631-7821
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1234 Notre Dame Avenue
| | Sample_contact_city | South Bend
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46617
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658897/suppl/GSM658897_TV01M005.CEL.gz
| | Sample_series_id | GSE26771
| | Sample_data_row_count | 45101
| | |
|
GSM658898 | GPL1261 |
|
MMTV-rtTA biological replicate 6 (TV01M006)
|
Control mammary epithelial cells, Doxycycline treated 7 days
|
strain: FVB/N
genotype/variation: MMTV-rtTA
gender: female
|
None
|
| Sample_geo_accession | GSM658898
| | Sample_status | Public on Jan 22 2011
| | Sample_submission_date | Jan 20 2011
| | Sample_last_update_date | Jan 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | mammary epithelial cells were isolated as previously described (McHenry PR et al., 2010, Breast Cancer Research)
| | Sample_growth_protocol_ch1 | 5 week old female mice were treated for 7 days with doxycycline containing chow (2 g/kg)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by purification using a RNeasy column according to manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tracy,,Vargo-Gogola
| | Sample_contact_phone | 574-631-1587
| | Sample_contact_fax | 574-631-7821
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1234 Notre Dame Avenue
| | Sample_contact_city | South Bend
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46617
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658898/suppl/GSM658898_TV01M006.CEL.gz
| | Sample_series_id | GSE26771
| | Sample_data_row_count | 45101
| | |
|
GSM658899 | GPL1261 |
|
p190B transgenic biological replicate 1 (TV01M007)
|
p190B expressing mammary epithelial cells, Doxycycline treated 7 days
|
strain: FVB/N
genotype/variation: MMTV-rtTA/TetO-p190B
gender: female
|
None
|
| Sample_geo_accession | GSM658899
| | Sample_status | Public on Jan 22 2011
| | Sample_submission_date | Jan 20 2011
| | Sample_last_update_date | Jan 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | mammary epithelial cells were isolated as previously described (McHenry PR et al., 2010, Breast Cancer Research)
| | Sample_growth_protocol_ch1 | 5 week old female mice were treated for 7 days with doxycycline containing chow (2 g/kg)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by purification using a RNeasy column according to manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tracy,,Vargo-Gogola
| | Sample_contact_phone | 574-631-1587
| | Sample_contact_fax | 574-631-7821
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1234 Notre Dame Avenue
| | Sample_contact_city | South Bend
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46617
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658899/suppl/GSM658899_TV01M007.CEL.gz
| | Sample_series_id | GSE26771
| | Sample_data_row_count | 45101
| | |
|
GSM658900 | GPL1261 |
|
p190B transgenic biological replicate 2 (TV01M008)
|
p190B expressing mammary epithelial cells, Doxycycline treated 7 days
|
strain: FVB/N
genotype/variation: MMTV-rtTA/TetO-p190B
gender: female
|
None
|
| Sample_geo_accession | GSM658900
| | Sample_status | Public on Jan 22 2011
| | Sample_submission_date | Jan 20 2011
| | Sample_last_update_date | Jan 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | mammary epithelial cells were isolated as previously described (McHenry PR et al., 2010, Breast Cancer Research)
| | Sample_growth_protocol_ch1 | 5 week old female mice were treated for 7 days with doxycycline containing chow (2 g/kg)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by purification using a RNeasy column according to manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tracy,,Vargo-Gogola
| | Sample_contact_phone | 574-631-1587
| | Sample_contact_fax | 574-631-7821
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1234 Notre Dame Avenue
| | Sample_contact_city | South Bend
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46617
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658900/suppl/GSM658900_TV01M008.CEL.gz
| | Sample_series_id | GSE26771
| | Sample_data_row_count | 45101
| | |
|
GSM658901 | GPL1261 |
|
p190B transgenic biological replicate 3 (TV01M009)
|
p190B expressing mammary epithelial cells, Doxycycline treated 7 days
|
strain: FVB/N
genotype/variation: MMTV-rtTA/TetO-p190B
gender: female
|
None
|
| Sample_geo_accession | GSM658901
| | Sample_status | Public on Jan 22 2011
| | Sample_submission_date | Jan 20 2011
| | Sample_last_update_date | Jan 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | mammary epithelial cells were isolated as previously described (McHenry PR et al., 2010, Breast Cancer Research)
| | Sample_growth_protocol_ch1 | 5 week old female mice were treated for 7 days with doxycycline containing chow (2 g/kg)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by purification using a RNeasy column according to manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tracy,,Vargo-Gogola
| | Sample_contact_phone | 574-631-1587
| | Sample_contact_fax | 574-631-7821
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1234 Notre Dame Avenue
| | Sample_contact_city | South Bend
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46617
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658901/suppl/GSM658901_TV01M009.CEL.gz
| | Sample_series_id | GSE26771
| | Sample_data_row_count | 45101
| | |
|
GSM658902 | GPL1261 |
|
p190B transgenic biological replicate 4 (TV01M010)
|
p190B expressing mammary epithelial cells, Doxycycline treated 7 days
|
strain: FVB/N
genotype/variation: MMTV-rtTA/TetO-p190B
gender: female
|
None
|
| Sample_geo_accession | GSM658902
| | Sample_status | Public on Jan 22 2011
| | Sample_submission_date | Jan 20 2011
| | Sample_last_update_date | Jan 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | mammary epithelial cells were isolated as previously described (McHenry PR et al., 2010, Breast Cancer Research)
| | Sample_growth_protocol_ch1 | 5 week old female mice were treated for 7 days with doxycycline containing chow (2 g/kg)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by purification using a RNeasy column according to manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tracy,,Vargo-Gogola
| | Sample_contact_phone | 574-631-1587
| | Sample_contact_fax | 574-631-7821
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1234 Notre Dame Avenue
| | Sample_contact_city | South Bend
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46617
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658902/suppl/GSM658902_TV01M010.CEL.gz
| | Sample_series_id | GSE26771
| | Sample_data_row_count | 45101
| | |
|
GSM658903 | GPL1261 |
|
p190B transgenic biological replicate 5 (TV01M011)
|
p190B expressing mammary epithelial cells, Doxycycline treated 7 days
|
strain: FVB/N
genotype/variation: MMTV-rtTA/TetO-p190B
gender: female
|
None
|
| Sample_geo_accession | GSM658903
| | Sample_status | Public on Jan 22 2011
| | Sample_submission_date | Jan 20 2011
| | Sample_last_update_date | Jan 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | mammary epithelial cells were isolated as previously described (McHenry PR et al., 2010, Breast Cancer Research)
| | Sample_growth_protocol_ch1 | 5 week old female mice were treated for 7 days with doxycycline containing chow (2 g/kg)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by purification using a RNeasy column according to manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tracy,,Vargo-Gogola
| | Sample_contact_phone | 574-631-1587
| | Sample_contact_fax | 574-631-7821
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1234 Notre Dame Avenue
| | Sample_contact_city | South Bend
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46617
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658903/suppl/GSM658903_TV01M011.CEL.gz
| | Sample_series_id | GSE26771
| | Sample_data_row_count | 45101
| | |
|
GSM658904 | GPL1261 |
|
p190B transgenic biological replicate 6 (TV01M012)
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p190B expressing mammary epithelial cells, Doxycycline treated 7 days
|
strain: FVB/N
genotype/variation: MMTV-rtTA/TetO-p190B
gender: female
|
None
|
| Sample_geo_accession | GSM658904
| | Sample_status | Public on Jan 22 2011
| | Sample_submission_date | Jan 20 2011
| | Sample_last_update_date | Jan 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | mammary epithelial cells were isolated as previously described (McHenry PR et al., 2010, Breast Cancer Research)
| | Sample_growth_protocol_ch1 | 5 week old female mice were treated for 7 days with doxycycline containing chow (2 g/kg)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by purification using a RNeasy column according to manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on GeneChip RG-U34A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Tracy,,Vargo-Gogola
| | Sample_contact_phone | 574-631-1587
| | Sample_contact_fax | 574-631-7821
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1234 Notre Dame Avenue
| | Sample_contact_city | South Bend
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46617
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM658nnn/GSM658904/suppl/GSM658904_TV01M012.CEL.gz
| | Sample_series_id | GSE26771
| | Sample_data_row_count | 45101
| | |
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