Result

Search results for the GEO ID: GSE26822

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Title

Source name

Description

Characteristics

GSM659606

GPL1261

P16 mutant OC apex, biological rep 1

Mouse organ of Corti from cochlea, apical half, Dicer1 conditional knockout

mouse genome informatics (mgi) strains: Dicer1tm1Bdh (MGI:3589208) X Tg(Atoh1-cre)1Bfri (MGI:3775845) genotype: Dicer1 conditional knockout, Atoh1-Cre;Dicer1(flox/flox) tissue: organ of Corti from cochlea, apical half age: postnatal day 16

Gene expression data from mouse organ of Corti apex, Dicer1 conditional knockout

Sample_geo_accession	GSM659606
Sample_status	Public on Jan 01 2012
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Organ of Corti was immediately dissected from inner ear in phosphate-buffered saline, parted into apical and basal sections, and placed in lysis buffer on ice for immediate RNA extraction.
Sample_growth_protocol_ch1	Genotyped mice were aged to postnatal day 16 and euthanized according to IACUC approved protocol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	mirVana kit isolation of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	75 to 125 ng of total RNA was amplified and labeled using the Affymetrix 2-cycle labeling kit per the manufacturer's suggestions.
Sample_hyb_protocol	15 ug of biotinylated target was hybridized and stained per standard protocols per manufacturer's suggestions in the Affymetrix hybridization oven model 640 and fluidics station model 450.
Sample_scan_protocol	The arrays were scanned using an Affymetrix High Resolution scanner 3000 7G.
Sample_data_processing	The data were analyzed using Bioconductor (R) version 2.6.1 and the affy package version 1.26 using the mas5 function to obtain probe set expression values that correspond to those from MAS 5.0 Affymetrix default analysis settings and global scaling normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Garrett,,Soukup
Sample_contact_email	gasoukup@creighton.edu
Sample_contact_phone	402-280-5754
Sample_contact_department	Biomedical Sciences
Sample_contact_institute	Creighton University
Sample_contact_address	2500 California Plaza
Sample_contact_city	Omaha
Sample_contact_state	NE
Sample_contact_zip/postal_code	68178
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659606/suppl/GSM659606.CEL.gz
Sample_series_id	GSE26822
Sample_data_row_count	45101

GSM659607

GPL1261

P16 mutant OC apex, biological rep 2

Mouse organ of Corti from cochlea, apical half, Dicer1 conditional knockout

Gene expression data from mouse organ of Corti apex, Dicer1 conditional knockout

Sample_geo_accession	GSM659607
Sample_status	Public on Jan 01 2012
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Organ of Corti was immediately dissected from inner ear in phosphate-buffered saline, parted into apical and basal sections, and placed in lysis buffer on ice for immediate RNA extraction.
Sample_growth_protocol_ch1	Genotyped mice were aged to postnatal day 16 and euthanized according to IACUC approved protocol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	mirVana kit isolation of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	75 to 125 ng of total RNA was amplified and labeled using the Affymetrix 2-cycle labeling kit per the manufacturer's suggestions.
Sample_hyb_protocol	15 ug of biotinylated target was hybridized and stained per standard protocols per manufacturer's suggestions in the Affymetrix hybridization oven model 640 and fluidics station model 450.
Sample_scan_protocol	The arrays were scanned using an Affymetrix High Resolution scanner 3000 7G.
Sample_data_processing	The data were analyzed using Bioconductor (R) version 2.6.1 and the affy package version 1.26 using the mas5 function to obtain probe set expression values that correspond to those from MAS 5.0 Affymetrix default analysis settings and global scaling normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Garrett,,Soukup
Sample_contact_email	gasoukup@creighton.edu
Sample_contact_phone	402-280-5754
Sample_contact_department	Biomedical Sciences
Sample_contact_institute	Creighton University
Sample_contact_address	2500 California Plaza
Sample_contact_city	Omaha
Sample_contact_state	NE
Sample_contact_zip/postal_code	68178
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659607/suppl/GSM659607.CEL.gz
Sample_series_id	GSE26822
Sample_data_row_count	45101

GSM659608

GPL1261

P16 mutant OC base, biological rep 1

Mouse organ of Corti from cochlea, basal half, Dicer1 conditional knockout

Gene expression data from mouse organ of Corti base, Dicer1 conditional knockout

Sample_geo_accession	GSM659608
Sample_status	Public on Jan 01 2012
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Organ of Corti was immediately dissected from inner ear in phosphate-buffered saline, parted into apical and basal sections, and placed in lysis buffer on ice for immediate RNA extraction.
Sample_growth_protocol_ch1	Genotyped mice were aged to postnatal day 16 and euthanized according to IACUC approved protocol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	mirVana kit isolation of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	75 to 125 ng of total RNA was amplified and labeled using the Affymetrix 2-cycle labeling kit per the manufacturer's suggestions.
Sample_hyb_protocol	15 ug of biotinylated target was hybridized and stained per standard protocols per manufacturer's suggestions in the Affymetrix hybridization oven model 640 and fluidics station model 450.
Sample_scan_protocol	The arrays were scanned using an Affymetrix High Resolution scanner 3000 7G.
Sample_data_processing	The data were analyzed using Bioconductor (R) version 2.6.1 and the affy package version 1.26 using the mas5 function to obtain probe set expression values that correspond to those from MAS 5.0 Affymetrix default analysis settings and global scaling normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Garrett,,Soukup
Sample_contact_email	gasoukup@creighton.edu
Sample_contact_phone	402-280-5754
Sample_contact_department	Biomedical Sciences
Sample_contact_institute	Creighton University
Sample_contact_address	2500 California Plaza
Sample_contact_city	Omaha
Sample_contact_state	NE
Sample_contact_zip/postal_code	68178
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659608/suppl/GSM659608.CEL.gz
Sample_series_id	GSE26822
Sample_data_row_count	45101

GSM659609

GPL1261

P16 mutant OC base, biological rep 2

Mouse organ of Corti from cochlea, basal half, Dicer1 conditional knockout

Gene expression data from mouse organ of Corti base, Dicer1 conditional knockout

Sample_geo_accession	GSM659609
Sample_status	Public on Jan 01 2012
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Organ of Corti was immediately dissected from inner ear in phosphate-buffered saline, parted into apical and basal sections, and placed in lysis buffer on ice for immediate RNA extraction.
Sample_growth_protocol_ch1	Genotyped mice were aged to postnatal day 16 and euthanized according to IACUC approved protocol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	mirVana kit isolation of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	75 to 125 ng of total RNA was amplified and labeled using the Affymetrix 2-cycle labeling kit per the manufacturer's suggestions.
Sample_hyb_protocol	15 ug of biotinylated target was hybridized and stained per standard protocols per manufacturer's suggestions in the Affymetrix hybridization oven model 640 and fluidics station model 450.
Sample_scan_protocol	The arrays were scanned using an Affymetrix High Resolution scanner 3000 7G.
Sample_data_processing	The data were analyzed using Bioconductor (R) version 2.6.1 and the affy package version 1.26 using the mas5 function to obtain probe set expression values that correspond to those from MAS 5.0 Affymetrix default analysis settings and global scaling normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Garrett,,Soukup
Sample_contact_email	gasoukup@creighton.edu
Sample_contact_phone	402-280-5754
Sample_contact_department	Biomedical Sciences
Sample_contact_institute	Creighton University
Sample_contact_address	2500 California Plaza
Sample_contact_city	Omaha
Sample_contact_state	NE
Sample_contact_zip/postal_code	68178
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659609/suppl/GSM659609.CEL.gz
Sample_series_id	GSE26822
Sample_data_row_count	45101

GSM659610

GPL1261

P16 control OC apex, biological rep 1

Mouse organ of Corti from cochlea, apical half, littermate control

mouse genome informatics (mgi) strains: Dicer1tm1Bdh (MGI:3589208) X Tg(Atoh1-cre)1Bfri (MGI:3775845) genotype: littermate control, Atoh1-Cre;Dicer1(flox/wt) tissue: organ of Corti from cochlea, apical half age: postnatal day 16

Gene expression data from mouse organ of Corti apex, littermate control

Sample_geo_accession	GSM659610
Sample_status	Public on Jan 01 2012
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Organ of Corti was immediately dissected from inner ear in phosphate-buffered saline, parted into apical and basal sections, and placed in lysis buffer on ice for immediate RNA extraction.
Sample_growth_protocol_ch1	Genotyped mice were aged to postnatal day 16 and euthanized according to IACUC approved protocol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	mirVana kit isolation of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	75 to 125 ng of total RNA was amplified and labeled using the Affymetrix 2-cycle labeling kit per the manufacturer's suggestions.
Sample_hyb_protocol	15 ug of biotinylated target was hybridized and stained per standard protocols per manufacturer's suggestions in the Affymetrix hybridization oven model 640 and fluidics station model 450.
Sample_scan_protocol	The arrays were scanned using an Affymetrix High Resolution scanner 3000 7G.
Sample_data_processing	The data were analyzed using Bioconductor (R) version 2.6.1 and the affy package version 1.26 using the mas5 function to obtain probe set expression values that correspond to those from MAS 5.0 Affymetrix default analysis settings and global scaling normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Garrett,,Soukup
Sample_contact_email	gasoukup@creighton.edu
Sample_contact_phone	402-280-5754
Sample_contact_department	Biomedical Sciences
Sample_contact_institute	Creighton University
Sample_contact_address	2500 California Plaza
Sample_contact_city	Omaha
Sample_contact_state	NE
Sample_contact_zip/postal_code	68178
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659610/suppl/GSM659610.CEL.gz
Sample_series_id	GSE26822
Sample_data_row_count	45101

GSM659611

GPL1261

P16 control OC apex, biological rep 2

Mouse organ of Corti from cochlea, apical half, littermate control

Gene expression data from mouse organ of Corti apex, littermate control

Sample_geo_accession	GSM659611
Sample_status	Public on Jan 01 2012
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Organ of Corti was immediately dissected from inner ear in phosphate-buffered saline, parted into apical and basal sections, and placed in lysis buffer on ice for immediate RNA extraction.
Sample_growth_protocol_ch1	Genotyped mice were aged to postnatal day 16 and euthanized according to IACUC approved protocol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	mirVana kit isolation of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	75 to 125 ng of total RNA was amplified and labeled using the Affymetrix 2-cycle labeling kit per the manufacturer's suggestions.
Sample_hyb_protocol	15 ug of biotinylated target was hybridized and stained per standard protocols per manufacturer's suggestions in the Affymetrix hybridization oven model 640 and fluidics station model 450.
Sample_scan_protocol	The arrays were scanned using an Affymetrix High Resolution scanner 3000 7G.
Sample_data_processing	The data were analyzed using Bioconductor (R) version 2.6.1 and the affy package version 1.26 using the mas5 function to obtain probe set expression values that correspond to those from MAS 5.0 Affymetrix default analysis settings and global scaling normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Garrett,,Soukup
Sample_contact_email	gasoukup@creighton.edu
Sample_contact_phone	402-280-5754
Sample_contact_department	Biomedical Sciences
Sample_contact_institute	Creighton University
Sample_contact_address	2500 California Plaza
Sample_contact_city	Omaha
Sample_contact_state	NE
Sample_contact_zip/postal_code	68178
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659611/suppl/GSM659611.CEL.gz
Sample_series_id	GSE26822
Sample_data_row_count	45101

GSM659612

GPL1261

P16 control OC base, biological rep 1

Mouse organ of Corti from cochlea, basal half, littermate control

Gene expression data from mouse organ of Corti base, littermate control

Sample_geo_accession	GSM659612
Sample_status	Public on Jan 01 2012
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Organ of Corti was immediately dissected from inner ear in phosphate-buffered saline, parted into apical and basal sections, and placed in lysis buffer on ice for immediate RNA extraction.
Sample_growth_protocol_ch1	Genotyped mice were aged to postnatal day 16 and euthanized according to IACUC approved protocol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	mirVana kit isolation of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	75 to 125 ng of total RNA was amplified and labeled using the Affymetrix 2-cycle labeling kit per the manufacturer's suggestions.
Sample_hyb_protocol	15 ug of biotinylated target was hybridized and stained per standard protocols per manufacturer's suggestions in the Affymetrix hybridization oven model 640 and fluidics station model 450.
Sample_scan_protocol	The arrays were scanned using an Affymetrix High Resolution scanner 3000 7G.
Sample_data_processing	The data were analyzed using Bioconductor (R) version 2.6.1 and the affy package version 1.26 using the mas5 function to obtain probe set expression values that correspond to those from MAS 5.0 Affymetrix default analysis settings and global scaling normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Garrett,,Soukup
Sample_contact_email	gasoukup@creighton.edu
Sample_contact_phone	402-280-5754
Sample_contact_department	Biomedical Sciences
Sample_contact_institute	Creighton University
Sample_contact_address	2500 California Plaza
Sample_contact_city	Omaha
Sample_contact_state	NE
Sample_contact_zip/postal_code	68178
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659612/suppl/GSM659612.CEL.gz
Sample_series_id	GSE26822
Sample_data_row_count	45101

GSM659613

GPL1261

P16 control OC base, biological rep 2

Mouse organ of Corti from cochlea, basal half, littermate control

Gene expression data from mouse organ of Corti base, littermate control

Sample_geo_accession	GSM659613
Sample_status	Public on Jan 01 2012
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Organ of Corti was immediately dissected from inner ear in phosphate-buffered saline, parted into apical and basal sections, and placed in lysis buffer on ice for immediate RNA extraction.
Sample_growth_protocol_ch1	Genotyped mice were aged to postnatal day 16 and euthanized according to IACUC approved protocol.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	mirVana kit isolation of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	75 to 125 ng of total RNA was amplified and labeled using the Affymetrix 2-cycle labeling kit per the manufacturer's suggestions.
Sample_hyb_protocol	15 ug of biotinylated target was hybridized and stained per standard protocols per manufacturer's suggestions in the Affymetrix hybridization oven model 640 and fluidics station model 450.
Sample_scan_protocol	The arrays were scanned using an Affymetrix High Resolution scanner 3000 7G.
Sample_data_processing	The data were analyzed using Bioconductor (R) version 2.6.1 and the affy package version 1.26 using the mas5 function to obtain probe set expression values that correspond to those from MAS 5.0 Affymetrix default analysis settings and global scaling normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Garrett,,Soukup
Sample_contact_email	gasoukup@creighton.edu
Sample_contact_phone	402-280-5754
Sample_contact_department	Biomedical Sciences
Sample_contact_institute	Creighton University
Sample_contact_address	2500 California Plaza
Sample_contact_city	Omaha
Sample_contact_state	NE
Sample_contact_zip/postal_code	68178
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659613/suppl/GSM659613.CEL.gz
Sample_series_id	GSE26822
Sample_data_row_count	45101

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