Result

Search results for the GEO ID: GSE26828

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM659708

GPL570

Control_1

UROtsa cell, control

cell line: UROtsa agent: Control

Gene expression data from UROtsa cells

Sample_geo_accession	GSM659708
Sample_status	Public on Jan 25 2011
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	1 micromolar cadmium as cadmium chloride was added to the growth medium and the cells were passaged weekly until the cells attained the ability to form colonies in soft agar.
Sample_growth_protocol_ch1	Cells grown in Dulbeco's modified Eagles medium supplemented with 5% FCS. Split ratio was 1-4 weekly.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested in normal medium without cadmium and the RNA purified using the Rneasy Kit from Qiagen.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was biotinylated using the Bioarray cDNA Synthesis kit (ENZO Diagnostics, Farmingdale, NY)
Sample_hyb_protocol	15 ug of fragmented labeled RNA was hybridized for 16 hr at 45 degree C to an Affymetrix U133 Plus 2.0 array. Arrays were wased and stained using a Fluidics Station 450 according to the manufacturer's instructions. Arrays were stained with phycoerythrein-conjugated streptavidin.
Sample_scan_protocol	The fluorescence intensities were determined using a GCS 3000 7G high-resolution confocal laser scanner from Affymetrix.
Sample_data_processing	Images were analyzed using programs resident in GeneChip Operating System v1.4 (GCOS; Affymetrix). Quality control metrics for cRNA integrity, sample loading, and variations in staining were determined after background correction and signal summarization by MAS 5.0. The probes whose MAS5 Detection Call=Absent for all 9 samples were filtered out prior to statistical analysis, and thus were not included in the data table.
Sample_platform_id	GPL570
Sample_contact_name	Donald,,Sens
Sample_contact_email	donald.sens@med.und.edu
Sample_contact_phone	701-777-6258
Sample_contact_fax	701-777-3108
Sample_contact_department	Pathology
Sample_contact_institute	University of North Dakota
Sample_contact_address	501 North Columbia Road
Sample_contact_city	Grand Forks
Sample_contact_state	ND
Sample_contact_zip/postal_code	59202-9037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659708/suppl/GSM659708.CEL.gz
Sample_series_id	GSE26828
Sample_data_row_count	26877

GSM659709

GPL570

Control_2

UROtsa cell, control

cell line: UROtsa agent: Control

Gene expression data from UROtsa cells

Sample_geo_accession	GSM659709
Sample_status	Public on Jan 25 2011
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	1 micromolar cadmium as cadmium chloride was added to the growth medium and the cells were passaged weekly until the cells attained the ability to form colonies in soft agar.
Sample_growth_protocol_ch1	Cells grown in Dulbeco's modified Eagles medium supplemented with 5% FCS. Split ratio was 1-4 weekly.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested in normal medium without cadmium and the RNA purified using the Rneasy Kit from Qiagen.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was biotinylated using the Bioarray cDNA Synthesis kit (ENZO Diagnostics, Farmingdale, NY)
Sample_hyb_protocol	15 ug of fragmented labeled RNA was hybridized for 16 hr at 45 degree C to an Affymetrix U133 Plus 2.0 array. Arrays were wased and stained using a Fluidics Station 450 according to the manufacturer's instructions. Arrays were stained with phycoerythrein-conjugated streptavidin.
Sample_scan_protocol	The fluorescence intensities were determined using a GCS 3000 7G high-resolution confocal laser scanner from Affymetrix.
Sample_data_processing	Images were analyzed using programs resident in GeneChip Operating System v1.4 (GCOS; Affymetrix). Quality control metrics for cRNA integrity, sample loading, and variations in staining were determined after background correction and signal summarization by MAS 5.0. The probes whose MAS5 Detection Call=Absent for all 9 samples were filtered out prior to statistical analysis, and thus were not included in the data table.
Sample_platform_id	GPL570
Sample_contact_name	Donald,,Sens
Sample_contact_email	donald.sens@med.und.edu
Sample_contact_phone	701-777-6258
Sample_contact_fax	701-777-3108
Sample_contact_department	Pathology
Sample_contact_institute	University of North Dakota
Sample_contact_address	501 North Columbia Road
Sample_contact_city	Grand Forks
Sample_contact_state	ND
Sample_contact_zip/postal_code	59202-9037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659709/suppl/GSM659709.CEL.gz
Sample_series_id	GSE26828
Sample_data_row_count	26877

GSM659710

GPL570

Control_3

UROtsa cell, control

cell line: UROtsa agent: Control

Gene expression data from UROtsa cells

Sample_geo_accession	GSM659710
Sample_status	Public on Jan 25 2011
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	1 micromolar cadmium as cadmium chloride was added to the growth medium and the cells were passaged weekly until the cells attained the ability to form colonies in soft agar.
Sample_growth_protocol_ch1	Cells grown in Dulbeco's modified Eagles medium supplemented with 5% FCS. Split ratio was 1-4 weekly.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested in normal medium without cadmium and the RNA purified using the Rneasy Kit from Qiagen.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was biotinylated using the Bioarray cDNA Synthesis kit (ENZO Diagnostics, Farmingdale, NY)
Sample_hyb_protocol	15 ug of fragmented labeled RNA was hybridized for 16 hr at 45 degree C to an Affymetrix U133 Plus 2.0 array. Arrays were wased and stained using a Fluidics Station 450 according to the manufacturer's instructions. Arrays were stained with phycoerythrein-conjugated streptavidin.
Sample_scan_protocol	The fluorescence intensities were determined using a GCS 3000 7G high-resolution confocal laser scanner from Affymetrix.
Sample_data_processing	Images were analyzed using programs resident in GeneChip Operating System v1.4 (GCOS; Affymetrix). Quality control metrics for cRNA integrity, sample loading, and variations in staining were determined after background correction and signal summarization by MAS 5.0. The probes whose MAS5 Detection Call=Absent for all 9 samples were filtered out prior to statistical analysis, and thus were not included in the data table.
Sample_platform_id	GPL570
Sample_contact_name	Donald,,Sens
Sample_contact_email	donald.sens@med.und.edu
Sample_contact_phone	701-777-6258
Sample_contact_fax	701-777-3108
Sample_contact_department	Pathology
Sample_contact_institute	University of North Dakota
Sample_contact_address	501 North Columbia Road
Sample_contact_city	Grand Forks
Sample_contact_state	ND
Sample_contact_zip/postal_code	59202-9037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659710/suppl/GSM659710.CEL.gz
Sample_series_id	GSE26828
Sample_data_row_count	26877

GSM659711

GPL570

UROtsa_Cd_2

UROtsa cell, Cadmium-transformed

cell line: UROtsa agent: Cadmium

Gene expression data from cadmium-transformed UROtsa cells

Sample_geo_accession	GSM659711
Sample_status	Public on Jan 25 2011
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	1 micromolar cadmium as cadmium chloride was added to the growth medium and the cells were passaged weekly until the cells attained the ability to form colonies in soft agar.
Sample_growth_protocol_ch1	Cells grown in Dulbeco's modified Eagles medium supplemented with 5% FCS. Split ratio was 1-4 weekly.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested in normal medium without cadmium and the RNA purified using the Rneasy Kit from Qiagen.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was biotinylated using the Bioarray cDNA Synthesis kit (ENZO Diagnostics, Farmingdale, NY)
Sample_hyb_protocol	15 ug of fragmented labeled RNA was hybridized for 16 hr at 45 degree C to an Affymetrix U133 Plus 2.0 array. Arrays were wased and stained using a Fluidics Station 450 according to the manufacturer's instructions. Arrays were stained with phycoerythrein-conjugated streptavidin.
Sample_scan_protocol	The fluorescence intensities were determined using a GCS 3000 7G high-resolution confocal laser scanner from Affymetrix.
Sample_data_processing	Images were analyzed using programs resident in GeneChip Operating System v1.4 (GCOS; Affymetrix). Quality control metrics for cRNA integrity, sample loading, and variations in staining were determined after background correction and signal summarization by MAS 5.0. The probes whose MAS5 Detection Call=Absent for all 9 samples were filtered out prior to statistical analysis, and thus were not included in the data table.
Sample_platform_id	GPL570
Sample_contact_name	Donald,,Sens
Sample_contact_email	donald.sens@med.und.edu
Sample_contact_phone	701-777-6258
Sample_contact_fax	701-777-3108
Sample_contact_department	Pathology
Sample_contact_institute	University of North Dakota
Sample_contact_address	501 North Columbia Road
Sample_contact_city	Grand Forks
Sample_contact_state	ND
Sample_contact_zip/postal_code	59202-9037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659711/suppl/GSM659711.CEL.gz
Sample_series_id	GSE26828
Sample_data_row_count	26877

GSM659712

GPL570

UROtsa_Cd_3

UROtsa cell, Cadmium-transformed

cell line: UROtsa agent: Cadmium

Gene expression data from cadmium-transformed UROtsa cells

Sample_geo_accession	GSM659712
Sample_status	Public on Jan 25 2011
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	1 micromolar cadmium as cadmium chloride was added to the growth medium and the cells were passaged weekly until the cells attained the ability to form colonies in soft agar.
Sample_growth_protocol_ch1	Cells grown in Dulbeco's modified Eagles medium supplemented with 5% FCS. Split ratio was 1-4 weekly.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested in normal medium without cadmium and the RNA purified using the Rneasy Kit from Qiagen.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was biotinylated using the Bioarray cDNA Synthesis kit (ENZO Diagnostics, Farmingdale, NY)
Sample_hyb_protocol	15 ug of fragmented labeled RNA was hybridized for 16 hr at 45 degree C to an Affymetrix U133 Plus 2.0 array. Arrays were wased and stained using a Fluidics Station 450 according to the manufacturer's instructions. Arrays were stained with phycoerythrein-conjugated streptavidin.
Sample_scan_protocol	The fluorescence intensities were determined using a GCS 3000 7G high-resolution confocal laser scanner from Affymetrix.
Sample_data_processing	Images were analyzed using programs resident in GeneChip Operating System v1.4 (GCOS; Affymetrix). Quality control metrics for cRNA integrity, sample loading, and variations in staining were determined after background correction and signal summarization by MAS 5.0. The probes whose MAS5 Detection Call=Absent for all 9 samples were filtered out prior to statistical analysis, and thus were not included in the data table.
Sample_platform_id	GPL570
Sample_contact_name	Donald,,Sens
Sample_contact_email	donald.sens@med.und.edu
Sample_contact_phone	701-777-6258
Sample_contact_fax	701-777-3108
Sample_contact_department	Pathology
Sample_contact_institute	University of North Dakota
Sample_contact_address	501 North Columbia Road
Sample_contact_city	Grand Forks
Sample_contact_state	ND
Sample_contact_zip/postal_code	59202-9037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659712/suppl/GSM659712.CEL.gz
Sample_series_id	GSE26828
Sample_data_row_count	26877

GSM659713

GPL570

UROtsa_Cd_4

UROtsa cell, Cadmium-transformed

cell line: UROtsa agent: Cadmium

Gene expression data from cadmium-transformed UROtsa cells

Sample_geo_accession	GSM659713
Sample_status	Public on Jan 25 2011
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	1 micromolar cadmium as cadmium chloride was added to the growth medium and the cells were passaged weekly until the cells attained the ability to form colonies in soft agar.
Sample_growth_protocol_ch1	Cells grown in Dulbeco's modified Eagles medium supplemented with 5% FCS. Split ratio was 1-4 weekly.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested in normal medium without cadmium and the RNA purified using the Rneasy Kit from Qiagen.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was biotinylated using the Bioarray cDNA Synthesis kit (ENZO Diagnostics, Farmingdale, NY)
Sample_hyb_protocol	15 ug of fragmented labeled RNA was hybridized for 16 hr at 45 degree C to an Affymetrix U133 Plus 2.0 array. Arrays were wased and stained using a Fluidics Station 450 according to the manufacturer's instructions. Arrays were stained with phycoerythrein-conjugated streptavidin.
Sample_scan_protocol	The fluorescence intensities were determined using a GCS 3000 7G high-resolution confocal laser scanner from Affymetrix.
Sample_data_processing	Images were analyzed using programs resident in GeneChip Operating System v1.4 (GCOS; Affymetrix). Quality control metrics for cRNA integrity, sample loading, and variations in staining were determined after background correction and signal summarization by MAS 5.0. The probes whose MAS5 Detection Call=Absent for all 9 samples were filtered out prior to statistical analysis, and thus were not included in the data table.
Sample_platform_id	GPL570
Sample_contact_name	Donald,,Sens
Sample_contact_email	donald.sens@med.und.edu
Sample_contact_phone	701-777-6258
Sample_contact_fax	701-777-3108
Sample_contact_department	Pathology
Sample_contact_institute	University of North Dakota
Sample_contact_address	501 North Columbia Road
Sample_contact_city	Grand Forks
Sample_contact_state	ND
Sample_contact_zip/postal_code	59202-9037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659713/suppl/GSM659713.CEL.gz
Sample_series_id	GSE26828
Sample_data_row_count	26877

GSM659714

GPL570

UROtsa_Cd_5

UROtsa cell, Cadmium-transformed

cell line: UROtsa agent: Cadmium

Gene expression data from cadmium-transformed UROtsa cells

Sample_geo_accession	GSM659714
Sample_status	Public on Jan 25 2011
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	1 micromolar cadmium as cadmium chloride was added to the growth medium and the cells were passaged weekly until the cells attained the ability to form colonies in soft agar.
Sample_growth_protocol_ch1	Cells grown in Dulbeco's modified Eagles medium supplemented with 5% FCS. Split ratio was 1-4 weekly.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested in normal medium without cadmium and the RNA purified using the Rneasy Kit from Qiagen.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was biotinylated using the Bioarray cDNA Synthesis kit (ENZO Diagnostics, Farmingdale, NY)
Sample_hyb_protocol	15 ug of fragmented labeled RNA was hybridized for 16 hr at 45 degree C to an Affymetrix U133 Plus 2.0 array. Arrays were wased and stained using a Fluidics Station 450 according to the manufacturer's instructions. Arrays were stained with phycoerythrein-conjugated streptavidin.
Sample_scan_protocol	The fluorescence intensities were determined using a GCS 3000 7G high-resolution confocal laser scanner from Affymetrix.
Sample_data_processing	Images were analyzed using programs resident in GeneChip Operating System v1.4 (GCOS; Affymetrix). Quality control metrics for cRNA integrity, sample loading, and variations in staining were determined after background correction and signal summarization by MAS 5.0. The probes whose MAS5 Detection Call=Absent for all 9 samples were filtered out prior to statistical analysis, and thus were not included in the data table.
Sample_platform_id	GPL570
Sample_contact_name	Donald,,Sens
Sample_contact_email	donald.sens@med.und.edu
Sample_contact_phone	701-777-6258
Sample_contact_fax	701-777-3108
Sample_contact_department	Pathology
Sample_contact_institute	University of North Dakota
Sample_contact_address	501 North Columbia Road
Sample_contact_city	Grand Forks
Sample_contact_state	ND
Sample_contact_zip/postal_code	59202-9037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659714/suppl/GSM659714.CEL.gz
Sample_series_id	GSE26828
Sample_data_row_count	26877

GSM659715

GPL570

UROtsa_Cd_6

UROtsa cell, Cadmium-transformed

cell line: UROtsa agent: Cadmium

Gene expression data from cadmium-transformed UROtsa cells

Sample_geo_accession	GSM659715
Sample_status	Public on Jan 25 2011
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	1 micromolar cadmium as cadmium chloride was added to the growth medium and the cells were passaged weekly until the cells attained the ability to form colonies in soft agar.
Sample_growth_protocol_ch1	Cells grown in Dulbeco's modified Eagles medium supplemented with 5% FCS. Split ratio was 1-4 weekly.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested in normal medium without cadmium and the RNA purified using the Rneasy Kit from Qiagen.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was biotinylated using the Bioarray cDNA Synthesis kit (ENZO Diagnostics, Farmingdale, NY)
Sample_hyb_protocol	15 ug of fragmented labeled RNA was hybridized for 16 hr at 45 degree C to an Affymetrix U133 Plus 2.0 array. Arrays were wased and stained using a Fluidics Station 450 according to the manufacturer's instructions. Arrays were stained with phycoerythrein-conjugated streptavidin.
Sample_scan_protocol	The fluorescence intensities were determined using a GCS 3000 7G high-resolution confocal laser scanner from Affymetrix.
Sample_data_processing	Images were analyzed using programs resident in GeneChip Operating System v1.4 (GCOS; Affymetrix). Quality control metrics for cRNA integrity, sample loading, and variations in staining were determined after background correction and signal summarization by MAS 5.0. The probes whose MAS5 Detection Call=Absent for all 9 samples were filtered out prior to statistical analysis, and thus were not included in the data table.
Sample_platform_id	GPL570
Sample_contact_name	Donald,,Sens
Sample_contact_email	donald.sens@med.und.edu
Sample_contact_phone	701-777-6258
Sample_contact_fax	701-777-3108
Sample_contact_department	Pathology
Sample_contact_institute	University of North Dakota
Sample_contact_address	501 North Columbia Road
Sample_contact_city	Grand Forks
Sample_contact_state	ND
Sample_contact_zip/postal_code	59202-9037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659715/suppl/GSM659715.CEL.gz
Sample_series_id	GSE26828
Sample_data_row_count	26877

GSM659716

GPL570

UROtsa_Cd_7

UROtsa cell, Cadmium-transformed

cell line: UROtsa agent: Cadmium

Gene expression data from cadmium-transformed UROtsa cells

Sample_geo_accession	GSM659716
Sample_status	Public on Jan 25 2011
Sample_submission_date	Jan 24 2011
Sample_last_update_date	Jan 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	1 micromolar cadmium as cadmium chloride was added to the growth medium and the cells were passaged weekly until the cells attained the ability to form colonies in soft agar.
Sample_growth_protocol_ch1	Cells grown in Dulbeco's modified Eagles medium supplemented with 5% FCS. Split ratio was 1-4 weekly.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Cells were harvested in normal medium without cadmium and the RNA purified using the Rneasy Kit from Qiagen.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA was biotinylated using the Bioarray cDNA Synthesis kit (ENZO Diagnostics, Farmingdale, NY)
Sample_hyb_protocol	15 ug of fragmented labeled RNA was hybridized for 16 hr at 45 degree C to an Affymetrix U133 Plus 2.0 array. Arrays were wased and stained using a Fluidics Station 450 according to the manufacturer's instructions. Arrays were stained with phycoerythrein-conjugated streptavidin.
Sample_scan_protocol	The fluorescence intensities were determined using a GCS 3000 7G high-resolution confocal laser scanner from Affymetrix.
Sample_data_processing	Images were analyzed using programs resident in GeneChip Operating System v1.4 (GCOS; Affymetrix). Quality control metrics for cRNA integrity, sample loading, and variations in staining were determined after background correction and signal summarization by MAS 5.0. The probes whose MAS5 Detection Call=Absent for all 9 samples were filtered out prior to statistical analysis, and thus were not included in the data table.
Sample_platform_id	GPL570
Sample_contact_name	Donald,,Sens
Sample_contact_email	donald.sens@med.und.edu
Sample_contact_phone	701-777-6258
Sample_contact_fax	701-777-3108
Sample_contact_department	Pathology
Sample_contact_institute	University of North Dakota
Sample_contact_address	501 North Columbia Road
Sample_contact_city	Grand Forks
Sample_contact_state	ND
Sample_contact_zip/postal_code	59202-9037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM659nnn/GSM659716/suppl/GSM659716.CEL.gz
Sample_series_id	GSE26828
Sample_data_row_count	26877

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