Search results for the GEO ID: GSE26869  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM661472 | GPL96 |
|
skeletal muscle main population cells from fetal human subject 2
|
skeletal muscle main population cells from fetal human subject 2
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 18 weeks
percentage (%) of sp cells: 1.07
percentage (%) of mp cells: 24.00
microarray hybridization date: 09/26/2002
microarray data quality: good
|
skeletal muscle main population cells from fetal human subject 2
|
| Sample_geo_accession | GSM661472
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661472/suppl/GSM661472.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661473 | GPL96 |
|
skeletal muscle main population cells from fetal human subject 3
|
skeletal muscle main population cells from fetal human subject 3
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 14 weeks
percentage (%) of sp cells: 1.02
percentage (%) of mp cells: 76.00
microarray hybridization date: 10/03/2002
microarray data quality: good
|
skeletal muscle main population cells from fetal human subject 3
|
| Sample_geo_accession | GSM661473
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661473/suppl/GSM661473.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661474 | GPL96 |
|
skeletal muscle main population cells from fetal human subject 4
|
skeletal muscle main population cells from fetal human subject 4
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 17 weeks
percentage (%) of sp cells: 1.50
percentage (%) of mp cells: 80.30
microarray hybridization date: 10/10/2002
microarray data quality: good
|
skeletal muscle main population cells from fetal human subject 4
|
| Sample_geo_accession | GSM661474
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661474/suppl/GSM661474.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661475 | GPL96 |
|
skeletal muscle main population cells from fetal human subject 5
|
skeletal muscle main population cells from fetal human subject 5
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 14 weeks
percentage (%) of sp cells: 3.43
percentage (%) of mp cells: 8.54
microarray hybridization date: 11/14/2002
microarray data quality: good
|
skeletal muscle main population cells from fetal human subject 5
|
| Sample_geo_accession | GSM661475
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661475/suppl/GSM661475.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661476 | GPL96 |
|
skeletal muscle main population cells from fetal human subject 7
|
skeletal muscle main population cells from fetal human subject 7
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 14 weeks
percentage (%) of sp cells: 3.22
percentage (%) of mp cells: 21.30
microarray hybridization date: 11/18/2002
microarray data quality: good
|
skeletal muscle main population cells from fetal human subject 7
|
| Sample_geo_accession | GSM661476
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661476/suppl/GSM661476.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661477 | GPL96 |
|
skeletal muscle main population cells from fetal human subject 9
|
skeletal muscle main population cells from fetal human subject 9
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 14 weeks
percentage (%) of sp cells: 2.05
percentage (%) of mp cells: 63.40
microarray hybridization date: 12/02/2002
microarray data quality: good
|
skeletal muscle main population cells from fetal human subject 9
|
| Sample_geo_accession | GSM661477
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661477/suppl/GSM661477.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661478 | GPL96 |
|
skeletal muscle main population cells from fetal human subject 12
|
skeletal muscle main population cells from fetal human subject 12
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 18 weeks
percentage (%) of sp cells: 1.57
percentage (%) of mp cells: 45.43
microarray hybridization date: 12/09/2002
microarray data quality: good
|
skeletal muscle main population cells from fetal human subject 12
|
| Sample_geo_accession | GSM661478
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661478/suppl/GSM661478.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661479 | GPL96 |
|
skeletal muscle main population cells from fetal human subject 13
|
skeletal muscle main population cells from fetal human subject 13
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 15 weeks
percentage (%) of sp cells: 6.57
percentage (%) of mp cells: 51.33
microarray hybridization date: 12/16/2002
microarray data quality: good
|
skeletal muscle main population cells from fetal human subject 13
|
| Sample_geo_accession | GSM661479
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661479/suppl/GSM661479.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661480 | GPL96 |
|
skeletal muscle main population cells from fetal human subject 14
|
skeletal muscle main population cells from fetal human subject 14
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 14 weeks
percentage (%) of sp cells: 0.00
percentage (%) of mp cells: 67.73
microarray hybridization date: 12/16/2002
microarray data quality: good
|
skeletal muscle main population cells from fetal human subject 14
|
| Sample_geo_accession | GSM661480
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661480/suppl/GSM661480.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661481 | GPL96 |
|
skeletal muscle main population cells from fetal human subject 15
|
skeletal muscle main population cells from fetal human subject 15
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 18 weeks
percentage (%) of sp cells: 1.50
percentage (%) of mp cells: 48.70
microarray hybridization date: 12/16/2002
microarray data quality: good
|
skeletal muscle main population cells from fetal human subject 15
|
| Sample_geo_accession | GSM661481
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661481/suppl/GSM661481.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661482 | GPL96 |
|
skeletal muscle side population cells from fetal human subject 1
|
skeletal muscle side population cells from fetal human subject 1
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 18 weeks
percentage (%) of sp cells: 4.27
percentage (%) of mp cells: 36.80
microarray hybridization date: 09/11/2002
microarray data quality: good
|
skeletal muscle side population cells from fetal human subject 1
|
| Sample_geo_accession | GSM661482
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661482/suppl/GSM661482.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661483 | GPL96 |
|
skeletal muscle side population cells from fetal human subject 2
|
skeletal muscle side population cells from fetal human subject 2
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 18 weeks
percentage (%) of sp cells: 1.07
percentage (%) of mp cells: 24.00
microarray hybridization date: 09/26/2002
microarray data quality: good
|
skeletal muscle side population cells from fetal human subject 2
|
| Sample_geo_accession | GSM661483
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661483/suppl/GSM661483.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661484 | GPL96 |
|
skeletal muscle side population cells from fetal human subject 3
|
skeletal muscle side population cells from fetal human subject 3
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 14 weeks
percentage (%) of sp cells: 1.02
percentage (%) of mp cells: 76.00
microarray hybridization date: 10/03/2002
microarray data quality: poor
|
skeletal muscle side population cells from fetal human subject 3
|
| Sample_geo_accession | GSM661484
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661484/suppl/GSM661484.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661485 | GPL96 |
|
skeletal muscle side population cells from fetal human subject 4
|
skeletal muscle side population cells from fetal human subject 4
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 17 weeks
percentage (%) of sp cells: 1.50
percentage (%) of mp cells: 80.30
microarray hybridization date: 10/10/2002
microarray data quality: good
|
skeletal muscle side population cells from fetal human subject 4
|
| Sample_geo_accession | GSM661485
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661485/suppl/GSM661485.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661486 | GPL96 |
|
skeletal muscle side population cells from fetal human subject 5
|
skeletal muscle side population cells from fetal human subject 5
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 14 weeks
percentage (%) of sp cells: 3.43
percentage (%) of mp cells: 8.54
microarray hybridization date: 11/14/2002
microarray data quality: good
|
skeletal muscle side population cells from fetal human subject 5
|
| Sample_geo_accession | GSM661486
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661486/suppl/GSM661486.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661487 | GPL96 |
|
skeletal muscle side population cells from fetal human subject 6
|
skeletal muscle side population cells from fetal human subject 6
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 14 weeks
percentage (%) of sp cells: 2.61
percentage (%) of mp cells: 10.87
microarray hybridization date: 11/14/2002
microarray data quality: good
|
skeletal muscle side population cells from fetal human subject 6
|
| Sample_geo_accession | GSM661487
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661487/suppl/GSM661487.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661488 | GPL96 |
|
skeletal muscle side population cells from fetal human subject 7
|
skeletal muscle side population cells from fetal human subject 7
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 14 weeks
percentage (%) of sp cells: 3.22
percentage (%) of mp cells: 21.30
microarray hybridization date: 11/18/2002
microarray data quality: good
|
skeletal muscle side population cells from fetal human subject 7
|
| Sample_geo_accession | GSM661488
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661488/suppl/GSM661488.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661489 | GPL96 |
|
skeletal muscle side population cells from fetal human subject 8
|
skeletal muscle side population cells from fetal human subject 8
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 17 weeks
percentage (%) of sp cells: -
percentage (%) of mp cells: -
microarray hybridization date: 11/18/2002
microarray data quality: poor
|
skeletal muscle side population cells from fetal human subject 8
|
| Sample_geo_accession | GSM661489
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661489/suppl/GSM661489.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661490 | GPL96 |
|
skeletal muscle side population cells from fetal human subject 9
|
skeletal muscle side population cells from fetal human subject 9
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 14 weeks
percentage (%) of sp cells: 2.05
percentage (%) of mp cells: 63.40
microarray hybridization date: 12/02/2002
microarray data quality: good
|
skeletal muscle side population cells from fetal human subject 9
|
| Sample_geo_accession | GSM661490
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661490/suppl/GSM661490.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661491 | GPL96 |
|
skeletal muscle side population cells from fetal human subject 10
|
skeletal muscle side population cells from fetal human subject 10
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 17 weeks
percentage (%) of sp cells: 4.13
percentage (%) of mp cells: 8.54
microarray hybridization date: 12/02/2002
microarray data quality: good
|
skeletal muscle side population cells from fetal human subject 10
|
| Sample_geo_accession | GSM661491
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661491/suppl/GSM661491.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661492 | GPL96 |
|
skeletal muscle side population cells from fetal human subject 11
|
skeletal muscle side population cells from fetal human subject 11
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 17 weeks
percentage (%) of sp cells: 2.38
percentage (%) of mp cells: 11.74
microarray hybridization date: 12/09/2002
microarray data quality: good
|
skeletal muscle side population cells from fetal human subject 11
|
| Sample_geo_accession | GSM661492
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661492/suppl/GSM661492.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
|
GSM661493 | GPL96 |
|
skeletal muscle side population cells from fetal human subject 13
|
skeletal muscle side population cells from fetal human subject 13
|
tissue of origin: skeletal muscle
gestational age of subject in weeks: 15 weeks
percentage (%) of sp cells: 6.57
percentage (%) of mp cells: 51.33
microarray hybridization date: 12/16/2002
microarray data quality: good
|
skeletal muscle side population cells from fetal human subject 13
|
| Sample_geo_accession | GSM661493
| | Sample_status | Public on Jan 26 2011
| | Sample_submission_date | Jan 26 2011
| | Sample_last_update_date | Jan 26 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
| | Sample_hyb_protocol | Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
| | Sample_scan_protocol | Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
| | Sample_data_processing | CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Alvin,T.,Kho
| | Sample_contact_email | alvin_kho@hms.harvard.edu
| | Sample_contact_phone | 617-355-6393
| | Sample_contact_department | Informatics Program
| | Sample_contact_institute | Boston Children's Hospital
| | Sample_contact_address | 320 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM661nnn/GSM661493/suppl/GSM661493.CEL.gz
| | Sample_series_id | GSE26869
| | Sample_data_row_count | 22283
| | |
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