Result

Search results for the GEO ID: GSE26912

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Title

Source name

Description

Characteristics

GSM662788

GPL1261

Tumoricidal macrophages, biological rep1

Mouse macrophages, tumoricidal

cell type: CD11b+ macrophages cell phenotype: tumoricidal genetic background: Balb/c age: adult (7-12 weeks) cell source: Idiotype-specific TCR-transgenic SCID mice injected with MOPC315-containing Matrigel

Tumoricidal macrophages were isolated from Matrigel containing MOPC315 in Idiotype-specific-TCR-transgenic mice at day +8.

Sample_geo_accession	GSM662788
Sample_status	Public on Mar 15 2011
Sample_submission_date	Jan 27 2011
Sample_last_update_date	Mar 15 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Idiotype-specific TCR-transgenic SCID Balb/c mice were injected s.c. with MOPC315 (or antigen-loss MOPC315) myeloma cells in Matrigel. CD11b+ macrophages were isolated by FACSAria sorting.
Sample_growth_protocol_ch1	Macrophages that had infiltrated subcutaneous Matrigel plugs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
Sample_data_processing	The data were analyzed with GeneChip Operating Software 1.3 (Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Alexandre,,Corthay
Sample_contact_email	alexandre.corthay@medisin.uio.no
Sample_contact_laboratory	Tumor Immunology Research Group
Sample_contact_department	Department of Biosciences
Sample_contact_institute	University of Oslo
Sample_contact_address	Rikshospitalet
Sample_contact_city	Oslo
Sample_contact_zip/postal_code	P.O. Box 4950 Nydalen, 0424
Sample_contact_country	Norway
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662788/suppl/GSM662788_Tumoricidal_macrophages_1.CEL.gz
Sample_series_id	GSE26912
Sample_data_row_count	45101

GSM662789

GPL1261

Tumoricidal macrophages, biological rep2

Mouse macrophages, tumoricidal

Tumoricidal macrophages were isolated from Matrigel containing MOPC315 in Idiotype-specific-TCR-transgenic mice at day +8.

Sample_geo_accession	GSM662789
Sample_status	Public on Mar 15 2011
Sample_submission_date	Jan 27 2011
Sample_last_update_date	Mar 15 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Idiotype-specific TCR-transgenic SCID Balb/c mice were injected s.c. with MOPC315 (or antigen-loss MOPC315) myeloma cells in Matrigel. CD11b+ macrophages were isolated by FACSAria sorting.
Sample_growth_protocol_ch1	Macrophages that had infiltrated subcutaneous Matrigel plugs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
Sample_data_processing	The data were analyzed with GeneChip Operating Software 1.3 (Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Alexandre,,Corthay
Sample_contact_email	alexandre.corthay@medisin.uio.no
Sample_contact_laboratory	Tumor Immunology Research Group
Sample_contact_department	Department of Biosciences
Sample_contact_institute	University of Oslo
Sample_contact_address	Rikshospitalet
Sample_contact_city	Oslo
Sample_contact_zip/postal_code	P.O. Box 4950 Nydalen, 0424
Sample_contact_country	Norway
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662789/suppl/GSM662789_Tumoricidal_macrophages_2.CEL.gz
Sample_series_id	GSE26912
Sample_data_row_count	45101

GSM662790

GPL1261

Tumoricidal macrophages, biological rep3

Mouse macrophages, tumoricidal

Tumoricidal macrophages were isolated from Matrigel containing MOPC315 in Idiotype-specific-TCR-transgenic mice at day +8.

Sample_geo_accession	GSM662790
Sample_status	Public on Mar 15 2011
Sample_submission_date	Jan 27 2011
Sample_last_update_date	Mar 15 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Idiotype-specific TCR-transgenic SCID Balb/c mice were injected s.c. with MOPC315 (or antigen-loss MOPC315) myeloma cells in Matrigel. CD11b+ macrophages were isolated by FACSAria sorting.
Sample_growth_protocol_ch1	Macrophages that had infiltrated subcutaneous Matrigel plugs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
Sample_data_processing	The data were analyzed with GeneChip Operating Software 1.3 (Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Alexandre,,Corthay
Sample_contact_email	alexandre.corthay@medisin.uio.no
Sample_contact_laboratory	Tumor Immunology Research Group
Sample_contact_department	Department of Biosciences
Sample_contact_institute	University of Oslo
Sample_contact_address	Rikshospitalet
Sample_contact_city	Oslo
Sample_contact_zip/postal_code	P.O. Box 4950 Nydalen, 0424
Sample_contact_country	Norway
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662790/suppl/GSM662790_Tumoricidal_macrophages_3.CEL.gz
Sample_series_id	GSE26912
Sample_data_row_count	45101

GSM662791

GPL1261

Control macrophages, biological rep1

Mouse macrophages, non-tumoricidal

cell type: CD11b+ macrophages cell phenotype: non-tumoricidal genetic background: Balb/c age: adult (7-12 weeks) cell source: TCR-transgenic SCID mice injected with Matrigel containing antigen-loss MOPC315

Control macrophages were isolated from Matrigel containing antigen-loss MOPC315 in Idiotype-specific-TCR-transgenic mice at day +8.

Sample_geo_accession	GSM662791
Sample_status	Public on Mar 15 2011
Sample_submission_date	Jan 27 2011
Sample_last_update_date	Mar 15 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Idiotype-specific TCR-transgenic SCID Balb/c mice were injected s.c. with MOPC315 (or antigen-loss MOPC315) myeloma cells in Matrigel. CD11b+ macrophages were isolated by FACSAria sorting.
Sample_growth_protocol_ch1	Macrophages that had infiltrated subcutaneous Matrigel plugs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
Sample_data_processing	The data were analyzed with GeneChip Operating Software 1.3 (Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Alexandre,,Corthay
Sample_contact_email	alexandre.corthay@medisin.uio.no
Sample_contact_laboratory	Tumor Immunology Research Group
Sample_contact_department	Department of Biosciences
Sample_contact_institute	University of Oslo
Sample_contact_address	Rikshospitalet
Sample_contact_city	Oslo
Sample_contact_zip/postal_code	P.O. Box 4950 Nydalen, 0424
Sample_contact_country	Norway
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662791/suppl/GSM662791_Control_macrophages_1.CEL.gz
Sample_series_id	GSE26912
Sample_data_row_count	45101

GSM662792

GPL1261

Control macrophages, biological rep2

Mouse macrophages, non-tumoricidal

Control macrophages were isolated from Matrigel containing antigen-loss MOPC315 in Idiotype-specific-TCR-transgenic mice at day +8.

Sample_geo_accession	GSM662792
Sample_status	Public on Mar 15 2011
Sample_submission_date	Jan 27 2011
Sample_last_update_date	Mar 15 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Idiotype-specific TCR-transgenic SCID Balb/c mice were injected s.c. with MOPC315 (or antigen-loss MOPC315) myeloma cells in Matrigel. CD11b+ macrophages were isolated by FACSAria sorting.
Sample_growth_protocol_ch1	Macrophages that had infiltrated subcutaneous Matrigel plugs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
Sample_data_processing	The data were analyzed with GeneChip Operating Software 1.3 (Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Alexandre,,Corthay
Sample_contact_email	alexandre.corthay@medisin.uio.no
Sample_contact_laboratory	Tumor Immunology Research Group
Sample_contact_department	Department of Biosciences
Sample_contact_institute	University of Oslo
Sample_contact_address	Rikshospitalet
Sample_contact_city	Oslo
Sample_contact_zip/postal_code	P.O. Box 4950 Nydalen, 0424
Sample_contact_country	Norway
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662792/suppl/GSM662792_Control_macrophages_2.CEL.gz
Sample_series_id	GSE26912
Sample_data_row_count	45101

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