Result

Search results for the GEO ID: GSE26921

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GSM ID

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Title

Source name

Description

Characteristics

GSM662887

GPL570

NCI-H929 DMSO Control

B lymphocyte

cell type: Multiple Myeloma Cell Line cell line: NCI-H929 treatment group: DMSO Control

1.H929 DMSO NCI-H929-DMSO

Sample_geo_accession	GSM662887
Sample_status	Public on Jul 04 2011
Sample_submission_date	Jan 27 2011
Sample_last_update_date	Jul 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with 0.5 μmol/L DZNep for 48 h.
Sample_growth_protocol_ch1	Human MM cell lines KMS18, MM.1S and OPM-2 were maintained in RPMI 1640, supplemented with 10% fetal calf serum (FCS), 100 U/mL penicillin and 100 ug/mL streptomycin. MM cell line NCI-H929 was cultured in RPMI 1640 with 15% FCS and 0.00036% 2-mercaptoethanol. All cells were grown at 37°C in a humidified atmosphere with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNeasy Mini kit (Germany) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChip 3' IVT Express Kit Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix scanner 3000 7G
Sample_data_processing	The data were analyzed with Genespring GX (Agilent Technologies). The data normalization was done using MAS5 algorithm in GeneSpring GX. For each control-treatment comparison, entities that have flagged present or marginal in at least 1 out of 2 samples were retained, and the differentially expressed genes were identified using the fold change function.
Sample_platform_id	GPL570
Sample_contact_name	Zhigang,,Xie
Sample_contact_email	xiezhigang@nus.edu.sg
Sample_contact_institute	National University of Singapore
Sample_contact_address	28 Medical Drive, CeLS Building #02-07
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117456
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662887/suppl/GSM662887.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662887/suppl/GSM662887.CHP.gz
Sample_series_id	GSE26921
Sample_data_row_count	54675

GSM662888

GPL570

NCI-H929 DZNep treated

B lymphocyte

cell type: Multiple Myeloma Cell Line cell line: NCI-H929 treatment group: DZNep treated

2.H929 DZNep NCI-H929-DZNep

Sample_geo_accession	GSM662888
Sample_status	Public on Jul 04 2011
Sample_submission_date	Jan 27 2011
Sample_last_update_date	Jul 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with 0.5 μmol/L DZNep for 48 h.
Sample_growth_protocol_ch1	Human MM cell lines KMS18, MM.1S and OPM-2 were maintained in RPMI 1640, supplemented with 10% fetal calf serum (FCS), 100 U/mL penicillin and 100 ug/mL streptomycin. MM cell line NCI-H929 was cultured in RPMI 1640 with 15% FCS and 0.00036% 2-mercaptoethanol. All cells were grown at 37°C in a humidified atmosphere with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNeasy Mini kit (Germany) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChip 3' IVT Express Kit Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix scanner 3000 7G
Sample_data_processing	The data were analyzed with Genespring GX (Agilent Technologies). The data normalization was done using MAS5 algorithm in GeneSpring GX. For each control-treatment comparison, entities that have flagged present or marginal in at least 1 out of 2 samples were retained, and the differentially expressed genes were identified using the fold change function.
Sample_platform_id	GPL570
Sample_contact_name	Zhigang,,Xie
Sample_contact_email	xiezhigang@nus.edu.sg
Sample_contact_institute	National University of Singapore
Sample_contact_address	28 Medical Drive, CeLS Building #02-07
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117456
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662888/suppl/GSM662888.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662888/suppl/GSM662888.CHP.gz
Sample_series_id	GSE26921
Sample_data_row_count	54675

GSM662889

GPL570

MM1.S DMSO Control

B lymphocyte

cell type: Multiple Myeloma Cell Line cell line: MM.1S treatment group: DMSO Control

3.MM1S DMSO MM1.S-DMSO

Sample_geo_accession	GSM662889
Sample_status	Public on Jul 04 2011
Sample_submission_date	Jan 27 2011
Sample_last_update_date	Jul 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with 0.5 μmol/L DZNep for 48 h.
Sample_growth_protocol_ch1	Human MM cell lines KMS18, MM.1S and OPM-2 were maintained in RPMI 1640, supplemented with 10% fetal calf serum (FCS), 100 U/mL penicillin and 100 ug/mL streptomycin. MM cell line NCI-H929 was cultured in RPMI 1640 with 15% FCS and 0.00036% 2-mercaptoethanol. All cells were grown at 37°C in a humidified atmosphere with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNeasy Mini kit (Germany) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChip 3' IVT Express Kit Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix scanner 3000 7G
Sample_data_processing	The data were analyzed with Genespring GX (Agilent Technologies). The data normalization was done using MAS5 algorithm in GeneSpring GX. For each control-treatment comparison, entities that have flagged present or marginal in at least 1 out of 2 samples were retained, and the differentially expressed genes were identified using the fold change function.
Sample_platform_id	GPL570
Sample_contact_name	Zhigang,,Xie
Sample_contact_email	xiezhigang@nus.edu.sg
Sample_contact_institute	National University of Singapore
Sample_contact_address	28 Medical Drive, CeLS Building #02-07
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117456
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662889/suppl/GSM662889.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662889/suppl/GSM662889.CHP.gz
Sample_series_id	GSE26921
Sample_data_row_count	54675

GSM662890

GPL570

MM1.S DZNep treated

B lymphocyte

cell type: Multiple Myeloma Cell Line cell line: MM.1S treatment group: DZNep treated

4.MM1S DZNep MM1.S-DZNep

Sample_geo_accession	GSM662890
Sample_status	Public on Jul 04 2011
Sample_submission_date	Jan 27 2011
Sample_last_update_date	Jul 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with 0.5 μmol/L DZNep for 48 h.
Sample_growth_protocol_ch1	Human MM cell lines KMS18, MM.1S and OPM-2 were maintained in RPMI 1640, supplemented with 10% fetal calf serum (FCS), 100 U/mL penicillin and 100 ug/mL streptomycin. MM cell line NCI-H929 was cultured in RPMI 1640 with 15% FCS and 0.00036% 2-mercaptoethanol. All cells were grown at 37°C in a humidified atmosphere with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNeasy Mini kit (Germany) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChip 3' IVT Express Kit Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix scanner 3000 7G
Sample_data_processing	The data were analyzed with Genespring GX (Agilent Technologies). The data normalization was done using MAS5 algorithm in GeneSpring GX. For each control-treatment comparison, entities that have flagged present or marginal in at least 1 out of 2 samples were retained, and the differentially expressed genes were identified using the fold change function.
Sample_platform_id	GPL570
Sample_contact_name	Zhigang,,Xie
Sample_contact_email	xiezhigang@nus.edu.sg
Sample_contact_institute	National University of Singapore
Sample_contact_address	28 Medical Drive, CeLS Building #02-07
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117456
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662890/suppl/GSM662890.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662890/suppl/GSM662890.CHP.gz
Sample_series_id	GSE26921
Sample_data_row_count	54675

GSM662891

GPL570

KMS18 DMSO Control

B lymphocyte

cell type: Multiple Myeloma Cell Line cell line: KMS18 treatment group: DMSO Control

5.KMS18 DMSO KMS18-DMSO

Sample_geo_accession	GSM662891
Sample_status	Public on Jul 04 2011
Sample_submission_date	Jan 27 2011
Sample_last_update_date	Jul 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with 0.5 μmol/L DZNep for 48 h.
Sample_growth_protocol_ch1	Human MM cell lines KMS18, MM.1S and OPM-2 were maintained in RPMI 1640, supplemented with 10% fetal calf serum (FCS), 100 U/mL penicillin and 100 ug/mL streptomycin. MM cell line NCI-H929 was cultured in RPMI 1640 with 15% FCS and 0.00036% 2-mercaptoethanol. All cells were grown at 37°C in a humidified atmosphere with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNeasy Mini kit (Germany) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChip 3' IVT Express Kit Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix scanner 3000 7G
Sample_data_processing	The data were analyzed with Genespring GX (Agilent Technologies). The data normalization was done using MAS5 algorithm in GeneSpring GX. For each control-treatment comparison, entities that have flagged present or marginal in at least 1 out of 2 samples were retained, and the differentially expressed genes were identified using the fold change function.
Sample_platform_id	GPL570
Sample_contact_name	Zhigang,,Xie
Sample_contact_email	xiezhigang@nus.edu.sg
Sample_contact_institute	National University of Singapore
Sample_contact_address	28 Medical Drive, CeLS Building #02-07
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117456
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662891/suppl/GSM662891.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662891/suppl/GSM662891.CHP.gz
Sample_series_id	GSE26921
Sample_data_row_count	54675

GSM662892

GPL570

KMS18 DZNep treated

B lymphocyte

cell type: Multiple Myeloma Cell Line cell line: KMS18 treatment group: DZNep treated

6.KMS18 DZNep KMS18-DZNep

Sample_geo_accession	GSM662892
Sample_status	Public on Jul 04 2011
Sample_submission_date	Jan 27 2011
Sample_last_update_date	Jul 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with 0.5 μmol/L DZNep for 48 h.
Sample_growth_protocol_ch1	Human MM cell lines KMS18, MM.1S and OPM-2 were maintained in RPMI 1640, supplemented with 10% fetal calf serum (FCS), 100 U/mL penicillin and 100 ug/mL streptomycin. MM cell line NCI-H929 was cultured in RPMI 1640 with 15% FCS and 0.00036% 2-mercaptoethanol. All cells were grown at 37°C in a humidified atmosphere with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNeasy Mini kit (Germany) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChip 3' IVT Express Kit Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix scanner 3000 7G
Sample_data_processing	The data were analyzed with Genespring GX (Agilent Technologies). The data normalization was done using MAS5 algorithm in GeneSpring GX. For each control-treatment comparison, entities that have flagged present or marginal in at least 1 out of 2 samples were retained, and the differentially expressed genes were identified using the fold change function.
Sample_platform_id	GPL570
Sample_contact_name	Zhigang,,Xie
Sample_contact_email	xiezhigang@nus.edu.sg
Sample_contact_institute	National University of Singapore
Sample_contact_address	28 Medical Drive, CeLS Building #02-07
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117456
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662892/suppl/GSM662892.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662892/suppl/GSM662892.CHP.gz
Sample_series_id	GSE26921
Sample_data_row_count	54675

GSM662893

GPL570

OPM2 DMSO Control

B lymphocyte

cell type: Multiple Myeloma Cell Line cell line: OPM-2 treatment group: DMSO Control

7.OPM-2 DMSO OPM2-DMSO

Sample_geo_accession	GSM662893
Sample_status	Public on Jul 04 2011
Sample_submission_date	Jan 27 2011
Sample_last_update_date	Jul 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with 0.5 μmol/L DZNep for 48 h.
Sample_growth_protocol_ch1	Human MM cell lines KMS18, MM.1S and OPM-2 were maintained in RPMI 1640, supplemented with 10% fetal calf serum (FCS), 100 U/mL penicillin and 100 ug/mL streptomycin. MM cell line NCI-H929 was cultured in RPMI 1640 with 15% FCS and 0.00036% 2-mercaptoethanol. All cells were grown at 37°C in a humidified atmosphere with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNeasy Mini kit (Germany) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChip 3' IVT Express Kit Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix scanner 3000 7G
Sample_data_processing	The data were analyzed with Genespring GX (Agilent Technologies). The data normalization was done using MAS5 algorithm in GeneSpring GX. For each control-treatment comparison, entities that have flagged present or marginal in at least 1 out of 2 samples were retained, and the differentially expressed genes were identified using the fold change function.
Sample_platform_id	GPL570
Sample_contact_name	Zhigang,,Xie
Sample_contact_email	xiezhigang@nus.edu.sg
Sample_contact_institute	National University of Singapore
Sample_contact_address	28 Medical Drive, CeLS Building #02-07
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117456
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662893/suppl/GSM662893.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662893/suppl/GSM662893.CHP.gz
Sample_series_id	GSE26921
Sample_data_row_count	54675

GSM662894

GPL570

OPM2 DZNep treated

B lymphocyte

cell type: Multiple Myeloma Cell Line cell line: OPM-2 treatment group: DZNep treated

8.OPM-2 DZNep OPM2-DZNep

Sample_geo_accession	GSM662894
Sample_status	Public on Jul 04 2011
Sample_submission_date	Jan 27 2011
Sample_last_update_date	Jul 04 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were treated with 0.5 μmol/L DZNep for 48 h.
Sample_growth_protocol_ch1	Human MM cell lines KMS18, MM.1S and OPM-2 were maintained in RPMI 1640, supplemented with 10% fetal calf serum (FCS), 100 U/mL penicillin and 100 ug/mL streptomycin. MM cell line NCI-H929 was cultured in RPMI 1640 with 15% FCS and 0.00036% 2-mercaptoethanol. All cells were grown at 37°C in a humidified atmosphere with 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNeasy Mini kit (Germany) extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChip 3' IVT Express Kit Manual, 2009, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix scanner 3000 7G
Sample_data_processing	The data were analyzed with Genespring GX (Agilent Technologies). The data normalization was done using MAS5 algorithm in GeneSpring GX. For each control-treatment comparison, entities that have flagged present or marginal in at least 1 out of 2 samples were retained, and the differentially expressed genes were identified using the fold change function.
Sample_platform_id	GPL570
Sample_contact_name	Zhigang,,Xie
Sample_contact_email	xiezhigang@nus.edu.sg
Sample_contact_institute	National University of Singapore
Sample_contact_address	28 Medical Drive, CeLS Building #02-07
Sample_contact_city	Singapore
Sample_contact_zip/postal_code	117456
Sample_contact_country	Singapore
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662894/suppl/GSM662894.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM662nnn/GSM662894/suppl/GSM662894.CHP.gz
Sample_series_id	GSE26921
Sample_data_row_count	54675

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