Search results for the GEO ID: GSE27049  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM667580 | GPL1261 |
|
control siRNA_rep1
|
MII eggs injected with control siRNA
|
strain: CF1
condition: control siRNA
developmental stage: MII egg
|
Gene expression data from in vitro matured mouse MII eggs injected with control siRNA
|
| Sample_geo_accession | GSM667580
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Feb 03 2011
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Full-grown GV oocytes were injected with Dcp1a siRNA (Dharmacon, On-TARGETplus SMARTpool, L-065144-01) or Dcp2 siRNA (Dharmacon, On-TARGETplus SMARTpool L-040353-01). Dcp1a&Dcp2 double knockdown were achieved by injecting a mixture of the above siRNAs.
| | Sample_growth_protocol_ch1 | Microinjected oocytes were cultured in CZB with milrinone for 20 h and then transferred to milrinone-free medium and allowed to mature to MII for 18 h. The MII eggs were then used for RNA extraction and microarray analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from pools of 30 MII eggs per samples using Pico-Pure RNA Isolation kit (Acturus).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA samples were amplified and labelled according to Affymetrix protocol GeneChip Eukaryotic Small Sample Target Labelling Assay Version II.
| | Sample_hyb_protocol | Standard Affymetrix procedures
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | The data were loaded into R software and normalized using AffyPLM package of the Bioconductor software. GCRMA algorithm was used for normalization. The values lower than 10.0 represent detection absent (with detection call P-value P>0.05).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM667nnn/GSM667580/suppl/GSM667580.CEL.gz
| | Sample_series_id | GSE27049
| | Sample_data_row_count | 45101
| | |
|
GSM667581 | GPL1261 |
|
control siRNA_rep2
|
MII eggs injected with control siRNA
|
strain: CF1
condition: control siRNA
developmental stage: MII egg
|
Gene expression data from in vitro matured mouse MII eggs injected with control siRNA
|
| Sample_geo_accession | GSM667581
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Feb 03 2011
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Full-grown GV oocytes were injected with Dcp1a siRNA (Dharmacon, On-TARGETplus SMARTpool, L-065144-01) or Dcp2 siRNA (Dharmacon, On-TARGETplus SMARTpool L-040353-01). Dcp1a&Dcp2 double knockdown were achieved by injecting a mixture of the above siRNAs.
| | Sample_growth_protocol_ch1 | Microinjected oocytes were cultured in CZB with milrinone for 20 h and then transferred to milrinone-free medium and allowed to mature to MII for 18 h. The MII eggs were then used for RNA extraction and microarray analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from pools of 30 MII eggs per samples using Pico-Pure RNA Isolation kit (Acturus).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA samples were amplified and labelled according to Affymetrix protocol GeneChip Eukaryotic Small Sample Target Labelling Assay Version II.
| | Sample_hyb_protocol | Standard Affymetrix procedures
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | The data were loaded into R software and normalized using AffyPLM package of the Bioconductor software. GCRMA algorithm was used for normalization. The values lower than 10.0 represent detection absent (with detection call P-value P>0.05).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM667nnn/GSM667581/suppl/GSM667581.CEL.gz
| | Sample_series_id | GSE27049
| | Sample_data_row_count | 45101
| | |
|
GSM667582 | GPL1261 |
|
control siRNA_rep3
|
MII eggs injected with control siRNA
|
strain: CF1
condition: control siRNA
developmental stage: MII egg
|
Gene expression data from in vitro matured mouse MII eggs injected with control siRNA
|
| Sample_geo_accession | GSM667582
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Feb 03 2011
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Full-grown GV oocytes were injected with Dcp1a siRNA (Dharmacon, On-TARGETplus SMARTpool, L-065144-01) or Dcp2 siRNA (Dharmacon, On-TARGETplus SMARTpool L-040353-01). Dcp1a&Dcp2 double knockdown were achieved by injecting a mixture of the above siRNAs.
| | Sample_growth_protocol_ch1 | Microinjected oocytes were cultured in CZB with milrinone for 20 h and then transferred to milrinone-free medium and allowed to mature to MII for 18 h. The MII eggs were then used for RNA extraction and microarray analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from pools of 30 MII eggs per samples using Pico-Pure RNA Isolation kit (Acturus).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA samples were amplified and labelled according to Affymetrix protocol GeneChip Eukaryotic Small Sample Target Labelling Assay Version II.
| | Sample_hyb_protocol | Standard Affymetrix procedures
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | The data were loaded into R software and normalized using AffyPLM package of the Bioconductor software. GCRMA algorithm was used for normalization. The values lower than 10.0 represent detection absent (with detection call P-value P>0.05).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM667nnn/GSM667582/suppl/GSM667582.CEL.gz
| | Sample_series_id | GSE27049
| | Sample_data_row_count | 45101
| | |
|
GSM667583 | GPL1261 |
|
Dcp1a siRNA_rep1
|
MII eggs injected with Dcp1a siRNA
|
strain: CF1
condition: Dcp1a siRNA
developmental stage: MII egg
|
Gene expression data from in vitro matured mouse MII eggs injected with Dcp1a siRNA
|
| Sample_geo_accession | GSM667583
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Feb 03 2011
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Full-grown GV oocytes were injected with Dcp1a siRNA (Dharmacon, On-TARGETplus SMARTpool, L-065144-01) or Dcp2 siRNA (Dharmacon, On-TARGETplus SMARTpool L-040353-01). Dcp1a&Dcp2 double knockdown were achieved by injecting a mixture of the above siRNAs.
| | Sample_growth_protocol_ch1 | Microinjected oocytes were cultured in CZB with milrinone for 20 h and then transferred to milrinone-free medium and allowed to mature to MII for 18 h. The MII eggs were then used for RNA extraction and microarray analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from pools of 30 MII eggs per samples using Pico-Pure RNA Isolation kit (Acturus).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA samples were amplified and labelled according to Affymetrix protocol GeneChip Eukaryotic Small Sample Target Labelling Assay Version II.
| | Sample_hyb_protocol | Standard Affymetrix procedures
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | The data were loaded into R software and normalized using AffyPLM package of the Bioconductor software. GCRMA algorithm was used for normalization. The values lower than 10.0 represent detection absent (with detection call P-value P>0.05).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM667nnn/GSM667583/suppl/GSM667583.CEL.gz
| | Sample_series_id | GSE27049
| | Sample_data_row_count | 45101
| | |
|
GSM667584 | GPL1261 |
|
Dcp1a siRNA_rep2
|
MII eggs injected with Dcp1a siRNA
|
strain: CF1
condition: Dcp1a siRNA
developmental stage: MII egg
|
Gene expression data from in vitro matured mouse MII eggs injected with Dcp1a siRNA
|
| Sample_geo_accession | GSM667584
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Feb 03 2011
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Full-grown GV oocytes were injected with Dcp1a siRNA (Dharmacon, On-TARGETplus SMARTpool, L-065144-01) or Dcp2 siRNA (Dharmacon, On-TARGETplus SMARTpool L-040353-01). Dcp1a&Dcp2 double knockdown were achieved by injecting a mixture of the above siRNAs.
| | Sample_growth_protocol_ch1 | Microinjected oocytes were cultured in CZB with milrinone for 20 h and then transferred to milrinone-free medium and allowed to mature to MII for 18 h. The MII eggs were then used for RNA extraction and microarray analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from pools of 30 MII eggs per samples using Pico-Pure RNA Isolation kit (Acturus).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA samples were amplified and labelled according to Affymetrix protocol GeneChip Eukaryotic Small Sample Target Labelling Assay Version II.
| | Sample_hyb_protocol | Standard Affymetrix procedures
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | The data were loaded into R software and normalized using AffyPLM package of the Bioconductor software. GCRMA algorithm was used for normalization. The values lower than 10.0 represent detection absent (with detection call P-value P>0.05).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM667nnn/GSM667584/suppl/GSM667584.CEL.gz
| | Sample_series_id | GSE27049
| | Sample_data_row_count | 45101
| | |
|
GSM667585 | GPL1261 |
|
Dcp1a siRNA_rep3
|
MII eggs injected with Dcp1a siRNA
|
strain: CF1
condition: Dcp1a siRNA
developmental stage: MII egg
|
Gene expression data from in vitro matured mouse MII eggs injected with Dcp1a siRNA
|
| Sample_geo_accession | GSM667585
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Feb 03 2011
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Full-grown GV oocytes were injected with Dcp1a siRNA (Dharmacon, On-TARGETplus SMARTpool, L-065144-01) or Dcp2 siRNA (Dharmacon, On-TARGETplus SMARTpool L-040353-01). Dcp1a&Dcp2 double knockdown were achieved by injecting a mixture of the above siRNAs.
| | Sample_growth_protocol_ch1 | Microinjected oocytes were cultured in CZB with milrinone for 20 h and then transferred to milrinone-free medium and allowed to mature to MII for 18 h. The MII eggs were then used for RNA extraction and microarray analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from pools of 30 MII eggs per samples using Pico-Pure RNA Isolation kit (Acturus).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA samples were amplified and labelled according to Affymetrix protocol GeneChip Eukaryotic Small Sample Target Labelling Assay Version II.
| | Sample_hyb_protocol | Standard Affymetrix procedures
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | The data were loaded into R software and normalized using AffyPLM package of the Bioconductor software. GCRMA algorithm was used for normalization. The values lower than 10.0 represent detection absent (with detection call P-value P>0.05).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM667nnn/GSM667585/suppl/GSM667585.CEL.gz
| | Sample_series_id | GSE27049
| | Sample_data_row_count | 45101
| | |
|
GSM667586 | GPL1261 |
|
Dcp2 siRNA_rep1
|
MII eggs injected with Dcp2 siRNA
|
strain: CF1
condition: Dcp2 siRNA
developmental stage: MII egg
|
Gene expression data from in vitro matured mouse MII eggs injected with Dcp2 siRNA
|
| Sample_geo_accession | GSM667586
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Feb 03 2011
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Full-grown GV oocytes were injected with Dcp1a siRNA (Dharmacon, On-TARGETplus SMARTpool, L-065144-01) or Dcp2 siRNA (Dharmacon, On-TARGETplus SMARTpool L-040353-01). Dcp1a&Dcp2 double knockdown were achieved by injecting a mixture of the above siRNAs.
| | Sample_growth_protocol_ch1 | Microinjected oocytes were cultured in CZB with milrinone for 20 h and then transferred to milrinone-free medium and allowed to mature to MII for 18 h. The MII eggs were then used for RNA extraction and microarray analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from pools of 30 MII eggs per samples using Pico-Pure RNA Isolation kit (Acturus).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA samples were amplified and labelled according to Affymetrix protocol GeneChip Eukaryotic Small Sample Target Labelling Assay Version II.
| | Sample_hyb_protocol | Standard Affymetrix procedures
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | The data were loaded into R software and normalized using AffyPLM package of the Bioconductor software. GCRMA algorithm was used for normalization. The values lower than 10.0 represent detection absent (with detection call P-value P>0.05).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM667nnn/GSM667586/suppl/GSM667586.CEL.gz
| | Sample_series_id | GSE27049
| | Sample_data_row_count | 45101
| | |
|
GSM667587 | GPL1261 |
|
Dcp2 siRNA_rep2
|
MII eggs injected with Dcp2 siRNA
|
strain: CF1
condition: Dcp2 siRNA
developmental stage: MII egg
|
Gene expression data from in vitro matured mouse MII eggs injected with Dcp2 siRNA
|
| Sample_geo_accession | GSM667587
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Feb 03 2011
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Full-grown GV oocytes were injected with Dcp1a siRNA (Dharmacon, On-TARGETplus SMARTpool, L-065144-01) or Dcp2 siRNA (Dharmacon, On-TARGETplus SMARTpool L-040353-01). Dcp1a&Dcp2 double knockdown were achieved by injecting a mixture of the above siRNAs.
| | Sample_growth_protocol_ch1 | Microinjected oocytes were cultured in CZB with milrinone for 20 h and then transferred to milrinone-free medium and allowed to mature to MII for 18 h. The MII eggs were then used for RNA extraction and microarray analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from pools of 30 MII eggs per samples using Pico-Pure RNA Isolation kit (Acturus).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA samples were amplified and labelled according to Affymetrix protocol GeneChip Eukaryotic Small Sample Target Labelling Assay Version II.
| | Sample_hyb_protocol | Standard Affymetrix procedures
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | The data were loaded into R software and normalized using AffyPLM package of the Bioconductor software. GCRMA algorithm was used for normalization. The values lower than 10.0 represent detection absent (with detection call P-value P>0.05).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM667nnn/GSM667587/suppl/GSM667587.CEL.gz
| | Sample_series_id | GSE27049
| | Sample_data_row_count | 45101
| | |
|
GSM667588 | GPL1261 |
|
Dcp2 siRNA_rep3
|
MII eggs injected with Dcp2 siRNA
|
strain: CF1
condition: Dcp2 siRNA
developmental stage: MII egg
|
Gene expression data from in vitro matured mouse MII eggs injected with Dcp2 siRNA
|
| Sample_geo_accession | GSM667588
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Feb 03 2011
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Full-grown GV oocytes were injected with Dcp1a siRNA (Dharmacon, On-TARGETplus SMARTpool, L-065144-01) or Dcp2 siRNA (Dharmacon, On-TARGETplus SMARTpool L-040353-01). Dcp1a&Dcp2 double knockdown were achieved by injecting a mixture of the above siRNAs.
| | Sample_growth_protocol_ch1 | Microinjected oocytes were cultured in CZB with milrinone for 20 h and then transferred to milrinone-free medium and allowed to mature to MII for 18 h. The MII eggs were then used for RNA extraction and microarray analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from pools of 30 MII eggs per samples using Pico-Pure RNA Isolation kit (Acturus).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA samples were amplified and labelled according to Affymetrix protocol GeneChip Eukaryotic Small Sample Target Labelling Assay Version II.
| | Sample_hyb_protocol | Standard Affymetrix procedures
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | The data were loaded into R software and normalized using AffyPLM package of the Bioconductor software. GCRMA algorithm was used for normalization. The values lower than 10.0 represent detection absent (with detection call P-value P>0.05).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM667nnn/GSM667588/suppl/GSM667588.CEL.gz
| | Sample_series_id | GSE27049
| | Sample_data_row_count | 45101
| | |
|
GSM667589 | GPL1261 |
|
Dcp1a+Dcp2 siRNA_rep1
|
MII eggs injected with Dcp1a+Dcp2 siRNAs
|
strain: CF1
condition: Dcp1a+Dcp2 siRNA
developmental stage: MII egg
|
Gene expression data from in vitro matured mouse MII eggs injected with Dcp1a+Dcp2 siRNAs
|
| Sample_geo_accession | GSM667589
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Feb 03 2011
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Full-grown GV oocytes were injected with Dcp1a siRNA (Dharmacon, On-TARGETplus SMARTpool, L-065144-01) or Dcp2 siRNA (Dharmacon, On-TARGETplus SMARTpool L-040353-01). Dcp1a&Dcp2 double knockdown were achieved by injecting a mixture of the above siRNAs.
| | Sample_growth_protocol_ch1 | Microinjected oocytes were cultured in CZB with milrinone for 20 h and then transferred to milrinone-free medium and allowed to mature to MII for 18 h. The MII eggs were then used for RNA extraction and microarray analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from pools of 30 MII eggs per samples using Pico-Pure RNA Isolation kit (Acturus).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA samples were amplified and labelled according to Affymetrix protocol GeneChip Eukaryotic Small Sample Target Labelling Assay Version II.
| | Sample_hyb_protocol | Standard Affymetrix procedures
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | The data were loaded into R software and normalized using AffyPLM package of the Bioconductor software. GCRMA algorithm was used for normalization. The values lower than 10.0 represent detection absent (with detection call P-value P>0.05).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM667nnn/GSM667589/suppl/GSM667589.CEL.gz
| | Sample_series_id | GSE27049
| | Sample_data_row_count | 45101
| | |
|
GSM667590 | GPL1261 |
|
Dcp1a+Dcp2 siRNA_rep2
|
MII eggs injected with Dcp1a+Dcp2 siRNAs
|
strain: CF1
condition: Dcp1a+Dcp2 siRNA
developmental stage: MII egg
|
Gene expression data from in vitro matured mouse MII eggs injected with Dcp1a+Dcp2 siRNAs
|
| Sample_geo_accession | GSM667590
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Feb 03 2011
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Full-grown GV oocytes were injected with Dcp1a siRNA (Dharmacon, On-TARGETplus SMARTpool, L-065144-01) or Dcp2 siRNA (Dharmacon, On-TARGETplus SMARTpool L-040353-01). Dcp1a&Dcp2 double knockdown were achieved by injecting a mixture of the above siRNAs.
| | Sample_growth_protocol_ch1 | Microinjected oocytes were cultured in CZB with milrinone for 20 h and then transferred to milrinone-free medium and allowed to mature to MII for 18 h. The MII eggs were then used for RNA extraction and microarray analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from pools of 30 MII eggs per samples using Pico-Pure RNA Isolation kit (Acturus).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA samples were amplified and labelled according to Affymetrix protocol GeneChip Eukaryotic Small Sample Target Labelling Assay Version II.
| | Sample_hyb_protocol | Standard Affymetrix procedures
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | The data were loaded into R software and normalized using AffyPLM package of the Bioconductor software. GCRMA algorithm was used for normalization. The values lower than 10.0 represent detection absent (with detection call P-value P>0.05).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM667nnn/GSM667590/suppl/GSM667590.CEL.gz
| | Sample_series_id | GSE27049
| | Sample_data_row_count | 45101
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GSM667591 | GPL1261 |
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Dcp1a+Dcp2 siRNA_rep3
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MII eggs injected with Dcp1a+Dcp2 siRNAs
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strain: CF1
condition: Dcp1a+Dcp2 siRNA
developmental stage: MII egg
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Gene expression data from in vitro matured mouse MII eggs injected with Dcp1a+Dcp2 siRNAs
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| Sample_geo_accession | GSM667591
| | Sample_status | Public on Feb 06 2013
| | Sample_submission_date | Feb 03 2011
| | Sample_last_update_date | Feb 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Full-grown GV oocytes were injected with Dcp1a siRNA (Dharmacon, On-TARGETplus SMARTpool, L-065144-01) or Dcp2 siRNA (Dharmacon, On-TARGETplus SMARTpool L-040353-01). Dcp1a&Dcp2 double knockdown were achieved by injecting a mixture of the above siRNAs.
| | Sample_growth_protocol_ch1 | Microinjected oocytes were cultured in CZB with milrinone for 20 h and then transferred to milrinone-free medium and allowed to mature to MII for 18 h. The MII eggs were then used for RNA extraction and microarray analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from pools of 30 MII eggs per samples using Pico-Pure RNA Isolation kit (Acturus).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA samples were amplified and labelled according to Affymetrix protocol GeneChip Eukaryotic Small Sample Target Labelling Assay Version II.
| | Sample_hyb_protocol | Standard Affymetrix procedures
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | The data were loaded into R software and normalized using AffyPLM package of the Bioconductor software. GCRMA algorithm was used for normalization. The values lower than 10.0 represent detection absent (with detection call P-value P>0.05).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM667nnn/GSM667591/suppl/GSM667591.CEL.gz
| | Sample_series_id | GSE27049
| | Sample_data_row_count | 45101
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