Search results for the GEO ID: GSE27071  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM668101 | GPL570 |
|
Monocyte cell line, control 1
|
Monocyte cell line (CRL-9853)
|
cell line: CRL-9853
cell type: monocyte
condition: serum starved
|
Gene expression data from serum stimulation of a human monocyte cell line
|
| Sample_geo_accession | GSM668101
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Feb 04 2011
| | Sample_last_update_date | May 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | After 96 hours the bottles were marked into 3 groups and annotated control, serum + inhibitor and serum only. 15 ul of DMSO was added to control and serum only bottles. 15 ul of 10mM U0196 (inhibitor) dissolved in DMSO were added to serum + inhibitor bottles. 2 hours later, after 98 hours, 1.6 ml of fetal calf serum were added to serum + inhibitor and serum only. After 120 hours each bottle were split equally into 2 centrifuge tubes and spun down.
| | Sample_growth_protocol_ch1 | The monocytes were started up growing in Optimen + 10% FCS + antibiotics. At time 0 hours the monocytes were divided into 13 bottles with 3 x 10E6 cells in each bottle. 15 ml of Optimen medium + 10% FCS + antibiotics was added to each bottle. After 80 hours the medium was changed to 15 ml of Optimen medium + antibiotics (no FSC).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted with the NucleoSpin® RNA/Protein mini kit (Macherey-Nagel, Düren, Germany) in accordance with the manufactures protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In accordance with the Affymetrix protocol and the one cycle eukaryotic target labelling assay, double-stranded cDNA was synthesized from total RNA and an IVT reaction was subsequently performed to produce biotin-labelled cRNA from the cDNA.
| | Sample_hyb_protocol | The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA) and herring sperm DNA. In this experiment, the Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied. The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythirn (SAPE).
| | Sample_scan_protocol | Scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
| | Sample_data_processing | Scaled and normalized using robust multi-array analysis (RMA) in the statistical software R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Morten,,Hansen
| | Sample_contact_department | Department of Cellular and Melocular Medicine
| | Sample_contact_institute | Faculty of Health Sciences
| | Sample_contact_address | Blegdamsvej 3B
| | Sample_contact_city | Copenhagen
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM668nnn/GSM668101/suppl/GSM668101.CEL.gz
| | Sample_series_id | GSE27071
| | Sample_data_row_count | 54613
| | |
|
GSM668102 | GPL570 |
|
Monocyte cell line, control 2
|
Monocyte cell line (CRL-9853)
|
cell line: CRL-9853
cell type: monocyte
condition: serum starved
|
Gene expression data from serum stimulation of a human monocyte cell line
|
| Sample_geo_accession | GSM668102
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Feb 04 2011
| | Sample_last_update_date | May 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | After 96 hours the bottles were marked into 3 groups and annotated control, serum + inhibitor and serum only. 15 ul of DMSO was added to control and serum only bottles. 15 ul of 10mM U0196 (inhibitor) dissolved in DMSO were added to serum + inhibitor bottles. 2 hours later, after 98 hours, 1.6 ml of fetal calf serum were added to serum + inhibitor and serum only. After 120 hours each bottle were split equally into 2 centrifuge tubes and spun down.
| | Sample_growth_protocol_ch1 | The monocytes were started up growing in Optimen + 10% FCS + antibiotics. At time 0 hours the monocytes were divided into 13 bottles with 3 x 10E6 cells in each bottle. 15 ml of Optimen medium + 10% FCS + antibiotics was added to each bottle. After 80 hours the medium was changed to 15 ml of Optimen medium + antibiotics (no FSC).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted with the NucleoSpin® RNA/Protein mini kit (Macherey-Nagel, Düren, Germany) in accordance with the manufactures protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In accordance with the Affymetrix protocol and the one cycle eukaryotic target labelling assay, double-stranded cDNA was synthesized from total RNA and an IVT reaction was subsequently performed to produce biotin-labelled cRNA from the cDNA.
| | Sample_hyb_protocol | The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA) and herring sperm DNA. In this experiment, the Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied. The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythirn (SAPE).
| | Sample_scan_protocol | Scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
| | Sample_data_processing | Scaled and normalized using robust multi-array analysis (RMA) in the statistical software R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Morten,,Hansen
| | Sample_contact_department | Department of Cellular and Melocular Medicine
| | Sample_contact_institute | Faculty of Health Sciences
| | Sample_contact_address | Blegdamsvej 3B
| | Sample_contact_city | Copenhagen
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM668nnn/GSM668102/suppl/GSM668102.CEL.gz
| | Sample_series_id | GSE27071
| | Sample_data_row_count | 54613
| | |
|
GSM668103 | GPL570 |
|
Monocyte cell line, control 3
|
Monocyte cell line (CRL-9853)
|
cell line: CRL-9853
cell type: monocyte
condition: serum starved
|
Gene expression data from serum stimulation of a human monocyte cell line
|
| Sample_geo_accession | GSM668103
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Feb 04 2011
| | Sample_last_update_date | May 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | After 96 hours the bottles were marked into 3 groups and annotated control, serum + inhibitor and serum only. 15 ul of DMSO was added to control and serum only bottles. 15 ul of 10mM U0196 (inhibitor) dissolved in DMSO were added to serum + inhibitor bottles. 2 hours later, after 98 hours, 1.6 ml of fetal calf serum were added to serum + inhibitor and serum only. After 120 hours each bottle were split equally into 2 centrifuge tubes and spun down.
| | Sample_growth_protocol_ch1 | The monocytes were started up growing in Optimen + 10% FCS + antibiotics. At time 0 hours the monocytes were divided into 13 bottles with 3 x 10E6 cells in each bottle. 15 ml of Optimen medium + 10% FCS + antibiotics was added to each bottle. After 80 hours the medium was changed to 15 ml of Optimen medium + antibiotics (no FSC).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted with the NucleoSpin® RNA/Protein mini kit (Macherey-Nagel, Düren, Germany) in accordance with the manufactures protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In accordance with the Affymetrix protocol and the one cycle eukaryotic target labelling assay, double-stranded cDNA was synthesized from total RNA and an IVT reaction was subsequently performed to produce biotin-labelled cRNA from the cDNA.
| | Sample_hyb_protocol | The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA) and herring sperm DNA. In this experiment, the Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied. The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythirn (SAPE).
| | Sample_scan_protocol | Scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
| | Sample_data_processing | Scaled and normalized using robust multi-array analysis (RMA) in the statistical software R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Morten,,Hansen
| | Sample_contact_department | Department of Cellular and Melocular Medicine
| | Sample_contact_institute | Faculty of Health Sciences
| | Sample_contact_address | Blegdamsvej 3B
| | Sample_contact_city | Copenhagen
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM668nnn/GSM668103/suppl/GSM668103.CEL.gz
| | Sample_series_id | GSE27071
| | Sample_data_row_count | 54613
| | |
|
GSM668104 | GPL570 |
|
Monocyte cell line, control 4
|
Monocyte cell line (CRL-9853)
|
cell line: CRL-9853
cell type: monocyte
condition: serum starved
|
Gene expression data from serum stimulation of a human monocyte cell line
|
| Sample_geo_accession | GSM668104
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Feb 04 2011
| | Sample_last_update_date | May 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | After 96 hours the bottles were marked into 3 groups and annotated control, serum + inhibitor and serum only. 15 ul of DMSO was added to control and serum only bottles. 15 ul of 10mM U0196 (inhibitor) dissolved in DMSO were added to serum + inhibitor bottles. 2 hours later, after 98 hours, 1.6 ml of fetal calf serum were added to serum + inhibitor and serum only. After 120 hours each bottle were split equally into 2 centrifuge tubes and spun down.
| | Sample_growth_protocol_ch1 | The monocytes were started up growing in Optimen + 10% FCS + antibiotics. At time 0 hours the monocytes were divided into 13 bottles with 3 x 10E6 cells in each bottle. 15 ml of Optimen medium + 10% FCS + antibiotics was added to each bottle. After 80 hours the medium was changed to 15 ml of Optimen medium + antibiotics (no FSC).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted with the NucleoSpin® RNA/Protein mini kit (Macherey-Nagel, Düren, Germany) in accordance with the manufactures protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In accordance with the Affymetrix protocol and the one cycle eukaryotic target labelling assay, double-stranded cDNA was synthesized from total RNA and an IVT reaction was subsequently performed to produce biotin-labelled cRNA from the cDNA.
| | Sample_hyb_protocol | The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA) and herring sperm DNA. In this experiment, the Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied. The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythirn (SAPE).
| | Sample_scan_protocol | Scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
| | Sample_data_processing | Scaled and normalized using robust multi-array analysis (RMA) in the statistical software R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Morten,,Hansen
| | Sample_contact_department | Department of Cellular and Melocular Medicine
| | Sample_contact_institute | Faculty of Health Sciences
| | Sample_contact_address | Blegdamsvej 3B
| | Sample_contact_city | Copenhagen
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM668nnn/GSM668104/suppl/GSM668104.CEL.gz
| | Sample_series_id | GSE27071
| | Sample_data_row_count | 54613
| | |
|
GSM668105 | GPL570 |
|
Monocyte cell line, control 5
|
Monocyte cell line (CRL-9853)
|
cell line: CRL-9853
cell type: monocyte
condition: serum starved
|
Gene expression data from serum stimulation of a human monocyte cell line
|
| Sample_geo_accession | GSM668105
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Feb 04 2011
| | Sample_last_update_date | May 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | After 96 hours the bottles were marked into 3 groups and annotated control, serum + inhibitor and serum only. 15 ul of DMSO was added to control and serum only bottles. 15 ul of 10mM U0196 (inhibitor) dissolved in DMSO were added to serum + inhibitor bottles. 2 hours later, after 98 hours, 1.6 ml of fetal calf serum were added to serum + inhibitor and serum only. After 120 hours each bottle were split equally into 2 centrifuge tubes and spun down.
| | Sample_growth_protocol_ch1 | The monocytes were started up growing in Optimen + 10% FCS + antibiotics. At time 0 hours the monocytes were divided into 13 bottles with 3 x 10E6 cells in each bottle. 15 ml of Optimen medium + 10% FCS + antibiotics was added to each bottle. After 80 hours the medium was changed to 15 ml of Optimen medium + antibiotics (no FSC).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted with the NucleoSpin® RNA/Protein mini kit (Macherey-Nagel, Düren, Germany) in accordance with the manufactures protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In accordance with the Affymetrix protocol and the one cycle eukaryotic target labelling assay, double-stranded cDNA was synthesized from total RNA and an IVT reaction was subsequently performed to produce biotin-labelled cRNA from the cDNA.
| | Sample_hyb_protocol | The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA) and herring sperm DNA. In this experiment, the Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied. The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythirn (SAPE).
| | Sample_scan_protocol | Scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
| | Sample_data_processing | Scaled and normalized using robust multi-array analysis (RMA) in the statistical software R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Morten,,Hansen
| | Sample_contact_department | Department of Cellular and Melocular Medicine
| | Sample_contact_institute | Faculty of Health Sciences
| | Sample_contact_address | Blegdamsvej 3B
| | Sample_contact_city | Copenhagen
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM668nnn/GSM668105/suppl/GSM668105.CEL.gz
| | Sample_series_id | GSE27071
| | Sample_data_row_count | 54613
| | |
|
GSM668106 | GPL570 |
|
Monocyte cell line, serum and inhibitor 1
|
Monocyte cell line (CRL-9853)
|
cell line: CRL-9853
cell type: monocyte
condition: Added serum and Erk-1/2 inhibitor U0126
|
Gene expression data from serum stimulation of a human monocyte cell line
|
| Sample_geo_accession | GSM668106
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Feb 04 2011
| | Sample_last_update_date | May 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | After 96 hours the bottles were marked into 3 groups and annotated control, serum + inhibitor and serum only. 15 ul of DMSO was added to control and serum only bottles. 15 ul of 10mM U0196 (inhibitor) dissolved in DMSO were added to serum + inhibitor bottles. 2 hours later, after 98 hours, 1.6 ml of fetal calf serum were added to serum + inhibitor and serum only. After 120 hours each bottle were split equally into 2 centrifuge tubes and spun down.
| | Sample_growth_protocol_ch1 | The monocytes were started up growing in Optimen + 10% FCS + antibiotics. At time 0 hours the monocytes were divided into 13 bottles with 3 x 10E6 cells in each bottle. 15 ml of Optimen medium + 10% FCS + antibiotics was added to each bottle. After 80 hours the medium was changed to 15 ml of Optimen medium + antibiotics (no FSC).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted with the NucleoSpin® RNA/Protein mini kit (Macherey-Nagel, Düren, Germany) in accordance with the manufactures protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In accordance with the Affymetrix protocol and the one cycle eukaryotic target labelling assay, double-stranded cDNA was synthesized from total RNA and an IVT reaction was subsequently performed to produce biotin-labelled cRNA from the cDNA.
| | Sample_hyb_protocol | The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA) and herring sperm DNA. In this experiment, the Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied. The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythirn (SAPE).
| | Sample_scan_protocol | Scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
| | Sample_data_processing | Scaled and normalized using robust multi-array analysis (RMA) in the statistical software R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Morten,,Hansen
| | Sample_contact_department | Department of Cellular and Melocular Medicine
| | Sample_contact_institute | Faculty of Health Sciences
| | Sample_contact_address | Blegdamsvej 3B
| | Sample_contact_city | Copenhagen
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM668nnn/GSM668106/suppl/GSM668106.CEL.gz
| | Sample_series_id | GSE27071
| | Sample_data_row_count | 54613
| | |
|
GSM668107 | GPL570 |
|
Monocyte cell line, serum and inhibitor 2
|
Monocyte cell line (CRL-9853)
|
cell line: CRL-9853
cell type: monocyte
condition: Added serum and Erk-1/2 inhibitor U0126
|
Gene expression data from serum stimulation of a human monocyte cell line
|
| Sample_geo_accession | GSM668107
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Feb 04 2011
| | Sample_last_update_date | May 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | After 96 hours the bottles were marked into 3 groups and annotated control, serum + inhibitor and serum only. 15 ul of DMSO was added to control and serum only bottles. 15 ul of 10mM U0196 (inhibitor) dissolved in DMSO were added to serum + inhibitor bottles. 2 hours later, after 98 hours, 1.6 ml of fetal calf serum were added to serum + inhibitor and serum only. After 120 hours each bottle were split equally into 2 centrifuge tubes and spun down.
| | Sample_growth_protocol_ch1 | The monocytes were started up growing in Optimen + 10% FCS + antibiotics. At time 0 hours the monocytes were divided into 13 bottles with 3 x 10E6 cells in each bottle. 15 ml of Optimen medium + 10% FCS + antibiotics was added to each bottle. After 80 hours the medium was changed to 15 ml of Optimen medium + antibiotics (no FSC).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted with the NucleoSpin® RNA/Protein mini kit (Macherey-Nagel, Düren, Germany) in accordance with the manufactures protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In accordance with the Affymetrix protocol and the one cycle eukaryotic target labelling assay, double-stranded cDNA was synthesized from total RNA and an IVT reaction was subsequently performed to produce biotin-labelled cRNA from the cDNA.
| | Sample_hyb_protocol | The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA) and herring sperm DNA. In this experiment, the Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied. The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythirn (SAPE).
| | Sample_scan_protocol | Scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
| | Sample_data_processing | Scaled and normalized using robust multi-array analysis (RMA) in the statistical software R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Morten,,Hansen
| | Sample_contact_department | Department of Cellular and Melocular Medicine
| | Sample_contact_institute | Faculty of Health Sciences
| | Sample_contact_address | Blegdamsvej 3B
| | Sample_contact_city | Copenhagen
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM668nnn/GSM668107/suppl/GSM668107.CEL.gz
| | Sample_series_id | GSE27071
| | Sample_data_row_count | 54613
| | |
|
GSM668108 | GPL570 |
|
Monocyte cell line, serum and inhibitor 3
|
Monocyte cell line (CRL-9853)
|
cell line: CRL-9853
cell type: monocyte
condition: Added serum and Erk-1/2 inhibitor U0126
|
Gene expression data from serum stimulation of a human monocyte cell line
|
| Sample_geo_accession | GSM668108
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Feb 04 2011
| | Sample_last_update_date | May 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | After 96 hours the bottles were marked into 3 groups and annotated control, serum + inhibitor and serum only. 15 ul of DMSO was added to control and serum only bottles. 15 ul of 10mM U0196 (inhibitor) dissolved in DMSO were added to serum + inhibitor bottles. 2 hours later, after 98 hours, 1.6 ml of fetal calf serum were added to serum + inhibitor and serum only. After 120 hours each bottle were split equally into 2 centrifuge tubes and spun down.
| | Sample_growth_protocol_ch1 | The monocytes were started up growing in Optimen + 10% FCS + antibiotics. At time 0 hours the monocytes were divided into 13 bottles with 3 x 10E6 cells in each bottle. 15 ml of Optimen medium + 10% FCS + antibiotics was added to each bottle. After 80 hours the medium was changed to 15 ml of Optimen medium + antibiotics (no FSC).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted with the NucleoSpin® RNA/Protein mini kit (Macherey-Nagel, Düren, Germany) in accordance with the manufactures protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In accordance with the Affymetrix protocol and the one cycle eukaryotic target labelling assay, double-stranded cDNA was synthesized from total RNA and an IVT reaction was subsequently performed to produce biotin-labelled cRNA from the cDNA.
| | Sample_hyb_protocol | The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA) and herring sperm DNA. In this experiment, the Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied. The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythirn (SAPE).
| | Sample_scan_protocol | Scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
| | Sample_data_processing | Scaled and normalized using robust multi-array analysis (RMA) in the statistical software R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Morten,,Hansen
| | Sample_contact_department | Department of Cellular and Melocular Medicine
| | Sample_contact_institute | Faculty of Health Sciences
| | Sample_contact_address | Blegdamsvej 3B
| | Sample_contact_city | Copenhagen
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM668nnn/GSM668108/suppl/GSM668108.CEL.gz
| | Sample_series_id | GSE27071
| | Sample_data_row_count | 54613
| | |
|
GSM668109 | GPL570 |
|
Monocyte cell line, serum and inhibitor 4
|
Monocyte cell line (CRL-9853)
|
cell line: CRL-9853
cell type: monocyte
condition: Added serum and Erk-1/2 inhibitor U0126
|
Gene expression data from serum stimulation of a human monocyte cell line
|
| Sample_geo_accession | GSM668109
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Feb 04 2011
| | Sample_last_update_date | May 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | After 96 hours the bottles were marked into 3 groups and annotated control, serum + inhibitor and serum only. 15 ul of DMSO was added to control and serum only bottles. 15 ul of 10mM U0196 (inhibitor) dissolved in DMSO were added to serum + inhibitor bottles. 2 hours later, after 98 hours, 1.6 ml of fetal calf serum were added to serum + inhibitor and serum only. After 120 hours each bottle were split equally into 2 centrifuge tubes and spun down.
| | Sample_growth_protocol_ch1 | The monocytes were started up growing in Optimen + 10% FCS + antibiotics. At time 0 hours the monocytes were divided into 13 bottles with 3 x 10E6 cells in each bottle. 15 ml of Optimen medium + 10% FCS + antibiotics was added to each bottle. After 80 hours the medium was changed to 15 ml of Optimen medium + antibiotics (no FSC).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted with the NucleoSpin® RNA/Protein mini kit (Macherey-Nagel, Düren, Germany) in accordance with the manufactures protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In accordance with the Affymetrix protocol and the one cycle eukaryotic target labelling assay, double-stranded cDNA was synthesized from total RNA and an IVT reaction was subsequently performed to produce biotin-labelled cRNA from the cDNA.
| | Sample_hyb_protocol | The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA) and herring sperm DNA. In this experiment, the Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied. The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythirn (SAPE).
| | Sample_scan_protocol | Scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
| | Sample_data_processing | Scaled and normalized using robust multi-array analysis (RMA) in the statistical software R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Morten,,Hansen
| | Sample_contact_department | Department of Cellular and Melocular Medicine
| | Sample_contact_institute | Faculty of Health Sciences
| | Sample_contact_address | Blegdamsvej 3B
| | Sample_contact_city | Copenhagen
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM668nnn/GSM668109/suppl/GSM668109.CEL.gz
| | Sample_series_id | GSE27071
| | Sample_data_row_count | 54613
| | |
|
GSM668110 | GPL570 |
|
Monocyte cell line, serum and inhibitor 5
|
Monocyte cell line (CRL-9853)
|
cell line: CRL-9853
cell type: monocyte
condition: Added serum and Erk-1/2 inhibitor U0126
|
Gene expression data from serum stimulation of a human monocyte cell line
|
| Sample_geo_accession | GSM668110
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Feb 04 2011
| | Sample_last_update_date | May 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | After 96 hours the bottles were marked into 3 groups and annotated control, serum + inhibitor and serum only. 15 ul of DMSO was added to control and serum only bottles. 15 ul of 10mM U0196 (inhibitor) dissolved in DMSO were added to serum + inhibitor bottles. 2 hours later, after 98 hours, 1.6 ml of fetal calf serum were added to serum + inhibitor and serum only. After 120 hours each bottle were split equally into 2 centrifuge tubes and spun down.
| | Sample_growth_protocol_ch1 | The monocytes were started up growing in Optimen + 10% FCS + antibiotics. At time 0 hours the monocytes were divided into 13 bottles with 3 x 10E6 cells in each bottle. 15 ml of Optimen medium + 10% FCS + antibiotics was added to each bottle. After 80 hours the medium was changed to 15 ml of Optimen medium + antibiotics (no FSC).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted with the NucleoSpin® RNA/Protein mini kit (Macherey-Nagel, Düren, Germany) in accordance with the manufactures protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In accordance with the Affymetrix protocol and the one cycle eukaryotic target labelling assay, double-stranded cDNA was synthesized from total RNA and an IVT reaction was subsequently performed to produce biotin-labelled cRNA from the cDNA.
| | Sample_hyb_protocol | The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA) and herring sperm DNA. In this experiment, the Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied. The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythirn (SAPE).
| | Sample_scan_protocol | Scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
| | Sample_data_processing | Scaled and normalized using robust multi-array analysis (RMA) in the statistical software R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Morten,,Hansen
| | Sample_contact_department | Department of Cellular and Melocular Medicine
| | Sample_contact_institute | Faculty of Health Sciences
| | Sample_contact_address | Blegdamsvej 3B
| | Sample_contact_city | Copenhagen
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM668nnn/GSM668110/suppl/GSM668110.CEL.gz
| | Sample_series_id | GSE27071
| | Sample_data_row_count | 54613
| | |
|
GSM668111 | GPL570 |
|
Monocyte cell line, serum only 1
|
Monocyte cell line (CRL-9853)
|
cell line: CRL-9853
cell type: monocyte
condition: Added serum only
|
Gene expression data from serum stimulation of a human monocyte cell line
|
| Sample_geo_accession | GSM668111
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Feb 04 2011
| | Sample_last_update_date | May 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | After 96 hours the bottles were marked into 3 groups and annotated control, serum + inhibitor and serum only. 15 ul of DMSO was added to control and serum only bottles. 15 ul of 10mM U0196 (inhibitor) dissolved in DMSO were added to serum + inhibitor bottles. 2 hours later, after 98 hours, 1.6 ml of fetal calf serum were added to serum + inhibitor and serum only. After 120 hours each bottle were split equally into 2 centrifuge tubes and spun down.
| | Sample_growth_protocol_ch1 | The monocytes were started up growing in Optimen + 10% FCS + antibiotics. At time 0 hours the monocytes were divided into 13 bottles with 3 x 10E6 cells in each bottle. 15 ml of Optimen medium + 10% FCS + antibiotics was added to each bottle. After 80 hours the medium was changed to 15 ml of Optimen medium + antibiotics (no FSC).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted with the NucleoSpin® RNA/Protein mini kit (Macherey-Nagel, Düren, Germany) in accordance with the manufactures protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In accordance with the Affymetrix protocol and the one cycle eukaryotic target labelling assay, double-stranded cDNA was synthesized from total RNA and an IVT reaction was subsequently performed to produce biotin-labelled cRNA from the cDNA.
| | Sample_hyb_protocol | The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA) and herring sperm DNA. In this experiment, the Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied. The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythirn (SAPE).
| | Sample_scan_protocol | Scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
| | Sample_data_processing | Scaled and normalized using robust multi-array analysis (RMA) in the statistical software R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Morten,,Hansen
| | Sample_contact_department | Department of Cellular and Melocular Medicine
| | Sample_contact_institute | Faculty of Health Sciences
| | Sample_contact_address | Blegdamsvej 3B
| | Sample_contact_city | Copenhagen
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM668nnn/GSM668111/suppl/GSM668111.CEL.gz
| | Sample_series_id | GSE27071
| | Sample_data_row_count | 54613
| | |
|
GSM668112 | GPL570 |
|
Monocyte cell line, serum only 2
|
Monocyte cell line (CRL-9853)
|
cell line: CRL-9853
cell type: monocyte
condition: Added serum only
|
Gene expression data from serum stimulation of a human monocyte cell line
|
| Sample_geo_accession | GSM668112
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Feb 04 2011
| | Sample_last_update_date | May 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | After 96 hours the bottles were marked into 3 groups and annotated control, serum + inhibitor and serum only. 15 ul of DMSO was added to control and serum only bottles. 15 ul of 10mM U0196 (inhibitor) dissolved in DMSO were added to serum + inhibitor bottles. 2 hours later, after 98 hours, 1.6 ml of fetal calf serum were added to serum + inhibitor and serum only. After 120 hours each bottle were split equally into 2 centrifuge tubes and spun down.
| | Sample_growth_protocol_ch1 | The monocytes were started up growing in Optimen + 10% FCS + antibiotics. At time 0 hours the monocytes were divided into 13 bottles with 3 x 10E6 cells in each bottle. 15 ml of Optimen medium + 10% FCS + antibiotics was added to each bottle. After 80 hours the medium was changed to 15 ml of Optimen medium + antibiotics (no FSC).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted with the NucleoSpin® RNA/Protein mini kit (Macherey-Nagel, Düren, Germany) in accordance with the manufactures protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In accordance with the Affymetrix protocol and the one cycle eukaryotic target labelling assay, double-stranded cDNA was synthesized from total RNA and an IVT reaction was subsequently performed to produce biotin-labelled cRNA from the cDNA.
| | Sample_hyb_protocol | The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA) and herring sperm DNA. In this experiment, the Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied. The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythirn (SAPE).
| | Sample_scan_protocol | Scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
| | Sample_data_processing | Scaled and normalized using robust multi-array analysis (RMA) in the statistical software R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Morten,,Hansen
| | Sample_contact_department | Department of Cellular and Melocular Medicine
| | Sample_contact_institute | Faculty of Health Sciences
| | Sample_contact_address | Blegdamsvej 3B
| | Sample_contact_city | Copenhagen
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM668nnn/GSM668112/suppl/GSM668112.CEL.gz
| | Sample_series_id | GSE27071
| | Sample_data_row_count | 54613
| | |
|
GSM668113 | GPL570 |
|
Monocyte cell line, serum only 3
|
Monocyte cell line (CRL-9853)
|
cell line: CRL-9853
cell type: monocyte
condition: Added serum only
|
Gene expression data from serum stimulation of a human monocyte cell line
|
| Sample_geo_accession | GSM668113
| | Sample_status | Public on May 01 2011
| | Sample_submission_date | Feb 04 2011
| | Sample_last_update_date | May 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | After 96 hours the bottles were marked into 3 groups and annotated control, serum + inhibitor and serum only. 15 ul of DMSO was added to control and serum only bottles. 15 ul of 10mM U0196 (inhibitor) dissolved in DMSO were added to serum + inhibitor bottles. 2 hours later, after 98 hours, 1.6 ml of fetal calf serum were added to serum + inhibitor and serum only. After 120 hours each bottle were split equally into 2 centrifuge tubes and spun down.
| | Sample_growth_protocol_ch1 | The monocytes were started up growing in Optimen + 10% FCS + antibiotics. At time 0 hours the monocytes were divided into 13 bottles with 3 x 10E6 cells in each bottle. 15 ml of Optimen medium + 10% FCS + antibiotics was added to each bottle. After 80 hours the medium was changed to 15 ml of Optimen medium + antibiotics (no FSC).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted with the NucleoSpin® RNA/Protein mini kit (Macherey-Nagel, Düren, Germany) in accordance with the manufactures protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | In accordance with the Affymetrix protocol and the one cycle eukaryotic target labelling assay, double-stranded cDNA was synthesized from total RNA and an IVT reaction was subsequently performed to produce biotin-labelled cRNA from the cDNA.
| | Sample_hyb_protocol | The cRNA was fragmented and a hybridization mix was prepared, which included the fragmented target, probe array controls, bovine serum albumin (BSA) and herring sperm DNA. In this experiment, the Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied. The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythirn (SAPE).
| | Sample_scan_protocol | Scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
| | Sample_data_processing | Scaled and normalized using robust multi-array analysis (RMA) in the statistical software R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Morten,,Hansen
| | Sample_contact_department | Department of Cellular and Melocular Medicine
| | Sample_contact_institute | Faculty of Health Sciences
| | Sample_contact_address | Blegdamsvej 3B
| | Sample_contact_city | Copenhagen
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM668nnn/GSM668113/suppl/GSM668113.CEL.gz
| | Sample_series_id | GSE27071
| | Sample_data_row_count | 54613
| | |
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