Search results for the GEO ID: GSE27291  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM674835 | GPL570 |
|
gd cell control time 0,donor 1
|
human blood sample, freshly isolated and purified
|
sample type: peripheral blood lymphocytes isolated prior to activation
cell type: TCRVgamma9+ gamma delta T cells
treatment: control
time: 0
individual: donor 1
|
Gene expression data from purified TCRVgamma9 positive cells
|
| Sample_geo_accession | GSM674835
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 14 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were activated by adding BrHPP (500 nM) ad day 0.
| | Sample_growth_protocol_ch1 | Cells were cultured in complete RPMI 1640 medium containing 2 mM L-glutamine, 100 µg/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate supplemented with 10% fetal calf serum and recombinant human IL-2 (100 IU/ml) added every three days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 µg total RNA from human cells, amplified and labeled following the one-Cycle Target Labeling protocol (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.
| | Sample_scan_protocol | The chips were then washed, scanned on the Affymetrix Fluidics Station 400 and analyzed with GeneChip Operating Software.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jean Jacques,,FOURNIE
| | Sample_contact_email | jean-jacques.fournie@inserm.fr
| | Sample_contact_phone | +33562748364
| | Sample_contact_fax | +3362744558
| | Sample_contact_laboratory | Cancer Research Center of Toulouse
| | Sample_contact_department | oncology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | CHU Purpan
| | Sample_contact_city | Toulouse
| | Sample_contact_zip/postal_code | 31024
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM674nnn/GSM674835/suppl/GSM674835_BC2902B_J0.CEL.gz
| | Sample_series_id | GSE27291
| | Sample_data_row_count | 54675
| | |
|
GSM674836 | GPL570 |
|
gd cell activation+6h,donor 1
|
human blood sample, freshly isolated and purified, 6 hours after in vitro activation
|
sample type: peripheral blood lymphocytes isolated and activated in vitro for 6 hrs
cell type: TCRVgamma9+ gamma delta T cells
treatment: BrHPP/IL2
time: 6 h
individual: donor 1
|
Gene expression data from purified TCRVgamma9 positive cells
|
| Sample_geo_accession | GSM674836
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 14 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were activated by adding BrHPP (500 nM) ad day 0.
| | Sample_growth_protocol_ch1 | Cells were cultured in complete RPMI 1640 medium containing 2 mM L-glutamine, 100 µg/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate supplemented with 10% fetal calf serum and recombinant human IL-2 (100 IU/ml) added every three days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 µg total RNA from human cells, amplified and labeled following the one-Cycle Target Labeling protocol (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.
| | Sample_scan_protocol | The chips were then washed, scanned on the Affymetrix Fluidics Station 400 and analyzed with GeneChip Operating Software.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jean Jacques,,FOURNIE
| | Sample_contact_email | jean-jacques.fournie@inserm.fr
| | Sample_contact_phone | +33562748364
| | Sample_contact_fax | +3362744558
| | Sample_contact_laboratory | Cancer Research Center of Toulouse
| | Sample_contact_department | oncology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | CHU Purpan
| | Sample_contact_city | Toulouse
| | Sample_contact_zip/postal_code | 31024
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM674nnn/GSM674836/suppl/GSM674836_BC2902B_6h.CEL.gz
| | Sample_series_id | GSE27291
| | Sample_data_row_count | 54675
| | |
|
GSM674837 | GPL570 |
|
gd cell activation+7day,donor 1
|
human blood sample, freshly isolated and purified, 7 days after in vitro activation
|
sample type: peripheral blood lymphocytes isolated and activated in vitro for 7 days
cell type: TCRVgamma9+ gamma delta T cells
treatment: BrHPP/IL2
time: 7 d
individual: donor 1
|
Gene expression data from purified TCRVgamma9 positive cells
|
| Sample_geo_accession | GSM674837
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 14 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were activated by adding BrHPP (500 nM) ad day 0.
| | Sample_growth_protocol_ch1 | Cells were cultured in complete RPMI 1640 medium containing 2 mM L-glutamine, 100 µg/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate supplemented with 10% fetal calf serum and recombinant human IL-2 (100 IU/ml) added every three days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 µg total RNA from human cells, amplified and labeled following the one-Cycle Target Labeling protocol (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.
| | Sample_scan_protocol | The chips were then washed, scanned on the Affymetrix Fluidics Station 400 and analyzed with GeneChip Operating Software.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jean Jacques,,FOURNIE
| | Sample_contact_email | jean-jacques.fournie@inserm.fr
| | Sample_contact_phone | +33562748364
| | Sample_contact_fax | +3362744558
| | Sample_contact_laboratory | Cancer Research Center of Toulouse
| | Sample_contact_department | oncology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | CHU Purpan
| | Sample_contact_city | Toulouse
| | Sample_contact_zip/postal_code | 31024
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM674nnn/GSM674837/suppl/GSM674837_BC2902B_J7.CEL.gz
| | Sample_series_id | GSE27291
| | Sample_data_row_count | 54675
| | |
|
GSM674838 | GPL570 |
|
gd cell control time 0,donor 2
|
human blood sample, freshly isolated and purified
|
sample type: peripheral blood lymphocytes isolated prior to activation
cell type: TCRVgamma9+ gamma delta T cells
treatment: control
time: 0
individual: donor 2
|
Gene expression data from purified TCRVgamma9 positive cells
|
| Sample_geo_accession | GSM674838
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 14 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were activated by adding BrHPP (500 nM) ad day 0.
| | Sample_growth_protocol_ch1 | Cells were cultured in complete RPMI 1640 medium containing 2 mM L-glutamine, 100 µg/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate supplemented with 10% fetal calf serum and recombinant human IL-2 (100 IU/ml) added every three days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 µg total RNA from human cells, amplified and labeled following the one-Cycle Target Labeling protocol (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.
| | Sample_scan_protocol | The chips were then washed, scanned on the Affymetrix Fluidics Station 400 and analyzed with GeneChip Operating Software.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jean Jacques,,FOURNIE
| | Sample_contact_email | jean-jacques.fournie@inserm.fr
| | Sample_contact_phone | +33562748364
| | Sample_contact_fax | +3362744558
| | Sample_contact_laboratory | Cancer Research Center of Toulouse
| | Sample_contact_department | oncology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | CHU Purpan
| | Sample_contact_city | Toulouse
| | Sample_contact_zip/postal_code | 31024
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM674nnn/GSM674838/suppl/GSM674838_BC2901A_J0.CEL.gz
| | Sample_series_id | GSE27291
| | Sample_data_row_count | 54675
| | |
|
GSM674839 | GPL570 |
|
gd cell activation+6h,donor 2
|
human blood sample, freshly isolated and purified, 6 hours after in vitro activation
|
sample type: peripheral blood lymphocytes isolated and activated in vitro for 6 hrs
cell type: TCRVgamma9+ gamma delta T cells
treatment: BrHPP/IL2
time: 6 h
individual: donor 2
|
Gene expression data from purified TCRVgamma9 positive cells
|
| Sample_geo_accession | GSM674839
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 14 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were activated by adding BrHPP (500 nM) ad day 0.
| | Sample_growth_protocol_ch1 | Cells were cultured in complete RPMI 1640 medium containing 2 mM L-glutamine, 100 µg/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate supplemented with 10% fetal calf serum and recombinant human IL-2 (100 IU/ml) added every three days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 µg total RNA from human cells, amplified and labeled following the one-Cycle Target Labeling protocol (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.
| | Sample_scan_protocol | The chips were then washed, scanned on the Affymetrix Fluidics Station 400 and analyzed with GeneChip Operating Software.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jean Jacques,,FOURNIE
| | Sample_contact_email | jean-jacques.fournie@inserm.fr
| | Sample_contact_phone | +33562748364
| | Sample_contact_fax | +3362744558
| | Sample_contact_laboratory | Cancer Research Center of Toulouse
| | Sample_contact_department | oncology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | CHU Purpan
| | Sample_contact_city | Toulouse
| | Sample_contact_zip/postal_code | 31024
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM674nnn/GSM674839/suppl/GSM674839_BC2901A_6h.CEL.gz
| | Sample_series_id | GSE27291
| | Sample_data_row_count | 54675
| | |
|
GSM674840 | GPL570 |
|
gd cell activation+7day,donor 2
|
human blood sample, freshly isolated and purified, 7 days after in vitro activation
|
sample type: peripheral blood lymphocytes isolated and activated in vitro for 7 days
cell type: TCRVgamma9+ gamma delta T cells
treatment: BrHPP/IL2
time: 7 d
individual: donor 2
|
Gene expression data from purified TCRVgamma9 positive cells
|
| Sample_geo_accession | GSM674840
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 14 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were activated by adding BrHPP (500 nM) ad day 0.
| | Sample_growth_protocol_ch1 | Cells were cultured in complete RPMI 1640 medium containing 2 mM L-glutamine, 100 µg/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate supplemented with 10% fetal calf serum and recombinant human IL-2 (100 IU/ml) added every three days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 µg total RNA from human cells, amplified and labeled following the one-Cycle Target Labeling protocol (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.
| | Sample_scan_protocol | The chips were then washed, scanned on the Affymetrix Fluidics Station 400 and analyzed with GeneChip Operating Software.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jean Jacques,,FOURNIE
| | Sample_contact_email | jean-jacques.fournie@inserm.fr
| | Sample_contact_phone | +33562748364
| | Sample_contact_fax | +3362744558
| | Sample_contact_laboratory | Cancer Research Center of Toulouse
| | Sample_contact_department | oncology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | CHU Purpan
| | Sample_contact_city | Toulouse
| | Sample_contact_zip/postal_code | 31024
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM674nnn/GSM674840/suppl/GSM674840_BC2901A_J7.CEL.gz
| | Sample_series_id | GSE27291
| | Sample_data_row_count | 54675
| | |
|
GSM674841 | GPL570 |
|
gd cell control time 0,donor 3
|
human blood sample, freshly isolated and purified
|
sample type: peripheral blood lymphocytes isolated prior to activation
cell type: TCRVgamma9+ gamma delta T cells
treatment: control
time: 0
individual: donor 3
|
Gene expression data from purified TCRVgamma9 positive cells
|
| Sample_geo_accession | GSM674841
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 14 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were activated by adding BrHPP (500 nM) ad day 0.
| | Sample_growth_protocol_ch1 | Cells were cultured in complete RPMI 1640 medium containing 2 mM L-glutamine, 100 µg/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate supplemented with 10% fetal calf serum and recombinant human IL-2 (100 IU/ml) added every three days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 µg total RNA from human cells, amplified and labeled following the one-Cycle Target Labeling protocol (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.
| | Sample_scan_protocol | The chips were then washed, scanned on the Affymetrix Fluidics Station 400 and analyzed with GeneChip Operating Software.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jean Jacques,,FOURNIE
| | Sample_contact_email | jean-jacques.fournie@inserm.fr
| | Sample_contact_phone | +33562748364
| | Sample_contact_fax | +3362744558
| | Sample_contact_laboratory | Cancer Research Center of Toulouse
| | Sample_contact_department | oncology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | CHU Purpan
| | Sample_contact_city | Toulouse
| | Sample_contact_zip/postal_code | 31024
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM674nnn/GSM674841/suppl/GSM674841_BC2802B_J0.CEL.gz
| | Sample_series_id | GSE27291
| | Sample_data_row_count | 54675
| | |
|
GSM674842 | GPL570 |
|
gd cell activation+6h,donor 3
|
human blood sample, freshly isolated and purified, 6 hours after in vitro activation
|
sample type: peripheral blood lymphocytes isolated and activated in vitro for 6 hrs
cell type: TCRVgamma9+ gamma delta T cells
treatment: BrHPP/IL2
time: 6 h
individual: donor 3
|
Gene expression data from purified TCRVgamma9 positive cells
|
| Sample_geo_accession | GSM674842
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 14 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were activated by adding BrHPP (500 nM) ad day 0.
| | Sample_growth_protocol_ch1 | Cells were cultured in complete RPMI 1640 medium containing 2 mM L-glutamine, 100 µg/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate supplemented with 10% fetal calf serum and recombinant human IL-2 (100 IU/ml) added every three days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 µg total RNA from human cells, amplified and labeled following the one-Cycle Target Labeling protocol (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.
| | Sample_scan_protocol | The chips were then washed, scanned on the Affymetrix Fluidics Station 400 and analyzed with GeneChip Operating Software.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jean Jacques,,FOURNIE
| | Sample_contact_email | jean-jacques.fournie@inserm.fr
| | Sample_contact_phone | +33562748364
| | Sample_contact_fax | +3362744558
| | Sample_contact_laboratory | Cancer Research Center of Toulouse
| | Sample_contact_department | oncology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | CHU Purpan
| | Sample_contact_city | Toulouse
| | Sample_contact_zip/postal_code | 31024
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM674nnn/GSM674842/suppl/GSM674842_BC2802B_6h.CEL.gz
| | Sample_series_id | GSE27291
| | Sample_data_row_count | 54675
| | |
|
GSM674843 | GPL570 |
|
gd cell activation+7day,donor 3
|
human blood sample, freshly isolated and purified, 7 days after in vitro activation
|
sample type: peripheral blood lymphocytes isolated and activated in vitro for 7 days
cell type: TCRVgamma9+ gamma delta T cells
treatment: BrHPP/IL2
time: 7 d
individual: donor 3
|
Gene expression data from purified TCRVgamma9 positive cells
|
| Sample_geo_accession | GSM674843
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 14 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were activated by adding BrHPP (500 nM) ad day 0.
| | Sample_growth_protocol_ch1 | Cells were cultured in complete RPMI 1640 medium containing 2 mM L-glutamine, 100 µg/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate supplemented with 10% fetal calf serum and recombinant human IL-2 (100 IU/ml) added every three days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 µg total RNA from human cells, amplified and labeled following the one-Cycle Target Labeling protocol (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.
| | Sample_scan_protocol | The chips were then washed, scanned on the Affymetrix Fluidics Station 400 and analyzed with GeneChip Operating Software.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jean Jacques,,FOURNIE
| | Sample_contact_email | jean-jacques.fournie@inserm.fr
| | Sample_contact_phone | +33562748364
| | Sample_contact_fax | +3362744558
| | Sample_contact_laboratory | Cancer Research Center of Toulouse
| | Sample_contact_department | oncology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | CHU Purpan
| | Sample_contact_city | Toulouse
| | Sample_contact_zip/postal_code | 31024
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM674nnn/GSM674843/suppl/GSM674843_BC2802B_J7.CEL.gz
| | Sample_series_id | GSE27291
| | Sample_data_row_count | 54675
| | |
|
GSM674844 | GPL570 |
|
gd cell control time 0,donor 4
|
human blood sample, freshly isolated and purified
|
sample type: peripheral blood lymphocytes isolated prior to activation
cell type: TCRVgamma9+ gamma delta T cells
treatment: control
time: 0
individual: donor 4
|
Gene expression data from purified TCRVgamma9 positive cells
|
| Sample_geo_accession | GSM674844
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 14 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were activated by adding BrHPP (500 nM) ad day 0.
| | Sample_growth_protocol_ch1 | Cells were cultured in complete RPMI 1640 medium containing 2 mM L-glutamine, 100 µg/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate supplemented with 10% fetal calf serum and recombinant human IL-2 (100 IU/ml) added every three days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 µg total RNA from human cells, amplified and labeled following the one-Cycle Target Labeling protocol (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.
| | Sample_scan_protocol | The chips were then washed, scanned on the Affymetrix Fluidics Station 400 and analyzed with GeneChip Operating Software.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jean Jacques,,FOURNIE
| | Sample_contact_email | jean-jacques.fournie@inserm.fr
| | Sample_contact_phone | +33562748364
| | Sample_contact_fax | +3362744558
| | Sample_contact_laboratory | Cancer Research Center of Toulouse
| | Sample_contact_department | oncology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | CHU Purpan
| | Sample_contact_city | Toulouse
| | Sample_contact_zip/postal_code | 31024
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM674nnn/GSM674844/suppl/GSM674844_BC2401B_J0.CEL.gz
| | Sample_series_id | GSE27291
| | Sample_data_row_count | 54675
| | |
|
GSM674845 | GPL570 |
|
gd cell activation+6h,donor 4
|
human blood sample, freshly isolated and purified, 6 hours after in vitro activation
|
sample type: peripheral blood lymphocytes isolated and activated in vitro for 6 hrs
cell type: TCRVgamma9+ gamma delta T cells
treatment: BrHPP/IL2
time: 6 h
individual: donor 4
|
Gene expression data from purified TCRVgamma9 positive cells
|
| Sample_geo_accession | GSM674845
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 14 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were activated by adding BrHPP (500 nM) ad day 0.
| | Sample_growth_protocol_ch1 | Cells were cultured in complete RPMI 1640 medium containing 2 mM L-glutamine, 100 µg/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate supplemented with 10% fetal calf serum and recombinant human IL-2 (100 IU/ml) added every three days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 µg total RNA from human cells, amplified and labeled following the one-Cycle Target Labeling protocol (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.
| | Sample_scan_protocol | The chips were then washed, scanned on the Affymetrix Fluidics Station 400 and analyzed with GeneChip Operating Software.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jean Jacques,,FOURNIE
| | Sample_contact_email | jean-jacques.fournie@inserm.fr
| | Sample_contact_phone | +33562748364
| | Sample_contact_fax | +3362744558
| | Sample_contact_laboratory | Cancer Research Center of Toulouse
| | Sample_contact_department | oncology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | CHU Purpan
| | Sample_contact_city | Toulouse
| | Sample_contact_zip/postal_code | 31024
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM674nnn/GSM674845/suppl/GSM674845_BC2401B_6h.CEL.gz
| | Sample_series_id | GSE27291
| | Sample_data_row_count | 54675
| | |
|
GSM674846 | GPL570 |
|
gd cell activation+7day,donor 4
|
human blood sample, freshly isolated and purified, 7 days after in vitro activation
|
sample type: peripheral blood lymphocytes isolated and activated in vitro for 7 days
cell type: TCRVgamma9+ gamma delta T cells
treatment: BrHPP/IL2
time: 7 d
individual: donor 4
|
Gene expression data from purified TCRVgamma9 positive cells
|
| Sample_geo_accession | GSM674846
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 14 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were activated by adding BrHPP (500 nM) ad day 0.
| | Sample_growth_protocol_ch1 | Cells were cultured in complete RPMI 1640 medium containing 2 mM L-glutamine, 100 µg/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate supplemented with 10% fetal calf serum and recombinant human IL-2 (100 IU/ml) added every three days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 µg total RNA from human cells, amplified and labeled following the one-Cycle Target Labeling protocol (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.
| | Sample_scan_protocol | The chips were then washed, scanned on the Affymetrix Fluidics Station 400 and analyzed with GeneChip Operating Software.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jean Jacques,,FOURNIE
| | Sample_contact_email | jean-jacques.fournie@inserm.fr
| | Sample_contact_phone | +33562748364
| | Sample_contact_fax | +3362744558
| | Sample_contact_laboratory | Cancer Research Center of Toulouse
| | Sample_contact_department | oncology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | CHU Purpan
| | Sample_contact_city | Toulouse
| | Sample_contact_zip/postal_code | 31024
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM674nnn/GSM674846/suppl/GSM674846_BC2401B_J7.CEL.gz
| | Sample_series_id | GSE27291
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
