Search results for the GEO ID: GSE27316  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM675223 | GPL570 |
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HEK293_control plasmid_rep1
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HEK293 cells transfected with control plasmid
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cell type: HEK293 cells
transfection: control plasmid
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01-Hek-0ng-1
Gene expression data from cultured HEK293 cells 24 hours after transfection with control plasmid
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| Sample_geo_accession | GSM675223
| | Sample_status | Public on Oct 11 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Oct 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293 and HeLa cells grown in a 6-well plate were transfected with 500ng control parental MosIR plasmid expressing EGFP or with 500ng MosIR plasmid expressing EGFP with 3'UTR containing long inverted repeat derived from mouse c-Mos coding sequence.
| | Sample_growth_protocol_ch1 | HEK293 and HeLa cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen) supplemented with 10 % FCS (Invitrogen), penicillin (100 U/mL, Invitrogen), and streptomycin (100 µg/mL, Invitrogen) at 37 °C and 5 % CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 or HeLa cells in a confluent 6-well dish 24 hours post-transfection using Absolutely RNA Miniprep Kit (Stratagene).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 µg) from each replicate was reverse transcribed with the Affymetrix cDNA synthesis kit and cRNA was produced by in vitro transcription (IVT) by T7 RNA polymerase using the Affymetrix IVT kit as per manufacturer's instructions.
| | Sample_hyb_protocol | Biotinylated cRNA (20 µg) was fragmented by heating with magnesium (as per Affymetrix's instructions) and 15 µg of fragmented cRNA was hybridized to Human U133 plus 2.0 GeneChips™.
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | All arrays were normalized using the GCRMA algorithm with quantile normalization composed of 100 bins using Genedata's Refiner application. Per chip normalization was performed by scaling the median of the genes called present (detection P-value < 0.04) to a value of 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675223/suppl/GSM675223.CEL.gz
| | Sample_series_id | GSE27316
| | Sample_data_row_count | 54675
| | |
|
GSM675224 | GPL570 |
|
HEK293_control plasmid_rep2
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HEK293 cells transfected with control plasmid
|
cell type: HEK293 cells
transfection: control plasmid
|
02-Hek-0ng-2
Gene expression data from cultured HEK293 cells 24 hours after transfection with control plasmid
|
| Sample_geo_accession | GSM675224
| | Sample_status | Public on Oct 11 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Oct 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293 and HeLa cells grown in a 6-well plate were transfected with 500ng control parental MosIR plasmid expressing EGFP or with 500ng MosIR plasmid expressing EGFP with 3'UTR containing long inverted repeat derived from mouse c-Mos coding sequence.
| | Sample_growth_protocol_ch1 | HEK293 and HeLa cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen) supplemented with 10 % FCS (Invitrogen), penicillin (100 U/mL, Invitrogen), and streptomycin (100 µg/mL, Invitrogen) at 37 °C and 5 % CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 or HeLa cells in a confluent 6-well dish 24 hours post-transfection using Absolutely RNA Miniprep Kit (Stratagene).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 µg) from each replicate was reverse transcribed with the Affymetrix cDNA synthesis kit and cRNA was produced by in vitro transcription (IVT) by T7 RNA polymerase using the Affymetrix IVT kit as per manufacturer's instructions.
| | Sample_hyb_protocol | Biotinylated cRNA (20 µg) was fragmented by heating with magnesium (as per Affymetrix's instructions) and 15 µg of fragmented cRNA was hybridized to Human U133 plus 2.0 GeneChips™.
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | All arrays were normalized using the GCRMA algorithm with quantile normalization composed of 100 bins using Genedata's Refiner application. Per chip normalization was performed by scaling the median of the genes called present (detection P-value < 0.04) to a value of 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675224/suppl/GSM675224.CEL.gz
| | Sample_series_id | GSE27316
| | Sample_data_row_count | 54675
| | |
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GSM675225 | GPL570 |
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HEK293_MosIR plasmid_rep1
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HEK293 cells transfected with MosIR plasmid
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cell type: HEK293 cells
transfection: MosIR plasmid
|
03-Hek-500ng-1
Gene expression data from cultured HEK293 cells 24 hours after transfection with MosIR plasmid
|
| Sample_geo_accession | GSM675225
| | Sample_status | Public on Oct 11 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Oct 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293 and HeLa cells grown in a 6-well plate were transfected with 500ng control parental MosIR plasmid expressing EGFP or with 500ng MosIR plasmid expressing EGFP with 3'UTR containing long inverted repeat derived from mouse c-Mos coding sequence.
| | Sample_growth_protocol_ch1 | HEK293 and HeLa cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen) supplemented with 10 % FCS (Invitrogen), penicillin (100 U/mL, Invitrogen), and streptomycin (100 µg/mL, Invitrogen) at 37 °C and 5 % CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 or HeLa cells in a confluent 6-well dish 24 hours post-transfection using Absolutely RNA Miniprep Kit (Stratagene).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 µg) from each replicate was reverse transcribed with the Affymetrix cDNA synthesis kit and cRNA was produced by in vitro transcription (IVT) by T7 RNA polymerase using the Affymetrix IVT kit as per manufacturer's instructions.
| | Sample_hyb_protocol | Biotinylated cRNA (20 µg) was fragmented by heating with magnesium (as per Affymetrix's instructions) and 15 µg of fragmented cRNA was hybridized to Human U133 plus 2.0 GeneChips™.
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | All arrays were normalized using the GCRMA algorithm with quantile normalization composed of 100 bins using Genedata's Refiner application. Per chip normalization was performed by scaling the median of the genes called present (detection P-value < 0.04) to a value of 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675225/suppl/GSM675225.CEL.gz
| | Sample_series_id | GSE27316
| | Sample_data_row_count | 54675
| | |
|
GSM675226 | GPL570 |
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HEK293_MosIR plasmid_rep2
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HEK293 cells transfected with MosIR plasmid
|
cell type: HEK293 cells
transfection: MosIR plasmid
|
04-Hek-500ng-2
Gene expression data from cultured HEK293 cells 24 hours after transfection with MosIR plasmid
|
| Sample_geo_accession | GSM675226
| | Sample_status | Public on Oct 11 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Oct 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293 and HeLa cells grown in a 6-well plate were transfected with 500ng control parental MosIR plasmid expressing EGFP or with 500ng MosIR plasmid expressing EGFP with 3'UTR containing long inverted repeat derived from mouse c-Mos coding sequence.
| | Sample_growth_protocol_ch1 | HEK293 and HeLa cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen) supplemented with 10 % FCS (Invitrogen), penicillin (100 U/mL, Invitrogen), and streptomycin (100 µg/mL, Invitrogen) at 37 °C and 5 % CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 or HeLa cells in a confluent 6-well dish 24 hours post-transfection using Absolutely RNA Miniprep Kit (Stratagene).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 µg) from each replicate was reverse transcribed with the Affymetrix cDNA synthesis kit and cRNA was produced by in vitro transcription (IVT) by T7 RNA polymerase using the Affymetrix IVT kit as per manufacturer's instructions.
| | Sample_hyb_protocol | Biotinylated cRNA (20 µg) was fragmented by heating with magnesium (as per Affymetrix's instructions) and 15 µg of fragmented cRNA was hybridized to Human U133 plus 2.0 GeneChips™.
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | All arrays were normalized using the GCRMA algorithm with quantile normalization composed of 100 bins using Genedata's Refiner application. Per chip normalization was performed by scaling the median of the genes called present (detection P-value < 0.04) to a value of 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675226/suppl/GSM675226.CEL.gz
| | Sample_series_id | GSE27316
| | Sample_data_row_count | 54675
| | |
|
GSM675227 | GPL570 |
|
HeLa_control plasmid_rep1
|
HeLa cells transfected with control plasmid
|
cell type: HeLa cells
transfection: control plasmid
|
05-HeLa-0ng-1
Gene expression data from cultured HeLa cells 24 hours after transfection with control plasmid
|
| Sample_geo_accession | GSM675227
| | Sample_status | Public on Oct 11 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Oct 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293 and HeLa cells grown in a 6-well plate were transfected with 500ng control parental MosIR plasmid expressing EGFP or with 500ng MosIR plasmid expressing EGFP with 3'UTR containing long inverted repeat derived from mouse c-Mos coding sequence.
| | Sample_growth_protocol_ch1 | HEK293 and HeLa cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen) supplemented with 10 % FCS (Invitrogen), penicillin (100 U/mL, Invitrogen), and streptomycin (100 µg/mL, Invitrogen) at 37 °C and 5 % CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 or HeLa cells in a confluent 6-well dish 24 hours post-transfection using Absolutely RNA Miniprep Kit (Stratagene).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 µg) from each replicate was reverse transcribed with the Affymetrix cDNA synthesis kit and cRNA was produced by in vitro transcription (IVT) by T7 RNA polymerase using the Affymetrix IVT kit as per manufacturer's instructions.
| | Sample_hyb_protocol | Biotinylated cRNA (20 µg) was fragmented by heating with magnesium (as per Affymetrix's instructions) and 15 µg of fragmented cRNA was hybridized to Human U133 plus 2.0 GeneChips™.
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | All arrays were normalized using the GCRMA algorithm with quantile normalization composed of 100 bins using Genedata's Refiner application. Per chip normalization was performed by scaling the median of the genes called present (detection P-value < 0.04) to a value of 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675227/suppl/GSM675227.CEL.gz
| | Sample_series_id | GSE27316
| | Sample_data_row_count | 54675
| | |
|
GSM675228 | GPL570 |
|
HeLa_control plasmid_rep2
|
HeLa cells transfected with control plasmid
|
cell type: HeLa cells
transfection: control plasmid
|
06-HeLa-0ng-2
Gene expression data from cultured HeLa cells 24 hours after transfection with control plasmid
|
| Sample_geo_accession | GSM675228
| | Sample_status | Public on Oct 11 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Oct 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293 and HeLa cells grown in a 6-well plate were transfected with 500ng control parental MosIR plasmid expressing EGFP or with 500ng MosIR plasmid expressing EGFP with 3'UTR containing long inverted repeat derived from mouse c-Mos coding sequence.
| | Sample_growth_protocol_ch1 | HEK293 and HeLa cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen) supplemented with 10 % FCS (Invitrogen), penicillin (100 U/mL, Invitrogen), and streptomycin (100 µg/mL, Invitrogen) at 37 °C and 5 % CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 or HeLa cells in a confluent 6-well dish 24 hours post-transfection using Absolutely RNA Miniprep Kit (Stratagene).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 µg) from each replicate was reverse transcribed with the Affymetrix cDNA synthesis kit and cRNA was produced by in vitro transcription (IVT) by T7 RNA polymerase using the Affymetrix IVT kit as per manufacturer's instructions.
| | Sample_hyb_protocol | Biotinylated cRNA (20 µg) was fragmented by heating with magnesium (as per Affymetrix's instructions) and 15 µg of fragmented cRNA was hybridized to Human U133 plus 2.0 GeneChips™.
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | All arrays were normalized using the GCRMA algorithm with quantile normalization composed of 100 bins using Genedata's Refiner application. Per chip normalization was performed by scaling the median of the genes called present (detection P-value < 0.04) to a value of 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675228/suppl/GSM675228.CEL.gz
| | Sample_series_id | GSE27316
| | Sample_data_row_count | 54675
| | |
|
GSM675229 | GPL570 |
|
HeLa_MosIR plasmid_rep1
|
HeLa cells transfected with MosIR plasmid
|
cell type: HeLa cells
transfection: MosIR plasmid
|
07-HeLa-500ng-1
Gene expression data from cultured HeLa cells 24 hours after transfection with control plasmid
|
| Sample_geo_accession | GSM675229
| | Sample_status | Public on Oct 11 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Oct 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293 and HeLa cells grown in a 6-well plate were transfected with 500ng control parental MosIR plasmid expressing EGFP or with 500ng MosIR plasmid expressing EGFP with 3'UTR containing long inverted repeat derived from mouse c-Mos coding sequence.
| | Sample_growth_protocol_ch1 | HEK293 and HeLa cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen) supplemented with 10 % FCS (Invitrogen), penicillin (100 U/mL, Invitrogen), and streptomycin (100 µg/mL, Invitrogen) at 37 °C and 5 % CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 or HeLa cells in a confluent 6-well dish 24 hours post-transfection using Absolutely RNA Miniprep Kit (Stratagene).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 µg) from each replicate was reverse transcribed with the Affymetrix cDNA synthesis kit and cRNA was produced by in vitro transcription (IVT) by T7 RNA polymerase using the Affymetrix IVT kit as per manufacturer's instructions.
| | Sample_hyb_protocol | Biotinylated cRNA (20 µg) was fragmented by heating with magnesium (as per Affymetrix's instructions) and 15 µg of fragmented cRNA was hybridized to Human U133 plus 2.0 GeneChips™.
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | All arrays were normalized using the GCRMA algorithm with quantile normalization composed of 100 bins using Genedata's Refiner application. Per chip normalization was performed by scaling the median of the genes called present (detection P-value < 0.04) to a value of 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675229/suppl/GSM675229.CEL.gz
| | Sample_series_id | GSE27316
| | Sample_data_row_count | 54675
| | |
|
GSM675230 | GPL570 |
|
HeLa_MosIR plasmid_rep2
|
HeLa cells transfected with MosIR plasmid
|
cell type: HeLa cells
transfection: MosIR plasmid
|
08-HeLa-500ng-2
Gene expression data from cultured HeLa cells 24 hours after transfection with control plasmid
|
| Sample_geo_accession | GSM675230
| | Sample_status | Public on Oct 11 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Oct 11 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HEK293 and HeLa cells grown in a 6-well plate were transfected with 500ng control parental MosIR plasmid expressing EGFP or with 500ng MosIR plasmid expressing EGFP with 3'UTR containing long inverted repeat derived from mouse c-Mos coding sequence.
| | Sample_growth_protocol_ch1 | HEK293 and HeLa cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen) supplemented with 10 % FCS (Invitrogen), penicillin (100 U/mL, Invitrogen), and streptomycin (100 µg/mL, Invitrogen) at 37 °C and 5 % CO2 atmosphere.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 or HeLa cells in a confluent 6-well dish 24 hours post-transfection using Absolutely RNA Miniprep Kit (Stratagene).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (5 µg) from each replicate was reverse transcribed with the Affymetrix cDNA synthesis kit and cRNA was produced by in vitro transcription (IVT) by T7 RNA polymerase using the Affymetrix IVT kit as per manufacturer's instructions.
| | Sample_hyb_protocol | Biotinylated cRNA (20 µg) was fragmented by heating with magnesium (as per Affymetrix's instructions) and 15 µg of fragmented cRNA was hybridized to Human U133 plus 2.0 GeneChips™.
| | Sample_scan_protocol | Standard Affymetrix procedures
| | Sample_data_processing | All arrays were normalized using the GCRMA algorithm with quantile normalization composed of 100 bins using Genedata's Refiner application. Per chip normalization was performed by scaling the median of the genes called present (detection P-value < 0.04) to a value of 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Matyas,,Flemr
| | Sample_contact_institute | Institute of Molecular Genetics
| | Sample_contact_address | Videnska 1083
| | Sample_contact_city | Prague 4
| | Sample_contact_zip/postal_code | 14220
| | Sample_contact_country | Czech Republic
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675230/suppl/GSM675230.CEL.gz
| | Sample_series_id | GSE27316
| | Sample_data_row_count | 54675
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