Search results for the GEO ID: GSE27318  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM675399 | GPL570 |
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NESG1-overexpressed 2F4-5-8F NPC cells rep1
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NPC 5-8F cells with NESG1 overexpression
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cell type: NPC 5-8F cells
genotype/variation: NESG1 overexpression
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2F4-1
Gene expression data from NESG1-overexpressed NPC 5-8F cells
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| Sample_geo_accession | GSM675399
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4 NPC cells were harvested by trizol and preserved at -80℃
| | Sample_growth_protocol_ch1 | 4 NPC cells, including two from NESG1-negative C6-Ctr cells and two from NESG1-expressing 2F4 cells were cultured by 10% newborn calf serum (NBCS)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at -80℃ on GeneChip U133_Plus_2 (GPL570 platform)Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | weiyi,,fang
| | Sample_contact_email | fangweiyi1975@yahoo.com.cn
| | Sample_contact_institute | cancer institute
| | Sample_contact_address | tonghe road
| | Sample_contact_city | guangzhou
| | Sample_contact_zip/postal_code | 510515
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675399/suppl/GSM675399.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675399/suppl/GSM675399.CHP.gz
| | Sample_series_id | GSE27318
| | Sample_data_row_count | 54675
| | |
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GSM675400 | GPL570 |
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NESG1-overexpressed 2F4-5-8F NPC cells rep2
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NPC 5-8F cells with NESG1 overexpression
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cell type: NPC 5-8F cells
genotype/variation: NESG1 overexpression
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2F4-2
Gene expression data from NESG1-overexpressed NPC 5-8F cells
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| Sample_geo_accession | GSM675400
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4 NPC cells were harvested by trizol and preserved at -80℃
| | Sample_growth_protocol_ch1 | 4 NPC cells, including two from NESG1-negative C6-Ctr cells and two from NESG1-expressing 2F4 cells were cultured by 10% newborn calf serum (NBCS)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at -80℃ on GeneChip U133_Plus_2 (GPL570 platform)Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | weiyi,,fang
| | Sample_contact_email | fangweiyi1975@yahoo.com.cn
| | Sample_contact_institute | cancer institute
| | Sample_contact_address | tonghe road
| | Sample_contact_city | guangzhou
| | Sample_contact_zip/postal_code | 510515
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675400/suppl/GSM675400.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675400/suppl/GSM675400.CHP.gz
| | Sample_series_id | GSE27318
| | Sample_data_row_count | 54675
| | |
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GSM675401 | GPL570 |
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NESG1-negative C6-5-8F NPC cells rep1
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NPC 5-8F cells with NESG1-negative expression
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cell type: NPC 5-8F cells
genotype/variation: control
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C6-1
Gene expression data from NESG1-negative expression NPC 5-8F cells
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| Sample_geo_accession | GSM675401
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4 NPC cells were harvested by trizol and preserved at -80℃
| | Sample_growth_protocol_ch1 | 4 NPC cells, including two from NESG1-negative C6-Ctr cells and two from NESG1-expressing 2F4 cells were cultured by 10% newborn calf serum (NBCS)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at -80℃ on GeneChip U133_Plus_2 (GPL570 platform)Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | weiyi,,fang
| | Sample_contact_email | fangweiyi1975@yahoo.com.cn
| | Sample_contact_institute | cancer institute
| | Sample_contact_address | tonghe road
| | Sample_contact_city | guangzhou
| | Sample_contact_zip/postal_code | 510515
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675401/suppl/GSM675401.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675401/suppl/GSM675401.CHP.gz
| | Sample_series_id | GSE27318
| | Sample_data_row_count | 54675
| | |
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GSM675402 | GPL570 |
|
NESG1-negative C6-5-8F NPC cells rep2
|
NPC 5-8F cells with NESG1-negative expression
|
cell type: NPC 5-8F cells
genotype/variation: control
|
C6-2
Gene expression data from NESG1-negative expression NPC 5-8F cells
|
| Sample_geo_accession | GSM675402
| | Sample_status | Public on Feb 15 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Feb 15 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4 NPC cells were harvested by trizol and preserved at -80℃
| | Sample_growth_protocol_ch1 | 4 NPC cells, including two from NESG1-negative C6-Ctr cells and two from NESG1-expressing 2F4 cells were cultured by 10% newborn calf serum (NBCS)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at -80℃ on GeneChip U133_Plus_2 (GPL570 platform)Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | weiyi,,fang
| | Sample_contact_email | fangweiyi1975@yahoo.com.cn
| | Sample_contact_institute | cancer institute
| | Sample_contact_address | tonghe road
| | Sample_contact_city | guangzhou
| | Sample_contact_zip/postal_code | 510515
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675402/suppl/GSM675402.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675402/suppl/GSM675402.CHP.gz
| | Sample_series_id | GSE27318
| | Sample_data_row_count | 54675
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