Search results for the GEO ID: GSE27328  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM675631 | GPL570 |
|
SKOV3 cells without expressing LHR, replicate 1
|
control human ovarian cancer SKOV3 cell
|
cell line: SKOV-3
|
|
| Sample_geo_accession | GSM675631
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675631/suppl/GSM675631_S0252F005.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675632 | GPL570 |
|
SKOV3 cells without expressing LHR, replicate 2
|
control human ovarian cancer SKOV3 cell
|
cell line: SKOV-3
|
|
| Sample_geo_accession | GSM675632
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675632/suppl/GSM675632_S0252F011.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675633 | GPL570 |
|
SKOV3 cells without expressing LHR, replicate 3
|
control human ovarian cancer SKOV3 cell
|
cell line: SKOV-3
|
|
| Sample_geo_accession | GSM675633
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675633/suppl/GSM675633_S0252F006.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675634 | GPL570 |
|
SKOV3 cells tranfected to express LHR, without LH trearment, replicate 1
|
transfected SKOV3 cell with LHR expression
|
cell line: SKOV-3
transfection: LHR
|
|
| Sample_geo_accession | GSM675634
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675634/suppl/GSM675634_S0252F010.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675635 | GPL570 |
|
SKOV3 cells tranfected to express LHR, without LH trearment , replicate 2
|
transfected SKOV3 cell with LHR expression
|
cell line: SKOV-3
transfection: LHR
|
|
| Sample_geo_accession | GSM675635
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675635/suppl/GSM675635_S0252F013.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675636 | GPL570 |
|
SKOV3 cells tranfected to express LHR, without LH trearment, replicate 3
|
transfected SKOV3 cell with LHR expression
|
cell line: SKOV-3
transfection: LHR
|
|
| Sample_geo_accession | GSM675636
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675636/suppl/GSM675636_S0252F008.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675637 | GPL570 |
|
SKOV3 cells tranfected to express LHR, with LH treatment (50ng/ml, 1h), replicate 1
|
LH treated (1h) LHR+ SKOV3 cell
|
cell line: SKOV-3
transfection: LHR
treatment: LH treatment (50ng/ml)
time: 1h
|
|
| Sample_geo_accession | GSM675637
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675637/suppl/GSM675637_S0252F002.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675638 | GPL570 |
|
SKOV3 cells tranfected to express LHR, with LH treatment (50ng/ml, 1h) , replicate 2
|
LH treated (1h) LHR+ SKOV3 cell
|
cell line: SKOV-3
transfection: LHR
treatment: LH treatment (50ng/ml)
time: 1h
|
|
| Sample_geo_accession | GSM675638
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675638/suppl/GSM675638_S0252F001.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675639 | GPL570 |
|
SKOV3 cells tranfected to express LHR, with LH treatment (50ng/ml, 1h) , replicate 3
|
LH treated (1h) LHR+ SKOV3 cell
|
cell line: SKOV-3
transfection: LHR
treatment: LH treatment (50ng/ml)
time: 1h
|
|
| Sample_geo_accession | GSM675639
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675639/suppl/GSM675639_S0252F003.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675640 | GPL570 |
|
SKOV3 cells tranfected to express LHR, with LH treatment (50ng/ml, 4h) , replicate 1
|
LH treated (4h) LHR+ SKOV3 cell
|
cell line: SKOV-3
transfection: LHR
treatment: LH treatment (50ng/ml)
time: 4h
|
|
| Sample_geo_accession | GSM675640
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675640/suppl/GSM675640_S0252F018.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675641 | GPL570 |
|
SKOV3 cells tranfected to express LHR, with LH treatment (50ng/ml, 4h) , replicate 2
|
LH treated (4h) LHR+ SKOV3 cell
|
cell line: SKOV-3
transfection: LHR
treatment: LH treatment (50ng/ml)
time: 4h
|
|
| Sample_geo_accession | GSM675641
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675641/suppl/GSM675641_S0252F009.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675642 | GPL570 |
|
SKOV3 cells tranfected to express LHR, with LH treatment (50ng/ml, 4h) , replicate 3
|
LH treated (4h) LHR+ SKOV3 cell
|
cell line: SKOV-3
transfection: LHR
treatment: LH treatment (50ng/ml)
time: 4h
|
|
| Sample_geo_accession | GSM675642
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675642/suppl/GSM675642_S0252F004.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675643 | GPL570 |
|
SKOV3 cells tranfected to express LHR, with LH treatment (50ng/ml, 8h), replicate 1
|
LH treated (8h) LHR+ SKOV3 cell
|
cell line: SKOV-3
transfection: LHR
treatment: LH treatment (50ng/ml)
time: 8h
|
|
| Sample_geo_accession | GSM675643
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675643/suppl/GSM675643_S0252F015.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675644 | GPL570 |
|
SKOV3 cells tranfected to express LHR, with LH treatment (50ng/ml, 8h) , replicate 2
|
LH treated (8h) LHR+ SKOV3 cell
|
cell line: SKOV-3
transfection: LHR
treatment: LH treatment (50ng/ml)
time: 8h
|
|
| Sample_geo_accession | GSM675644
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675644/suppl/GSM675644_S0252F017.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675645 | GPL570 |
|
SKOV3 cells tranfected to express LHR, with LH treatment (50ng/ml, 8h) , replicate 3
|
LH treated (8h) LHR+ SKOV3 cell
|
cell line: SKOV-3
transfection: LHR
treatment: LH treatment (50ng/ml)
time: 8h
|
|
| Sample_geo_accession | GSM675645
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675645/suppl/GSM675645_S0252F007.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675646 | GPL570 |
|
SKOV3 cells tranfected to express LHR, with LH treatment (50ng/ml, 20h) , replicate 1
|
LH treated (20h) LHR+ SKOV3 cell
|
cell line: SKOV-3
transfection: LHR
treatment: LH treatment (50ng/ml)
time: 20h
|
|
| Sample_geo_accession | GSM675646
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675646/suppl/GSM675646_S0252F012.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675647 | GPL570 |
|
SKOV3 cells tranfected to express LHR, with LH treatment (50ng/ml, 20h) , replicate 2
|
LH treated (20h) LHR+ SKOV3 cell
|
cell line: SKOV-3
transfection: LHR
treatment: LH treatment (50ng/ml)
time: 20h
|
|
| Sample_geo_accession | GSM675647
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675647/suppl/GSM675647_S0252F014.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
GSM675648 | GPL570 |
|
SKOV3 cells tranfected to express LHR, with LH treatment (50ng/ml, 20h) , replicate 3
|
LH treated (20h) LHR+ SKOV3 cell
|
cell line: SKOV-3
transfection: LHR
treatment: LH treatment (50ng/ml)
time: 20h
|
|
| Sample_geo_accession | GSM675648
| | Sample_status | Public on Mar 01 2011
| | Sample_submission_date | Feb 15 2011
| | Sample_last_update_date | Mar 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | transfected with LHR and then treated with LH
| | Sample_growth_protocol_ch1 | cultured human ovarian cancer cell SKOV3
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the 18 SKOV-3 samples [14] and was amplified using the NuGEN™ Ovation™ RNA Amplification System V2.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
| | Sample_hyb_protocol | The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN guidelines for hybridization onto Affymetrix human genome U133 Plus2 Arrays.Following hybridization for 18 h at 45°C, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script
| | Sample_scan_protocol | GeneChip® Scanner 3000
| | Sample_data_processing | The raw probe intensities was normalized using the quartile normalization approach, and the PLIER method. was utilized to summarize the probe signal to both the exon- and gene-level expressions
| | Sample_platform_id | GPL570
| | Sample_contact_name | Juan,,Cui
| | Sample_contact_email | juancui@csbl.bmb.uga.edu
| | Sample_contact_phone | 706-542-9779
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Georgia
| | Sample_contact_address | 120 Green Street
| | Sample_contact_city | Athens
| | Sample_contact_zip/postal_code | 30602
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM675nnn/GSM675648/suppl/GSM675648_S0252F016.CEL.gz
| | Sample_series_id | GSE27328
| | Sample_data_row_count | 54675
| | |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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