Result

Search results for the GEO ID: GSE27429

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Source name

Description

Characteristics

GSM678120

GPL1261

tooth germs treated by 5E1, biological rep1

tooth germs affected by 5E1, biological rep1

strain: ICR tissue: mandibular molar tooth germs, embryos antibody transfer to embryo: intraperitoneal 5E1 injection to pregnant mice injection time: embryonic day 14 sampling time: at 24 hours after injection

Gene expression data from tooth germs at 24hours after injection time.

Sample_geo_accession	GSM678120
Sample_status	Public on Feb 22 2011
Sample_submission_date	Feb 21 2011
Sample_last_update_date	Feb 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	A single injection of 5E1 (10 mg/kg body weight), 40-1a (10 mg/kg body weight), cyclopamine (10 mg/kg body weight) or PBS (1 ml) was administered intraperitoneally to pregnant ICR mice at the stage of embryonic day 14. After one day tooth germs were dissected from mandibles in embryos.
Sample_growth_protocol_ch1	Embryos were collected from pregnant mice at 24 hours after injection.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. And then, extracted total RNA was purified using RNeasy min elute kit manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Sung-Won,,Cho
Sample_contact_email	chosome1@yuhs.ac
Sample_contact_laboratory	Anatomy and Developmental Biology
Sample_contact_department	Oral Biology
Sample_contact_institute	Yonsei University College of Dentistry
Sample_contact_address	Seongsanno 250, Seodaemun-gu
Sample_contact_city	Seoul
Sample_contact_zip/postal_code	120-752
Sample_contact_country	South Korea
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678120/suppl/GSM678120.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678120/suppl/GSM678120.CHP.gz
Sample_series_id	GSE27429
Sample_data_row_count	45101

GSM678121

GPL1261

tooth germs treated by 5E1, biological rep2

tooth germs affected by 5E1, biological rep2

Gene expression data from tooth germs at 24hours after injection time.

Sample_geo_accession	GSM678121
Sample_status	Public on Feb 22 2011
Sample_submission_date	Feb 21 2011
Sample_last_update_date	Feb 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	A single injection of 5E1 (10 mg/kg body weight), 40-1a (10 mg/kg body weight), cyclopamine (10 mg/kg body weight) or PBS (1 ml) was administered intraperitoneally to pregnant ICR mice at the stage of embryonic day 14. After one day tooth germs were dissected from mandibles in embryos.
Sample_growth_protocol_ch1	Embryos were collected from pregnant mice at 24 hours after injection.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. And then, extracted total RNA was purified using RNeasy min elute kit manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Sung-Won,,Cho
Sample_contact_email	chosome1@yuhs.ac
Sample_contact_laboratory	Anatomy and Developmental Biology
Sample_contact_department	Oral Biology
Sample_contact_institute	Yonsei University College of Dentistry
Sample_contact_address	Seongsanno 250, Seodaemun-gu
Sample_contact_city	Seoul
Sample_contact_zip/postal_code	120-752
Sample_contact_country	South Korea
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678121/suppl/GSM678121.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678121/suppl/GSM678121.CHP.gz
Sample_series_id	GSE27429
Sample_data_row_count	45101

GSM678122

GPL1261

tooth germs treated by 40-1a, biological rep1

tooth germs affected by 40-1a, biological rep1

strain: ICR tissue: mandibular molar tooth germs, embryos antibody transfer to embryo: intraperitoneal 40-1a injection to pregnant mice injection time: embryonic day 14 sampling time: at 24 hours after injection

Gene expression data from tooth germs at 24hours after injection time.

Sample_geo_accession	GSM678122
Sample_status	Public on Feb 22 2011
Sample_submission_date	Feb 21 2011
Sample_last_update_date	Feb 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	A single injection of 5E1 (10 mg/kg body weight), 40-1a (10 mg/kg body weight), cyclopamine (10 mg/kg body weight) or PBS (1 ml) was administered intraperitoneally to pregnant ICR mice at the stage of embryonic day 14. After one day tooth germs were dissected from mandibles in embryos.
Sample_growth_protocol_ch1	Embryos were collected from pregnant mice at 24 hours after injection.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. And then, extracted total RNA was purified using RNeasy min elute kit manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Sung-Won,,Cho
Sample_contact_email	chosome1@yuhs.ac
Sample_contact_laboratory	Anatomy and Developmental Biology
Sample_contact_department	Oral Biology
Sample_contact_institute	Yonsei University College of Dentistry
Sample_contact_address	Seongsanno 250, Seodaemun-gu
Sample_contact_city	Seoul
Sample_contact_zip/postal_code	120-752
Sample_contact_country	South Korea
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678122/suppl/GSM678122.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678122/suppl/GSM678122.CHP.gz
Sample_series_id	GSE27429
Sample_data_row_count	45101

GSM678123

GPL1261

tooth germs treated by 40-1a, biological rep2

tooth germs affected by 40-1a, biological rep2

Gene expression data from tooth germs at 24hours after injection time.

Sample_geo_accession	GSM678123
Sample_status	Public on Feb 22 2011
Sample_submission_date	Feb 21 2011
Sample_last_update_date	Feb 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	A single injection of 5E1 (10 mg/kg body weight), 40-1a (10 mg/kg body weight), cyclopamine (10 mg/kg body weight) or PBS (1 ml) was administered intraperitoneally to pregnant ICR mice at the stage of embryonic day 14. After one day tooth germs were dissected from mandibles in embryos.
Sample_growth_protocol_ch1	Embryos were collected from pregnant mice at 24 hours after injection.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. And then, extracted total RNA was purified using RNeasy min elute kit manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Sung-Won,,Cho
Sample_contact_email	chosome1@yuhs.ac
Sample_contact_laboratory	Anatomy and Developmental Biology
Sample_contact_department	Oral Biology
Sample_contact_institute	Yonsei University College of Dentistry
Sample_contact_address	Seongsanno 250, Seodaemun-gu
Sample_contact_city	Seoul
Sample_contact_zip/postal_code	120-752
Sample_contact_country	South Korea
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678123/suppl/GSM678123.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678123/suppl/GSM678123.CHP.gz
Sample_series_id	GSE27429
Sample_data_row_count	45101

GSM678124

GPL1261

tooth germs treated by cyclopamine, biological rep1

tooth germs affected by cyclopamine, biological rep1

strain: ICR tissue: mandibular molar tooth germs, embryos antibody transfer to embryo: intraperitoneal cyclopamine injection to pregnant mice injection time: embryonic day 14 sampling time: at 24 hours after injection

Gene expression data from tooth germs at 24hours after injection time.

Sample_geo_accession	GSM678124
Sample_status	Public on Feb 22 2011
Sample_submission_date	Feb 21 2011
Sample_last_update_date	Feb 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	A single injection of 5E1 (10 mg/kg body weight), 40-1a (10 mg/kg body weight), cyclopamine (10 mg/kg body weight) or PBS (1 ml) was administered intraperitoneally to pregnant ICR mice at the stage of embryonic day 14. After one day tooth germs were dissected from mandibles in embryos.
Sample_growth_protocol_ch1	Embryos were collected from pregnant mice at 24 hours after injection.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. And then, extracted total RNA was purified using RNeasy min elute kit manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Sung-Won,,Cho
Sample_contact_email	chosome1@yuhs.ac
Sample_contact_laboratory	Anatomy and Developmental Biology
Sample_contact_department	Oral Biology
Sample_contact_institute	Yonsei University College of Dentistry
Sample_contact_address	Seongsanno 250, Seodaemun-gu
Sample_contact_city	Seoul
Sample_contact_zip/postal_code	120-752
Sample_contact_country	South Korea
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678124/suppl/GSM678124.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678124/suppl/GSM678124.CHP.gz
Sample_series_id	GSE27429
Sample_data_row_count	45101

GSM678125

GPL1261

tooth germs treated by cyclopamine, biological rep2

tooth germs affected by cyclopamine, biological rep2

Gene expression data from tooth germs at 24hours after injection time.

Sample_geo_accession	GSM678125
Sample_status	Public on Feb 22 2011
Sample_submission_date	Feb 21 2011
Sample_last_update_date	Feb 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	A single injection of 5E1 (10 mg/kg body weight), 40-1a (10 mg/kg body weight), cyclopamine (10 mg/kg body weight) or PBS (1 ml) was administered intraperitoneally to pregnant ICR mice at the stage of embryonic day 14. After one day tooth germs were dissected from mandibles in embryos.
Sample_growth_protocol_ch1	Embryos were collected from pregnant mice at 24 hours after injection.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. And then, extracted total RNA was purified using RNeasy min elute kit manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Sung-Won,,Cho
Sample_contact_email	chosome1@yuhs.ac
Sample_contact_laboratory	Anatomy and Developmental Biology
Sample_contact_department	Oral Biology
Sample_contact_institute	Yonsei University College of Dentistry
Sample_contact_address	Seongsanno 250, Seodaemun-gu
Sample_contact_city	Seoul
Sample_contact_zip/postal_code	120-752
Sample_contact_country	South Korea
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678125/suppl/GSM678125.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678125/suppl/GSM678125.CHP.gz
Sample_series_id	GSE27429
Sample_data_row_count	45101

GSM678126

GPL1261

tooth germs treated by PBS, biological rep1

tooth germs affected by PBS, biological rep1

strain: ICR tissue: mandibular molar tooth germs, embryos antibody transfer to embryo: intraperitoneal PBS injection to pregnant mice injection time: embryonic day 14 sampling time: at 24 hours after injection

Gene expression data from tooth germs at 24hours after injection time.

Sample_geo_accession	GSM678126
Sample_status	Public on Feb 22 2011
Sample_submission_date	Feb 21 2011
Sample_last_update_date	Feb 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	A single injection of 5E1 (10 mg/kg body weight), 40-1a (10 mg/kg body weight), cyclopamine (10 mg/kg body weight) or PBS (1 ml) was administered intraperitoneally to pregnant ICR mice at the stage of embryonic day 14. After one day tooth germs were dissected from mandibles in embryos.
Sample_growth_protocol_ch1	Embryos were collected from pregnant mice at 24 hours after injection.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. And then, extracted total RNA was purified using RNeasy min elute kit manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Sung-Won,,Cho
Sample_contact_email	chosome1@yuhs.ac
Sample_contact_laboratory	Anatomy and Developmental Biology
Sample_contact_department	Oral Biology
Sample_contact_institute	Yonsei University College of Dentistry
Sample_contact_address	Seongsanno 250, Seodaemun-gu
Sample_contact_city	Seoul
Sample_contact_zip/postal_code	120-752
Sample_contact_country	South Korea
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678126/suppl/GSM678126.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678126/suppl/GSM678126.CHP.gz
Sample_series_id	GSE27429
Sample_data_row_count	45101

GSM678127

GPL1261

tooth germs treated by PBS, biological rep2

tooth germs affected by PBS, biological rep2

Gene expression data from tooth germs at 24hours after injection time.

Sample_geo_accession	GSM678127
Sample_status	Public on Feb 22 2011
Sample_submission_date	Feb 21 2011
Sample_last_update_date	Feb 25 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	A single injection of 5E1 (10 mg/kg body weight), 40-1a (10 mg/kg body weight), cyclopamine (10 mg/kg body weight) or PBS (1 ml) was administered intraperitoneally to pregnant ICR mice at the stage of embryonic day 14. After one day tooth germs were dissected from mandibles in embryos.
Sample_growth_protocol_ch1	Embryos were collected from pregnant mice at 24 hours after injection.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. And then, extracted total RNA was purified using RNeasy min elute kit manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, Affymetrix, P/N 702232 Rev. 3).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL1261
Sample_contact_name	Sung-Won,,Cho
Sample_contact_email	chosome1@yuhs.ac
Sample_contact_laboratory	Anatomy and Developmental Biology
Sample_contact_department	Oral Biology
Sample_contact_institute	Yonsei University College of Dentistry
Sample_contact_address	Seongsanno 250, Seodaemun-gu
Sample_contact_city	Seoul
Sample_contact_zip/postal_code	120-752
Sample_contact_country	South Korea
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678127/suppl/GSM678127.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678127/suppl/GSM678127.CHP.gz
Sample_series_id	GSE27429
Sample_data_row_count	45101

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