Result

Search results for the GEO ID: GSE27473

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM678802

GPL570

MCF7, biological rep1

MCF7 expressing Estrogen receptor alpha

knockdown: control cell line: MCF7

Gene expression data from MCF7 breast cancer cell line expressing Estrogen receptor

Sample_geo_accession	GSM678802
Sample_status	Public on May 01 2011
Sample_submission_date	Feb 23 2011
Sample_last_update_date	May 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Endocrine independent cell line pII was established by transfection of MCF7 with ER directed shRNA plasmid. Another transfected cell line designated E2 was established that had retained sensitivity to estrogen and tamoxifen (presumably as a result of failure to produce siRNA) was used as an additional control.
Sample_growth_protocol_ch1	MCF7 derived from the ATCC (American Type Culture Collection, (VA, USA) were maintained at 37˚C in a humidified atmosphere of 5% CO2 in DMEM supplemented with 10% foetal bovine serum (FBS), 600 μg/ml L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin and 6 ml/500 ml 100 x non-essential amino acids (Invitrogen, CA, USA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix Scanner 3000 7G
Sample_data_processing	The data were analyzed with Partek Genomics Suite V 6.5 for RMA background correction, quantile normalisation with GC content adjustment
Sample_platform_id	GPL570
Sample_contact_name	fahd,,Al-Mulla
Sample_contact_email	fahd@al-mulla.org
Sample_contact_phone	+96524986233
Sample_contact_fax	+96525338905
Sample_contact_laboratory	Molecular pathology
Sample_contact_department	Pathology
Sample_contact_institute	Kuwait University
Sample_contact_address	Faculty of medicine
Sample_contact_city	Kuwait
Sample_contact_zip/postal_code	13110
Sample_contact_country	Kuwait
Sample_contact_web_link	al-mulla.org
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678802/suppl/GSM678802_MCF1.CEL.gz
Sample_series_id	GSE27473
Sample_data_row_count	54675

GSM678803

GPL570

MCF7, biological rep2

MCF7 expressing Estrogen receptor alpha

knockdown: control cell line: MCF7

Gene expression data from MCF7 breast cancer cell line expressing Estrogen receptor

Sample_geo_accession	GSM678803
Sample_status	Public on May 01 2011
Sample_submission_date	Feb 23 2011
Sample_last_update_date	May 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Endocrine independent cell line pII was established by transfection of MCF7 with ER directed shRNA plasmid. Another transfected cell line designated E2 was established that had retained sensitivity to estrogen and tamoxifen (presumably as a result of failure to produce siRNA) was used as an additional control.
Sample_growth_protocol_ch1	MCF7 derived from the ATCC (American Type Culture Collection, (VA, USA) were maintained at 37˚C in a humidified atmosphere of 5% CO2 in DMEM supplemented with 10% foetal bovine serum (FBS), 600 μg/ml L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin and 6 ml/500 ml 100 x non-essential amino acids (Invitrogen, CA, USA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix Scanner 3000 7G
Sample_data_processing	The data were analyzed with Partek Genomics Suite V 6.5 for RMA background correction, quantile normalisation with GC content adjustment
Sample_platform_id	GPL570
Sample_contact_name	fahd,,Al-Mulla
Sample_contact_email	fahd@al-mulla.org
Sample_contact_phone	+96524986233
Sample_contact_fax	+96525338905
Sample_contact_laboratory	Molecular pathology
Sample_contact_department	Pathology
Sample_contact_institute	Kuwait University
Sample_contact_address	Faculty of medicine
Sample_contact_city	Kuwait
Sample_contact_zip/postal_code	13110
Sample_contact_country	Kuwait
Sample_contact_web_link	al-mulla.org
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678803/suppl/GSM678803_MCF2.CEL.gz
Sample_series_id	GSE27473
Sample_data_row_count	54675

GSM678804

GPL570

MCF7, biological rep3

MCF7 expressing Estrogen receptor alpha

knockdown: control cell line: MCF7

Gene expression data from MCF7 breast cancer cell line expressing Estrogen receptor

Sample_geo_accession	GSM678804
Sample_status	Public on May 01 2011
Sample_submission_date	Feb 23 2011
Sample_last_update_date	May 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Endocrine independent cell line pII was established by transfection of MCF7 with ER directed shRNA plasmid. Another transfected cell line designated E2 was established that had retained sensitivity to estrogen and tamoxifen (presumably as a result of failure to produce siRNA) was used as an additional control.
Sample_growth_protocol_ch1	MCF7 derived from the ATCC (American Type Culture Collection, (VA, USA) were maintained at 37˚C in a humidified atmosphere of 5% CO2 in DMEM supplemented with 10% foetal bovine serum (FBS), 600 μg/ml L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin and 6 ml/500 ml 100 x non-essential amino acids (Invitrogen, CA, USA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix Scanner 3000 7G
Sample_data_processing	The data were analyzed with Partek Genomics Suite V 6.5 for RMA background correction, quantile normalisation with GC content adjustment
Sample_platform_id	GPL570
Sample_contact_name	fahd,,Al-Mulla
Sample_contact_email	fahd@al-mulla.org
Sample_contact_phone	+96524986233
Sample_contact_fax	+96525338905
Sample_contact_laboratory	Molecular pathology
Sample_contact_department	Pathology
Sample_contact_institute	Kuwait University
Sample_contact_address	Faculty of medicine
Sample_contact_city	Kuwait
Sample_contact_zip/postal_code	13110
Sample_contact_country	Kuwait
Sample_contact_web_link	al-mulla.org
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678804/suppl/GSM678804_MCF3.CEL.gz
Sample_series_id	GSE27473
Sample_data_row_count	54675

GSM678805

GPL570

MCF7 silenced Estrogen receptor, biological rep1

MCF7 silenced Estrogen receptor alpha (PII)

knockdown: estrogen receptor alpha KD cell line: MCF7

Gene expression data from MCF7 breast cancer cell line with silenced Estrogen receptor

Sample_geo_accession	GSM678805
Sample_status	Public on May 01 2011
Sample_submission_date	Feb 23 2011
Sample_last_update_date	May 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Endocrine independent cell line pII was established by transfection of MCF7 with ER directed shRNA plasmid. Another transfected cell line designated E2 was established that had retained sensitivity to estrogen and tamoxifen (presumably as a result of failure to produce siRNA) was used as an additional control.
Sample_growth_protocol_ch1	MCF7 derived from the ATCC (American Type Culture Collection, (VA, USA) were maintained at 37˚C in a humidified atmosphere of 5% CO2 in DMEM supplemented with 10% foetal bovine serum (FBS), 600 μg/ml L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin and 6 ml/500 ml 100 x non-essential amino acids (Invitrogen, CA, USA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix Scanner 3000 7G
Sample_data_processing	The data were analyzed with Partek Genomics Suite V 6.5 for RMA background correction, quantile normalisation with GC content adjustment
Sample_platform_id	GPL570
Sample_contact_name	fahd,,Al-Mulla
Sample_contact_email	fahd@al-mulla.org
Sample_contact_phone	+96524986233
Sample_contact_fax	+96525338905
Sample_contact_laboratory	Molecular pathology
Sample_contact_department	Pathology
Sample_contact_institute	Kuwait University
Sample_contact_address	Faculty of medicine
Sample_contact_city	Kuwait
Sample_contact_zip/postal_code	13110
Sample_contact_country	Kuwait
Sample_contact_web_link	al-mulla.org
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678805/suppl/GSM678805_1P2.CEL.gz
Sample_series_id	GSE27473
Sample_data_row_count	54675

GSM678806

GPL570

MCF7 silenced Estrogen receptor, biological rep2

MCF7 silenced Estrogen receptor alpha (PII)

knockdown: estrogen receptor alpha KD cell line: MCF7

Gene expression data from MCF7 breast cancer cell line with silenced Estrogen receptor

Sample_geo_accession	GSM678806
Sample_status	Public on May 01 2011
Sample_submission_date	Feb 23 2011
Sample_last_update_date	May 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Endocrine independent cell line pII was established by transfection of MCF7 with ER directed shRNA plasmid. Another transfected cell line designated E2 was established that had retained sensitivity to estrogen and tamoxifen (presumably as a result of failure to produce siRNA) was used as an additional control.
Sample_growth_protocol_ch1	MCF7 derived from the ATCC (American Type Culture Collection, (VA, USA) were maintained at 37˚C in a humidified atmosphere of 5% CO2 in DMEM supplemented with 10% foetal bovine serum (FBS), 600 μg/ml L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin and 6 ml/500 ml 100 x non-essential amino acids (Invitrogen, CA, USA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix Scanner 3000 7G
Sample_data_processing	The data were analyzed with Partek Genomics Suite V 6.5 for RMA background correction, quantile normalisation with GC content adjustment
Sample_platform_id	GPL570
Sample_contact_name	fahd,,Al-Mulla
Sample_contact_email	fahd@al-mulla.org
Sample_contact_phone	+96524986233
Sample_contact_fax	+96525338905
Sample_contact_laboratory	Molecular pathology
Sample_contact_department	Pathology
Sample_contact_institute	Kuwait University
Sample_contact_address	Faculty of medicine
Sample_contact_city	Kuwait
Sample_contact_zip/postal_code	13110
Sample_contact_country	Kuwait
Sample_contact_web_link	al-mulla.org
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678806/suppl/GSM678806_2P2.CEL.gz
Sample_series_id	GSE27473
Sample_data_row_count	54675

GSM678807

GPL570

MCF7 silenced Estrogen receptor, biological rep3

MCF7 silenced Estrogen receptor alpha (PII)

knockdown: estrogen receptor alpha KD cell line: MCF7

Gene expression data from MCF7 breast cancer cell line with silenced Estrogen receptor

Sample_geo_accession	GSM678807
Sample_status	Public on May 01 2011
Sample_submission_date	Feb 23 2011
Sample_last_update_date	May 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Endocrine independent cell line pII was established by transfection of MCF7 with ER directed shRNA plasmid. Another transfected cell line designated E2 was established that had retained sensitivity to estrogen and tamoxifen (presumably as a result of failure to produce siRNA) was used as an additional control.
Sample_growth_protocol_ch1	MCF7 derived from the ATCC (American Type Culture Collection, (VA, USA) were maintained at 37˚C in a humidified atmosphere of 5% CO2 in DMEM supplemented with 10% foetal bovine serum (FBS), 600 μg/ml L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin and 6 ml/500 ml 100 x non-essential amino acids (Invitrogen, CA, USA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix Scanner 3000 7G
Sample_data_processing	The data were analyzed with Partek Genomics Suite V 6.5 for RMA background correction, quantile normalisation with GC content adjustment
Sample_platform_id	GPL570
Sample_contact_name	fahd,,Al-Mulla
Sample_contact_email	fahd@al-mulla.org
Sample_contact_phone	+96524986233
Sample_contact_fax	+96525338905
Sample_contact_laboratory	Molecular pathology
Sample_contact_department	Pathology
Sample_contact_institute	Kuwait University
Sample_contact_address	Faculty of medicine
Sample_contact_city	Kuwait
Sample_contact_zip/postal_code	13110
Sample_contact_country	Kuwait
Sample_contact_web_link	al-mulla.org
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM678nnn/GSM678807/suppl/GSM678807_3P2.CEL.gz
Sample_series_id	GSE27473
Sample_data_row_count	54675

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