Result

Search results for the GEO ID: GSE27494

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Title

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Description

Characteristics

GSM679415

GPL1352

Disc Cells in 3D Control (95)

Disc Cells in 3D Control

cell type: annulus disc cells tissue grade: IV feed media: MEM20

Sample_geo_accession	GSM679415
Sample_status	Public on Jun 27 2012
Sample_submission_date	Feb 24 2011
Sample_last_update_date	Oct 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_growth_protocol_ch1	Human annulus disc cells (100,000) were seeded into each piece (~0.5 cm3) of a collagen construct (Gelfoam; Pharmacia & Upjohn Co., Kalamazoo, MI). They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Each piece of Gelfoam was homongenized and RNA isolated using TRIzol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA were prepard according to the Affymetrix protocol for IVT labeling.
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL1352
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679415/suppl/GSM679415.CEL.gz
Sample_relation	Reanalyzed by: GSM1026926
Sample_series_id	GSE27494
Sample_data_row_count	61359

GSM679416

GPL1352

Disc Cells in 3D IL-1 (95)

Disc Cells in 3D IL-1

cell type: annulus disc cells tissue grade: IV feed media: MEM20 + 10 2 pM

Sample_geo_accession	GSM679416
Sample_status	Public on Jun 27 2012
Sample_submission_date	Feb 24 2011
Sample_last_update_date	Jun 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_growth_protocol_ch1	Human annulus disc cells (100,000) were seeded into each piece (~0.5 cm3) of a collagen construct (Gelfoam; Pharmacia & Upjohn Co., Kalamazoo, MI). They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Each piece of Gelfoam was homongenized and RNA isolated using TRIzol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA were prepard according to the Affymetrix protocol for IVT labeling.
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL1352
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679416/suppl/GSM679416.CEL.gz
Sample_series_id	GSE27494
Sample_data_row_count	61359

GSM679417

GPL1352

Disc Cells in 3D Control (107)

Disc Cells in 3D Control

cell type: annulus disc cells tissue grade: IV feed media: MEM20

Sample_geo_accession	GSM679417
Sample_status	Public on Jun 27 2012
Sample_submission_date	Feb 24 2011
Sample_last_update_date	Jun 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_growth_protocol_ch1	Human annulus disc cells (100,000) were seeded into each piece (~0.5 cm3) of a collagen construct (Gelfoam; Pharmacia & Upjohn Co., Kalamazoo, MI). They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Each piece of Gelfoam was homongenized and RNA isolated using TRIzol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA were prepard according to the Affymetrix protocol for IVT labeling.
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL1352
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679417/suppl/GSM679417.CEL.gz
Sample_series_id	GSE27494
Sample_data_row_count	61359

GSM679418

GPL1352

Disc Cells in 3D IL-1 (107)

Disc Cells in 3D IL-1

cell type: annulus disc cells tissue grade: IV feed media: MEM20 + 10 2 pM

Sample_geo_accession	GSM679418
Sample_status	Public on Jun 27 2012
Sample_submission_date	Feb 24 2011
Sample_last_update_date	Jun 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_growth_protocol_ch1	Human annulus disc cells (100,000) were seeded into each piece (~0.5 cm3) of a collagen construct (Gelfoam; Pharmacia & Upjohn Co., Kalamazoo, MI). They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Each piece of Gelfoam was homongenized and RNA isolated using TRIzol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA were prepard according to the Affymetrix protocol for IVT labeling.
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL1352
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679418/suppl/GSM679418.CEL.gz
Sample_series_id	GSE27494
Sample_data_row_count	61359

GSM679419

GPL1352

Disc Cells in 3D Control (126)

Disc Cells in 3D Control

cell type: annulus disc cells tissue grade: III feed media: MEM20

Sample_geo_accession	GSM679419
Sample_status	Public on Jun 27 2012
Sample_submission_date	Feb 24 2011
Sample_last_update_date	Oct 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_growth_protocol_ch1	Human annulus disc cells (100,000) were seeded into each piece (~0.5 cm3) of a collagen construct (Gelfoam; Pharmacia & Upjohn Co., Kalamazoo, MI). They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Each piece of Gelfoam was homongenized and RNA isolated using TRIzol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA were prepard according to the Affymetrix protocol for IVT labeling.
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL1352
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679419/suppl/GSM679419.CEL.gz
Sample_relation	Reanalyzed by: GSM1026928
Sample_series_id	GSE27494
Sample_data_row_count	61359

GSM679420

GPL1352

Disc Cells in 3D IL-1 (126)

Disc Cells in 3D IL-1

cell type: annulus disc cells tissue grade: III feed media: MEM20 + 10 2 pM

Sample_geo_accession	GSM679420
Sample_status	Public on Jun 27 2012
Sample_submission_date	Feb 24 2011
Sample_last_update_date	Jun 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_growth_protocol_ch1	Human annulus disc cells (100,000) were seeded into each piece (~0.5 cm3) of a collagen construct (Gelfoam; Pharmacia & Upjohn Co., Kalamazoo, MI). They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Each piece of Gelfoam was homongenized and RNA isolated using TRIzol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA were prepard according to the Affymetrix protocol for IVT labeling.
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL1352
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679420/suppl/GSM679420.CEL.gz
Sample_series_id	GSE27494
Sample_data_row_count	61359

GSM679421

GPL1352

Disc Cells in 3D Control (154)

Disc Cells in 3D Control

cell type: annulus disc cells tissue grade: IV feed media: MEM20

Sample_geo_accession	GSM679421
Sample_status	Public on Jun 27 2012
Sample_submission_date	Feb 24 2011
Sample_last_update_date	Oct 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_growth_protocol_ch1	Human annulus disc cells (100,000) were seeded into each piece (~0.5 cm3) of a collagen construct (Gelfoam; Pharmacia & Upjohn Co., Kalamazoo, MI). They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Each piece of Gelfoam was homongenized and RNA isolated using TRIzol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA were prepard according to the Affymetrix protocol for IVT labeling.
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL1352
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679421/suppl/GSM679421.CEL.gz
Sample_relation	Reanalyzed by: GSM1026930
Sample_series_id	GSE27494
Sample_data_row_count	61359

GSM679422

GPL1352

Disc Cells in 3D IL-1 (154)

Disc Cells in 3D IL-1

cell type: annulus disc cells tissue grade: IV feed media: MEM20 + 10 2 pM

Sample_geo_accession	GSM679422
Sample_status	Public on Jun 27 2012
Sample_submission_date	Feb 24 2011
Sample_last_update_date	Jun 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_growth_protocol_ch1	Human annulus disc cells (100,000) were seeded into each piece (~0.5 cm3) of a collagen construct (Gelfoam; Pharmacia & Upjohn Co., Kalamazoo, MI). They were fed 3 times per week for 9 days with minimal essential medium with 20% fetal bovine serum (MEM20) +/- 102 pM IL-1beta. Cells were allowed to grow an additional 5 days without changing the media.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Each piece of Gelfoam was homongenized and RNA isolated using TRIzol reagent
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA were prepard according to the Affymetrix protocol for IVT labeling.
Sample_hyb_protocol	Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned once via the Affymetrix 3000G scanner.
Sample_data_processing	The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used. Further normalization was acheived using GC-RMA (robust multiarray average) via GeneSifter analysis software (Viz Labs LLC, Seattle , WA; http://www.genesifter.net).
Sample_platform_id	GPL1352
Sample_contact_name	Helen,,Gruber
Sample_contact_email	Helen.Gruber@carolinashealthcare.org
Sample_contact_laboratory	Orthopaedic Research
Sample_contact_department	Orthopaedic Surgery
Sample_contact_institute	Carolinas HealthCare System
Sample_contact_address	1542 Graden Terrace
Sample_contact_city	Charlotte
Sample_contact_state	NC
Sample_contact_zip/postal_code	28203
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679422/suppl/GSM679422.CEL.gz
Sample_series_id	GSE27494
Sample_data_row_count	61359

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