Search results for the GEO ID: GSE27505  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM679511 | GPL570 |
|
16wk VZ/SVZ Tissue
|
Human fetal forebrain VZ/SVZ, 16 wk
|
tissue: forebrain ventricular and subventricular zone tissue
cell type: unsorted
developmental stage: fetal
gestational age: 16 weeks
|
108420-108399
Gene expression data from 16wk forebrain VZ/SVZ tissue.
|
| Sample_geo_accession | GSM679511
| | Sample_status | Public on Feb 25 2011
| | Sample_submission_date | Feb 24 2011
| | Sample_last_update_date | Feb 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Neural progenitor cells were infected with AdE/sox2:EGFP, at 5 multiplicities of infection (moi), for 8–12 h in DMEM/F12/N2 with 20 ng/ml bFGF. The infected cells were monitored daily until EGFP expression was detected, typically 3–5 d later, and were then dissociated with papain before FACS. Flow cytometry and sorting of E/sox2:EGFP and E/sox2:EGFP cells were performed on a FACSAria (Becton Dickinson).
| | Sample_growth_protocol_ch1 | Neural progenitor cells from human fetal VZ/SVZ tissues were dissociated and cultured in DMEM/F12/N2 containing 20 ng/ml bFGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy (Qiagen) and amplified using 3 -biased ribo-SPIA (NuGen Ovation), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | NuGEN Ovation Biotin System.
| | Sample_hyb_protocol | Standard Affymetrix protocol for Affymetrix U133 Plus 2.0 arrays.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Microarray analysis was performed in Genespring GX 7.3.1 (Agilent). Raw CEL data were processed using the MAS5 method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Steven,,Goldman
| | Sample_contact_email | Steven_Goldman@urmc.rochester.edu
| | Sample_contact_laboratory | Goldman Lab
| | Sample_contact_department | Center for Translational Neuromedicine
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Ave
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679511/suppl/GSM679511.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679511/suppl/GSM679511.CHP.gz
| | Sample_series_id | GSE27505
| | Sample_data_row_count | 54675
| | |
|
GSM679512 | GPL570 |
|
16wk SOX2-
|
Human fetal forebrain VZ/SVZ, SOX2-, 16 wk
|
tissue: forebrain ventricular and subventricular zone tissue
cell type: SOX2-
developmental stage: fetal
gestational age: 16 weeks
|
108424-108403
Gene expression data from SOX2- cells isolated from 16wk forebrain VZ/SVZ tissue.
|
| Sample_geo_accession | GSM679512
| | Sample_status | Public on Feb 25 2011
| | Sample_submission_date | Feb 24 2011
| | Sample_last_update_date | Feb 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Neural progenitor cells were infected with AdE/sox2:EGFP, at 5 multiplicities of infection (moi), for 8–12 h in DMEM/F12/N2 with 20 ng/ml bFGF. The infected cells were monitored daily until EGFP expression was detected, typically 3–5 d later, and were then dissociated with papain before FACS. Flow cytometry and sorting of E/sox2:EGFP and E/sox2:EGFP cells were performed on a FACSAria (Becton Dickinson).
| | Sample_growth_protocol_ch1 | Neural progenitor cells from human fetal VZ/SVZ tissues were dissociated and cultured in DMEM/F12/N2 containing 20 ng/ml bFGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy (Qiagen) and amplified using 3 -biased ribo-SPIA (NuGen Ovation), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | NuGEN Ovation Biotin System.
| | Sample_hyb_protocol | Standard Affymetrix protocol for Affymetrix U133 Plus 2.0 arrays.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Microarray analysis was performed in Genespring GX 7.3.1 (Agilent). Raw CEL data were processed using the MAS5 method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Steven,,Goldman
| | Sample_contact_email | Steven_Goldman@urmc.rochester.edu
| | Sample_contact_laboratory | Goldman Lab
| | Sample_contact_department | Center for Translational Neuromedicine
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Ave
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679512/suppl/GSM679512.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679512/suppl/GSM679512.CHP.gz
| | Sample_series_id | GSE27505
| | Sample_data_row_count | 54675
| | |
|
GSM679513 | GPL570 |
|
16wk SOX2+
|
Human fetal forebrain VZ/SVZ, SOX2+, 16 wk
|
tissue: forebrain ventricular and subventricular zone tissue
cell type: SOX2+
developmental stage: fetal
gestational age: 16 weeks
|
108426-108405
Gene expression data from SOX2+ cells isolated from 16wk forebrain VZ/SVZ tissue.
|
| Sample_geo_accession | GSM679513
| | Sample_status | Public on Feb 25 2011
| | Sample_submission_date | Feb 24 2011
| | Sample_last_update_date | Feb 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Neural progenitor cells were infected with AdE/sox2:EGFP, at 5 multiplicities of infection (moi), for 8–12 h in DMEM/F12/N2 with 20 ng/ml bFGF. The infected cells were monitored daily until EGFP expression was detected, typically 3–5 d later, and were then dissociated with papain before FACS. Flow cytometry and sorting of E/sox2:EGFP and E/sox2:EGFP cells were performed on a FACSAria (Becton Dickinson).
| | Sample_growth_protocol_ch1 | Neural progenitor cells from human fetal VZ/SVZ tissues were dissociated and cultured in DMEM/F12/N2 containing 20 ng/ml bFGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy (Qiagen) and amplified using 3 -biased ribo-SPIA (NuGen Ovation), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | NuGEN Ovation Biotin System.
| | Sample_hyb_protocol | Standard Affymetrix protocol for Affymetrix U133 Plus 2.0 arrays.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Microarray analysis was performed in Genespring GX 7.3.1 (Agilent). Raw CEL data were processed using the MAS5 method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Steven,,Goldman
| | Sample_contact_email | Steven_Goldman@urmc.rochester.edu
| | Sample_contact_laboratory | Goldman Lab
| | Sample_contact_department | Center for Translational Neuromedicine
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Ave
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679513/suppl/GSM679513.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679513/suppl/GSM679513.CHP.gz
| | Sample_series_id | GSE27505
| | Sample_data_row_count | 54675
| | |
|
GSM679514 | GPL570 |
|
17wk SOX2-
|
Human fetal forebrain VZ/SVZ, SOX2-, 17 wk
|
tissue: forebrain ventricular and subventricular zone tissue
cell type: SOX2-
developmental stage: fetal
gestational age: 17 weeks
|
108429-108408
Gene expression data from SOX2- cells isolated from 17wk forebrain VZ/SVZ tissue.
|
| Sample_geo_accession | GSM679514
| | Sample_status | Public on Feb 25 2011
| | Sample_submission_date | Feb 24 2011
| | Sample_last_update_date | Feb 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Neural progenitor cells were infected with AdE/sox2:EGFP, at 5 multiplicities of infection (moi), for 8–12 h in DMEM/F12/N2 with 20 ng/ml bFGF. The infected cells were monitored daily until EGFP expression was detected, typically 3–5 d later, and were then dissociated with papain before FACS. Flow cytometry and sorting of E/sox2:EGFP and E/sox2:EGFP cells were performed on a FACSAria (Becton Dickinson).
| | Sample_growth_protocol_ch1 | Neural progenitor cells from human fetal VZ/SVZ tissues were dissociated and cultured in DMEM/F12/N2 containing 20 ng/ml bFGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy (Qiagen) and amplified using 3 -biased ribo-SPIA (NuGen Ovation), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | NuGEN Ovation Biotin System.
| | Sample_hyb_protocol | Standard Affymetrix protocol for Affymetrix U133 Plus 2.0 arrays.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Microarray analysis was performed in Genespring GX 7.3.1 (Agilent). Raw CEL data were processed using the MAS5 method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Steven,,Goldman
| | Sample_contact_email | Steven_Goldman@urmc.rochester.edu
| | Sample_contact_laboratory | Goldman Lab
| | Sample_contact_department | Center for Translational Neuromedicine
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Ave
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679514/suppl/GSM679514.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679514/suppl/GSM679514.CHP.gz
| | Sample_series_id | GSE27505
| | Sample_data_row_count | 54675
| | |
|
GSM679515 | GPL570 |
|
17wk SOX2+
|
Human fetal forebrain VZ/SVZ, SOX2+, 17 wk
|
tissue: forebrain ventricular and subventricular zone tissue
cell type: SOX2+
developmental stage: fetal
gestational age: 17 weeks
|
108431-108410
Gene expression data from SOX2+ cells isolated from 17wk forebrain VZ/SVZ tissue.
|
| Sample_geo_accession | GSM679515
| | Sample_status | Public on Feb 25 2011
| | Sample_submission_date | Feb 24 2011
| | Sample_last_update_date | Feb 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Neural progenitor cells were infected with AdE/sox2:EGFP, at 5 multiplicities of infection (moi), for 8–12 h in DMEM/F12/N2 with 20 ng/ml bFGF. The infected cells were monitored daily until EGFP expression was detected, typically 3–5 d later, and were then dissociated with papain before FACS. Flow cytometry and sorting of E/sox2:EGFP and E/sox2:EGFP cells were performed on a FACSAria (Becton Dickinson).
| | Sample_growth_protocol_ch1 | Neural progenitor cells from human fetal VZ/SVZ tissues were dissociated and cultured in DMEM/F12/N2 containing 20 ng/ml bFGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy (Qiagen) and amplified using 3 -biased ribo-SPIA (NuGen Ovation), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | NuGEN Ovation Biotin System.
| | Sample_hyb_protocol | Standard Affymetrix protocol for Affymetrix U133 Plus 2.0 arrays.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Microarray analysis was performed in Genespring GX 7.3.1 (Agilent). Raw CEL data were processed using the MAS5 method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Steven,,Goldman
| | Sample_contact_email | Steven_Goldman@urmc.rochester.edu
| | Sample_contact_laboratory | Goldman Lab
| | Sample_contact_department | Center for Translational Neuromedicine
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Ave
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679515/suppl/GSM679515.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679515/suppl/GSM679515.CHP.gz
| | Sample_series_id | GSE27505
| | Sample_data_row_count | 54675
| | |
|
GSM679516 | GPL570 |
|
17wk VZ/SVZ Tissue
|
Human fetal forebrain VZ/SVZ, 17 wk
|
tissue: forebrain ventricular and subventricular zone tissue
cell type: unsorted
developmental stage: fetal
gestational age: 17 weeks
|
108433-108412
Gene expression data from 17wk forebrain VZ/SVZ tissue.
|
| Sample_geo_accession | GSM679516
| | Sample_status | Public on Feb 25 2011
| | Sample_submission_date | Feb 24 2011
| | Sample_last_update_date | Feb 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Neural progenitor cells were infected with AdE/sox2:EGFP, at 5 multiplicities of infection (moi), for 8–12 h in DMEM/F12/N2 with 20 ng/ml bFGF. The infected cells were monitored daily until EGFP expression was detected, typically 3–5 d later, and were then dissociated with papain before FACS. Flow cytometry and sorting of E/sox2:EGFP and E/sox2:EGFP cells were performed on a FACSAria (Becton Dickinson).
| | Sample_growth_protocol_ch1 | Neural progenitor cells from human fetal VZ/SVZ tissues were dissociated and cultured in DMEM/F12/N2 containing 20 ng/ml bFGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy (Qiagen) and amplified using 3 -biased ribo-SPIA (NuGen Ovation), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | NuGEN Ovation Biotin System.
| | Sample_hyb_protocol | Standard Affymetrix protocol for Affymetrix U133 Plus 2.0 arrays.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Microarray analysis was performed in Genespring GX 7.3.1 (Agilent). Raw CEL data were processed using the MAS5 method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Steven,,Goldman
| | Sample_contact_email | Steven_Goldman@urmc.rochester.edu
| | Sample_contact_laboratory | Goldman Lab
| | Sample_contact_department | Center for Translational Neuromedicine
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Ave
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679516/suppl/GSM679516.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679516/suppl/GSM679516.CHP.gz
| | Sample_series_id | GSE27505
| | Sample_data_row_count | 54675
| | |
|
GSM679517 | GPL570 |
|
19wk SOX2-
|
Human fetal forebrain VZ/SVZ, SOX2-, 19 wk
|
tissue: forebrain ventricular and subventricular zone tissue
cell type: SOX2-
developmental stage: fetal
gestational age: 19 weeks
|
108436-108415
Gene expression data from SOX2- cells isolated from 19wk forebrain VZ/SVZ tissue.
|
| Sample_geo_accession | GSM679517
| | Sample_status | Public on Feb 25 2011
| | Sample_submission_date | Feb 24 2011
| | Sample_last_update_date | Feb 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Neural progenitor cells were infected with AdE/sox2:EGFP, at 5 multiplicities of infection (moi), for 8–12 h in DMEM/F12/N2 with 20 ng/ml bFGF. The infected cells were monitored daily until EGFP expression was detected, typically 3–5 d later, and were then dissociated with papain before FACS. Flow cytometry and sorting of E/sox2:EGFP and E/sox2:EGFP cells were performed on a FACSAria (Becton Dickinson).
| | Sample_growth_protocol_ch1 | Neural progenitor cells from human fetal VZ/SVZ tissues were dissociated and cultured in DMEM/F12/N2 containing 20 ng/ml bFGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy (Qiagen) and amplified using 3 -biased ribo-SPIA (NuGen Ovation), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | NuGEN Ovation Biotin System.
| | Sample_hyb_protocol | Standard Affymetrix protocol for Affymetrix U133 Plus 2.0 arrays.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Microarray analysis was performed in Genespring GX 7.3.1 (Agilent). Raw CEL data were processed using the MAS5 method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Steven,,Goldman
| | Sample_contact_email | Steven_Goldman@urmc.rochester.edu
| | Sample_contact_laboratory | Goldman Lab
| | Sample_contact_department | Center for Translational Neuromedicine
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Ave
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679517/suppl/GSM679517.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679517/suppl/GSM679517.CHP.gz
| | Sample_series_id | GSE27505
| | Sample_data_row_count | 54675
| | |
|
GSM679518 | GPL570 |
|
19wk SOX2+
|
Human fetal forebrain VZ/SVZ, SOX2+, 19 wk
|
tissue: forebrain ventricular and subventricular zone tissue
cell type: SOX2+
developmental stage: fetal
gestational age: 19 weeks
|
108438-108417
Gene expression data from SOX2+ cells isolated from 19wk forebrain VZ/SVZ tissue.
|
| Sample_geo_accession | GSM679518
| | Sample_status | Public on Feb 25 2011
| | Sample_submission_date | Feb 24 2011
| | Sample_last_update_date | Feb 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Neural progenitor cells were infected with AdE/sox2:EGFP, at 5 multiplicities of infection (moi), for 8–12 h in DMEM/F12/N2 with 20 ng/ml bFGF. The infected cells were monitored daily until EGFP expression was detected, typically 3–5 d later, and were then dissociated with papain before FACS. Flow cytometry and sorting of E/sox2:EGFP and E/sox2:EGFP cells were performed on a FACSAria (Becton Dickinson).
| | Sample_growth_protocol_ch1 | Neural progenitor cells from human fetal VZ/SVZ tissues were dissociated and cultured in DMEM/F12/N2 containing 20 ng/ml bFGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy (Qiagen) and amplified using 3 -biased ribo-SPIA (NuGen Ovation), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | NuGEN Ovation Biotin System.
| | Sample_hyb_protocol | Standard Affymetrix protocol for Affymetrix U133 Plus 2.0 arrays.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Microarray analysis was performed in Genespring GX 7.3.1 (Agilent). Raw CEL data were processed using the MAS5 method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Steven,,Goldman
| | Sample_contact_email | Steven_Goldman@urmc.rochester.edu
| | Sample_contact_laboratory | Goldman Lab
| | Sample_contact_department | Center for Translational Neuromedicine
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Ave
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679518/suppl/GSM679518.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679518/suppl/GSM679518.CHP.gz
| | Sample_series_id | GSE27505
| | Sample_data_row_count | 54675
| | |
|
GSM679519 | GPL570 |
|
19wk VZ/SVZ Tissue
|
Human fetal forebrain VZ/SVZ, 19 wk
|
tissue: forebrain ventricular and subventricular zone tissue
cell type: unsorted
developmental stage: fetal
gestational age: 19 weeks
|
108439-108418
Gene expression data from 19wk forebrain VZ/SVZ tissue.
|
| Sample_geo_accession | GSM679519
| | Sample_status | Public on Feb 25 2011
| | Sample_submission_date | Feb 24 2011
| | Sample_last_update_date | Feb 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Neural progenitor cells were infected with AdE/sox2:EGFP, at 5 multiplicities of infection (moi), for 8–12 h in DMEM/F12/N2 with 20 ng/ml bFGF. The infected cells were monitored daily until EGFP expression was detected, typically 3–5 d later, and were then dissociated with papain before FACS. Flow cytometry and sorting of E/sox2:EGFP and E/sox2:EGFP cells were performed on a FACSAria (Becton Dickinson).
| | Sample_growth_protocol_ch1 | Neural progenitor cells from human fetal VZ/SVZ tissues were dissociated and cultured in DMEM/F12/N2 containing 20 ng/ml bFGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy (Qiagen) and amplified using 3 -biased ribo-SPIA (NuGen Ovation), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | NuGEN Ovation Biotin System.
| | Sample_hyb_protocol | Standard Affymetrix protocol for Affymetrix U133 Plus 2.0 arrays.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Microarray analysis was performed in Genespring GX 7.3.1 (Agilent). Raw CEL data were processed using the MAS5 method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Steven,,Goldman
| | Sample_contact_email | Steven_Goldman@urmc.rochester.edu
| | Sample_contact_laboratory | Goldman Lab
| | Sample_contact_department | Center for Translational Neuromedicine
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Ave
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679519/suppl/GSM679519.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679519/suppl/GSM679519.CHP.gz
| | Sample_series_id | GSE27505
| | Sample_data_row_count | 54675
| | |
|
GSM679520 | GPL570 |
|
18wk SOX2-
|
Human fetal forebrain VZ/SVZ, SOX2-, 18 wk
|
tissue: forebrain ventricular and subventricular zone tissue
cell type: SOX2-
developmental stage: fetal
gestational age: 18 weeks
|
108833-108822
Gene expression data from SOX2- cells isolated from 18wk forebrain VZ/SVZ tissue.
|
| Sample_geo_accession | GSM679520
| | Sample_status | Public on Feb 25 2011
| | Sample_submission_date | Feb 24 2011
| | Sample_last_update_date | Feb 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Neural progenitor cells were infected with AdE/sox2:EGFP, at 5 multiplicities of infection (moi), for 8–12 h in DMEM/F12/N2 with 20 ng/ml bFGF. The infected cells were monitored daily until EGFP expression was detected, typically 3–5 d later, and were then dissociated with papain before FACS. Flow cytometry and sorting of E/sox2:EGFP and E/sox2:EGFP cells were performed on a FACSAria (Becton Dickinson).
| | Sample_growth_protocol_ch1 | Neural progenitor cells from human fetal VZ/SVZ tissues were dissociated and cultured in DMEM/F12/N2 containing 20 ng/ml bFGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy (Qiagen) and amplified using 3 -biased ribo-SPIA (NuGen Ovation), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | NuGEN Ovation Biotin System.
| | Sample_hyb_protocol | Standard Affymetrix protocol for Affymetrix U133 Plus 2.0 arrays.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Microarray analysis was performed in Genespring GX 7.3.1 (Agilent). Raw CEL data were processed using the MAS5 method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Steven,,Goldman
| | Sample_contact_email | Steven_Goldman@urmc.rochester.edu
| | Sample_contact_laboratory | Goldman Lab
| | Sample_contact_department | Center for Translational Neuromedicine
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Ave
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679520/suppl/GSM679520.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679520/suppl/GSM679520.CHP.gz
| | Sample_series_id | GSE27505
| | Sample_data_row_count | 54675
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GSM679521 | GPL570 |
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18wk SOX2+
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Human fetal forebrain VZ/SVZ, SOX2+, 18 wk
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tissue: forebrain ventricular and subventricular zone tissue
cell type: SOX2+
developmental stage: fetal
gestational age: 18 weeks
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108834-108823
Gene expression data from SOX2+ cells isolated from 18wk forebrain VZ/SVZ tissue.
|
| Sample_geo_accession | GSM679521
| | Sample_status | Public on Feb 25 2011
| | Sample_submission_date | Feb 24 2011
| | Sample_last_update_date | Feb 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Neural progenitor cells were infected with AdE/sox2:EGFP, at 5 multiplicities of infection (moi), for 8–12 h in DMEM/F12/N2 with 20 ng/ml bFGF. The infected cells were monitored daily until EGFP expression was detected, typically 3–5 d later, and were then dissociated with papain before FACS. Flow cytometry and sorting of E/sox2:EGFP and E/sox2:EGFP cells were performed on a FACSAria (Becton Dickinson).
| | Sample_growth_protocol_ch1 | Neural progenitor cells from human fetal VZ/SVZ tissues were dissociated and cultured in DMEM/F12/N2 containing 20 ng/ml bFGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy (Qiagen) and amplified using 3 -biased ribo-SPIA (NuGen Ovation), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | NuGEN Ovation Biotin System.
| | Sample_hyb_protocol | Standard Affymetrix protocol for Affymetrix U133 Plus 2.0 arrays.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Microarray analysis was performed in Genespring GX 7.3.1 (Agilent). Raw CEL data were processed using the MAS5 method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Steven,,Goldman
| | Sample_contact_email | Steven_Goldman@urmc.rochester.edu
| | Sample_contact_laboratory | Goldman Lab
| | Sample_contact_department | Center for Translational Neuromedicine
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Ave
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679521/suppl/GSM679521.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679521/suppl/GSM679521.CHP.gz
| | Sample_series_id | GSE27505
| | Sample_data_row_count | 54675
| | |
|
GSM679522 | GPL570 |
|
18wk VZ/SVZ Tissue
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Human fetal forebrain VZ/SVZ, 18 wk
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tissue: forebrain ventricular and subventricular zone tissue
cell type: unsorted
developmental stage: fetal
gestational age: 18 weeks
|
108835-108824
Gene expression data from 18wk forebrain VZ/SVZ tissue.
|
| Sample_geo_accession | GSM679522
| | Sample_status | Public on Feb 25 2011
| | Sample_submission_date | Feb 24 2011
| | Sample_last_update_date | Feb 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Neural progenitor cells were infected with AdE/sox2:EGFP, at 5 multiplicities of infection (moi), for 8–12 h in DMEM/F12/N2 with 20 ng/ml bFGF. The infected cells were monitored daily until EGFP expression was detected, typically 3–5 d later, and were then dissociated with papain before FACS. Flow cytometry and sorting of E/sox2:EGFP and E/sox2:EGFP cells were performed on a FACSAria (Becton Dickinson).
| | Sample_growth_protocol_ch1 | Neural progenitor cells from human fetal VZ/SVZ tissues were dissociated and cultured in DMEM/F12/N2 containing 20 ng/ml bFGF.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy (Qiagen) and amplified using 3 -biased ribo-SPIA (NuGen Ovation), according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | NuGEN Ovation Biotin System.
| | Sample_hyb_protocol | Standard Affymetrix protocol for Affymetrix U133 Plus 2.0 arrays.
| | Sample_scan_protocol | Standard Affymetrix protocol.
| | Sample_data_processing | Microarray analysis was performed in Genespring GX 7.3.1 (Agilent). Raw CEL data were processed using the MAS5 method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Steven,,Goldman
| | Sample_contact_email | Steven_Goldman@urmc.rochester.edu
| | Sample_contact_laboratory | Goldman Lab
| | Sample_contact_department | Center for Translational Neuromedicine
| | Sample_contact_institute | University of Rochester
| | Sample_contact_address | 601 Elmwood Ave
| | Sample_contact_city | Rochester
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 14642
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679522/suppl/GSM679522.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679522/suppl/GSM679522.CHP.gz
| | Sample_series_id | GSE27505
| | Sample_data_row_count | 54675
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