Search results for the GEO ID: GSE27529  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM679909 | GPL570 |
|
miR9.9h.1
|
L428 miR-9 LNA
|
cell line: L428
treatment: LNA inhibitor
|
|
| Sample_geo_accession | GSM679909
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Feb 25 2011
| | Sample_last_update_date | May 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Transfected by nucleofection using solution L and program X-001.
| | Sample_growth_protocol_ch1 | L428 cells were cultured in RPMI 1640 GlutaMAX medium (Gibco, Invitrogen) supplemented with 10% (v/v) fetal bovine serum (Hyclone, Thermo Scientific) and incubated in 5% CO2 plus 20% oxygen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with TRIZOL.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cDNA was used as template for an “in vitro” transcription reaction generating biotin-labeled antisense cRNA (BioArrayTM High Yield RNA Transcript Labeling kit; Enzo Diagnostics, Farmingdale, NY).
| | Sample_hyb_protocol | After fragmentation at 94 °C for 35 min in 40 mM Tris, 30 mM MgOAc, 10 mM KOAc, samples were hybridized for 16 h to Affymetrix HG-U133 2.0 human arrays (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | The arrays were washed and stained with phycoerythrin-conjugated streptavidin (SAPE), and the arrays were scanned in the Affymetrix GeneArray 2500 scanner, exactly as described in the Affymetrix GeneChip protocol.
| | Sample_data_processing | The expression data was processed using the “affy” package in BioConductor in R. Probe set intensities were summarized using the Robust Multichip Average method and then transformed to generalized log values with the variance stable VSN method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lea,Haarup,Gregersen
| | Sample_contact_email | lea.gregersen@mdc-berlin.de
| | Sample_contact_phone | +493094063528
| | Sample_contact_institute | Max-Delbrück-Centrum für Molekulare Medizin (MDC)
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13092
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679909/suppl/GSM679909.CEL.gz
| | Sample_series_id | GSE27529
| | Sample_data_row_count | 54675
| | |
|
GSM679910 | GPL570 |
|
miR9.9h.2
|
L428 miR-9 LNA
|
cell line: L428
treatment: LNA inhibitor
|
|
| Sample_geo_accession | GSM679910
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Feb 25 2011
| | Sample_last_update_date | May 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Transfected by nucleofection using solution L and program X-001.
| | Sample_growth_protocol_ch1 | L428 cells were cultured in RPMI 1640 GlutaMAX medium (Gibco, Invitrogen) supplemented with 10% (v/v) fetal bovine serum (Hyclone, Thermo Scientific) and incubated in 5% CO2 plus 20% oxygen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with TRIZOL.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cDNA was used as template for an “in vitro” transcription reaction generating biotin-labeled antisense cRNA (BioArrayTM High Yield RNA Transcript Labeling kit; Enzo Diagnostics, Farmingdale, NY).
| | Sample_hyb_protocol | After fragmentation at 94 °C for 35 min in 40 mM Tris, 30 mM MgOAc, 10 mM KOAc, samples were hybridized for 16 h to Affymetrix HG-U133 2.0 human arrays (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | The arrays were washed and stained with phycoerythrin-conjugated streptavidin (SAPE), and the arrays were scanned in the Affymetrix GeneArray 2500 scanner, exactly as described in the Affymetrix GeneChip protocol.
| | Sample_data_processing | The expression data was processed using the “affy” package in BioConductor in R. Probe set intensities were summarized using the Robust Multichip Average method and then transformed to generalized log values with the variance stable VSN method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lea,Haarup,Gregersen
| | Sample_contact_email | lea.gregersen@mdc-berlin.de
| | Sample_contact_phone | +493094063528
| | Sample_contact_institute | Max-Delbrück-Centrum für Molekulare Medizin (MDC)
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13092
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679910/suppl/GSM679910.CEL.gz
| | Sample_series_id | GSE27529
| | Sample_data_row_count | 54675
| | |
|
GSM679911 | GPL570 |
|
miR9.9h.3
|
L428 miR-9 LNA
|
cell line: L428
treatment: LNA inhibitor
|
|
| Sample_geo_accession | GSM679911
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Feb 25 2011
| | Sample_last_update_date | May 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Transfected by nucleofection using solution L and program X-001.
| | Sample_growth_protocol_ch1 | L428 cells were cultured in RPMI 1640 GlutaMAX medium (Gibco, Invitrogen) supplemented with 10% (v/v) fetal bovine serum (Hyclone, Thermo Scientific) and incubated in 5% CO2 plus 20% oxygen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with TRIZOL.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cDNA was used as template for an “in vitro” transcription reaction generating biotin-labeled antisense cRNA (BioArrayTM High Yield RNA Transcript Labeling kit; Enzo Diagnostics, Farmingdale, NY).
| | Sample_hyb_protocol | After fragmentation at 94 °C for 35 min in 40 mM Tris, 30 mM MgOAc, 10 mM KOAc, samples were hybridized for 16 h to Affymetrix HG-U133 2.0 human arrays (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | The arrays were washed and stained with phycoerythrin-conjugated streptavidin (SAPE), and the arrays were scanned in the Affymetrix GeneArray 2500 scanner, exactly as described in the Affymetrix GeneChip protocol.
| | Sample_data_processing | The expression data was processed using the “affy” package in BioConductor in R. Probe set intensities were summarized using the Robust Multichip Average method and then transformed to generalized log values with the variance stable VSN method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lea,Haarup,Gregersen
| | Sample_contact_email | lea.gregersen@mdc-berlin.de
| | Sample_contact_phone | +493094063528
| | Sample_contact_institute | Max-Delbrück-Centrum für Molekulare Medizin (MDC)
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13092
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679911/suppl/GSM679911.CEL.gz
| | Sample_series_id | GSE27529
| | Sample_data_row_count | 54675
| | |
|
GSM679912 | GPL570 |
|
SCR.9h.1
|
L428 scramble LNA
|
cell line: L428
treatment: scrambled LNA
|
|
| Sample_geo_accession | GSM679912
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Feb 25 2011
| | Sample_last_update_date | May 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Transfected by nucleofection using solution L and program X-001.
| | Sample_growth_protocol_ch1 | L428 cells were cultured in RPMI 1640 GlutaMAX medium (Gibco, Invitrogen) supplemented with 10% (v/v) fetal bovine serum (Hyclone, Thermo Scientific) and incubated in 5% CO2 plus 20% oxygen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with TRIZOL.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cDNA was used as template for an “in vitro” transcription reaction generating biotin-labeled antisense cRNA (BioArrayTM High Yield RNA Transcript Labeling kit; Enzo Diagnostics, Farmingdale, NY).
| | Sample_hyb_protocol | After fragmentation at 94 °C for 35 min in 40 mM Tris, 30 mM MgOAc, 10 mM KOAc, samples were hybridized for 16 h to Affymetrix HG-U133 2.0 human arrays (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | The arrays were washed and stained with phycoerythrin-conjugated streptavidin (SAPE), and the arrays were scanned in the Affymetrix GeneArray 2500 scanner, exactly as described in the Affymetrix GeneChip protocol.
| | Sample_data_processing | The expression data was processed using the “affy” package in BioConductor in R. Probe set intensities were summarized using the Robust Multichip Average method and then transformed to generalized log values with the variance stable VSN method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lea,Haarup,Gregersen
| | Sample_contact_email | lea.gregersen@mdc-berlin.de
| | Sample_contact_phone | +493094063528
| | Sample_contact_institute | Max-Delbrück-Centrum für Molekulare Medizin (MDC)
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13092
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679912/suppl/GSM679912.CEL.gz
| | Sample_series_id | GSE27529
| | Sample_data_row_count | 54675
| | |
|
GSM679913 | GPL570 |
|
SCR.9h.2
|
L428 scramble LNA
|
cell line: L428
treatment: scrambled LNA
|
|
| Sample_geo_accession | GSM679913
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Feb 25 2011
| | Sample_last_update_date | May 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Transfected by nucleofection using solution L and program X-001.
| | Sample_growth_protocol_ch1 | L428 cells were cultured in RPMI 1640 GlutaMAX medium (Gibco, Invitrogen) supplemented with 10% (v/v) fetal bovine serum (Hyclone, Thermo Scientific) and incubated in 5% CO2 plus 20% oxygen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with TRIZOL.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cDNA was used as template for an “in vitro” transcription reaction generating biotin-labeled antisense cRNA (BioArrayTM High Yield RNA Transcript Labeling kit; Enzo Diagnostics, Farmingdale, NY).
| | Sample_hyb_protocol | After fragmentation at 94 °C for 35 min in 40 mM Tris, 30 mM MgOAc, 10 mM KOAc, samples were hybridized for 16 h to Affymetrix HG-U133 2.0 human arrays (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | The arrays were washed and stained with phycoerythrin-conjugated streptavidin (SAPE), and the arrays were scanned in the Affymetrix GeneArray 2500 scanner, exactly as described in the Affymetrix GeneChip protocol.
| | Sample_data_processing | The expression data was processed using the “affy” package in BioConductor in R. Probe set intensities were summarized using the Robust Multichip Average method and then transformed to generalized log values with the variance stable VSN method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lea,Haarup,Gregersen
| | Sample_contact_email | lea.gregersen@mdc-berlin.de
| | Sample_contact_phone | +493094063528
| | Sample_contact_institute | Max-Delbrück-Centrum für Molekulare Medizin (MDC)
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13092
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679913/suppl/GSM679913.CEL.gz
| | Sample_series_id | GSE27529
| | Sample_data_row_count | 54675
| | |
|
GSM679914 | GPL570 |
|
SCR.9h.3
|
L428 scramble LNA
|
cell line: L428
treatment: scrambled LNA
|
|
| Sample_geo_accession | GSM679914
| | Sample_status | Public on May 02 2012
| | Sample_submission_date | Feb 25 2011
| | Sample_last_update_date | May 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Transfected by nucleofection using solution L and program X-001.
| | Sample_growth_protocol_ch1 | L428 cells were cultured in RPMI 1640 GlutaMAX medium (Gibco, Invitrogen) supplemented with 10% (v/v) fetal bovine serum (Hyclone, Thermo Scientific) and incubated in 5% CO2 plus 20% oxygen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with TRIZOL.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total cDNA was used as template for an “in vitro” transcription reaction generating biotin-labeled antisense cRNA (BioArrayTM High Yield RNA Transcript Labeling kit; Enzo Diagnostics, Farmingdale, NY).
| | Sample_hyb_protocol | After fragmentation at 94 °C for 35 min in 40 mM Tris, 30 mM MgOAc, 10 mM KOAc, samples were hybridized for 16 h to Affymetrix HG-U133 2.0 human arrays (Affymetrix, Santa Clara, CA).
| | Sample_scan_protocol | The arrays were washed and stained with phycoerythrin-conjugated streptavidin (SAPE), and the arrays were scanned in the Affymetrix GeneArray 2500 scanner, exactly as described in the Affymetrix GeneChip protocol.
| | Sample_data_processing | The expression data was processed using the “affy” package in BioConductor in R. Probe set intensities were summarized using the Robust Multichip Average method and then transformed to generalized log values with the variance stable VSN method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lea,Haarup,Gregersen
| | Sample_contact_email | lea.gregersen@mdc-berlin.de
| | Sample_contact_phone | +493094063528
| | Sample_contact_institute | Max-Delbrück-Centrum für Molekulare Medizin (MDC)
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13092
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM679nnn/GSM679914/suppl/GSM679914.CEL.gz
| | Sample_series_id | GSE27529
| | Sample_data_row_count | 54675
| | |
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