Home   Search   Browse   Statistics   User guide   FAQs   Post question   Download   Process data  
Search results for the GEO ID: GSE27562
(Click on the check boxes provided under "Select for analysis", to initiate grouping)
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down)
GSM ID
GPL ID
Select for analysis
Title
Source name
Description
Characteristics
GSM681982
GPL570
PBMC_normal_training_1 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681983
GPL570
PBMC_normal_training_2 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681984
GPL570
PBMC_normal_training_3 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681985
GPL570
PBMC_normal_training_4 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681986
GPL570
PBMC_normal_training_5 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681987
GPL570
PBMC_normal_training_6 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681988
GPL570
PBMC_normal_training_7 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681989
GPL570
PBMC_normal_training_8 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681990
GPL570
PBMC_normal_training_9 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681991
GPL570
PBMC_normal_training_10 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681992
GPL570
PBMC_malignant_training_1 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681993
GPL570
PBMC_malignant_training_2 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681994
GPL570
PBMC_malignant_training_3 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681995
GPL570
PBMC_malignant_training_4 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681996
GPL570
PBMC_malignant_training_5 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681997
GPL570
PBMC_malignant_training_6 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681998
GPL570
PBMC_malignant_training_7 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM681999
GPL570
PBMC_malignant_training_8 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682000
GPL570
PBMC_malignant_training_9 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682001
GPL570
PBMC_malignant_training_10 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: training Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682002
GPL570
PBMC_benign_training_1 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682003
GPL570
PBMC_benign_training_2 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682004
GPL570
PBMC_benign_training_3 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682005
GPL570
PBMC_benign_training_4 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682006
GPL570
PBMC_benign_training_5 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682007
GPL570
PBMC_benign_training_6 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682008
GPL570
PBMC_benign_training_7 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682009
GPL570
PBMC_benign_training_8 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682010
GPL570
PBMC_benign_training_9 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682011
GPL570
PBMC_benign_training_10 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682012
GPL570
PBMC_ectopic_validation_1 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682013
GPL570
PBMC_ectopic_validation_2 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682014
GPL570
PBMC_ectopic_validation_3 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682015
GPL570
PBMC_ectopic_validation_4 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682016
GPL570
PBMC_ectopic_validation_5 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682017
GPL570
PBMC_ectopic_validation_6 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682018
GPL570
PBMC_ectopic_validation_7 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682019
GPL570
PBMC_ectopic_validation_8 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682020
GPL570
PBMC_ectopic_validation_9 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682021
GPL570
PBMC_ectopic_validation_10 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682022
GPL570
PBMC_ectopic_validation_11 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682023
GPL570
PBMC_ectopic_validation_12 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682024
GPL570
PBMC_ectopic_validation_13 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682025
GPL570
PBMC_ectopic_validation_14 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682026
GPL570
PBMC_ectopic_validation_15 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682027
GPL570
PBMC_ectopic_validation_16 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood leukocytes data set: validation Blood collection: Samples collected by leukapheresis and stored in liquid nitrogen. PBMC isolation:
GSM682028
GPL570
PBMC_ectopic_validation_17 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood leukocytes data set: validation Blood collection: Samples collected by leukapheresis and stored in liquid nitrogen. PBMC isolation:
GSM682029
GPL570
PBMC_ectopic_validation_18 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood leukocytes data set: validation Blood collection: Samples collected by leukapheresis and stored in liquid nitrogen. PBMC isolation:
GSM682030
GPL570
PBMC_ectopic_validation_19 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood leukocytes data set: validation Blood collection: Samples collected by leukapheresis and stored in liquid nitrogen. PBMC isolation:
GSM682031
GPL570
PBMC_ectopic_validation_20 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood leukocytes data set: validation Blood collection: Samples collected by leukapheresis and stored in liquid nitrogen. PBMC isolation:
GSM682032
GPL570
PBMC_ectopic_validation_21 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood leukocytes data set: validation Blood collection: Samples collected by leukapheresis and stored in liquid nitrogen. PBMC isolation:
GSM682033
GPL570
PBMC_ectopic_validation_22 PBMCs from patients with other cancer types (e.g., gastrointestinal and brain cancers) phenotype: Ectopic tissue: peripheral blood leukocytes data set: validation Blood collection: Samples collected by leukapheresis and stored in liquid nitrogen. PBMC isolation:
GSM682034
GPL570
PBMC_normal_validation_1 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682035
GPL570
PBMC_normal_validation_2 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682036
GPL570
PBMC_normal_validation_3 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682037
GPL570
PBMC_normal_validation_4 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682038
GPL570
PBMC_normal_validation_5 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682039
GPL570
PBMC_normal_validation_6 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682040
GPL570
PBMC_normal_validation_7 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682041
GPL570
PBMC_normal_validation_8 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682042
GPL570
PBMC_normal_validation_9 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682043
GPL570
PBMC_normal_validation_10 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682044
GPL570
PBMC_normal_validation_11 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682045
GPL570
PBMC_normal_validation_12 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682046
GPL570
PBMC_normal_validation_13 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682047
GPL570
PBMC_normal_validation_14 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682048
GPL570
PBMC_normal_validation_15 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682049
GPL570
PBMC_normal_validation_16 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682050
GPL570
PBMC_normal_validation_17 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682051
GPL570
PBMC_normal_validation_18 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682052
GPL570
PBMC_normal_validation_19 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682054
GPL570
PBMC_normal_validation_21 PBMCs from patients with normal mammogram phenotype: Normal tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682055
GPL570
PBMC_malignant_validation_1 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682056
GPL570
PBMC_malignant_validation_2 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682057
GPL570
PBMC_malignant_validation_3 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682058
GPL570
PBMC_malignant_validation_4 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682059
GPL570
PBMC_malignant_validation_5 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682060
GPL570
PBMC_malignant_validation_6 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682061
GPL570
PBMC_malignant_validation_7 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682062
GPL570
PBMC_malignant_validation_8 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682063
GPL570
PBMC_malignant_validation_9 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682064
GPL570
PBMC_malignant_validation_10 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682065
GPL570
PBMC_malignant_validation_11 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682066
GPL570
PBMC_malignant_validation_12 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682067
GPL570
PBMC_malignant_validation_13 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682068
GPL570
PBMC_malignant_validation_14 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682069
GPL570
PBMC_malignant_validation_15 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682070
GPL570
PBMC_malignant_validation_16 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682071
GPL570
PBMC_malignant_validation_17 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682072
GPL570
PBMC_malignant_validation_18 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682073
GPL570
PBMC_malignant_validation_19 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682074
GPL570
PBMC_malignant_validation_20 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682075
GPL570
PBMC_malignant_validation_21 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682076
GPL570
PBMC_malignant_validation_22 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682077
GPL570
PBMC_malignant_validation_23 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682078
GPL570
PBMC_malignant_validation_24 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682079
GPL570
PBMC_malignant_validation_25 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682080
GPL570
PBMC_malignant_validation_26 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682081
GPL570
PBMC_malignant_validation_27 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682082
GPL570
PBMC_malignant_validation_28 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682083
GPL570
PBMC_malignant_validation_29 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682084
GPL570
PBMC_malignant_validation_30 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682085
GPL570
PBMC_malignant_validation_31 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682086
GPL570
PBMC_malignant_validation_32 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682087
GPL570
PBMC_malignant_validation_33 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682088
GPL570
PBMC_malignant_validation_34 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682089
GPL570
PBMC_malignant_validation_35 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682090
GPL570
PBMC_malignant_validation_36 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682091
GPL570
PBMC_malignant_validation_37 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682092
GPL570
PBMC_malignant_validation_38 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682093
GPL570
PBMC_malignant_validation_39 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682094
GPL570
PBMC_malignant_validation_40 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682095
GPL570
PBMC_malignant_validation_41 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Malignant tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682096
GPL570
PBMC_benign_validation_1 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682097
GPL570
PBMC_benign_validation_2 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682098
GPL570
PBMC_benign_validation_3 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682099
GPL570
PBMC_benign_validation_4 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682100
GPL570
PBMC_benign_validation_5 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682101
GPL570
PBMC_benign_validation_6 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682102
GPL570
PBMC_benign_validation_7 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682103
GPL570
PBMC_benign_validation_8 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682104
GPL570
PBMC_benign_validation_9 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682105
GPL570
PBMC_benign_validation_10 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682106
GPL570
PBMC_benign_validation_11 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682107
GPL570
PBMC_benign_validation_12 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682108
GPL570
PBMC_benign_validation_13 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682109
GPL570
PBMC_benign_validation_14 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682110
GPL570
PBMC_benign_validation_15 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682111
GPL570
PBMC_benign_validation_16 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682112
GPL570
PBMC_benign_validation_17 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682113
GPL570
PBMC_benign_validation_18 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682114
GPL570
PBMC_benign_validation_19 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682115
GPL570
PBMC_benign_validation_20 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682116
GPL570
PBMC_benign_validation_21 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682117
GPL570
PBMC_benign_validation_22 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682118
GPL570
PBMC_benign_validation_23 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682119
GPL570
PBMC_benign_validation_24 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682120
GPL570
PBMC_benign_validation_25 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682121
GPL570
PBMC_benign_validation_26 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682122
GPL570
PBMC_benign_validation_27 PBMCs from patients with benign diagnosis, confirmed by diagnostic biopsy phenotype: Benign tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682123
GPL570
PBMC_malignant_validation_42 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Pre-Surgery (aka Malignant) tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682124
GPL570
PBMC_malignant_validation_43 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Pre-Surgery (aka Malignant) tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682125
GPL570
PBMC_malignant_validation_44 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Pre-Surgery (aka Malignant) tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682126
GPL570
PBMC_malignant_validation_45 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Pre-Surgery (aka Malignant) tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682127
GPL570
PBMC_malignant_validation_46 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Pre-Surgery (aka Malignant) tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682128
GPL570
PBMC_malignant_validation_47 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy phenotype: Pre-Surgery (aka Malignant) tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682129
GPL570
PBMC_post-surgery_validation_1 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682130
GPL570
PBMC_post-surgery_validation_2 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682131
GPL570
PBMC_post-surgery_validation_3 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682132
GPL570
PBMC_post-surgery_validation_4 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682133
GPL570
PBMC_post-surgery_validation_5 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682134
GPL570
PBMC_post-surgery_validation_6 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682135
GPL570
PBMC_post-surgery_validation_7 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682136
GPL570
PBMC_post-surgery_validation_8 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682137
GPL570
PBMC_post-surgery_validation_9 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682138
GPL570
PBMC_post-surgery_validation_10 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682139
GPL570
PBMC_post-surgery_validation_11 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682140
GPL570
PBMC_post-surgery_validation_12 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682141
GPL570
PBMC_post-surgery_validation_13 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682142
GPL570
PBMC_post-surgery_validation_14 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
GSM682143
GPL570
PBMC_post-surgery_validation_15 PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy; sample taken following surgical excision of tumor. phenotype: Post-Surgery tissue: peripheral blood mononuclear cells data set: validation Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection. PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
 
 
Make groups for comparisons
(2 groups will be compared at a time)
Select GSMs and click on "Add groups"
Enter the group name here:


Select expression type
Transcripts profile based on;
A. Differential status (Up/Down regulation)
B. Absolute calls (Transcribed/Not-detected)
 
Filter results by number of probes