Search results for the GEO ID: GSE27631  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM684735 | GPL570 |
|
HUVEC_0h
|
HUVECs without BMP stimulation
|
tissue: human umbilical vein endothelial cells (Lonza)
treatment: none
|
|
| Sample_geo_accession | GSM684735
| | Sample_status | Public on Jul 16 2011
| | Sample_submission_date | Mar 02 2011
| | Sample_last_update_date | Jul 17 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were starved overnight and stimulated with either 50 ng/ml of BMP-6 or 1 ng/ml of BMP-9 for the indicated times.
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM-2 medium (Lonza). Cells were grown in a humidified incubator with 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using RNeasy mini kit (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Double-stranded cDNA was synthesized with the SuperScript Choice system (GIBCO) and a T7-(dT) 24 Primer (GE Amersham) from the total RNA. In vitro transcription was performed to produce biotin-labeled cRNA by using a BioArray HighYield RNA Transcript Labeling Kit (Affymetrix) according to the manufacturer’s instructions (Ishii et al, Genomics 68:136-143, 2000).
| | Sample_hyb_protocol | Array hybridization was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
| | Sample_scan_protocol | Array scanning was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
| | Sample_data_processing | Microarray Suite software 5.0 of GCOS (Affymetrix) was used with target intensity of 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daizo,,Koinuma
| | Sample_contact_email | d-koinuma@umin.ac.jp
| | Sample_contact_department | Molecular Pathology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1, Bunkyo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM684nnn/GSM684735/suppl/GSM684735.CEL.gz
| | Sample_series_id | GSE27631
| | Sample_series_id | GSE27661
| | Sample_data_row_count | 54675
| | |
|
GSM684736 | GPL570 |
|
HUVEC_BMP6_2h
|
HUVECs stimulated with BMP-6 for 2 h
|
tissue: human umbilical vein endothelial cells (Lonza)
treatment: stimulated with 50 ng/ml of BMP-6 for 2 h
|
|
| Sample_geo_accession | GSM684736
| | Sample_status | Public on Jul 16 2011
| | Sample_submission_date | Mar 02 2011
| | Sample_last_update_date | Jul 17 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were starved overnight and stimulated with either 50 ng/ml of BMP-6 or 1 ng/ml of BMP-9 for the indicated times.
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM-2 medium (Lonza). Cells were grown in a humidified incubator with 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using RNeasy mini kit (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Double-stranded cDNA was synthesized with the SuperScript Choice system (GIBCO) and a T7-(dT) 24 Primer (GE Amersham) from the total RNA. In vitro transcription was performed to produce biotin-labeled cRNA by using a BioArray HighYield RNA Transcript Labeling Kit (Affymetrix) according to the manufacturer’s instructions (Ishii et al, Genomics 68:136-143, 2000).
| | Sample_hyb_protocol | Array hybridization was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
| | Sample_scan_protocol | Array scanning was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
| | Sample_data_processing | Microarray Suite software 5.0 of GCOS (Affymetrix) was used with target intensity of 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daizo,,Koinuma
| | Sample_contact_email | d-koinuma@umin.ac.jp
| | Sample_contact_department | Molecular Pathology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1, Bunkyo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM684nnn/GSM684736/suppl/GSM684736.CEL.gz
| | Sample_series_id | GSE27631
| | Sample_series_id | GSE27661
| | Sample_data_row_count | 54675
| | |
|
GSM684737 | GPL570 |
|
HUVEC_BMP6_24h
|
HUVECs stimulated with BMP-6 for 24 h
|
tissue: human umbilical vein endothelial cells (Lonza)
treatment: stimulated with 50 ng/ml of BMP-6 for 24 h
|
|
| Sample_geo_accession | GSM684737
| | Sample_status | Public on Jul 16 2011
| | Sample_submission_date | Mar 02 2011
| | Sample_last_update_date | Jul 17 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were starved overnight and stimulated with either 50 ng/ml of BMP-6 or 1 ng/ml of BMP-9 for the indicated times.
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM-2 medium (Lonza). Cells were grown in a humidified incubator with 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using RNeasy mini kit (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Double-stranded cDNA was synthesized with the SuperScript Choice system (GIBCO) and a T7-(dT) 24 Primer (GE Amersham) from the total RNA. In vitro transcription was performed to produce biotin-labeled cRNA by using a BioArray HighYield RNA Transcript Labeling Kit (Affymetrix) according to the manufacturer’s instructions (Ishii et al, Genomics 68:136-143, 2000).
| | Sample_hyb_protocol | Array hybridization was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
| | Sample_scan_protocol | Array scanning was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
| | Sample_data_processing | Microarray Suite software 5.0 of GCOS (Affymetrix) was used with target intensity of 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daizo,,Koinuma
| | Sample_contact_email | d-koinuma@umin.ac.jp
| | Sample_contact_department | Molecular Pathology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1, Bunkyo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM684nnn/GSM684737/suppl/GSM684737.CEL.gz
| | Sample_series_id | GSE27631
| | Sample_series_id | GSE27661
| | Sample_data_row_count | 54675
| | |
|
GSM684738 | GPL570 |
|
HUVEC_BMP9_2h
|
HUVECs stimulated with BMP-9 for 2 h
|
tissue: human umbilical vein endothelial cells (Lonza)
treatment: stimulated with 1 ng/ml of BMP-9 for 2 h
|
|
| Sample_geo_accession | GSM684738
| | Sample_status | Public on Jul 16 2011
| | Sample_submission_date | Mar 02 2011
| | Sample_last_update_date | Jul 17 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were starved overnight and stimulated with either 50 ng/ml of BMP-6 or 1 ng/ml of BMP-9 for the indicated times.
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM-2 medium (Lonza). Cells were grown in a humidified incubator with 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using RNeasy mini kit (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Double-stranded cDNA was synthesized with the SuperScript Choice system (GIBCO) and a T7-(dT) 24 Primer (GE Amersham) from the total RNA. In vitro transcription was performed to produce biotin-labeled cRNA by using a BioArray HighYield RNA Transcript Labeling Kit (Affymetrix) according to the manufacturer’s instructions (Ishii et al, Genomics 68:136-143, 2000).
| | Sample_hyb_protocol | Array hybridization was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
| | Sample_scan_protocol | Array scanning was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
| | Sample_data_processing | Microarray Suite software 5.0 of GCOS (Affymetrix) was used with target intensity of 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daizo,,Koinuma
| | Sample_contact_email | d-koinuma@umin.ac.jp
| | Sample_contact_department | Molecular Pathology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1, Bunkyo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM684nnn/GSM684738/suppl/GSM684738.CEL.gz
| | Sample_series_id | GSE27631
| | Sample_series_id | GSE27661
| | Sample_data_row_count | 54675
| | |
|
GSM684739 | GPL570 |
|
HUVEC_BMP9_24h
|
HUVECs stimulated with BMP-9 for 24 h
|
tissue: human umbilical vein endothelial cells (Lonza)
treatment: stimulated with 1 ng/ml of BMP-9 for 24 h
|
|
| Sample_geo_accession | GSM684739
| | Sample_status | Public on Jul 16 2011
| | Sample_submission_date | Mar 02 2011
| | Sample_last_update_date | Jul 17 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were starved overnight and stimulated with either 50 ng/ml of BMP-6 or 1 ng/ml of BMP-9 for the indicated times.
| | Sample_growth_protocol_ch1 | HUVECs were maintained in EGM-2 medium (Lonza). Cells were grown in a humidified incubator with 5% CO2 at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were extracted using RNeasy mini kit (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Double-stranded cDNA was synthesized with the SuperScript Choice system (GIBCO) and a T7-(dT) 24 Primer (GE Amersham) from the total RNA. In vitro transcription was performed to produce biotin-labeled cRNA by using a BioArray HighYield RNA Transcript Labeling Kit (Affymetrix) according to the manufacturer’s instructions (Ishii et al, Genomics 68:136-143, 2000).
| | Sample_hyb_protocol | Array hybridization was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
| | Sample_scan_protocol | Array scanning was performed as described previously (Ishii et al, Genomics 68:136-143, 2000)
| | Sample_data_processing | Microarray Suite software 5.0 of GCOS (Affymetrix) was used with target intensity of 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daizo,,Koinuma
| | Sample_contact_email | d-koinuma@umin.ac.jp
| | Sample_contact_department | Molecular Pathology
| | Sample_contact_institute | University of Tokyo
| | Sample_contact_address | Hongo 7-3-1, Bunkyo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM684nnn/GSM684739/suppl/GSM684739.CEL.gz
| | Sample_series_id | GSE27631
| | Sample_series_id | GSE27661
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
