Search results for the GEO ID: GSE27670  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM685339 | GPL570 |
|
GC B cell_pCDNA3_rep1
|
GC B cell transfected with empty vector (pCDNA3.1), rep1
|
transfection: empty vector (pCDNA3.1)
cell type: GC B cells sorted using CD10 MACS from reactive human tonsils
|
Amplified total RNA from transfected and FACS sorted GC B cells
|
| Sample_geo_accession | GSM685339
| | Sample_status | Public on Jul 27 2011
| | Sample_submission_date | Mar 03 2011
| | Sample_last_update_date | Jul 27 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; CRUK Cancer Centre, Birmingham
| | Sample_treatment_protocol_ch1 | 1 x E6 CD10+ GC B cells were co-transfected with pcDNA3.1 vector together with pMACS LNGFR vector (Miltenyi Biotec Ltd., Surrey, UK) at ratio 7:3. Transfection was performed by Nucleofection using the B Cell Nucleofector Kit B (Amaxa, Cologne, Germany) for the Nucleofector II 6 Device (Amaxa) using U-15 program. Cells were cultivated overnight, stained with α-LNGFR-allophycocyanin (APC) (Miltenyi Biotec) and propidium iodide (PI). APC-labelled and PI-negative cells were collected by FACS on a MoFlo sorter (Dako Cytomation, Colorado, USA).
| | Sample_growth_protocol_ch1 | GC B cells were cultivated overnight post-transfection in RPMI supplemented with 20% FCS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Micro Kit, Qiagen, and RNA was amplified with ExpressArt mRNA Amplification kit protocol (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix labelling protocol
| | Sample_hyb_protocol | Affymetrix hybridization protocol onto Human Genome U133 Plus 2.0 Array
| | Sample_scan_protocol | Affymetrix Standard protocol
| | Sample_data_processing | GCOS
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM685nnn/GSM685339/suppl/GSM685339_Tonsil_1_pCDNA3_RNA_230707.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM685nnn/GSM685339/suppl/GSM685339_Tonsil_1_pCDNA3_RNA_230707.mas5.CHP.gz
| | Sample_series_id | GSE27670
| | Sample_data_row_count | 54675
| | |
|
GSM685340 | GPL570 |
|
GC B cell_pCDNA3_rep2
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GC B cell transfected with empty vector (pCDNA3.1), rep2
|
transfection: empty vector (pCDNA3.1)
cell type: GC B cells sorted using CD10 MACS from reactive human tonsils
|
Amplified total RNA from transfected and FACS sorted GC B cells
|
| Sample_geo_accession | GSM685340
| | Sample_status | Public on Jul 27 2011
| | Sample_submission_date | Mar 03 2011
| | Sample_last_update_date | Jul 27 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; CRUK Cancer Centre, Birmingham
| | Sample_treatment_protocol_ch1 | 1 x E6 CD10+ GC B cells were co-transfected with pcDNA3.1 vector together with pMACS LNGFR vector (Miltenyi Biotec Ltd., Surrey, UK) at ratio 7:3. Transfection was performed by Nucleofection using the B Cell Nucleofector Kit B (Amaxa, Cologne, Germany) for the Nucleofector II 6 Device (Amaxa) using U-15 program. Cells were cultivated overnight, stained with α-LNGFR-allophycocyanin (APC) (Miltenyi Biotec) and propidium iodide (PI). APC-labelled and PI-negative cells were collected by FACS on a MoFlo sorter (Dako Cytomation, Colorado, USA).
| | Sample_growth_protocol_ch1 | GC B cells were cultivated overnight post-transfection in RPMI supplemented with 20% FCS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Micro Kit, Qiagen, and RNA was amplified with ExpressArt mRNA Amplification kit protocol (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix labelling protocol
| | Sample_hyb_protocol | Affymetrix hybridization protocol onto Human Genome U133 Plus 2.0 Array
| | Sample_scan_protocol | Affymetrix Standard protocol
| | Sample_data_processing | GCOS
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM685nnn/GSM685340/suppl/GSM685340_Tonsil_2_pCDNA3_RNA_250707.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM685nnn/GSM685340/suppl/GSM685340_Tonsil_2_pCDNA3_RNA_250707.mas5.CHP.gz
| | Sample_series_id | GSE27670
| | Sample_data_row_count | 54675
| | |
|
GSM685345 | GPL570 |
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GC B cell_PRDM1α_rep1
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GC B cell transfected with pcDNA3.1-PRDM1α, rep1
|
transfection: pcDNA3.1-PRDM1α
cell type: GC B cells sorted using CD10 MACS from reactive human tonsils
|
Amplified total RNA from transfected and FACS sorted GC B cells
|
| Sample_geo_accession | GSM685345
| | Sample_status | Public on Jul 27 2011
| | Sample_submission_date | Mar 03 2011
| | Sample_last_update_date | Jul 27 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; CRUK Cancer Centre, Birmingham
| | Sample_treatment_protocol_ch1 | 1 x E6 CD10+ GC B cells were co-transfected with pcDNA3.1-BLIMP1α vector together with pMACS LNGFR vector (Miltenyi Biotec Ltd., Surrey, UK) at ratio 7:3. Transfection was performed by Nucleofection using the B Cell Nucleofector Kit B (Amaxa, Cologne, Germany) for the Nucleofector II 6 Device (Amaxa) using U-15 program. Cells were cultivated overnight, stained with α-LNGFR-allophycocyanin (APC) (Miltenyi Biotec) and propidium iodide (PI). APC-labelled and PI-negative cells were collected by FACS on a MoFlo sorter (Dako Cytomation, Colorado, USA).
| | Sample_growth_protocol_ch1 | GC B cells were cultivated overnight post-transfection in RPMI supplemented with 20% FCS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Micro Kit, Qiagen, and RNA was amplified with ExpressArt mRNA Amplification kit protocol (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix labelling protocol
| | Sample_hyb_protocol | Affymetrix hybridization protocol onto Human Genome U133 Plus 2.0 Array
| | Sample_scan_protocol | Affymetrix Standard protocol
| | Sample_data_processing | GCOS
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM685nnn/GSM685345/suppl/GSM685345_Tonsil_1_pCDNA3_BLIMP1_RNA_230707.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM685nnn/GSM685345/suppl/GSM685345_Tonsil_1_pCDNA3_BLIMP1_RNA_230707.mas5.CHP.gz
| | Sample_series_id | GSE27670
| | Sample_data_row_count | 54675
| | |
|
GSM685346 | GPL570 |
|
GC B cell_PRDM1α_rep2
|
GC B cell transfected with pcDNA3.1-PRDM1α, rep2
|
transfection: pcDNA3.1-PRDM1α
cell type: GC B cells sorted using CD10 MACS from reactive human tonsils
|
Amplified total RNA from transfected and FACS sorted GC B cells
|
| Sample_geo_accession | GSM685346
| | Sample_status | Public on Jul 27 2011
| | Sample_submission_date | Mar 03 2011
| | Sample_last_update_date | Jul 27 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; CRUK Cancer Centre, Birmingham
| | Sample_treatment_protocol_ch1 | 1 x E6 CD10+ GC B cells were co-transfected with pcDNA3.1-BLIMP1α vector together with pMACS LNGFR vector (Miltenyi Biotec Ltd., Surrey, UK) at ratio 7:3. Transfection was performed by Nucleofection using the B Cell Nucleofector Kit B (Amaxa, Cologne, Germany) for the Nucleofector II 6 Device (Amaxa) using U-15 program. Cells were cultivated overnight, stained with α-LNGFR-allophycocyanin (APC) (Miltenyi Biotec) and propidium iodide (PI). APC-labelled and PI-negative cells were collected by FACS on a MoFlo sorter (Dako Cytomation, Colorado, USA).
| | Sample_growth_protocol_ch1 | GC B cells were cultivated overnight post-transfection in RPMI supplemented with 20% FCS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Micro Kit, Qiagen, and RNA was amplified with ExpressArt mRNA Amplification kit protocol (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix labelling protocol
| | Sample_hyb_protocol | Affymetrix hybridization protocol onto Human Genome U133 Plus 2.0 Array
| | Sample_scan_protocol | Affymetrix Standard protocol
| | Sample_data_processing | GCOS
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM685nnn/GSM685346/suppl/GSM685346_Tonsil_2_pCDNA3_BLIMP1_RNA_250707.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM685nnn/GSM685346/suppl/GSM685346_Tonsil_2_pCDNA3_BLIMP1_RNA_250707.mas5.CHP.gz
| | Sample_series_id | GSE27670
| | Sample_data_row_count | 54675
| | |
|
GSM685379 | GPL570 |
|
GC B cell_pCDNA3_LMP1_rep1
|
GC B cell transfected with pcDNA3.1-LMP1, rep1
|
transfection: pcDNA3.1-LMP1
cell type: GC B cells sorted using CD10 MACS from reactive human tonsils
|
Amplified total RNA from transfected and FACS sorted GC B cells
|
| Sample_geo_accession | GSM685379
| | Sample_status | Public on Jul 27 2011
| | Sample_submission_date | Mar 03 2011
| | Sample_last_update_date | Jul 27 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; CRUK Cancer Centre, Birmingham
| | Sample_treatment_protocol_ch1 | 1 x E6 CD10+ GC B cells were co-transfected with pcDNA3.1-LMP1 vector together with pMACS LNGFR vector (Miltenyi Biotec Ltd., Surrey, UK) at ratio 7:3. Transfection was performed by Nucleofection using the B Cell Nucleofector Kit B (Amaxa, Cologne, Germany) for the Nucleofector II 6 Device (Amaxa) using U-15 program. Cells were cultivated overnight, stained with α-LNGFR-allophycocyanin (APC) (Miltenyi Biotec) and propidium iodide (PI). APC-labelled and PI-negative cells were collected by FACS on a MoFlo sorter (Dako Cytomation, Colorado, USA).
| | Sample_growth_protocol_ch1 | GC B cells were cultivated overnight post-transfection in RPMI supplemented with 20% FCS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Micro Kit, Qiagen, and RNA was amplified with ExpressArt mRNA Amplification kit protocol (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix labelling protocol
| | Sample_hyb_protocol | Affymetrix hybridization protocol onto Human Genome U133 Plus 2.0 Array
| | Sample_scan_protocol | Affymetrix Standard protocol
| | Sample_data_processing | GCOS
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM685nnn/GSM685379/suppl/GSM685379_1_Tonsil_1_pCDNA3_LMP1_RNA_230707.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM685nnn/GSM685379/suppl/GSM685379_Tonsil_1_pCDNA3_LMP1_RNA_230707.mas5.CHP.gz
| | Sample_series_id | GSE27670
| | Sample_data_row_count | 54675
| | |
|
GSM685380 | GPL570 |
|
GC B cell_pCDNA3_LMP1_rep2
|
GC B cell transfected with pcDNA3.1-LMP1, rep2
|
transfection: pcDNA3.1-LMP1
cell type: GC B cells sorted using CD10 MACS from reactive human tonsils
|
Amplified total RNA from transfected and FACS sorted GC B cells
|
| Sample_geo_accession | GSM685380
| | Sample_status | Public on Jul 27 2011
| | Sample_submission_date | Mar 03 2011
| | Sample_last_update_date | Jul 27 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; CRUK Cancer Centre, Birmingham
| | Sample_treatment_protocol_ch1 | 1 x E6 CD10+ GC B cells were co-transfected with pcDNA3.1-LMP1 vector together with pMACS LNGFR vector (Miltenyi Biotec Ltd., Surrey, UK) at ratio 7:3. Transfection was performed by Nucleofection using the B Cell Nucleofector Kit B (Amaxa, Cologne, Germany) for the Nucleofector II 6 Device (Amaxa) using U-15 program. Cells were cultivated overnight, stained with α-LNGFR-allophycocyanin (APC) (Miltenyi Biotec) and propidium iodide (PI). APC-labelled and PI-negative cells were collected by FACS on a MoFlo sorter (Dako Cytomation, Colorado, USA).
| | Sample_growth_protocol_ch1 | GC B cells were cultivated overnight post-transfection in RPMI supplemented with 20% FCS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Micro Kit, Qiagen, and RNA was amplified with ExpressArt mRNA Amplification kit protocol (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix labelling protocol
| | Sample_hyb_protocol | Affymetrix hybridization protocol onto Human Genome U133 Plus 2.0 Array
| | Sample_scan_protocol | Affymetrix Standard protocol
| | Sample_data_processing | GCOS
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM685nnn/GSM685380/suppl/GSM685380_Tonsil_2_pCDNA3_LMP1_RNA_250707.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM685nnn/GSM685380/suppl/GSM685380_Tonsil_2_pCDNA3_LMP1_RNA_250707.mas5.CHP.gz
| | Sample_series_id | GSE27670
| | Sample_data_row_count | 54675
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