Search results for the GEO ID: GSE27718  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM686340 | GPL570 |
|
4L melanoma cell line-scrambled-treated-sample repeat 1
|
Total RNA of 50nM scrambled-transfected cells for 48h
|
cell type: human melanoma cell line 4L
treatment group: 50nM scrambled-transfected for 48h
|
|
| Sample_geo_accession | GSM686340
| | Sample_status | Public on Mar 08 2011
| | Sample_submission_date | Mar 07 2011
| | Sample_last_update_date | Mar 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 250,000 human melanoma cell lines (4L or 5B1) were transiently transfected ,using lipofectamine, with 50nM of scrambled or miR-30d oligos for 48 hours
| | Sample_growth_protocol_ch1 | human melanoma cell lines (4L or 5B1) weregrown in RPMI supplemented with 5% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy micro-elute columns (QIAGEN), according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total cellular RNA was converted to cDNA/cRNA and labeled using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | 10 micrograms of cRNA generated by the IVT procedure was hybridized for 16 hr to the Affymetrix array according to the manufacturer's manual. Genechips® were processed using protocol EukGE_WS2v4 450 for Genechip® fluidics station 450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686340/suppl/GSM686340.CEL.gz
| | Sample_series_id | GSE27718
| | Sample_data_row_count | 54675
| | |
|
GSM686341 | GPL570 |
|
4L melanoma cell line-scrambled-treated-sample repeat 2
|
Total RNA of 50nM scrambled-transfected cells for 48h
|
cell type: human melanoma cell line 4L
treatment group: 50nM scrambled-transfected for 48h
|
|
| Sample_geo_accession | GSM686341
| | Sample_status | Public on Mar 08 2011
| | Sample_submission_date | Mar 07 2011
| | Sample_last_update_date | Mar 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 250,000 human melanoma cell lines (4L or 5B1) were transiently transfected ,using lipofectamine, with 50nM of scrambled or miR-30d oligos for 48 hours
| | Sample_growth_protocol_ch1 | human melanoma cell lines (4L or 5B1) weregrown in RPMI supplemented with 5% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy micro-elute columns (QIAGEN), according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total cellular RNA was converted to cDNA/cRNA and labeled using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | 10 micrograms of cRNA generated by the IVT procedure was hybridized for 16 hr to the Affymetrix array according to the manufacturer's manual. Genechips® were processed using protocol EukGE_WS2v4 450 for Genechip® fluidics station 450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686341/suppl/GSM686341.CEL.gz
| | Sample_series_id | GSE27718
| | Sample_data_row_count | 54675
| | |
|
GSM686342 | GPL570 |
|
4L melanoma cell line-miR-30d-treated-sample repeat 1
|
Total RNA of 50nM miR-30d-transfected cells for 48h
|
cell type: human melanoma cell line 4L
treatment group: 50nM miR-30d-transfected for 48h
|
|
| Sample_geo_accession | GSM686342
| | Sample_status | Public on Mar 08 2011
| | Sample_submission_date | Mar 07 2011
| | Sample_last_update_date | Mar 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 250,000 human melanoma cell lines (4L or 5B1) were transiently transfected ,using lipofectamine, with 50nM of scrambled or miR-30d oligos for 48 hours
| | Sample_growth_protocol_ch1 | human melanoma cell lines (4L or 5B1) weregrown in RPMI supplemented with 5% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy micro-elute columns (QIAGEN), according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total cellular RNA was converted to cDNA/cRNA and labeled using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | 10 micrograms of cRNA generated by the IVT procedure was hybridized for 16 hr to the Affymetrix array according to the manufacturer's manual. Genechips® were processed using protocol EukGE_WS2v4 450 for Genechip® fluidics station 450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686342/suppl/GSM686342.CEL.gz
| | Sample_series_id | GSE27718
| | Sample_data_row_count | 54675
| | |
|
GSM686343 | GPL570 |
|
4L melanoma cell line-miR-30d- treated-sample repeat 2
|
Total RNA of 50nM miR-30d-transfected cells for 48h
|
cell type: human melanoma cell line 4L
treatment group: 50nM miR-30d-transfected for 48h
|
|
| Sample_geo_accession | GSM686343
| | Sample_status | Public on Mar 08 2011
| | Sample_submission_date | Mar 07 2011
| | Sample_last_update_date | Mar 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 250,000 human melanoma cell lines (4L or 5B1) were transiently transfected ,using lipofectamine, with 50nM of scrambled or miR-30d oligos for 48 hours
| | Sample_growth_protocol_ch1 | human melanoma cell lines (4L or 5B1) weregrown in RPMI supplemented with 5% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy micro-elute columns (QIAGEN), according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total cellular RNA was converted to cDNA/cRNA and labeled using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | 10 micrograms of cRNA generated by the IVT procedure was hybridized for 16 hr to the Affymetrix array according to the manufacturer's manual. Genechips® were processed using protocol EukGE_WS2v4 450 for Genechip® fluidics station 450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686343/suppl/GSM686343.CEL.gz
| | Sample_series_id | GSE27718
| | Sample_data_row_count | 54675
| | |
|
GSM686344 | GPL570 |
|
5B1 melanoma cell line -scrambled-treated-sample repeat 1
|
Total RNA of 50nM scrambled-transfected cells for 48h
|
cell type: human melanoma cell line 5B1
treatment group: 50nM scrambled-transfected for 48h
|
|
| Sample_geo_accession | GSM686344
| | Sample_status | Public on Mar 08 2011
| | Sample_submission_date | Mar 07 2011
| | Sample_last_update_date | Mar 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 250,000 human melanoma cell lines (4L or 5B1) were transiently transfected ,using lipofectamine, with 50nM of scrambled or miR-30d oligos for 48 hours
| | Sample_growth_protocol_ch1 | human melanoma cell lines (4L or 5B1) weregrown in RPMI supplemented with 5% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy micro-elute columns (QIAGEN), according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total cellular RNA was converted to cDNA/cRNA and labeled using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | 10 micrograms of cRNA generated by the IVT procedure was hybridized for 16 hr to the Affymetrix array according to the manufacturer's manual. Genechips® were processed using protocol EukGE_WS2v4 450 for Genechip® fluidics station 450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686344/suppl/GSM686344.CEL.gz
| | Sample_series_id | GSE27718
| | Sample_data_row_count | 54675
| | |
|
GSM686345 | GPL570 |
|
5B1 melanoma cell line-scrambled-treated-sample repeat 2
|
Total RNA of 50nM scrambled-transfected cells for 48h
|
cell type: human melanoma cell line 5B1
treatment group: 50nM scrambled-transfected for 48h
|
|
| Sample_geo_accession | GSM686345
| | Sample_status | Public on Mar 08 2011
| | Sample_submission_date | Mar 07 2011
| | Sample_last_update_date | Mar 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 250,000 human melanoma cell lines (4L or 5B1) were transiently transfected ,using lipofectamine, with 50nM of scrambled or miR-30d oligos for 48 hours
| | Sample_growth_protocol_ch1 | human melanoma cell lines (4L or 5B1) weregrown in RPMI supplemented with 5% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy micro-elute columns (QIAGEN), according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total cellular RNA was converted to cDNA/cRNA and labeled using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | 10 micrograms of cRNA generated by the IVT procedure was hybridized for 16 hr to the Affymetrix array according to the manufacturer's manual. Genechips® were processed using protocol EukGE_WS2v4 450 for Genechip® fluidics station 450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686345/suppl/GSM686345.CEL.gz
| | Sample_series_id | GSE27718
| | Sample_data_row_count | 54675
| | |
|
GSM686346 | GPL570 |
|
5B1 melanoma cell line-miR-30d- treated-sample repeat 1
|
Total RNA of 50nM miR-30d-transfected cells for 48h
|
cell type: human melanoma cell line 5B1
treatment group: 50nM miR-30d-transfected for 48h
|
|
| Sample_geo_accession | GSM686346
| | Sample_status | Public on Mar 08 2011
| | Sample_submission_date | Mar 07 2011
| | Sample_last_update_date | Mar 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 250,000 human melanoma cell lines (4L or 5B1) were transiently transfected ,using lipofectamine, with 50nM of scrambled or miR-30d oligos for 48 hours
| | Sample_growth_protocol_ch1 | human melanoma cell lines (4L or 5B1) weregrown in RPMI supplemented with 5% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy micro-elute columns (QIAGEN), according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total cellular RNA was converted to cDNA/cRNA and labeled using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | 10 micrograms of cRNA generated by the IVT procedure was hybridized for 16 hr to the Affymetrix array according to the manufacturer's manual. Genechips® were processed using protocol EukGE_WS2v4 450 for Genechip® fluidics station 450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686346/suppl/GSM686346.CEL.gz
| | Sample_series_id | GSE27718
| | Sample_data_row_count | 54675
| | |
|
GSM686347 | GPL570 |
|
5B1 melanoma cell line-miR-30d- treated-sample repeat 2
|
Total RNA of 50nM miR-30d-transfected cells for 48h
|
cell type: human melanoma cell line 5B1
treatment group: 50nM miR-30d-transfected for 48h
|
|
| Sample_geo_accession | GSM686347
| | Sample_status | Public on Mar 08 2011
| | Sample_submission_date | Mar 07 2011
| | Sample_last_update_date | Mar 08 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 250,000 human melanoma cell lines (4L or 5B1) were transiently transfected ,using lipofectamine, with 50nM of scrambled or miR-30d oligos for 48 hours
| | Sample_growth_protocol_ch1 | human melanoma cell lines (4L or 5B1) weregrown in RPMI supplemented with 5% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy micro-elute columns (QIAGEN), according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total cellular RNA was converted to cDNA/cRNA and labeled using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | 10 micrograms of cRNA generated by the IVT procedure was hybridized for 16 hr to the Affymetrix array according to the manufacturer's manual. Genechips® were processed using protocol EukGE_WS2v4 450 for Genechip® fluidics station 450.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686347/suppl/GSM686347.CEL.gz
| | Sample_series_id | GSE27718
| | Sample_data_row_count | 54675
| | |
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