Search results for the GEO ID: GSE27785  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM686636 | GPL1261 |
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CD34(-)KSL hematopoietic stem cells, biological replicate 1
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CD34(-)KSL hematopoietic stem cells
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cell type: CD34(-)KSL hematopoietic stem cells
genetic background: C57BL/6
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CD34(-)KSL hematopoietic stem cells purified by cell sorting
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| Sample_geo_accession | GSM686636
| | Sample_status | Public on May 19 2011
| | Sample_submission_date | Mar 07 2011
| | Sample_last_update_date | May 19 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using an ISOGEN reagent (Nippon Gene).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from the purified total RNA equivalent to 3,000 cells with a Two-Cycle cDNA Synthesis Kit and 3'-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hitoshi,,Ichikawa
| | Sample_contact_email | hichikaw@ncc.go.jp
| | Sample_contact_phone | +81-3-3542-2511
| | Sample_contact_fax | +81-3-3248-1631
| | Sample_contact_laboratory | Genetics Division
| | Sample_contact_institute | National Cancer Center Research Institute
| | Sample_contact_address | 5-1-1 Tsukiji, Chuo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 104-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686636/suppl/GSM686636_CD34_-_KSL_3-S.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686636/suppl/GSM686636_CD34_-_KSL_3.CEL.gz
| | Sample_series_id | GSE27785
| | Sample_series_id | GSE27787
| | Sample_data_row_count | 45101
| | |
|
GSM686637 | GPL1261 |
|
CD34(-)KSL hematopoietic stem cells, biological replicate 2
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CD34(-)KSL hematopoietic stem cells
|
cell type: CD34(-)KSL hematopoietic stem cells
genetic background: C57BL/6
|
CD34(-)KSL hematopoietic stem cells purified by cell sorting
|
| Sample_geo_accession | GSM686637
| | Sample_status | Public on May 19 2011
| | Sample_submission_date | Mar 07 2011
| | Sample_last_update_date | May 19 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using an ISOGEN reagent (Nippon Gene).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from the purified total RNA equivalent to 3,000 cells with a Two-Cycle cDNA Synthesis Kit and 3'-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hitoshi,,Ichikawa
| | Sample_contact_email | hichikaw@ncc.go.jp
| | Sample_contact_phone | +81-3-3542-2511
| | Sample_contact_fax | +81-3-3248-1631
| | Sample_contact_laboratory | Genetics Division
| | Sample_contact_institute | National Cancer Center Research Institute
| | Sample_contact_address | 5-1-1 Tsukiji, Chuo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 104-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686637/suppl/GSM686637_CD34_-_KSL_4-S.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686637/suppl/GSM686637_CD34_-_KSL_4.CEL.gz
| | Sample_series_id | GSE27785
| | Sample_series_id | GSE27787
| | Sample_data_row_count | 45101
| | |
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GSM686638 | GPL1261 |
|
CD34(+)KSL multipotent progenitors, biological replicate 1
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CD34(+)KSL multipotent progenitors
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cell type: CD34(+)KSL multipotent progenitors
genetic background: C57BL/6
|
CD34(+)KSL multipotent progenitors purified by cell sorting
|
| Sample_geo_accession | GSM686638
| | Sample_status | Public on May 19 2011
| | Sample_submission_date | Mar 07 2011
| | Sample_last_update_date | May 19 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using an ISOGEN reagent (Nippon Gene).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from the purified total RNA equivalent to 3,000 cells with a Two-Cycle cDNA Synthesis Kit and 3'-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hitoshi,,Ichikawa
| | Sample_contact_email | hichikaw@ncc.go.jp
| | Sample_contact_phone | +81-3-3542-2511
| | Sample_contact_fax | +81-3-3248-1631
| | Sample_contact_laboratory | Genetics Division
| | Sample_contact_institute | National Cancer Center Research Institute
| | Sample_contact_address | 5-1-1 Tsukiji, Chuo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 104-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686638/suppl/GSM686638_CD34_+_KSL_3-S.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686638/suppl/GSM686638_CD34_+_KSL_3.CEL.gz
| | Sample_series_id | GSE27785
| | Sample_series_id | GSE27787
| | Sample_data_row_count | 45101
| | |
|
GSM686639 | GPL1261 |
|
CD34(+)KSL multipotent progenitors, biological replicate 2
|
CD34(+)KSL multipotent progenitors
|
cell type: CD34(+)KSL multipotent progenitors
genetic background: C57BL/6
|
CD34(+)KSL multipotent progenitors purified by cell sorting
|
| Sample_geo_accession | GSM686639
| | Sample_status | Public on May 19 2011
| | Sample_submission_date | Mar 07 2011
| | Sample_last_update_date | May 19 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using an ISOGEN reagent (Nippon Gene).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from the purified total RNA equivalent to 3,000 cells with a Two-Cycle cDNA Synthesis Kit and 3'-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Hitoshi,,Ichikawa
| | Sample_contact_email | hichikaw@ncc.go.jp
| | Sample_contact_phone | +81-3-3542-2511
| | Sample_contact_fax | +81-3-3248-1631
| | Sample_contact_laboratory | Genetics Division
| | Sample_contact_institute | National Cancer Center Research Institute
| | Sample_contact_address | 5-1-1 Tsukiji, Chuo-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 104-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686639/suppl/GSM686639_CD34_+_KSL_4-S.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM686nnn/GSM686639/suppl/GSM686639_CD34_+_KSL_4.CEL.gz
| | Sample_series_id | GSE27785
| | Sample_series_id | GSE27787
| | Sample_data_row_count | 45101
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